Difference between revisions of "Celestina Awopileda"

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==Personal Information==
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Biochemistry Major '16
  
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== Celestina's Research Page ==
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==Undergraduate Research Activities==
  
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== Proposed Project Summary ==
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[[CA_spring_2016|Spring 2016]]
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'''Identification of Acetaminophen Free Radical Formation Products'''
 
  
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The primary goal of this project is to identify acetaminophen free radical formation products using GC-MS. The commonly used pain killer APAP is very toxic when ingested in high doses and can result in kidney and liver damage. Cytochrome P-450 is found to be one of the two enzymes that catalyze the oxidation of APAP leading to the formation of toxic metabolites and covalent modification of proteins. This project aims to analyze these metabolites. Samples are to be prepared by acetylation of APAP polymers.
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==Presentations==
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Aug 2015: '''SOFIA Presentation'''
  
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Update on Research work
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Title: Attitudes Toward STEM Introductory Courses
  
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The experimental procedure found in a paper on determination of acetaminophen using GC-MS was followed. The GC was programmed at an initial temperature of 70°C for 1min, ramped up to 250°C at a rate of 30°C/min followed by a hold at 250°C for 20 min in splitless mode. The sample was made by acetylation of acetaminophen by adding drops of acetic anhydride and 3uL of pyridine. 1-μL of acetylated acetaminophen in methanol was injected, and there was no peak detection at all for 3 runs.
 
  
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In order to get a working solution, several dilutions of stock unacetylated acetaminophen with methanol were made within the concentration ranges of 75-400ug/mL. Injections of different concentration(100ug/mL, 200ug/mL, 400ug/mL)resulted in no peak detection after 2 runs were conducted for each. Some of the data were saved even though there was no peaks observed. To further the overall research work until a working solution is developed, progress has been made in finding journal articles for writing the abstract and introduction of the paper on this project.
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Nov 2015: Chem 350: '''Science Seminar'''
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Title: ''Gas Chromatography-Mass Spectrum Analysis of Aroma Compounds in Coffee''
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Apr 2016: Chem 350: '''Science Seminar'''
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Title:
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==Interests==
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Dancing
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==Career Plans==

Latest revision as of 06:14, 15 March 2016

Personal Information

Biochemistry Major '16

Undergraduate Research Activities

Spring 2016

Presentations

Aug 2015: SOFIA Presentation

Title: Attitudes Toward STEM Introductory Courses


Nov 2015: Chem 350: Science Seminar

Title: Gas Chromatography-Mass Spectrum Analysis of Aroma Compounds in Coffee


Apr 2016: Chem 350: Science Seminar

Title:

Interests

Dancing

Career Plans

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