UV-Vis (HP 8453) - Revision history

Sdistin at 16:09, 30 September 2016

2016-09-30T16:09:59Z

2016-02-25T20:33:57Z

2016-02-25T20:33:36Z

2016-02-25T20:32:00Z

2016-02-25T20:30:44Z

2016-02-25T20:30:08Z

2016-02-25T20:17:17Z

2016-02-25T20:17:01Z

2016-02-25T20:16:31Z

2016-02-25T20:16:22Z

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 '''Our Instrument:'''
-Our UV-Vis system is an Agilent 8453. This system uses a combination of a deuterium-discharge lamp for the ultra-violet wavelength range and a tungsten lamp for the visible and near-infrared wavelength range. The system uses a photo diode array. The pathlength used is 1cm.
+Our UV-Vis system is an Agilent 8453. This system uses a combination of a deuterium-discharge lamp for the ultra-violet wavelength range and a tungsten lamp for the visible and near-infrared wavelength range. The system uses a photo diode array.
 '''Using our Instrument:'''

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-'''Summary'''
+'''Summary:'''
 Ultraviolet-visible spectroscopy(UV-Vis) is spectroscopy within the ultraviolet-visible range. UV-vis is used for the quantitative determination of analytes by measuring absorbance. This absorbance can then be used to determine the concentration of a given analyte using Beer's law, which states that the absorbance of a solution is directly proportional to the concentration and the path length.
-'''Our Instrument'''
+'''Our Instrument:'''
 Our UV-Vis system is an Agilent 8453. This system uses a combination of a deuterium-discharge lamp for the ultra-violet wavelength range and a tungsten lamp for the visible and near-infrared wavelength range. The system uses a photo diode array. The pathlength used is 1cm.
-'''Using our Instrument'''
+'''Using our Instrument:'''
 .) Turn on the system and the corresponding computer by pressing the power button on the bottom left corner of the instrument and the power button on the front of the computer tower.

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 .) After the instrument all data has been collected and saved, properly dispose of samples and rinse cuvettes
-Downloading data files:
+'''Downloading data files:'''
 After collecting desires data from the instrument, insert thumb drive into a USB port.

@@ Line 19: / Line 19: @@
 .) After the instrument all data has been collected and saved, properly dispose of samples and rinse cuvettes
-downloading data files
+Downloading data files:
 Agilent: http://www.agilent.com/en-us/products/uv-vis-uv-vis-nir

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 .) Turn on the system and the corresponding computer by pressing the power button on the bottom left corner of the instrument and the power button on the front of the computer tower.
 .) Open UV-visible ChemStation Software.
 .) Collect blank by placing sample solvent in cuvette, placing the cuvette in the sample holder, and clicking the blank option in the software window. Wait until the instrument has finished collecting data.
 .) Remove and rinse cuvette. Place sample in the cuvette. Place the cuvette in the sample holder. Click the sample option in the software window.
 .) After the instrument all data has been collected and saved, properly dispose of samples and rinse cuvettes

← Older revision		Revision as of 20:17, 25 February 2016
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	downloading data files		downloading data files

−	Spectra of Red, Yellow, and Blue color dye	+	Spectra of Red, Yellow, and Blue color dye [[File:three colors.png\|200px\|thumb\|left\|alt text]]
−	[[File:three colors.png\|200px\|thumb\|]

← Older revision		Revision as of 20:16, 25 February 2016
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	Spectra of Red, Yellow, and Blue color dye		Spectra of Red, Yellow, and Blue color dye
−	[[File:three colors.png\|200px\|thumb\|~~left\|alt text]~~]	+	[[File:three colors.png\|200px\|thumb\|]

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 Spectra of Red, Yellow, and Blue color dye
 [[File:three colors.png|200px|thumb|left|alt text]]
 Agilent: http://www.agilent.com/en-us/products/uv-vis-uv-vis-nir
 User Manual: http://www.agilent.com/cs/library/usermanuals/public/G1115-90042_OperatorManual.pdf