Difference between revisions of "David Thul Chem430 F16"
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==Research Times== | ==Research Times== | ||
| − | Mon 2- | + | Mon 2-6 pm |
: section 01 = 0.25 credit = 4 hours per week. | : section 01 = 0.25 credit = 4 hours per week. | ||
: section 02 = 0.50 credit = 8 hours per week. | : section 02 = 0.50 credit = 8 hours per week. | ||
| Line 18: | Line 18: | ||
===Proposal=== | ===Proposal=== | ||
| + | Looking at binding patterns of amyloid beta (AB) into anionic membranes. With varying the anionic conditions of synthetic membranes, based on the relative concentrations of DPPC and DPPS, the insertion rate into the lipids tail groups and the binding pattern on the head groups will be studied. It is expected to see that binding and insertion patterns be more significant with an increasing anionic concentration. Previous research here has looked at domain characterization of DPPC/DPPS/cholesterol, as well as the binding characteristics of AB in DPPC model membranes. This research aims to establish a stable platform for further researchers, establishing a relationship between membrane charge and AB binding/insertion interactions. | ||
===Instruments to be used=== | ===Instruments to be used=== | ||
| − | Monolayer Trough | + | Monolayer Trough: isotherm, compression analysis. |
| − | AFM | + | AFM. |
| − | Fluorescence | + | Fluorescence spectroscopy. |
===References (2 minimum)=== | ===References (2 minimum)=== | ||
Latest revision as of 19:43, 30 September 2016
Chemistry/Biochemistry Research 430
- Fall 2016
- David Thul
- Senior Chemistry Major
Research Times
Mon 2-6 pm
- section 01 = 0.25 credit = 4 hours per week.
- section 02 = 0.50 credit = 8 hours per week.
Proposed Research Project
Enter Project Title here
Insertion of Amyloid Beta into anionic membranes
General Information
- Advisor: Audra Sostarecz
- Other research student collaborators: here
- Other Research Collaborators: here
Proposal
Looking at binding patterns of amyloid beta (AB) into anionic membranes. With varying the anionic conditions of synthetic membranes, based on the relative concentrations of DPPC and DPPS, the insertion rate into the lipids tail groups and the binding pattern on the head groups will be studied. It is expected to see that binding and insertion patterns be more significant with an increasing anionic concentration. Previous research here has looked at domain characterization of DPPC/DPPS/cholesterol, as well as the binding characteristics of AB in DPPC model membranes. This research aims to establish a stable platform for further researchers, establishing a relationship between membrane charge and AB binding/insertion interactions.
Instruments to be used
Monolayer Trough: isotherm, compression analysis. AFM. Fluorescence spectroscopy.
References (2 minimum)
Research pledge
I, David Thul, have read the Chem/Bioc 430 course syllabus and understand the general structure and expectations of the research program. The above material was prepared after consultation, and in conjunction with my research advisor.