JKRicketts-Hagan Fall 2017

Chemistry/Biochemistry Research 430

Fall 2018

Jeffrey Ricketts-Hagan

Senior Chemistry Major

Research Times

Mon, Fri 6-8pm Wed 2-6pm

section 02 = 0.50 credit = 8 hours per week. Change in progress

Proposed Research Project

Investigating the metabolism of acetaminophen modeled by an enzymatic, single-electron oxidation using HRP and to investigate the chemical properties of the primary products.

Enter Project Title here

Characterization and Analysis of Acetaminophen Metabolites

General Information

Advisor: Brad Sturgeon

Other research student collaborators: Ben C. Stillwell

Other Research Collaborators: here: Ian C.Salveson

Proposal

Determine chemical properties of previously isolated primary acetaminophen oxidation products using cyclic voltammetry and ESR spectroscopy.

Instruments to be used

HPLC, Flash Chromotography System, NMR, Immobilized Enzyme column, ESR

References (2 minimum)

1.) Hinson, Jack A., Dean W. Roberts, and Laura P. James. "Mechanisms of Acetaminophen-Induced Liver Necrosis." Handbook of Experimental Pharmacology Adverse Drug Reactions (2009): 369-405. Web. 2.) Potter, David W., Dwight W. Miller, and Jack A. Hinson. "Identification of Acetaminophen Polymerization Products Catalyzed by Horseradish Peroxidase." Journal of Biological Chemistry 260.22 (1985): 12174-2180. Print.

Research pledge

I, Jeffrey Ricketts-Hagan, have read the Chem/Bioc 430 course syllabus and understand the general structure and expectations of the research program. The above material was prepared after consultation, and in conjunction with my research advisor.

Written Report

...from the course syllabus...

ACS Guide to Research Reports

Research students are expected to write a report and submit it to the research coordinator by the last day of regular semester classes. These reports are intended to summarize the data collected over the course of the semester. Since this research course requires you to enroll in at least two semesters of research, two reports will be generated. The first semester report should be considered a work in progress, where as the final report should be a more comprehensive summary of your research project. These reports are necessary since several researchers may work on each project. The report will be organized so that it is clear what material is new and what material is review. When available, research students will be provided with an electronic copy of the latest report on their project. The research student is expected to update and improve the introduction, background, and literature sections with each report. Results that challenge earlier conclusions will be justified in a discussion section. Reports will be submitted in both electronic and hard copy to the Faculty Research Advisor AND Research Coordinator.

1. Descriptive information

A project title, the names of the researcher (past and current), faculty research advisor, and other relevant student researchers and/or collaborators, the name and page numbers of the lab notebook(s) where the research is described, the dates when the work was done, and the names of the document file and its immediate precursor.

2. Introduction

State of the motivation for the project of interest in terms of current literature. When we make reference to current literature, this is where you provide specific reference(s) to this work. This section can be largely copied from an earlier report(s), if they exist, but it is expected that this section is edited to include newly found background information. Note: if the author of previous report(s) is included as a contributor to the project, you may cut and paste text; this is not plagiarism, it is collaboration.

3. Background from earlier reports

This section will summarize the work reported in earlier reports. If significant results were presented in the most recent report, these results will be summarized and in most cases can be added to that reports background section. This section is included mainly to confirm the current students overall understanding of the project.

4. Experimental

This section describes experiments done during the period covered by the report. Usually, this work will be similar or identical to that described in the prior report, if they exists. Any new experiments will be written up and added to the experimental section. Any experiments preformed under identical conditions need only reference previous reports.

5. Results

This section documents the results of the experiments done during the period covered by the report. Tabulation of data is encouraged; representative spectra presented.

6. Discussion

In this section, the student will discuss the results of their work in context of the literature and the results of earlier reports. Questions that have been raised in earlier reports may be addressed here.

7. Conclusions

Restate the findings of this period of research. The statement “No conclusions have been reached,” is an acceptable statement.

8. Future Directions

In this section, the student will discuss possible experiments intended to address unanswered questions or technical problems on the project.

9. Literature references

Literature referenced in the report will be cited. This will be copied from the earlier report, and the student is expected to contribute to the accumulation of relevant literature. Remember that all cited literature must be read.

10. Signature

Two copies of the report will be signed and dated and turned in to the Faculty Research Advisor and archived by the Research Coordinator.