SAHounsve Spring 2018 - Revision history

Shounsve: /* Results and Discussion */

2018-05-11T00:08:44Z

Results and Discussion

Shounsve: /* Experimental */

2018-05-11T00:05:27Z

Experimental

Shounsve: /* Experimental */

2018-05-10T23:56:32Z

Experimental

Shounsve: /* References */

2018-05-10T23:51:21Z

References

Shounsve: /* Conclusion */

2018-05-10T23:51:03Z

Conclusion

Shounsve: /* Discussion */

2018-05-10T23:50:54Z

Discussion

Shounsve: /* Results and Discussion */

2018-05-10T23:50:47Z

Results and Discussion

Shounsve: /* Results */

2018-05-10T23:48:49Z

Results

Shounsve: /* Results */

2018-05-10T23:48:32Z

Results

Shounsve: /* Experimental */

2018-05-10T23:46:55Z

Experimental

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 ===Results and Discussion===
-[[File:eug_trails_only_a.png|400px|thumb|left|Figure 2: Chromatograph of 1 mM eugenol standard and oxidation mixtures. The eugenol standard is present at 13 minutes, at 9 minutes there is a small peak that is unknown at what is present here. At 16 minutes, there is a peak that could be a possible radical product.]]
+[[File:eug_trails_only_a.png|400px|thumb|left|Figure 2: HPLC chromatograph of 1 mM eugenol standard and oxidation mixtures. The eugenol standard is present at 13 minutes, at 9 minutes there is a small peak that is unknown at what is present here. At 16 minutes, there is a peak that could be a possible radical product.]]
 Eugenol oxidized by HRP activated by hydrogen peroxide in solution is seen in Figure 2.
 The eugenol peak is at 13 minutes, with the top being the 1 mM eugenol standard only. The following peaks below the eugenol standard are increasing amounts of hydrogen peroxide. As the concentration of hydrogen peroxide increases, the eugenol peak decreases. This means that eugenol is being oxidized.
 As eugenol is being oxidized, the peak at 16 minutes could be a possible radical product because this peak increases in height as eugenol decreases.
 There is another peaks at 9 minutes, however we do not know what this peak is at this time.There is a peak at 16 minutes that could be a possible radical product. As the concentration of H<sub>2</sub>O<sub>2</sub> increases, the eugenol peak decreases and the possible radical product at 16 minutes increases, indicating that this could a possible radical product.
 To further investigate if this is a radical product from eugenol is to implement Flash Chromatography and Electron Paramagnetic Resonance (EPR). By utilizing Flash Chromatography, the sample can be injected in the instrument where the mixture is separated into fractions. The specific fractions that contained either the peak at 9 minutes or 16 minutes can be used on either Gas Chromatography –Mass Spectrometry (GC-MS) or Nuclear Magnetic Resonance (NMR) to determine the structure of the radical product. Once the structure of the product is determined, we need to know how this product is related to eugenol. To do so, we would use EPR to determine if it is a free radical and where the unpaired electron is on the product’s structure.

← Older revision		Revision as of 00:05, 11 May 2018
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	Different proportions of solvents such as 0.1% TFA as eluent B and acetonitrile as eluent A were used for the separation. The multiple gradient used for the chromatographic separation consisted of different proportions of eluent A/B (0:100 for 1-15 mins, 80:20 for 15-25 min, and 0:100 for 25-30 min). The mobile phase flow rte was 1.0 mL/min, the sample injection volume 10 μL, and the chromatograph was monitored at 280 nm.		Different proportions of solvents such as 0.1% TFA as eluent B and acetonitrile as eluent A were used for the separation. The multiple gradient used for the chromatographic separation consisted of different proportions of eluent A/B (0:100 for 1-15 mins, 80:20 for 15-25 min, and 0:100 for 25-30 min). The mobile phase flow rte was 1.0 mL/min, the sample injection volume 10 μL, and the chromatograph was monitored at 280 nm.
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−

	Oxidation reaction:		Oxidation reaction:

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 Eugenol standard for HPLC:
 .5 ul of eugenol in 0.1 mM PBS with 10% ethanol, pH 7.4 and 1% tween. Used method "eugenol_SH_020918_b".
 Oxidation reaction:

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 ===References===
 ===Research pledge===
 I, Selene Hounsve, have read the Chem/Bioc 430 course syllabus and understand the general structure and expectations of the research program. The above material was prepared after consultation, and in conjunction with my research advisor.

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 To further investigate if this is a radical product from eugenol is to implement Flash Chromatography and Electron Paramagnetic Resonance (EPR). By utilizing Flash Chromatography, the sample can be injected in the instrument where the mixture is separated into fractions. The specific fractions that contained either the peak at 9 minutes or 16 minutes can be used on either Gas Chromatography –Mass Spectrometry (GC-MS) or Nuclear Magnetic Resonance (NMR) to determine the structure of the radical product. Once the structure of the product is determined, we need to know how this product is related to eugenol. To do so, we would use EPR to determine if it is a free radical and where the unpaired electron is on the product’s structure.
 ===References===
 ===Research pledge===
 I, Selene Hounsve, have read the Chem/Bioc 430 course syllabus and understand the general structure and expectations of the research program. The above material was prepared after consultation, and in conjunction with my research advisor.

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 | 4 || 5μL || 0μL || 10μL
 |}
 ===Results===