Difference between revisions of "Lignan Degradation"
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Lignans for Life Hydroxymatairesinol (HMR) Lignan was obtained from Amazon.com (Seattle, WA, USA), and pH 5 Phosphate-Citrate Buffer, 1,4-Dioxane, and KMnO<sub>4</sub> and were obtained from Sigma Aldrich (St. Louis, MO, USA). | Lignans for Life Hydroxymatairesinol (HMR) Lignan was obtained from Amazon.com (Seattle, WA, USA), and pH 5 Phosphate-Citrate Buffer, 1,4-Dioxane, and KMnO<sub>4</sub> and were obtained from Sigma Aldrich (St. Louis, MO, USA). | ||
| − | A solution of 10 HMR Lignan | + | A solution of 10 HMR Lignan capsules in 100.0 mL of 1,4-Dioxane solution was prepared. This HMR Lignan/dioxane solution (25.0 mL) was added to 25.0 mL of pH 5 Phosphate-Citrate buffer. |
A solution of 0.0395 g of KMnO<sub>4</sub> and 1000 μL H<sub>2</sub>O was prepared. | A solution of 0.0395 g of KMnO<sub>4</sub> and 1000 μL H<sub>2</sub>O was prepared. | ||
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*5.0 mL of the HMR Lignan/dioxane/buffer solution + 15 mMol KMnO<sub>4</sub> | *5.0 mL of the HMR Lignan/dioxane/buffer solution + 15 mMol KMnO<sub>4</sub> | ||
| − | === | + | ===Results and Discussion=== |
| − | [[File:lignandegradation.png|450px|thumb|center|Figure 1: Offset HPLC Analysis of 5.0 mL of HMR Lignan/dioxane/buffer solution and various amounts of KMnO<sub>4</sub> | + | [[File:lignandegradation.png||450px|thumb|center|Figure 1: Offset HPLC Analysis of 5.0 mL of HMR Lignan/dioxane/buffer solution and various amounts of KMnO<sub>4</sub>. The sample was run through an Agilent 1100 HPLC with a C18 column (length: 4.6 x 100 mm and particle size: 3.5 μm) at 28.0°C with a gradient elution at 0-15 min: 100% 0.1% TFA, 0% acetonitrile, 15-25 min: 0% 0.1% TFA, 100% acetonitrile, 25-30 min: 100% 0.1% TFA, 0% acetonitrile. Trials were run once.]] |
Latest revision as of 14:51, 5 May 2021
Materials and Methods
Lignans for Life Hydroxymatairesinol (HMR) Lignan was obtained from Amazon.com (Seattle, WA, USA), and pH 5 Phosphate-Citrate Buffer, 1,4-Dioxane, and KMnO4 and were obtained from Sigma Aldrich (St. Louis, MO, USA).
A solution of 10 HMR Lignan capsules in 100.0 mL of 1,4-Dioxane solution was prepared. This HMR Lignan/dioxane solution (25.0 mL) was added to 25.0 mL of pH 5 Phosphate-Citrate buffer.
A solution of 0.0395 g of KMnO4 and 1000 μL H2O was prepared.
The following solutions were analyzed on an Agilent 1100 HPLC with a C18 column (4.16 x 100 mm length and 3.5 μm particle size) at absorbance wavelength of 270 nm and temperature of 28°C using an acetonitrile (ACN) and 0.1% TFA gradient for 30 minutes. The first 15 minutes were run at 100% 0.1% TFA, 15-25 minutes at 100% ACN, and 25-30 minutes at 100% 0.1% TFA:
- 5.0 mL of the HMR Lignan/dioxane/buffer solution
- 5.0 mL of the HMR Lignan/dioxane/buffer solution + 5 mMol KMnO4
- 5.0 mL of the HMR Lignan/dioxane/buffer solution + 10 mMol KMnO4
- 5.0 mL of the HMR Lignan/dioxane/buffer solution + 15 mMol KMnO4
Results and Discussion
Figure 1: Offset HPLC Analysis of 5.0 mL of HMR Lignan/dioxane/buffer solution and various amounts of KMnO4. The sample was run through an Agilent 1100 HPLC with a C18 column (length: 4.6 x 100 mm and particle size: 3.5 μm) at 28.0°C with a gradient elution at 0-15 min: 100% 0.1% TFA, 0% acetonitrile, 15-25 min: 0% 0.1% TFA, 100% acetonitrile, 25-30 min: 100% 0.1% TFA, 0% acetonitrile. Trials were run once.