Difference between revisions of "Free Radical Generation"
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For Cerium Free Radicals: | For Cerium Free Radicals: | ||
100 mL solution of 2 mM HPA and 100 mL solution of Ce (IV). | 100 mL solution of 2 mM HPA and 100 mL solution of Ce (IV). | ||
| + | |||
For 100 mL of 2 mM HPA: | For 100 mL of 2 mM HPA: | ||
| + | |||
Weigh out 30.4mg of HPA | Weigh out 30.4mg of HPA | ||
| + | |||
Add it into 100 mL DI water | Add it into 100 mL DI water | ||
| + | |||
For 100 mL of 2 mM Ce (IV) | For 100 mL of 2 mM Ce (IV) | ||
| + | |||
Weigh out 109.6 mg of Ammonium Cerium Nitrate | Weigh out 109.6 mg of Ammonium Cerium Nitrate | ||
| + | |||
Add that to 2.5 mL of 18M sulfuric acid | Add that to 2.5 mL of 18M sulfuric acid | ||
| + | |||
Add that solution to 97.5 mL of DI water | Add that solution to 97.5 mL of DI water | ||
Revision as of 21:13, 14 April 2016
The 1-electron oxidation of a phenol can be accomplished via chemical or enzymatic means.
Chemical Means
1) KMnO4
2. Cerium (IV)
For Cerium Free Radicals: 100 mL solution of 2 mM HPA and 100 mL solution of Ce (IV).
For 100 mL of 2 mM HPA:
Weigh out 30.4mg of HPA
Add it into 100 mL DI water
For 100 mL of 2 mM Ce (IV)
Weigh out 109.6 mg of Ammonium Cerium Nitrate
Add that to 2.5 mL of 18M sulfuric acid
Add that solution to 97.5 mL of DI water