Synthesis and Characterization of Lignin Monomers - Revision history

Rsitko750: /* Experimental Procedures */

2020-05-14T23:00:55Z

Experimental Procedures

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Experimental Procedures

2020-05-14T22:53:38Z

Results

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Results

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Experimental Procedures

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Experimental Procedures

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Materials

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Materials

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Conclusion

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 * '''Dimerization:''' Product of ethyl ferulate dissolved in pH 4.0 acetate buffer. Solution is cooled and added to peroxidase(10 mg in 2 mL of phosphate buffer). Product filtered out to obtain crude product.
 * '''Purification:''' Use of flash chromatography with EtOAc as eluant. Product recrystallized allowing for NMR data and melting point.
 *'''Esterification:''' 0.36 M ferulic acid in ethanol (500 mL) with concentrated HCl. Refluxed for two days. Cooled and ethanol evaporated under pressure (rotovap). Crude extract combined with ethyl acetate (250 mL). Solution dried over anhydrous MgSO<sub>4</sub>.

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 * '''Purification:''' Use of flash chromatography with EtOAc as eluant. Product recrystallized allowing for NMR data and melting point.
+Preparation_________
 *'''Esterification:''' 0.36 M ferulic acid in ethanol (500 mL) with concentrated HCl. Refluxed for two days. Cooled and ethanol evaporated under pressure (rotovap). Crude extract combined with ethyl acetate (250 mL). Solution dried over anhydrous MgSO<sub>4</sub>.

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 =Results=
-[[File:HPLC Data.jpg|400px|right]]
+While minimal research was able to be accomplished, discussion on methods were able to be looked at to finding a more green chemistry approach. The initial method allowed for a two day reflux accumulating in a significant amount of water being used (roughly 700+ gallons). Producing the ferulic acid standard is used in HPLC analysis. Running samples of standards and product from experimental procedures through the HPLC instrument looking at the intensity over a retention time of 30 minutes.
-While minimal research was able to be accomplished, discussion on methods were able to be looked at to finding a more green chemistry approach. The initial method allowed for a two day reflux accumulating in a significant amount of water being used (roughly 700+ gallons). Producing the ferulic acid standard is used in HPLC analysis. Running samples of standards and product from experimental procedures through the HPLC instrument looking at the intensity over a retention time f 30 minutes.
+[[File:HPLC Data.jpg|400px]] [[File:Screenshot (2).png|400px]]
 =Discusion=

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 [[File:HPLC Data.jpg|400px|right]]
-While minimal research was able to be accomplished, discussion on methods were able to be looked at to finding a more green chemistry approach. The initial method allowed for a two day reflux accumulating in a significant amount of water being used (roughly 700+ gallons). Producing the ferulic acid standard is used in HPLC analysis. Running samples of standards and product through
+While minimal research was able to be accomplished, discussion on methods were able to be looked at to finding a more green chemistry approach. The initial method allowed for a two day reflux accumulating in a significant amount of water being used (roughly 700+ gallons). Producing the ferulic acid standard is used in HPLC analysis. Running samples of standards and product from experimental procedures through the HPLC instrument looking at the intensity over a retention time f 30 minutes.
 [[File:Screenshot (2).png|400px|right]]

← Older revision		Revision as of 22:34, 14 May 2020
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	[[File:HPLC Data.jpg\|400px\|right]]		[[File:HPLC Data.jpg\|400px\|right]]

−	While minimal research was able to be accomplished, discussion on methods were able to be looked at to finding a more green chemistry approach. The initial method allowed for a two day reflux accumulating in a significant amount of water being used (roughly 700+ gallons).	+	While minimal research was able to be accomplished, discussion on methods were able to be looked at to finding a more green chemistry approach. The initial method allowed for a two day reflux accumulating in a significant amount of water being used (roughly 700+ gallons). Producing the ferulic acid standard is used in HPLC analysis. Running samples of standards and product through

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 Preparation of 2 mM Ferulic Acid Standards
+* 100 mL solution is made with pH 5 buffer using deionized water
-mL solution is made with pH 5 buffer using deionized water
+* 0.0385 g of Ferulic Acid added to pH 5 buffer
 Preparation through 8--5 Coupled Diferulate Procedure ()

← Older revision		Revision as of 22:32, 14 May 2020
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	==Experimental Procedures==		==Experimental Procedures==
		+
		+	Preparation of 2 mM Ferulic Acid Standards
		+
		+	100 mL solution is made with pH 5 buffer using deionized water
		+
		+	0.0385 g of Ferulic Acid added to pH 5 buffer

	Preparation through 8--5 Coupled Diferulate Procedure ()		Preparation through 8--5 Coupled Diferulate Procedure ()

← Older revision		Revision as of 22:29, 14 May 2020
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	[https://www.sigmaaldrich.com/catalog/product/aldrich/d7927?lang=en&region=US Sinapic acid]		[https://www.sigmaaldrich.com/catalog/product/aldrich/d7927?lang=en&region=US Sinapic acid]
		+
		+	Ethanol
		+
		+	Acetyl Chloride

	===Safety===		===Safety===

← Older revision		Revision as of 22:27, 14 May 2020
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	[https://www.sigmaaldrich.com/catalog/product/aldrich/d7927?lang=en&region=US Sinapic acid]		[https://www.sigmaaldrich.com/catalog/product/aldrich/d7927?lang=en&region=US Sinapic acid]
		+
		+	===Safety===

	==Experimental Procedures==		==Experimental Procedures==

← Older revision		Revision as of 22:27, 14 May 2020
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	=Conclusion=		=Conclusion=
		+
		+	Through more compassion between procedures would allow for a more green approach in a laboratory setting. With the possibility of a microwave reactor would allow for lignin synthesis to be rapidly produced.

	== References ==		== References ==