Difference between revisions of "Oxybenzone Oxidation"

Latest revision as of 20:16, 1 April 2020

The oxidation of oxybenzone was attempted with a reaction of oxybenzone, dioxane, and potassium permanganate.

Making the Samples For Chemical Oxidation

2 mM Oxybenzone Buffer Stock Solution

Combined 50 mL of dioxane, 50 mL of pH 7 buffer solution, and 45.7 mg of oxybenzone in a bottle. Added the oxybenzone to the dioxane, and then added the buffer solution.

Potassium Permanganate Stock Solutions

2mM Potassium Permanganate Stock Solution

Dissolved 0.0316 g of potassium permanganate in 100 mL of RO water.

4mM Potassium Permanganate Stock Solution

Dissolved 0.0632 g of potassium permanganate in 100 mL of RO water.

10mM Potassium Permanganate Stock Solution

Dissolved 0.158 g of potassium permanganate in 100 mL of RO water.

20mM Potassium Permanganate Stock Solution

Dissolved 0.316 g of potassium permanganate in 100 mL of RO water.

Preparing the final samples

Added 5 mL of the oxybenzone buffer stock solution to a scintillation vial. Then added 5 mL of the 1 mM Potassium Permanganate Stock Solution to the same vial that contained the oxybenzone buffer solution. Mixed solution thoroughly. This vial now contained 1 mM oxybenzone solution and 1 mM potassium permanganate solution once dilution was accounted for. This process was repeated with the rest of the potassium permanganate solutions.

g KMnO4	Concentration of Stock Solution KMnO4 (mM)	Concentration of KMnO4 After Dilution (mM)	Concentration of Oxybenzone After Dilution (mM)
0.0316	2	1	1
0.0632	4	2	1
0.158	10	5	1
0.316	20	10	1

Data

Figure 1. Graph of oxybenzone dissolved in methanol.

Figure 2. Graph depicting the decrease in oxybenzone as the concentration of potassium permanganate increases.

Figure 3. Representation of the Oxidation of Oxybenzone and Oxidation Products.

@@ Line 1: / Line 1: @@
-The oxidation of oxybenzone was attempted with a reaction of oxybenzone, hydrogen peroxide, and horseradish peroxidase.
+The oxidation of oxybenzone was attempted with a reaction of oxybenzone, dioxane, and potassium permanganate.
+==Making the Samples For Chemical Oxidation==
-==Making the Samples For Enzymatic Oxidation==
-Our actual samples to be tested required the making of a 2 mM oxybenzone pH 5 buffer stock solution, a hydrogen peroxide stock solution, and a horseradish peroxidase solution.
-mM Oxybenzone Buffer Stock Solution
-Combined 50 mL of dioxane, 50 mL of pH 5 buffer solution, and 45.7 mg of oxybenzone in a bottle. Added the oxybenzone to the dioxane, and then added the buffer solution.
-Hydrogen Peroxide Stock Solution
-Measured 5 mL of water into a scintillation vial. Added 283 microliters of 30% hydrogen peroxide to the water. This provided a hydrogen peroxide solution of about 0.413 M.
-Horseradish Peroxidase Stock Solution
+==== 2 mM Oxybenzone Buffer Stock Solution ====
-Preparing the Final Samples
+Combined 50 mL of dioxane, 50 mL of pH 7 buffer solution, and 45.7 mg of oxybenzone in a bottle. Added the oxybenzone to the dioxane, and then added the buffer solution.
-Measured out 5 mL of the 2 mM oxybenzone buffer stock solution into 4 different scintillation vials. Added hydrogen peroxide solution in different amounts to each vial (6 microliters, 12 microliters, 18 microliters, and 24 microliters). Add 10 microliters of horseradish peroxidase solution to each vial.
+==== Potassium Permanganate Stock Solutions ====
-Unfortunately, our enzymatic oxidation was unsuccessful, so our focus turned to chemical oxidation.
+mM Potassium Permanganate Stock Solution
+* Dissolved 0.0316 g of potassium permanganate in 100 mL of RO water.
-==Making the Samples For Chemical Oxidation==
+mM Potassium Permanganate Stock Solution
+* Dissolved 0.0632 g of potassium permanganate in 100 mL of RO water.
-''2 mM Oxybenzone Buffer Stock Solution''
+mM Potassium Permanganate Stock Solution
+* Dissolved 0.158 g of potassium permanganate in 100 mL of RO water.
-Combined 50 mL of dioxane, 50 mL of pH 5 buffer solution, and 45.7 mg of oxybenzone in a bottle. Added the oxybenzone to the dioxane, and then added the buffer solution.
+mM Potassium Permanganate Stock Solution
+* Dissolved 0.316 g of potassium permanganate in 100 mL of RO water.
-''Potassium Permanganate Stock Solution''
+==== Preparing the final samples ====
+Added 5 mL of the oxybenzone buffer stock solution to a scintillation vial. Then added 5 mL of the 1 mM Potassium Permanganate Stock Solution to the same vial that contained the oxybenzone buffer solution. Mixed solution thoroughly. This vial now contained 1 mM oxybenzone solution and 1 mM potassium permanganate solution once dilution was accounted for. This process was repeated with the rest of the potassium permanganate solutions.
-''Preparing the final samples''
-Measured out 5 mL of the 2 mM oxybenzone buffer stock solution into 4 different scintillation vials. Added potassium permanganate solution in increasing concentrations to each vial (6 microliters, 12 microliters, 18 microliters, and 24 microliters).
+{| class="wikitable"
+|-
+! g KMnO4 !! Concentration of Stock Solution KMnO4 (mM) !! Concentration of KMnO4 After Dilution (mM) !! Concentration of Oxybenzone After Dilution (mM)
+|-
+| 0.0316 || 2 || 1 || 1
+|-
+| 0.0632 || 4 || 2 || 1
+|-
+| 0.158|| 10 || 5 || 1
+|-
+| 0.316 || 20 || 10 || 1
+|}
 ==Data==
-[[File:oxyb.png|400px|thumb|left|Figure 1. Graph of oxybenzone dissolved in methanol.]]
+[[File:oxyb.png|500px|thumb|left|Figure 1. Graph of oxybenzone dissolved in methanol.]]
+[[File:oxybenzone_021420_oxidation.jpg|600px|thumb|right|Figure 2. Graph depicting the decrease in oxybenzone as the concentration of potassium permanganate increases.]]
-[[File:oxybh2o2.png|400px|thumb|left|Figure 2. Graph of the samples tested with hydrogen peroxide (6 ul) catalyzed by horseradish peroxidase.]]
+[[File:posterpicture_research.jpg|600px|thumb|left|Figure 3. Representation of the Oxidation of Oxybenzone and Oxidation Products.]]