Fatty Acid Methyl Esters

Introduction

Archaeology is the study of human societies through the analysis of material remains (1). The analysis of material remains can be done through many different processes and can reveal the purpose and origin of each material. The introduction of analytical chemistry techniques into the field of archaeology has allowed archaeologists to gain an even deeper understanding of any given material. The procedure described in this experiment is one such technique. This experiment uses gas chromatography-mass spectrometry to identify the origins of a variety of fatty acids in a sample. Humans modify the chemical composition of everything around them through daily activities such as collecting and preparing food, the use of specific pigments and dyes, and a variety of other practices (2). The chemical composition of different material remains can therefore tell a story about the people who used these materials. Dried clay used by humans of the past holds lipid residue fairly well. If this clay is pulverized and treated with the chemicals described in this paper, the sample can undergo GC-MS analysis. Through this analytical technique the fragmentation patterns of each oil can be obtained, allowing for the identification of all the oils in the sample. Every type of oil has a specific combination of fatty acids that comprise the chemical makeup of said oil. Comparing fatty acid composition to previously established oil composition data can help identify which types of oil are in the samples. A similar process can be done with modern-day cooking oils to compare their fatty acid oil composition to current oil composition data. This experiment analyzes the fatty acid composition of grapeseed oil, corn oil, and sunflower oil. The fatty acids focused on in this study were palmitic acid, oleic acid, stearic acid, linoleic acid, and linolenic acid.

Fatty acid content can be used to help identify the origins of different types of oils. Each type of oil has a unique amount of different fatty acids. Unsaturated fatty acids are more common in plant materials, while saturated fatty acids are more common in animal fats. Certain plant materials are known to be used for tasks such as dyeing fabrics, so being able to tell what type of plant each pot was used for can determine the purpose of each pot. These values can help archaeologists determine what kind of material a pot was used for if the pot is crushed up and sent in for GC-MS analysis. This is just one of the many applications this fatty acid methyl ester GC-MS analysis technique has in a variety of disciplines. This table shows some experimental values of fatty acid content in different types of oils.

Figure 4. is a table depicting the experimental values of fatty acid content in different types of fats and oils.

Methods and Materials

Materials

Oil Samples

All samples were obtained from Monmouth College's nutrition laboratory. The grapeseed oil was the brand Carlini. The sunflower oil was virgin sunflower oil of the brand Authentic Menu. The corn oil was "pure" corn oil of the brand Essential Everyday.

Chemicals

Methanol, KOH, pentane, and acetic acid were all purchased from Millipore Sigma.

Sample Preparation/Trasesterifcation

Before an oil sample can be detected by GC-MS analysis, the fatty acids must be methylated to increase their volatility. The process by which this is done is called transesterification. The methyl from the methanol group is added to the carboxylic acid of the fatty acid to create a fatty acid methyl ester.

Figure 5. shows the transesterification process of linoleic acid to linoleic acid methyl ester.

The following images depict the chemical structures of the fatty acid methyl esters that have been detected in the samples. These images were created using ChemSketch.

Figure 1. shows the chemical structures of palmitic acid methyl ester and stearic acid methyl ester

Figure 2. shows the chemical structure of oleic acid methyl ester.

Figure 3. shows the chemical structure of linoleic acid methyl ester and linolenic acid methyl ester.

200 ul of each sample was combined with 40 ul of anhydrous methanol into a microcentrifuge tube. Then 4.3 ul of 5 M KOH was added to the top methanol layer to act as a catalyst for the reaction. The microcentrifuge tubes were then sonicated in a Fisher Scientific model FS30H ultrasonic bath for 30 minutes. After being removed from the bath, 200 ul of 0.1 M acetic acid was added to each microcentrifuge tube to neutralize the base catalyst. Then each tube was centrifuged at 6,000 rpm for 5 minutes in a Capp Denmark model CR-68X tabletop microcentrifuge. After centrifugation, 40 ul of the top layer of each solution was removed and added to a GC vial. Each vial was then diluted with 40 ul of pentane for GC-MS analysis.

Results

GC-MS Analysis

GC-MS Method for the Analysis of Fatty Acid Methyl Esters:

Grapeseed Oil Data

Grapeseed oil was treated as described above. The transesterifcation products were palmitic acid, linoleic acid, and oleic acid. The oleic acid peak was much smaller than expected. While we have no definitive answer as to why this peak was so small, one theory is that the grapeseed oil was old and had undergone oxidation reactions.

Figure 6. The transesterification products of grapeseed oil: palmitic acid, linoleic acid ,and oleic acid.

Figure 7. The mass spectrum of the palmitic acid peak in the grapeseed oil sample.

Figure 8. The mass spectrum of the linoleic acid peak in the grapeseed oil sample.

Figure 9. The mass spectrum of the oleic acid peak in the grapeseed oil sample.

Corn Oil Data

Corn oil was treated as described above. The transesterification products were palmitic acid, linoleic acid, and an unknown acid. The third peak on the chromatogram should have been an oleic acid peak according to previously recorded fatty acid contents of corn oil. Instead, the third peak is an unknown fatty acid with a fatty acid methyl ester parent ion of 280 m/z. The chromatogram peaks of the corn oil were also much noisier than the peaks in the other chromatograms.

Figure 10. The transesterification products of corn oil: palmitic acid, linoleic acid, and an unidentified acid.

Figure 11. The mass spectrum of the palmitic acid peak in the corn oil sample.

Figure 12. The mass spectrum of the linoleic acid peak in the corn oil sample.

Figure 13. The mass spectrum of the unidentified acid peak in the corn oil sample.

Sunflower Oil Data

Sunflower oil was treated as described above. The transesterifcation products were palmitic acid, oleic acid, and stearic acid. The largest peak on the chromatogram should have been a linoleic acid peak according to previously recorded fatty acid contents of sunflower oil. Instead, the largest peak is oleic acid, which should have had a much lower content. The third peak is also much smaller than previous data would suggest, and is stearic acid. The stearic acid peak should be very small according to previous data. The largest peak appears to be split as well as wider than one would hope for a GC-MS chromatogram. This could be due to a possible overloading of the column with this particular sample.

Figure 14. The transesterification products in the sunflower oil sample: palmitic acid, oleic acid, and stearic acid.

Figure 15. The mass spectrum of the palmitic acid peak in the sunflower oil sample.

Figure 16. The mass spectrum of the oleic acid peak in the sunflower oil sample.

Figure 17. The mass spectrum of the stearic acid peak in the sunflower oil sample.

Discussion

The results of this experiment show that this technique for analyzing fatty acid methyl-esters in oil samples will produce similar to expected values. The issues with some of the peaks representing different acids could be due to oxidation that happened within the oils because of the age of the oils. The corn oil chromatogram peaks were very noisy compared to the other chromatograms. A repeat of this experiment with new oils could eliminate the possibility of this causing issues in the experiment. Another recommendation for this experiment would be to use a slightly larger volume of samples for GC-MS analysis to prevent loss of a great amount of sample due to evaporation.

References

Lipid Residue Analysis of Archaeological Pottery: An Introductory Laboratory Experiment in Archaeological Chemistry Clare S. Harper, Faith V. Macdonald, and Kevin L. Braun Journal of Chemical Education 2017 94 (9), 1309-1313 DOI: 10.1021/acs.jchemed.7b00225