Free Radical Generation

The 1-electron oxidation of a phenol can be accomplished via chemical or enzymatic means.

Chemical Means

1. KMnO4

KMnO4

To make a 2 mM solution of KMnO4, we added 0.0316g of KMnO4 solid to 100 mL dH2O

To make a 2 mM solution of BHA, we added 0.0304 g of solid HPA to 100 mL dH2O

2. Cerium (IV)

For Cerium Free Radicals: 100 mL solution of 2 mM HPA and 100 mL solution of Ce (IV).

For 100 mL of 2 mM HPA:

Weigh out 30.4mg of HPA

Add it into 100 mL DI water

For 100 mL of 2 mM Ce (IV)

Weigh out 109.6 mg of Ammonium Cerium Nitrate

Add that to 2.5 mL of 18M sulfuric acid

Add that solution to 97.5 mL of DI water

3. Fenton Chemistry

References

Fenton/Phenol (2005)

Fenton/DMPO (2016)

[Media:1-s2.0-S0891584996004327-main.pdf|Mason (1997) Fenton/DMPO]]

Fe2+ + H2O2 ---> OH• + phenol ---> phenoxyl radical

Solutions

1. HPA and hydrogen peroxide (100mL)

2 mmolar HPA with 2 mmolar hydrogen peroxide

 a. HPA made with 0.0300g of 152.015g/mol HPA
 b. 30 microliters of (30%) hydrogen peroxide was added

2. Iron(II) (100mL)

1M phosphoric acid with 1 milligram of Iron (II)

 a. 6.82 mL Phosphoric acid was mixed with 93.18 mL of RO water
 b. 1 mg of FeSO4 was added

ESR Results

These results were inconclusive at this time