Difference between revisions of "UV-Vis (HP 8453)"

From MC Chem Wiki
Jump to navigation Jump to search
Line 1: Line 1:
 
'''Summary'''
 
'''Summary'''
 +
 
Ultraviolet-visible spectroscopy(UV-Vis) is spectroscopy within the ultraviolet-visible range. UV-vis is used for the quantitative determination of analytes by measuring absorbance. This absorbance can then be used to determine the concentration of a given analyte using Beer's law, which states that the absorbance of a solution is directly proportional to the concentration and the path length.
 
Ultraviolet-visible spectroscopy(UV-Vis) is spectroscopy within the ultraviolet-visible range. UV-vis is used for the quantitative determination of analytes by measuring absorbance. This absorbance can then be used to determine the concentration of a given analyte using Beer's law, which states that the absorbance of a solution is directly proportional to the concentration and the path length.
  
 
'''Our Instrument'''
 
'''Our Instrument'''
Our UV-Vis system is an Agilent 8453. This system uses a combination of a deuterium-discharge lamp.
+
 
 +
Our UV-Vis system is an Agilent 8453. This system uses a combination of a deuterium-discharge lamp for the ultra-violet wavelength range and a tungsten lamp for the visible and near-infrared wavelength range. The system uses a photo diode array. The pathlength used is 1cm.
  
 
operating instructions
 
operating instructions

Revision as of 20:11, 25 February 2016

Summary

Ultraviolet-visible spectroscopy(UV-Vis) is spectroscopy within the ultraviolet-visible range. UV-vis is used for the quantitative determination of analytes by measuring absorbance. This absorbance can then be used to determine the concentration of a given analyte using Beer's law, which states that the absorbance of a solution is directly proportional to the concentration and the path length.

Our Instrument

Our UV-Vis system is an Agilent 8453. This system uses a combination of a deuterium-discharge lamp for the ultra-violet wavelength range and a tungsten lamp for the visible and near-infrared wavelength range. The system uses a photo diode array. The pathlength used is 1cm.

operating instructions

downloading data files

Spectra of Red, Yellow, and Blue color dye Three colors.png

Agilent: http://www.agilent.com/en-us/products/uv-vis-uv-vis-nir

other links of insterest