Difference between revisions of "Immobilized Enzymes"

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(Created page with "The immobilization procedure can be found here. The procedure has been applied to the following: ==HRP/H2O2/tyrosine/NO== ==Cyt c== Chen et al....")
 
 
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The immobilization procedure can be found here.
 
The immobilization procedure can be found here.
 +
 +
Examples of application [[File:Slide1.png|200px|thumb|left|here]]
  
 
The procedure has been applied to the following:
 
The procedure has been applied to the following:
 +
 +
==HRP/H2O2/TCP==
 +
:'''Revisiting the Peroxidase Oxidation of 2,4,6-Trihalophenols: ESR Detection of Radical Intermediates.'''
 +
:Bradley E. Sturgeon,*, Benjamin J. Battenburg, Blake J. Lyon, and Stefan Franzen.
 +
:''Chemical Research in Toxicology'', '''2011''', ''24'', pp. 1862-1868.
 +
:[[Media:Tx200215r.pdf|here]]
 +
[[File:Sturgeon tcp hrp.png|200px|thumb|center|Sturgeon, 2011]]
  
 
==HRP/H2O2/tyrosine/NO==
 
==HRP/H2O2/tyrosine/NO==
 +
:'''Tyrosine iminoxyl radical formation from tyrosyl radical/nitric oxide and nitrosotyrosine.'''
 +
:Sturgeon BE, Glover RE, Chen YR, Burka LT, and Mason RP.
 +
:''Journal of Biological Chemistry'', '''2001''', ''276(49)'', pp. 45516-45521.
 +
:[[Media:J. Biol. Chem.-2001-Sturgeon-45516-21.pdf|here]]
 +
[[File:TyrosylNO rad HRP.png|200px|thumb|center|Sturgeon, 2001]]
  
 
==Cyt c==
 
==Cyt c==
[[Media:Chen jbc 2002.pdf| Chen et al.]]
+
'''Protein Oxidation of Cytochrome c by Reactive Halogen Species Enhances Its Peroxidase Activity.'''
 +
:Yeong-Renn Chen, Leesa J. Deterding, Bradley E. Sturgeon, Kenneth B. Tomer, and Ronald P. Mason
 +
:''Journal of Biological Chemistry'', '''2002''', ''277(33)'', pp. 29781-29791.
 +
:[[Media:Chen jbc 2002.pdf| here]]
 +
[[File:Chen cytc 2002.png|200px|thumb|center|Chen, 2002]]
  
 +
==HRP/H2O2/Acetaminophen==
  
==this==
+
==HRP/H2O2/Lignin monomers==
 +
[[File:Ligin Monomer radicals.png|200px|thumb|center|unpublished data]]
  
==that==
+
==HRP/LPO/H2O2/NAT==

Latest revision as of 18:57, 25 July 2016

The immobilization procedure can be found here.

Examples of application

here

The procedure has been applied to the following:

HRP/H2O2/TCP

Revisiting the Peroxidase Oxidation of 2,4,6-Trihalophenols: ESR Detection of Radical Intermediates.
Bradley E. Sturgeon,*, Benjamin J. Battenburg, Blake J. Lyon, and Stefan Franzen.
Chemical Research in Toxicology, 2011, 24, pp. 1862-1868.
here
Sturgeon, 2011

HRP/H2O2/tyrosine/NO

Tyrosine iminoxyl radical formation from tyrosyl radical/nitric oxide and nitrosotyrosine.
Sturgeon BE, Glover RE, Chen YR, Burka LT, and Mason RP.
Journal of Biological Chemistry, 2001, 276(49), pp. 45516-45521.
here
Sturgeon, 2001

Cyt c

Protein Oxidation of Cytochrome c by Reactive Halogen Species Enhances Its Peroxidase Activity.

Yeong-Renn Chen, Leesa J. Deterding, Bradley E. Sturgeon, Kenneth B. Tomer, and Ronald P. Mason
Journal of Biological Chemistry, 2002, 277(33), pp. 29781-29791.
here
Chen, 2002

HRP/H2O2/Acetaminophen

HRP/H2O2/Lignin monomers

unpublished data

HRP/LPO/H2O2/NAT