Yasaer Y Moore - Revision history

Bes: /* Undergraduate Research Activities */

2022-07-31T19:50:02Z

Undergraduate Research Activities

Bes: /* Undergraduate Research Activities */

2022-07-31T19:49:36Z

Undergraduate Research Activities

Bes: /* Undergraduate Research Activities */

2022-07-31T19:49:14Z

Undergraduate Research Activities

Bes: /* Undergraduate Research Activities */

2022-07-31T19:48:59Z

Undergraduate Research Activities

Bes: /* Undergraduate Research Activities */

2022-07-31T19:48:36Z

Undergraduate Research Activities

Bes: /* Undergraduate Research Activities */

2022-07-11T23:02:30Z

Undergraduate Research Activities

Bes: /* Undergraduate Research Activities */

2022-07-11T22:55:25Z

Undergraduate Research Activities

Bes: /* Undergraduate Research Activities */

2022-07-11T22:47:07Z

Undergraduate Research Activities

Bes: /* Undergraduate Research Activities */

2022-07-11T22:46:25Z

Undergraduate Research Activities

Bes: /* Undergraduate Research Activities */

2022-07-11T22:44:48Z

Undergraduate Research Activities

@@ Line 8: / Line 8: @@
 ==Undergraduate Research Activities==
 [[Exploring the Bacteria Found In Underwater Environments|Exploring the Bacteria Found In Underwater Environments]]
 <!--:- Abstract YM
 ::* All organisms have a metabolic mechanism in which they obtain energy. This mechanism is called an oxidation-reduction (redox) reaction. The specific microorganisms I am interested in are the ones that use the Sulfate-Reduction Chain. They are called SRMs for short (Sulfate-reducing microorganisms); these microorganisms or at other times called microbes or microbiome(s) are found in Blue Holes, located in the Bahamas. The goal of my project is to test different water sources found around the city of Monmouth, isolate the DNA from each source using vacuum filtration, and then run a PCR reaction to examine the concentration level of the bacteria’s DNA.-->
+:- Abstract <!--BS-->
 ::* It is estimated that there is 550 x 10<sup>15</sup> grams of carbon on the earth; this is also known as 550 Gt C (gigatonnes carbon). Plants make up 450 Gt C (82%), while animals make up only 2 Gt C (0.4%). Humans make up only 0.06 Gt C (0.01%). The second largest contributor to Earth's biomass is bacteria at 70 Gt C (13%) and ironically we know the least about the role bacteria play in our daily lives. In 2016, the US Federal Government launched the National Microbiome Initiative. This initiative acknowledged the presence of bacteria/microbial communities ''"liv[ing] on or in people, plants, soil, oceans, lakes, rocks, and the atmosphere"'' and that understanding more about these bacteria/microbial communities would advance our knowledge in areas such as ''"health care, food production, and environmental restoration."'' During our project this summer we learned how to isolate bacteria from a variety of substrates with a particular interest in isolating bacterial DNA from water samples. Bacterial DNA was isolated, quantified using fluorescence (Quibit) and sent off to the University of Illinois Biotech Center for 16s - V4 Illumina amplification/sequencing. The sequencing results were then analyzed using Qiime2 software to generate taxonomy bar plots showing bacterial species information and quantity.

@@ Line 9: / Line 9: @@
 [[Exploring the Bacteria Found In Underwater Environments|Exploring the Bacteria Found In Underwater Environments]]
-<--:- Abstract YM
+<!--:- Abstract YM
-::* All organisms have a metabolic mechanism in which they obtain energy. This mechanism is called an oxidation-reduction (redox) reaction. The specific microorganisms I am interested in are the ones that use the Sulfate-Reduction Chain. They are called SRMs for short (Sulfate-reducing microorganisms); these microorganisms or at other times called microbes or microbiome(s) are found in Blue Holes, located in the Bahamas. The goal of my project is to test different water sources found around the city of Monmouth, isolate the DNA from each source using vacuum filtration, and then run a PCR reaction to examine the concentration level of the bacteria’s DNA.--!>
+::* All organisms have a metabolic mechanism in which they obtain energy. This mechanism is called an oxidation-reduction (redox) reaction. The specific microorganisms I am interested in are the ones that use the Sulfate-Reduction Chain. They are called SRMs for short (Sulfate-reducing microorganisms); these microorganisms or at other times called microbes or microbiome(s) are found in Blue Holes, located in the Bahamas. The goal of my project is to test different water sources found around the city of Monmouth, isolate the DNA from each source using vacuum filtration, and then run a PCR reaction to examine the concentration level of the bacteria’s DNA.-->
 :- Abstract BS

@@ Line 9: / Line 9: @@
 [[Exploring the Bacteria Found In Underwater Environments|Exploring the Bacteria Found In Underwater Environments]]
-<!--:- Abstract YM
+<--:- Abstract YM
 ::* All organisms have a metabolic mechanism in which they obtain energy. This mechanism is called an oxidation-reduction (redox) reaction. The specific microorganisms I am interested in are the ones that use the Sulfate-Reduction Chain. They are called SRMs for short (Sulfate-reducing microorganisms); these microorganisms or at other times called microbes or microbiome(s) are found in Blue Holes, located in the Bahamas. The goal of my project is to test different water sources found around the city of Monmouth, isolate the DNA from each source using vacuum filtration, and then run a PCR reaction to examine the concentration level of the bacteria’s DNA.--!>

@@ Line 9: / Line 9: @@
 [[Exploring the Bacteria Found In Underwater Environments|Exploring the Bacteria Found In Underwater Environments]]
--->:- Abstract YM
+<!--:- Abstract YM
 ::* All organisms have a metabolic mechanism in which they obtain energy. This mechanism is called an oxidation-reduction (redox) reaction. The specific microorganisms I am interested in are the ones that use the Sulfate-Reduction Chain. They are called SRMs for short (Sulfate-reducing microorganisms); these microorganisms or at other times called microbes or microbiome(s) are found in Blue Holes, located in the Bahamas. The goal of my project is to test different water sources found around the city of Monmouth, isolate the DNA from each source using vacuum filtration, and then run a PCR reaction to examine the concentration level of the bacteria’s DNA.--!>

@@ Line 9: / Line 9: @@
 [[Exploring the Bacteria Found In Underwater Environments|Exploring the Bacteria Found In Underwater Environments]]
-:- Abstract YM
+-->:- Abstract YM
-::* All organisms have a metabolic mechanism in which they obtain energy. This mechanism is called an oxidation-reduction (redox) reaction. The specific microorganisms I am interested in are the ones that use the Sulfate-Reduction Chain. They are called SRMs for short (Sulfate-reducing microorganisms); these microorganisms or at other times called microbes or microbiome(s) are found in Blue Holes, located in the Bahamas. The goal of my project is to test different water sources found around the city of Monmouth, isolate the DNA from each source using vacuum filtration, and then run a PCR reaction to examine the concentration level of the bacteria’s DNA.
+::* All organisms have a metabolic mechanism in which they obtain energy. This mechanism is called an oxidation-reduction (redox) reaction. The specific microorganisms I am interested in are the ones that use the Sulfate-Reduction Chain. They are called SRMs for short (Sulfate-reducing microorganisms); these microorganisms or at other times called microbes or microbiome(s) are found in Blue Holes, located in the Bahamas. The goal of my project is to test different water sources found around the city of Monmouth, isolate the DNA from each source using vacuum filtration, and then run a PCR reaction to examine the concentration level of the bacteria’s DNA.--!>
 :- Abstract BS

@@ Line 13: / Line 13: @@
 :- Abstract BS
-::* It is estimated that there is 550 x 10<sup>15</sup> grams of carbon on the earth; this is also known as 550 Gt C (gigatonnes carbon). Plants make up 450 Gt C (82%), while animals make up only 2 Gt C (0.4%). Humans make up only 0.06 Gt C (0.01%). The second largest contributor to Earth's biomass is bacteria at 70 Gt C (13%) and ironically we know the least about the role bacteria play in our daily lives. In 2016, the US Federal Government launched the National Microbiome Initiative. This initiative acknowledged the presence of bacteria/microbial communities ''"liv[ing] on or in people, plants, soil, oceans, lakes, rocks, and the atmosphere"'' and that understanding more about these bacteria/microbial communities would advance our knowledge in areas such as ''"health care, food production, and environmental restoration."'' During our project this summer we learned how to isolate bacteria from a variety of substrates with a particular interest in isolating bacterial DNA from water samples. Bacterial DNA was isolated, quantified using fluorescence (Quibit) and sent off to the University of Illinois Biotech Center for Illumina sequencing.
+::* It is estimated that there is 550 x 10<sup>15</sup> grams of carbon on the earth; this is also known as 550 Gt C (gigatonnes carbon). Plants make up 450 Gt C (82%), while animals make up only 2 Gt C (0.4%). Humans make up only 0.06 Gt C (0.01%). The second largest contributor to Earth's biomass is bacteria at 70 Gt C (13%) and ironically we know the least about the role bacteria play in our daily lives. In 2016, the US Federal Government launched the National Microbiome Initiative. This initiative acknowledged the presence of bacteria/microbial communities ''"liv[ing] on or in people, plants, soil, oceans, lakes, rocks, and the atmosphere"'' and that understanding more about these bacteria/microbial communities would advance our knowledge in areas such as ''"health care, food production, and environmental restoration."'' During our project this summer we learned how to isolate bacteria from a variety of substrates with a particular interest in isolating bacterial DNA from water samples. Bacterial DNA was isolated, quantified using fluorescence (Quibit) and sent off to the University of Illinois Biotech Center for 16s - V4 Illumina amplification/sequencing. The sequencing results were then analyzed using Qiime2 software to generate taxonomy bar plots showing bacterial species information and quantity.
 [[File:bacteria biomass.PNG|thumb|none]]