Difference between revisions of "Cannabis"
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::Farmers need to know the THC concentrations of their cannabis material. Therefore, the FDA will not shut them down. For example, if the THC concentration is above the 0.3 WT% they will have a whole field of marijuana and not hemp. | ::Farmers need to know the THC concentrations of their cannabis material. Therefore, the FDA will not shut them down. For example, if the THC concentration is above the 0.3 WT% they will have a whole field of marijuana and not hemp. | ||
:::https://www.healthline.com/health/hemp-vs-marijuana | :::https://www.healthline.com/health/hemp-vs-marijuana | ||
| − | ===Method Development=== | + | ===Structures of Cannabinoids=== |
| − | + | ==CBD== | |
| + | [[File:CBD_Photo.png|400px]] | ||
| + | ==CBDa== | ||
| + | [[File:CBDa_Photo_Structure.png|400px]] | ||
| + | |||
| + | [[File:CBDa_e_space.png|400px]] | ||
| + | ::Figure 1: CBDa Electrostatic potential | ||
| + | |||
| + | ==THC== | ||
| + | [[File:THC_Photo_Structure.png|400px]] | ||
| + | |||
| + | ==THCa== | ||
| + | [[File:THCa_Photo_Structure.png|400px]] | ||
| + | |||
| + | ==Method Development== | ||
| + | ===Handling of Plant Material=== | ||
:: Before the plant is analyzed there are many steps to insure the best data outcome. This process is important because we do not want impurities in the solution, also we want the highest concentration of the cannabinoids that are present in the plant material. | :: Before the plant is analyzed there are many steps to insure the best data outcome. This process is important because we do not want impurities in the solution, also we want the highest concentration of the cannabinoids that are present in the plant material. | ||
| − | + | ===Method of Analysis=== | |
::: 1) Manicure the plant | ::: 1) Manicure the plant | ||
::: 2) Use the grinder to grind up the sample | ::: 2) Use the grinder to grind up the sample | ||
| Line 60: | Line 75: | ||
::: 10) Run the sample with the standards of the cannabinoids that should be present with the sample | ::: 10) Run the sample with the standards of the cannabinoids that should be present with the sample | ||
::: 11) Collect the data and plot the peaks on Igor | ::: 11) Collect the data and plot the peaks on Igor | ||
| + | ::::https://www.sigmaaldrich.com/technical-documents/articles/analytical/food-beverage/analysis-of-cannabinoids.html | ||
==High Performance Liquid Chromatography Method Development== | ==High Performance Liquid Chromatography Method Development== | ||
| Line 66: | Line 82: | ||
:: This can be done by running the sample through the UV-Vis to see the lambda max. This will show you the highest wavelength that this cannabinoids will elute off the column. | :: This can be done by running the sample through the UV-Vis to see the lambda max. This will show you the highest wavelength that this cannabinoids will elute off the column. | ||
==Data Analysis== | ==Data Analysis== | ||
| − | [[File:THC_CBD_Std.png|400px]] | + | [[File:THC_CBD_Std.png|600px]] |
| + | ::Figure 1: Standards of CBD and THC along with the lambda max of both cannabinoids | ||
| + | |||
| + | |||
| + | [[File:Hemp_Sample_Galena_IL.png|400px]] | ||
| + | ::Figure 2: CBD sample from Galena IL | ||
| + | |||
| + | |||
| + | [[File:Galena_CBD_THC_STD.png|400px]] | ||
| + | ::Figure 3: Galena CBD sample ran with both standards (CBD and THC) | ||
| + | |||
| + | |||
| + | [[File:Soda_CBD.png|400px]] | ||
| + | ::Figure 4: CBD Sparkling water ran with both standards (CBD and THC) | ||
| + | |||
| + | |||
| + | [[File:Sparking_Water_Can.png|400px]] | ||
==Analyical Testing Links== | ==Analyical Testing Links== | ||
Latest revision as of 20:19, 23 April 2021
Chemistry Based Links
Keynote: The Chemistry Behind Cannabinoid Acids
- Professor Raphael Mechoulam
- Hebrew University
- Center on Cannabinoid Research
- 56 min
- Oct 9, 2019
- July 17, 2017
- 3 min
Emerald/Perkin Elmer Webinar
News Links
Lake Superior State University - News Story
- Link to Program
- Nov 2019
Cannabis Research
Importance of Testing Cannabis
- Hemp vs. Marijuana
- If the concentration of the cannabis material is over 0.3 WT% it is considered marijuana. If the concentration of the cabbages material is under 0.3 WT% it is considered hemp.
- Marijuana is used for medicinal uses such as a pain reliever for cancer patiences. Also, many other cannabinoids are used for joint pain and other treatments.
- Hemp's primary function is for using its plant material for thread to make T-Shirts, rope, textiles, and many other uses.
- If the concentration of the cannabis material is over 0.3 WT% it is considered marijuana. If the concentration of the cabbages material is under 0.3 WT% it is considered hemp.
- Growing Hemp Material
- Farmers need to know the THC concentrations of their cannabis material. Therefore, the FDA will not shut them down. For example, if the THC concentration is above the 0.3 WT% they will have a whole field of marijuana and not hemp.
Structures of Cannabinoids
CBD
CBDa
- Figure 1: CBDa Electrostatic potential
THC
THCa
Method Development
Handling of Plant Material
- Before the plant is analyzed there are many steps to insure the best data outcome. This process is important because we do not want impurities in the solution, also we want the highest concentration of the cannabinoids that are present in the plant material.
Method of Analysis
- 1) Manicure the plant
- 2) Use the grinder to grind up the sample
- 3) Empty the grinder into a glass jar
- 4) Weigh out 0.100 g / 100mg on an analytical balance
- 5) Place the 0.100 g of the sample into a falcon tube and add 5ml of Methanol (make sure all of the sample makes it into the tube)
- 6) Put the falcon tube onto the shaker (30min) for the max RPM
- 7) Use a filter syringe to filter out the liquid from the plant material. (Place filter on the syringe after the liquid is taken out of the tube)
- 8) Use the syringe to put the liquid into a HPLC testing capsule
- 9) Place the capsule into the HPLC
- 10) Run the sample with the standards of the cannabinoids that should be present with the sample
- 11) Collect the data and plot the peaks on Igor
High Performance Liquid Chromatography Method Development
- The HPLC detector should be set at 270nm
- The detector needs to be set at the correct wavelength because if it is not set at the wavelength the cannabinoids peaks will not appear.
- This can be done by running the sample through the UV-Vis to see the lambda max. This will show you the highest wavelength that this cannabinoids will elute off the column.
Data Analysis
- Figure 1: Standards of CBD and THC along with the lambda max of both cannabinoids
- Figure 2: CBD sample from Galena IL
- Figure 3: Galena CBD sample ran with both standards (CBD and THC)
- Figure 4: CBD Sparkling water ran with both standards (CBD and THC)
Analyical Testing Links
- Nov 18, 2011
High Tech: Inside a Cannabis Testing Lab
- May 8, 2017