Difference between revisions of "UV-Vis (HP 8453)"

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Our UV-Vis system is an Agilent 8453. This system uses a combination of a deuterium-discharge lamp for the ultra-violet wavelength range and a tungsten lamp for the visible and near-infrared wavelength range. The system uses a photo diode array. The pathlength used is 1cm.
 
Our UV-Vis system is an Agilent 8453. This system uses a combination of a deuterium-discharge lamp for the ultra-violet wavelength range and a tungsten lamp for the visible and near-infrared wavelength range. The system uses a photo diode array. The pathlength used is 1cm.
  
operating instructions
+
'''Using our Instrument'''
 +
 
 +
Turn on the system by pressing the
  
 
downloading data files
 
downloading data files

Revision as of 20:16, 25 February 2016

Summary

Ultraviolet-visible spectroscopy(UV-Vis) is spectroscopy within the ultraviolet-visible range. UV-vis is used for the quantitative determination of analytes by measuring absorbance. This absorbance can then be used to determine the concentration of a given analyte using Beer's law, which states that the absorbance of a solution is directly proportional to the concentration and the path length.

Our Instrument

Our UV-Vis system is an Agilent 8453. This system uses a combination of a deuterium-discharge lamp for the ultra-violet wavelength range and a tungsten lamp for the visible and near-infrared wavelength range. The system uses a photo diode array. The pathlength used is 1cm.

Using our Instrument

Turn on the system by pressing the

downloading data files

Spectra of Red, Yellow, and Blue color dye

alt text

Agilent: http://www.agilent.com/en-us/products/uv-vis-uv-vis-nir

User Manual: http://www.agilent.com/cs/library/usermanuals/public/G1115-90042_OperatorManual.pdf