Difference between revisions of "Extraction Method"
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(Created page with "==Procedure== Freeze green coffee beans with liquid nitrogen to minimize degradation of the chlorogenic acid. Crush in a ball mill for 15 mins. Add 1g of gree...") |
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Freeze green coffee beans with liquid nitrogen to minimize degradation of the chlorogenic acid. | Freeze green coffee beans with liquid nitrogen to minimize degradation of the chlorogenic acid. | ||
| − | Crush in a [[Ball_mill|ball mill]] for 15 mins. | + | Crush 100 bean lots in a [[Ball_mill|ball mill]] for 15 mins. |
| − | Add 1g of green coffee bean powder to | + | Add 1g of green coffee bean powder to 250ml erlenmeyer with 100ml methanol/water 70/30 mixture with .5% Na<sub>2</sub>SO<sub>3</sub>. |
| + | |||
| + | Shake the flask over night at 4°C and 125 rpm | ||
| + | |||
| + | Filter through cotton | ||
Latest revision as of 14:55, 20 September 2016
Procedure
Freeze green coffee beans with liquid nitrogen to minimize degradation of the chlorogenic acid.
Crush 100 bean lots in a ball mill for 15 mins.
Add 1g of green coffee bean powder to 250ml erlenmeyer with 100ml methanol/water 70/30 mixture with .5% Na2SO3.
Shake the flask over night at 4°C and 125 rpm
Filter through cotton