Difference between revisions of "Antonetta Axup Chem430 F16"
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==Proposed Research Project== | ==Proposed Research Project== | ||
===Enter Project Title here=== | ===Enter Project Title here=== | ||
| + | An Investigation into the Antibacterial Properties of Essential Oils | ||
===General Information=== | ===General Information=== | ||
| Line 18: | Line 19: | ||
===Proposal=== | ===Proposal=== | ||
| + | Langmuir Monolayers of sweet orange essential oil, the phospholipids dipalmitoylphosphatidylcholine (DPPC), dipalmitoylphosphatidylglycerol (DPPG) and E.coli lipid extract were used to investigate the antibacterial properties of essential oils. The Langmuir Monolayer technique is a useful one for the formation of model cell membranes by allowing analysis of the interactions between the molecules and their organization capabilities. Monolayers using the sweet orange oil as a subphase with DPPC added were found to be more fluid, and to be less stable, as indicated by a low surface pressure at low molecular areas when compared to monolayers with ultra pure water as the subphase. Similarly, monolayers using the sweet orange oil as a subphase with the E. coli lipid extract added were found to be more fluid, and to be less stable, as indicated by a low surface pressure at low molecular areas when compared to monolayers with ultra pure water as the subphase. These results were also found with the trials of DPPG. Further investigations will involve using a simpler gram-negative model, such as 1,2-dipalmitoyl-sn-glycero-3-phospho-L-serine (DPPS), since E. coli is a more complex gram-negative model, and observing those results in the orange oil, changing the concentration of the oil in the subphase and using different essential oils as subphases. | ||
===Instruments to be used=== | ===Instruments to be used=== | ||
| + | Langmuir Monolayer Trough | ||
===References (2 minimum)=== | ===References (2 minimum)=== | ||
| + | :"Antibiotic / Antimicrobial Resistance." Centers for Disease Control and Prevention. Centers for Disease Control and Prevention, 02 Mar. 2016. Web. 19 Apr. 2016. <http://www.cdc.gov/drugresistance/>. | ||
| + | :Boire NA, Riedel S, Parrish NM (2013) Essential Oils and Future Antibiotics: New Weapons against Emerging ‘Superbugs’? J Anc Dis Prev Rem 1:105 | ||
| + | :Nazzaro, Filomena, Florinda Fratianni, Laura De Martino, Raffaele Coppola, and Vincenzo De Feo. "Effect of Essential Oils on Pathogenic Bacteria."Pharmaceuticals 6.12 (2013): 1451-474. Web. | ||
===Research pledge=== | ===Research pledge=== | ||
I, Antonetta Axup, have read the Chem/Bioc 430 course syllabus and understand the general structure and expectations of the research program. The above material was prepared after consultation, and in conjunction with my research advisor. | I, Antonetta Axup, have read the Chem/Bioc 430 course syllabus and understand the general structure and expectations of the research program. The above material was prepared after consultation, and in conjunction with my research advisor. | ||
Latest revision as of 14:18, 28 September 2016
Chemistry/Biochemistry Research 430
- Fall 2016
- Antonetta Axup
- Junior Biochemistry Major
Research Times
T/Thur 2-4 pm
- section 01 = 0.25 credit = 4 hours per week.
- section 02 = 0.50 credit = 8 hours per week.
Proposed Research Project
Enter Project Title here
An Investigation into the Antibacterial Properties of Essential Oils
General Information
- Advisor: Audra Sostarecz
- Other research student collaborators: here
- Other Research Collaborators: here
Proposal
Langmuir Monolayers of sweet orange essential oil, the phospholipids dipalmitoylphosphatidylcholine (DPPC), dipalmitoylphosphatidylglycerol (DPPG) and E.coli lipid extract were used to investigate the antibacterial properties of essential oils. The Langmuir Monolayer technique is a useful one for the formation of model cell membranes by allowing analysis of the interactions between the molecules and their organization capabilities. Monolayers using the sweet orange oil as a subphase with DPPC added were found to be more fluid, and to be less stable, as indicated by a low surface pressure at low molecular areas when compared to monolayers with ultra pure water as the subphase. Similarly, monolayers using the sweet orange oil as a subphase with the E. coli lipid extract added were found to be more fluid, and to be less stable, as indicated by a low surface pressure at low molecular areas when compared to monolayers with ultra pure water as the subphase. These results were also found with the trials of DPPG. Further investigations will involve using a simpler gram-negative model, such as 1,2-dipalmitoyl-sn-glycero-3-phospho-L-serine (DPPS), since E. coli is a more complex gram-negative model, and observing those results in the orange oil, changing the concentration of the oil in the subphase and using different essential oils as subphases.
Instruments to be used
Langmuir Monolayer Trough
References (2 minimum)
- "Antibiotic / Antimicrobial Resistance." Centers for Disease Control and Prevention. Centers for Disease Control and Prevention, 02 Mar. 2016. Web. 19 Apr. 2016. <http://www.cdc.gov/drugresistance/>.
- Boire NA, Riedel S, Parrish NM (2013) Essential Oils and Future Antibiotics: New Weapons against Emerging ‘Superbugs’? J Anc Dis Prev Rem 1:105
- Nazzaro, Filomena, Florinda Fratianni, Laura De Martino, Raffaele Coppola, and Vincenzo De Feo. "Effect of Essential Oils on Pathogenic Bacteria."Pharmaceuticals 6.12 (2013): 1451-474. Web.
Research pledge
I, Antonetta Axup, have read the Chem/Bioc 430 course syllabus and understand the general structure and expectations of the research program. The above material was prepared after consultation, and in conjunction with my research advisor.