Difference between revisions of "Fatty Acid Methyl Esters"
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| Line 9: | Line 9: | ||
==Sample Preparation== | ==Sample Preparation== | ||
| − | + | *Combined 200 ul of the sample with 40 ul of anhydrous methanol into a microcentrifuge tube | |
| − | + | *Added 4.3 ul of 5 M KOH to the top methanol layer (catalyst) | |
| + | *Sonicated the tubes in an ultrasonic bath for 30 minutes | ||
| + | *Added 200 ul of 0.1 M acetic acid (neutralize the base catalyst) | ||
| + | *Centrifuged the solution at 6,000 rpm for 5 minutes | ||
| + | *Transferred 40 ul of the top layer to a GC vial and diluted with pentane | ||
==GC-MS Analysis== | ==GC-MS Analysis== | ||
Revision as of 02:29, 14 April 2021
Background Information
Significance of Fatty Acid Methyl Esters
Transesterification
Sample Preparation
- Combined 200 ul of the sample with 40 ul of anhydrous methanol into a microcentrifuge tube
- Added 4.3 ul of 5 M KOH to the top methanol layer (catalyst)
- Sonicated the tubes in an ultrasonic bath for 30 minutes
- Added 200 ul of 0.1 M acetic acid (neutralize the base catalyst)
- Centrifuged the solution at 6,000 rpm for 5 minutes
- Transferred 40 ul of the top layer to a GC vial and diluted with pentane