Difference between revisions of "FT-IR (Nicolet)"
| (9 intermediate revisions by 3 users not shown) | |||
| Line 8: | Line 8: | ||
* Open OMNIC software | * Open OMNIC software | ||
* Clean the instrument with ethanol and a KimWipe | * Clean the instrument with ethanol and a KimWipe | ||
| − | * Collect background data with | + | * Collect background data with sample holder screwed down (Col Bkg) |
| − | * Place sample and screw down the | + | * Place sample and screw down the sample holder |
* Col Sample (Col Sam) | * Col Sample (Col Sam) | ||
* Delete background data by clicking on the background spectrum and hitting the "Clear" Button | * Delete background data by clicking on the background spectrum and hitting the "Clear" Button | ||
| − | * Save sample as a .csv file onto a flash drive | + | * Save sample as a .csv file onto a flash drive to export |
~OR page 30-36 on the user manual~ | ~OR page 30-36 on the user manual~ | ||
| − | === | + | ===Gas samples=== |
* Set up the air tube for the FTIR | * Set up the air tube for the FTIR | ||
* Collect background of the air in the air tube (Col Bkg) | * Collect background of the air in the air tube (Col Bkg) | ||
* Vent out air and allow the air sample to enter the tube | * Vent out air and allow the air sample to enter the tube | ||
* Collect sample (Col Sam) | * Collect sample (Col Sam) | ||
| − | *Delete background data by clicking on the background spectrum and hitting the "Clear" Button | + | * Delete background data by clicking on the background spectrum and hitting the "Clear" Button |
* Save sample as a .csv file onto a flash drive; our instrument doesn't have internet | * Save sample as a .csv file onto a flash drive; our instrument doesn't have internet | ||
| Line 39: | Line 39: | ||
==Other links of interest== | ==Other links of interest== | ||
| + | Fourier transform infrared spectroscopy (FTIR) is a technique which is used to obtain an infrared spectrum of absorption or emission of a solid, liquid or gas. An FTIR spectrometer simultaneously collects high spectral resolution data over a wide spectral range. This confers a significant advantage over a dispersive spectrometer which measures intensity over a narrow range of wavelengths at a time. Rotational–vibrational spectroscopy is a branch of molecular spectroscopy concerned with infrared and Raman spectra of molecules in the gas phase. Transitions involving changes in both vibrational and rotational states can be abbreviated as rovibrational (or ro-vibrational) transitions. | ||
| + | https://en.wikipedia.org/wiki/Infrared_spectroscopy has some helpful gifs of how to tell if a molecule is FTIR active or not. | ||
| + | |||
| + | [[File:FTIR instrument.png|400px|thumb|left|FTIR instrument]] | ||
[[File:FTIR_cheatsheet_2.gif|400px|thumb|left|FTIR_cheatsheet]] | [[File:FTIR_cheatsheet_2.gif|400px|thumb|left|FTIR_cheatsheet]] | ||
Latest revision as of 16:03, 30 September 2016
Model
Thermo Nicolet Nexus 470 FT-IR
User manual : http://mmrc.caltech.edu/FTIR/Nicolet/Nicolet%20Software/Nicolet%202/4700_6700_User.pdf
Operating Instructions
Solid Samples
- Open OMNIC software
- Clean the instrument with ethanol and a KimWipe
- Collect background data with sample holder screwed down (Col Bkg)
- Place sample and screw down the sample holder
- Col Sample (Col Sam)
- Delete background data by clicking on the background spectrum and hitting the "Clear" Button
- Save sample as a .csv file onto a flash drive to export
~OR page 30-36 on the user manual~
Gas samples
- Set up the air tube for the FTIR
- Collect background of the air in the air tube (Col Bkg)
- Vent out air and allow the air sample to enter the tube
- Collect sample (Col Sam)
- Delete background data by clicking on the background spectrum and hitting the "Clear" Button
- Save sample as a .csv file onto a flash drive; our instrument doesn't have internet
Downloading Data Files
- Download the .csv
- Load waves into Igor
- Create a graph
- Right click on the x axis numbers and hit swap to make the spectra look identical to the computer's right after the sample was collected
Example Spectra
Links to manufacturer: http://www.gmi-inc.com/thermo-nicolet-nexus-470-ftir.html
Other links of interest
Fourier transform infrared spectroscopy (FTIR) is a technique which is used to obtain an infrared spectrum of absorption or emission of a solid, liquid or gas. An FTIR spectrometer simultaneously collects high spectral resolution data over a wide spectral range. This confers a significant advantage over a dispersive spectrometer which measures intensity over a narrow range of wavelengths at a time. Rotational–vibrational spectroscopy is a branch of molecular spectroscopy concerned with infrared and Raman spectra of molecules in the gas phase. Transitions involving changes in both vibrational and rotational states can be abbreviated as rovibrational (or ro-vibrational) transitions. https://en.wikipedia.org/wiki/Infrared_spectroscopy has some helpful gifs of how to tell if a molecule is FTIR active or not.
