Difference between revisions of "UV-Vis (HP 8453)"
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1.) Turn on the system and the corresponding computer by pressing the power button on the bottom left corner of the instrument and the power button on the front of the computer tower. | 1.) Turn on the system and the corresponding computer by pressing the power button on the bottom left corner of the instrument and the power button on the front of the computer tower. | ||
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2.) Open UV-visible ChemStation Software. | 2.) Open UV-visible ChemStation Software. | ||
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3.) Collect blank by placing sample solvent in cuvette, placing the cuvette in the sample holder, and clicking the blank option in the software window. Wait until the instrument has finished collecting data. | 3.) Collect blank by placing sample solvent in cuvette, placing the cuvette in the sample holder, and clicking the blank option in the software window. Wait until the instrument has finished collecting data. | ||
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4.) Remove and rinse cuvette. Place sample in the cuvette. Place the cuvette in the sample holder. Click the sample option in the software window. | 4.) Remove and rinse cuvette. Place sample in the cuvette. Place the cuvette in the sample holder. Click the sample option in the software window. | ||
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5.) After the instrument all data has been collected and saved, properly dispose of samples and rinse cuvettes | 5.) After the instrument all data has been collected and saved, properly dispose of samples and rinse cuvettes | ||
Revision as of 20:30, 25 February 2016
Summary
Ultraviolet-visible spectroscopy(UV-Vis) is spectroscopy within the ultraviolet-visible range. UV-vis is used for the quantitative determination of analytes by measuring absorbance. This absorbance can then be used to determine the concentration of a given analyte using Beer's law, which states that the absorbance of a solution is directly proportional to the concentration and the path length.
Our Instrument
Our UV-Vis system is an Agilent 8453. This system uses a combination of a deuterium-discharge lamp for the ultra-violet wavelength range and a tungsten lamp for the visible and near-infrared wavelength range. The system uses a photo diode array. The pathlength used is 1cm.
Using our Instrument
1.) Turn on the system and the corresponding computer by pressing the power button on the bottom left corner of the instrument and the power button on the front of the computer tower.
2.) Open UV-visible ChemStation Software.
3.) Collect blank by placing sample solvent in cuvette, placing the cuvette in the sample holder, and clicking the blank option in the software window. Wait until the instrument has finished collecting data.
4.) Remove and rinse cuvette. Place sample in the cuvette. Place the cuvette in the sample holder. Click the sample option in the software window.
5.) After the instrument all data has been collected and saved, properly dispose of samples and rinse cuvettes
downloading data files
Agilent: http://www.agilent.com/en-us/products/uv-vis-uv-vis-nir
User Manual: http://www.agilent.com/cs/library/usermanuals/public/G1115-90042_OperatorManual.pdf
Spectra of Red, Yellow, and Blue color dye
