Difference between revisions of "Caffeine HPLC Method"
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=Results= | =Results= | ||
− | Caffeine should be detected as sharp peaks at 275nm | + | Caffeine should be detected as sharp peaks which elute at approximately 11 minutes when monitoring 275nm on the UV Detector. |
+ | |||
+ | The image below is the spectra of a caffeine standard run with the above method. | ||
[[File:Std.PNG|thumb|250px|frame|left|Caffeine Standard Spectra]] | [[File:Std.PNG|thumb|250px|frame|left|Caffeine Standard Spectra]] |
Revision as of 15:28, 19 April 2016
HPLC method for caffeine analysis
High Pressure Liquid Chromatography Setup (HPLC)
Results
Caffeine should be detected as sharp peaks which elute at approximately 11 minutes when monitoring 275nm on the UV Detector.
The image below is the spectra of a caffeine standard run with the above method.