Difference between revisions of "Cannabis"

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:[https://www.lssu.edu/college-science-environment/school-science-medicine/cannabis-chemistry/ Link to Program]
 
:[https://www.lssu.edu/college-science-environment/school-science-medicine/cannabis-chemistry/ Link to Program]
 
:Nov 2019
 
:Nov 2019
 +
 
==Cannabis Research==
 
==Cannabis Research==
=Importance=
+
===Importance of Testing Cannabis===
 +
:Hemp vs. Marijuana
 +
:: If the concentration of the cannabis material is over 0.3 WT% it is considered marijuana. If the concentration of the cabbages material is under 0.3 WT% it is considered hemp.
 +
::: Marijuana is used for medicinal uses such as a pain reliever for cancer patiences. Also, many other cannabinoids are used for joint pain and other treatments.
 +
::: Hemp's primary function is for using its plant material for thread to make T-Shirts, rope, textiles, and many other uses.
 +
:Growing Hemp Material
 +
::Farmers need to know the THC concentrations of their cannabis material. Therefore, the FDA will not shut them down. For example, if the THC concentration is above the 0.3 WT% they will have a whole field of marijuana and not hemp.
 +
:::https://www.healthline.com/health/hemp-vs-marijuana
 +
===Structures of Cannabinoids===
 +
==CBD==
 +
[[File:CBD_Photo.png|400px]]
 +
==CBDa==
 +
[[File:CBDa_Photo_Structure.png|400px]]
 +
 
 +
[[File:CBDa_e_space.png|400px]]
 +
::Figure 1: CBDa Electrostatic potential
 +
 
 +
==THC==
 +
[[File:THC_Photo_Structure.png|400px]]
 +
 
 +
==THCa==
 +
[[File:THCa_Photo_Structure.png|400px]]
 +
 
 +
==Method Development==
 +
===Handling of Plant Material===
 +
:: Before the plant is analyzed there are many steps to insure the best data outcome. This process is important because we do not want impurities in the solution, also we want the highest concentration of the cannabinoids that are present in the plant material.
 +
===Method of Analysis===
 +
::: 1) Manicure the plant
 +
::: 2) Use the grinder to grind up the sample
 +
::: 3) Empty the grinder into a glass jar
 +
::: 4) Weigh out 0.100 g / 100mg on an analytical balance
 +
::: 5) Place the 0.100 g of the sample into a falcon tube and add 5ml of Methanol (make sure all of the sample makes it into the tube)
 +
::: 6) Put the falcon tube onto the shaker (30min) for the max RPM
 +
::: 7) Use a filter syringe to filter out the liquid from the plant material. (Place filter on the syringe after the liquid is taken out of the tube)
 +
::: 8) Use the syringe to put the liquid into a HPLC testing capsule
 +
::: 9) Place the capsule into the HPLC
 +
::: 10)  Run the sample with the standards of the cannabinoids that should be present with the sample
 +
::: 11) Collect the data and plot the peaks on Igor
 +
::::https://www.sigmaaldrich.com/technical-documents/articles/analytical/food-beverage/analysis-of-cannabinoids.html
 +
 
 +
==High Performance Liquid Chromatography Method Development==
 +
:The HPLC detector should be set at 270nm
 +
:: The detector needs to be set at the correct wavelength because if it is not set at the wavelength the cannabinoids peaks will not appear.
 +
:: This can be done by running the sample through the UV-Vis to see the lambda max. This will show you the highest wavelength that this cannabinoids will elute off the column.
 +
==Data Analysis==
 +
[[File:THC_CBD_Std.png|600px]]
 +
::Figure 1: Standards of CBD and THC along with the lambda max of both cannabinoids
 +
 
 +
 
 +
[[File:Hemp_Sample_Galena_IL.png|400px]]
 +
::Figure 2: CBD sample from Galena IL
 +
 
 +
 
 +
[[File:Galena_CBD_THC_STD.png|400px]]
 +
::Figure 3: Galena CBD sample ran with both standards (CBD and THC)
 +
 
 +
 
 +
[[File:Soda_CBD.png|400px]]
 +
::Figure 4: CBD Sparkling water ran with both standards (CBD and THC)
 +
 
 +
 
 +
[[File:Sparking_Water_Can.png|400px]]
  
 
==Analyical Testing Links==
 
==Analyical Testing Links==

Latest revision as of 20:19, 23 April 2021

Chemistry Based Links

Keynote: The Chemistry Behind Cannabinoid Acids

Professor Raphael Mechoulam
Hebrew University
Center on Cannabinoid Research
56 min
Oct 9, 2019

ACS - Cannabis & The Chemist

July 17, 2017
3 min

Emerald/Perkin Elmer Webinar

News Links

Lake Superior State University - News Story

Link to Program
Nov 2019

Cannabis Research

Importance of Testing Cannabis

Hemp vs. Marijuana
If the concentration of the cannabis material is over 0.3 WT% it is considered marijuana. If the concentration of the cabbages material is under 0.3 WT% it is considered hemp.
Marijuana is used for medicinal uses such as a pain reliever for cancer patiences. Also, many other cannabinoids are used for joint pain and other treatments.
Hemp's primary function is for using its plant material for thread to make T-Shirts, rope, textiles, and many other uses.
Growing Hemp Material
Farmers need to know the THC concentrations of their cannabis material. Therefore, the FDA will not shut them down. For example, if the THC concentration is above the 0.3 WT% they will have a whole field of marijuana and not hemp.
https://www.healthline.com/health/hemp-vs-marijuana

Structures of Cannabinoids

CBD

CBD Photo.png

CBDa

CBDa Photo Structure.png

CBDa e space.png

Figure 1: CBDa Electrostatic potential

THC

THC Photo Structure.png

THCa

THCa Photo Structure.png

Method Development

Handling of Plant Material

Before the plant is analyzed there are many steps to insure the best data outcome. This process is important because we do not want impurities in the solution, also we want the highest concentration of the cannabinoids that are present in the plant material.

Method of Analysis

1) Manicure the plant
2) Use the grinder to grind up the sample
3) Empty the grinder into a glass jar
4) Weigh out 0.100 g / 100mg on an analytical balance
5) Place the 0.100 g of the sample into a falcon tube and add 5ml of Methanol (make sure all of the sample makes it into the tube)
6) Put the falcon tube onto the shaker (30min) for the max RPM
7) Use a filter syringe to filter out the liquid from the plant material. (Place filter on the syringe after the liquid is taken out of the tube)
8) Use the syringe to put the liquid into a HPLC testing capsule
9) Place the capsule into the HPLC
10) Run the sample with the standards of the cannabinoids that should be present with the sample
11) Collect the data and plot the peaks on Igor
https://www.sigmaaldrich.com/technical-documents/articles/analytical/food-beverage/analysis-of-cannabinoids.html

High Performance Liquid Chromatography Method Development

The HPLC detector should be set at 270nm
The detector needs to be set at the correct wavelength because if it is not set at the wavelength the cannabinoids peaks will not appear.
This can be done by running the sample through the UV-Vis to see the lambda max. This will show you the highest wavelength that this cannabinoids will elute off the column.

Data Analysis

THC CBD Std.png

Figure 1: Standards of CBD and THC along with the lambda max of both cannabinoids


Hemp Sample Galena IL.png

Figure 2: CBD sample from Galena IL


Galena CBD THC STD.png

Figure 3: Galena CBD sample ran with both standards (CBD and THC)


Soda CBD.png

Figure 4: CBD Sparkling water ran with both standards (CBD and THC)


Sparking Water Can.png

Analyical Testing Links

How Marijuana Is Tested

Nov 18, 2011

High Tech: Inside a Cannabis Testing Lab

May 8, 2017