Tbailey at 15:40, 28 September 2016

2016-09-28T15:40:11Z

Tbailey: Created page with "Chemistry/Biochemistry Research 430 :Fall 2016 :Brad Dulee :Senior Biochemistry Major ==Research Times== TBD - 4 hours per week : section 01 = 0.25 credit = 4 hours per week...."

2016-09-28T15:39:55Z

Created page with "Chemistry/Biochemistry Research 430 :Fall 2016 :Brad Dulee :Senior Biochemistry Major ==Research Times== TBD - 4 hours per week : section 01 = 0.25 credit = 4 hours per week...."

New page

Chemistry/Biochemistry Research 430
:Fall 2016
:Brad Dulee
:Senior Biochemistry Major

==Research Times==
TBD - 4 hours per week
: section 01 = 0.25 credit = 4 hours per week.

==Proposed Research Project==
===Increasing Growth-Inhibitory Compound Tolerance in ''Escherichia coli'' For Use in Biofuel Production===

===General Information===
:Advisor: Laura Moore
:Other research student collaborators: Brad Dulee

===Proposal===
It is the goal of this project to focus on increasing the tolerance of ''Escherichia coli'' against the harmful byproducts of lignocellulosic biomass pretreatment methods for use in biofuel production.
===Instruments to be used===
:- I2400 incubator shaker (37°C)
:- Allegra 25R Centrifuge (4°C)
:- Bio Rad MicroPulser Electroporator
:- Multiwell FC Microwell Plate Reader
:- Nanodrop

===References (2 minimum)===
1. Mills, T. Y.; Sandoval, N. R.; Gill, R. T. Cellulosic hydrolysate toxicity and tolerance mechanisms in "Escherichia coli". "Biotechnology for Biofuels" 2009, 2:26.

2. Olsson, L.; Hahn-Hägerdal, B.; Fermentation of Lignocellulosic Hydrosylates for Ethanol Production. "Enzyme and Microbial Technology". 1996, 18, 312-331.

3. Richardson,T. L.; Harner, N. K.; Bajwa, P. K.; Trevors, J. T.; Lee, H. Approaches to Deal with Toxic Inhibitors During Fermentation of Lignocellulosic Substrates. "ACS Symposium Series". 2011, 1067, Chapter 7, 171-202.

4. Wang, X.; Miller, E. N.; Yomano, L. P.; Zhang, X.; Shanmugam, K. T.; Ingram, L. O. Increased Furfural Tolerance Due to Overexpression of NADH-Dependent Oxidoreductase FucO in "Escherichia coli" Strains Engineered for the Production of Ethanol and Lactate. "Applied and Environment Biology" 2011, 77:15, 5132-5140.

5. Wang, X.; Miller, E. N.; Yomano, L. P.; Zhang, X.; Shanmugam, K. T.; Ingram, L. O. Increased Furan Tolerance in "Escherichia Coli" Due to a Cryptic ucpA Gene. "Applied and Environmental Microbiology". 2012, 78:7, 2452-2455.

6. Weil, J. R.; Dien, B.; Bothast, R.; Hendrickson, R.; Mosier, N. S.; Ladisch, M. R. Removal of Fermentation Inhibitors Formed during Pretreatment of Biomass by Polymeric Adsorbents. "Ind. Eng. Chem." Res. 2002, 41:24, 6132-6138.

===Research pledge===
I, Tyler bailey, have read the Chem/Bioc 430 course syllabus and understand the general structure and expectations of the research program. The above material was prepared after consultation, and in conjunction with my research advisor.

@@ Line 1: / Line 1: @@
 Chemistry/Biochemistry Research 430
 :Fall 2016
-:Brad Dulee
+:Tyler Bailey
 :Senior Biochemistry Major

Tyler Bailey Chem430 F16 - Revision history

Tbailey at 15:40, 28 September 2016

Tbailey: Created page with "Chemistry/Biochemistry Research 430 :Fall 2016 :Brad Dulee :Senior Biochemistry Major ==Research Times== TBD - 4 hours per week : section 01 = 0.25 credit = 4 hours per week...."