David Thul Chem430 F16

Chemistry/Biochemistry Research 430

Fall 2016

David Thul

Senior Chemistry Major

Research Times

Mon 2-6 pm

section 01 = 0.25 credit = 4 hours per week.

section 02 = 0.50 credit = 8 hours per week.

Proposed Research Project

Enter Project Title here

Insertion of Amyloid Beta into anionic membranes

General Information

Advisor: Audra Sostarecz

Other research student collaborators: here

Other Research Collaborators: here

Proposal

Looking at binding patterns of amyloid beta (AB) into anionic membranes. With varying the anionic conditions of synthetic membranes, based on the relative concentrations of DPPC and DPPS, the insertion rate into the lipids tail groups and the binding pattern on the head groups will be studied. It is expected to see that binding and insertion patterns be more significant with an increasing anionic concentration. Previous research here has looked at domain characterization of DPPC/DPPS/cholesterol, as well as the binding characteristics of AB in DPPC model membranes. This research aims to establish a stable platform for further researchers, establishing a relationship between membrane charge and AB binding/insertion interactions.

Instruments to be used

Monolayer Trough: isotherm, compression analysis. AFM. Fluorescence spectroscopy.

Research pledge

I, David Thul, have read the Chem/Bioc 430 course syllabus and understand the general structure and expectations of the research program. The above material was prepared after consultation, and in conjunction with my research advisor.