Difference between revisions of "Fatty Acid Methyl Esters"
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==Sunflower Oil Data== | ==Sunflower Oil Data== | ||
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| + | ==References== | ||
| + | Lipid Residue Analysis of Archaeological Pottery: An Introductory Laboratory Experiment in Archaeological Chemistry | ||
| + | Clare S. Harper, Faith V. Macdonald, and Kevin L. Braun | ||
| + | Journal of Chemical Education 2017 94 (9), 1309-1313 | ||
| + | DOI: 10.1021/acs.jchemed.7b00225 | ||
Revision as of 01:31, 16 April 2021
Background Information
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Significance of Fatty Acid Methyl Esters
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Transesterification
Image . This reaction is known as transesterification. The methyl from the methanol group is added to the carboxylic acid of the fatty acid to create a fatty acid methyl ester.
Sample Preparation
- Combined 200 ul of the sample with 40 ul of anhydrous methanol into a microcentrifuge tube
- Added 4.3 ul of 5 M KOH to the top methanol layer (catalyst)
- Sonicated the tubes in an ultrasonic bath for 30 minutes
- Added 200 ul of 0.1 M acetic acid (neutralize the base catalyst)
- Centrifuged the solution at 6,000 rpm for 5 minutes
- Transferred 40 ul of the top layer to a GC vial and diluted with pentane
GC-MS Analysis
Grapeseed Oil Data
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Corn Oil Data
Sunflower Oil Data
References
Lipid Residue Analysis of Archaeological Pottery: An Introductory Laboratory Experiment in Archaeological Chemistry Clare S. Harper, Faith V. Macdonald, and Kevin L. Braun Journal of Chemical Education 2017 94 (9), 1309-1313 DOI: 10.1021/acs.jchemed.7b00225