Difference between revisions of "Pipette Verification"
| Line 1: | Line 1: | ||
Pipettes are commonly used in the biochemistry laboratory and in most cases, users make the assumption that these "instruments" are fully functional, but this is not always the case. Pipettes are dropped or mistreated and this can result in the pipette requiring recalibration. At the beginning of any research project, it is highly recommended that you "verify" that the pipette is working properly. An extensive discussion of this can be found [[Media:Verification Procedure for Accuracy and Precision.pdf|here]]...but for research purposes, the verification procedure can be significantly less involved. | Pipettes are commonly used in the biochemistry laboratory and in most cases, users make the assumption that these "instruments" are fully functional, but this is not always the case. Pipettes are dropped or mistreated and this can result in the pipette requiring recalibration. At the beginning of any research project, it is highly recommended that you "verify" that the pipette is working properly. An extensive discussion of this can be found [[Media:Verification Procedure for Accuracy and Precision.pdf|here]]...but for research purposes, the verification procedure can be significantly less involved. | ||
| − | Before beginning the validation process, we must acknowledge the concepts of accuracy and precision. | + | Before beginning the validation process, we must acknowledge the concepts of ''accuracy'' and ''precision''. |
| − | : | + | |
| + | Below are the "target grouping" description of accuracy and precision: | ||
| + | {| | ||
| + | | | ||
| + | [[File:File:High accuracy Low precision.png|200px|thumb|left|high accuracy low precision]] | ||
| + | || | ||
| + | [[File:File:High precision Low accuracy.png|200px|thumb|left|low accuracy high precision]] | ||
| + | |} | ||
| − | |||
File:Accuracy and precision.png | File:Accuracy and precision.png | ||
| − | + | ||
| − | + | ||
Revision as of 10:29, 30 May 2017
Pipettes are commonly used in the biochemistry laboratory and in most cases, users make the assumption that these "instruments" are fully functional, but this is not always the case. Pipettes are dropped or mistreated and this can result in the pipette requiring recalibration. At the beginning of any research project, it is highly recommended that you "verify" that the pipette is working properly. An extensive discussion of this can be found here...but for research purposes, the verification procedure can be significantly less involved.
Before beginning the validation process, we must acknowledge the concepts of accuracy and precision.
Below are the "target grouping" description of accuracy and precision:
|
File:File:High accuracy Low precision.png high accuracy low precision |
File:File:High precision Low accuracy.png low accuracy high precision |
File:Accuracy and precision.png
that there are two sources of error:
- 1) Systematic
- 2) Instrumental
Recommended Verification Procedure using Gravimetric Test