Lignan Degradation

Materials and Methods

Lignans for Life Hydroxymatairesinol (HMR) Lignan was obtained from Amazon.com (Seattle, WA, USA), and pH 5 Phosphate-Citrate Buffer, 1,4-Dioxane, and KMnO₄ and were obtained from Sigma Aldrich (St. Louis, MO, USA).

A solution of 10 HMR Lignan capsules in 100.0 mL of 1,4-Dioxane solution was prepared. This HMR Lignan/dioxane solution (25.0 mL) was added to 25.0 mL of pH 5 Phosphate-Citrate buffer.

A solution of 0.0395 g of KMnO₄ and 1000 μL H₂O was prepared.

The following solutions were analyzed on an Agilent 1100 HPLC with a C18 column (4.16 x 100 mm length and 3.5 μm particle size) at absorbance wavelength of 270 nm and temperature of 28°C using an acetonitrile (ACN) and 0.1% TFA gradient for 30 minutes. The first 15 minutes were run at 100% 0.1% TFA, 15-25 minutes at 100% ACN, and 25-30 minutes at 100% 0.1% TFA:

• 5.0 mL of the HMR Lignan/dioxane/buffer solution
• 5.0 mL of the HMR Lignan/dioxane/buffer solution + 5 mMol KMnO₄
• 5.0 mL of the HMR Lignan/dioxane/buffer solution + 10 mMol KMnO₄
• 5.0 mL of the HMR Lignan/dioxane/buffer solution + 15 mMol KMnO₄

Materials and Methods

Figure 1: Offset HPLC Analysis of 5.0 mL of HMR Lignan/dioxane/buffer solution and various amounts of KMnO₄. The sample was run through an Agilent 1100 HPLC with a C18 column (length: 4.6 x 100 mm and particle size: 3.5 μm) at 28.0°C with a gradient elution at 0-15 min: 100% 0.1% TFA, 0% acetonitrile, 15-25 min: 0% 0.1% TFA, 100% acetonitrile, 25-30 min: 100% 0.1% TFA, 0% acetonitrile. Trials were run once.