Difference between revisions of "UV-Vis (HP 8453)"
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Revision as of 20:02, 25 February 2016
Summary Ultraviolet-visible spectroscopy(UV-Vis) is spectroscopy within the ultraviolet-visible range. UV-vis is used for the quantitative determination of analytes by measuring absorbance. This absorbance can then be used to determine the concentration of a given analyte using Beer's law, which states that the absorbance of a solution is directly proportional to the concentration and the path length.
Our Instrument Our UV-Vis system is an Agilent 8453. This system uses a combination of a deuterium-discharge lamp.
operating instructions
downloading data files
example spectra
Agilent: http://www.agilent.com/en-us/products/uv-vis-uv-vis-nir
other links of insterest