Methods include Qiagen DNA isolation kits

Qiagen Kit Overview

QIAGEN offers molecular sample and assay technology. Some of these technologies are provided as kits. These kits utilize spin columns to isolate DNA and RNA.

DNA Extraction Lysis Buffer breaks down the cell membrane to release intercellular materials like DNA, RNA, and other organelles from the cell.

Purification To bind the DNA to the silica spin column, Chaotropic Salts are critical. Salts disrupt Hydrogen bonding between strands and allow the DNA to adhere to the silica column.

Wash Now that the DNA is bound to the column, it needs to be washed. The membrane is still dirty with residual proteins and salt. Washing it will remove the impurities.

Dry After the column is washed it is centrifuged to dry. Drying removes the ethanol and prevents contamination and a low yield.

Elution The final step is releasing the pure DNA from the silica column. This is done by allowing 10mM Tris buffer to sit in the DNA before centrifuging.

DNeasy PowerSoil Pro Kit

The kit produces high yields of microbial DNA from a variety of soil types such as top soil, compost, and clay.

Microbiome Project

QIAamp PowerFecal Pro DNA Kit

Kit produces high yields of microbial DNA from stool and gut samples.

Microbiome Project