SAHounsve Fall 2017 - Revision history

Shounsve: /* Results */

2017-09-26T19:18:08Z

Results

Shounsve: /* Results */

2017-09-26T19:17:02Z

Results

Shounsve: /* Results */

2017-09-26T19:15:58Z

Results

Shounsve: /* Results */

2017-09-26T19:13:39Z

Results

Shounsve: /* Results */

2017-09-26T19:12:59Z

Results

Shounsve: /* Conclusion */

2017-09-19T20:27:27Z

Conclusion

Shounsve: /* Discussion */

2017-09-19T20:27:05Z

Discussion

Shounsve at 20:24, 19 September 2017

2017-09-19T20:24:57Z

Shounsve at 20:21, 19 September 2017

2017-09-19T20:21:15Z

Shounsve at 20:20, 19 September 2017

2017-09-19T20:20:38Z

@@ Line 32: / Line 32: @@
 ===Results===
 Pictures of chromatographs and mass spectrums are shown.
-[[File:b_car_gcms_1.png|400px|thumb|left|Figure 1: B-caryophyllene standard]] [[File:[[File:[[File:eu_gcms_1.png|400px|thumb|left|Figure 2: Eugenol standard]] [[File:lim_gcms_1.png|400px|thumb|left|Figure 3: Limonene standard]][[File:lin_gcms_1.png|400px|thumb|left|Figure 4: Linalool standard]][[File:[[File:[[File:meug_gcms_1.png|400px|thumb|left|Figure 5: Methyl eugenol standard]]
+[[File:b_car_gcms_1.png|400px|thumb|left|Figure 1: B-caryophyllene standard]] [[File:eu_gcms_1.png|400px|thumb|left|Figure 2: Eugenol standard]] [[File:lim_gcms_1.png|400px|thumb|left|Figure 3: Limonene standard]][[File:lin_gcms_1.png|400px|thumb|left|Figure 4: Linalool standard]] [[File:meug_gcms_1.png|400px|thumb|left|Figure 5: Methyl eugenol standard]]
 [[File:cin_fresh_bag_1.png|400px|thumb|left|Figure 6: Sniffed cinnamon basil]][[File:gen_fresh_bag_1.png|400px|thumb|left|Figure 7: Sniffed genovese basil]][[File:lemon_fresh_bag_1.png|400px|thumb|left|Figure 8: Sniffed lemon basil]][[File:lime_fresh_bag_1.png|400px|thumb|left|Figure 9: Sniffed lime basil]][[File:opal_fresh_bag_1.png|400px|thumb|left|Figure 10: Sniffed opal basil]]

@@ Line 32: / Line 32: @@
 ===Results===
 Pictures of chromatographs and mass spectrums are shown.
-[[File:b_car_gcms_1.png|400px|thumb|left|Figure 1: B-caryophyllene standard]] [[File:[[File:eug_gcms_1.png|400px|thumb|left|Figure 2: Eugenol standard]] [[File:lim_gcms_1.png|400px|thumb|left|Figure 3: Limonene standard]][[File:lin_gcms_1.png|400px|thumb|left|Figure 4: Linalool standard]][[File:[[File:meu_gcms_1.png|400px|thumb|left|Figure 5: Methyl eugenol standard]]
+[[File:b_car_gcms_1.png|400px|thumb|left|Figure 1: B-caryophyllene standard]] [[File:[[File:[[File:eu_gcms_1.png|400px|thumb|left|Figure 2: Eugenol standard]] [[File:lim_gcms_1.png|400px|thumb|left|Figure 3: Limonene standard]][[File:lin_gcms_1.png|400px|thumb|left|Figure 4: Linalool standard]][[File:[[File:[[File:meug_gcms_1.png|400px|thumb|left|Figure 5: Methyl eugenol standard]]
 [[File:cin_fresh_bag_1.png|400px|thumb|left|Figure 6: Sniffed cinnamon basil]][[File:gen_fresh_bag_1.png|400px|thumb|left|Figure 7: Sniffed genovese basil]][[File:lemon_fresh_bag_1.png|400px|thumb|left|Figure 8: Sniffed lemon basil]][[File:lime_fresh_bag_1.png|400px|thumb|left|Figure 9: Sniffed lime basil]][[File:opal_fresh_bag_1.png|400px|thumb|left|Figure 10: Sniffed opal basil]]

@@ Line 32: / Line 32: @@
 ===Results===
 Pictures of chromatographs and mass spectrums are shown.
-[[File:b_car_gcms_1.png|400px|thumb|left|Figure 1: B-caryophyllene standard]] [[File:|400px|thumb|left|Figure 2: Eugenol standard]] [[File:lim_gcms_1.png|400px|thumb|left|Figure 3: Limonene standard]][[File:lin_gcms_1.png|400px|thumb|left|Figure 4: Linalool standard]][[File:|400px|thumb|left|Figure 5: Methyl eugenol standard]]
+[[File:b_car_gcms_1.png|400px|thumb|left|Figure 1: B-caryophyllene standard]] [[File:[[File:eug_gcms_1.png|400px|thumb|left|Figure 2: Eugenol standard]] [[File:lim_gcms_1.png|400px|thumb|left|Figure 3: Limonene standard]][[File:lin_gcms_1.png|400px|thumb|left|Figure 4: Linalool standard]][[File:[[File:meu_gcms_1.png|400px|thumb|left|Figure 5: Methyl eugenol standard]]
 [[File:cin_fresh_bag_1.png|400px|thumb|left|Figure 6: Sniffed cinnamon basil]][[File:gen_fresh_bag_1.png|400px|thumb|left|Figure 7: Sniffed genovese basil]][[File:lemon_fresh_bag_1.png|400px|thumb|left|Figure 8: Sniffed lemon basil]][[File:lime_fresh_bag_1.png|400px|thumb|left|Figure 9: Sniffed lime basil]][[File:opal_fresh_bag_1.png|400px|thumb|left|Figure 10: Sniffed opal basil]]

@@ Line 32: / Line 32: @@
 ===Results===
 Pictures of chromatographs and mass spectrums are shown.
-[[File:b_car_gcms_1.png|400px|thumb|left|Figure 1: B-caryophyllene standard]] [[File:eug_gcms_1.png|400px|thumb|left|Figure 2: Eugenol standard]] [[File:lim_gcms_1.png|400px|thumb|left|Figure 3: Limonene standard]][[File:lin_gcms_1.png|400px|thumb|left|Figure 4: Linalool standard]][[File:meu_gcms_1.png|400px|thumb|left|Figure 5: Methyl eugenol standard]]
+[[File:b_car_gcms_1.png|400px|thumb|left|Figure 1: B-caryophyllene standard]] [[File:|400px|thumb|left|Figure 2: Eugenol standard]] [[File:lim_gcms_1.png|400px|thumb|left|Figure 3: Limonene standard]][[File:lin_gcms_1.png|400px|thumb|left|Figure 4: Linalool standard]][[File:|400px|thumb|left|Figure 5: Methyl eugenol standard]]
 [[File:cin_fresh_bag_1.png|400px|thumb|left|Figure 6: Sniffed cinnamon basil]][[File:gen_fresh_bag_1.png|400px|thumb|left|Figure 7: Sniffed genovese basil]][[File:lemon_fresh_bag_1.png|400px|thumb|left|Figure 8: Sniffed lemon basil]][[File:lime_fresh_bag_1.png|400px|thumb|left|Figure 9: Sniffed lime basil]][[File:opal_fresh_bag_1.png|400px|thumb|left|Figure 10: Sniffed opal basil]]

@@ Line 32: / Line 32: @@
 ===Results===
 Pictures of chromatographs and mass spectrums are shown.
-[[File:bcar2.png|400px|thumb|left|Figure 1: B-caryophyllene standard]] [[File:eug2.png|400px|thumb|left|Figure 2: Eugenol standard]] [[File:lim2.png|400px|thumb|left|Figure 3: Limonene standard]][[File:lin2.png|400px|thumb|left|Figure 4: Linalool standard]][[File:meug2.png|400px|thumb|left|Figure 5: Methyl eugenol standard]]
+[[File:b_car_gcms_1.png|400px|thumb|left|Figure 1: B-caryophyllene standard]] [[File:eug_gcms_1.png|400px|thumb|left|Figure 2: Eugenol standard]] [[File:lim_gcms_1.png|400px|thumb|left|Figure 3: Limonene standard]][[File:lin_gcms_1.png|400px|thumb|left|Figure 4: Linalool standard]][[File:meu_gcms_1.png|400px|thumb|left|Figure 5: Methyl eugenol standard]]
 [[File:cin_fresh_bag_1.png|400px|thumb|left|Figure 6: Sniffed cinnamon basil]][[File:gen_fresh_bag_1.png|400px|thumb|left|Figure 7: Sniffed genovese basil]][[File:lemon_fresh_bag_1.png|400px|thumb|left|Figure 8: Sniffed lemon basil]][[File:lime_fresh_bag_1.png|400px|thumb|left|Figure 9: Sniffed lime basil]][[File:opal_fresh_bag_1.png|400px|thumb|left|Figure 10: Sniffed opal basil]]
 ===Discussion===
 The sniffer data has yet to be interpreted until standards are sniffed by GC-MS.

@@ Line 38: / Line 38: @@
 ===Conclusion===
 ===References===
 . Husnu Can Baser, K, and Gerhard Buchbauer. Handbook of Essential Oils: Science, Technology, and Applications. 2nd ed., CRC Press, 2016.

@@ Line 3: / Line 3: @@
 <P ALIGN="left"> Selene Hounsve </P>
 <P ALIGN="left"> Senior Biochemistry Major </P>
-== Research Times ==
+==Research Times==
 Mon/Wed: 8-10 AM
 Tue: 2-5 PM
-== Proposed Research Project  ==
+==Proposed Research Project==
 Extraction, characterization, and application of essential oils from various basil. The characterization of the oils will be done using GC-MS.
 ===Project Title===
@@ Line 14: / Line 14: @@
 ===General Information===
 Advisor: Dr. Bradley E. Sturgeon
-=== Proposal ===
+===Proposal===
 Essential oils are synthesized via secondary metabolic pathways and have a role in plant defenses against pathogens and herbivores, in plant reproduction, attraction of pollinators, and in thermotolerance.  Essential oils can be classified into two different groups based on their metabolic synthesis: terpenoids, which are monoterpenes and sesquiterpenes; and low molecular weight phenylpropanoids.
@@ Line 20: / Line 20: @@
 The goal of this research is to extract the oils of cultivated varieties of basil and make a chemotype table. The analysis will be done via gas chromatography and will employ standards of these chemicals as a tool for identifying compounds. The basis for the expected chemical makeup will be based on Tucker and Thomas’ The Big Book of Herbs: A Comprehensive Illustrated Reference to Herbs of Flavor and Fragrance.
-=== Instruments to be used ===
+===Instruments to be used===
 Steam Distillation, GC-MS, Sniffer
-=== Experimental ===
+===Experimental===
 GC-MS standards were created by 2uL of compound in 2mL methylene chloride. Compounds were eugenol, methyl eugenol, B-caryophyllene, S-limonene, and linalool.
@@ Line 30: / Line 30: @@
 Currently, using sniffer to take in gas from standards and basil for chromatograph and mass spectrum.
-=== Results ===
+===Results===
 Pictures of chromatographs and mass spectrums are shown.
 [[File:bcar2.png|400px|thumb|left|Figure 1: B-caryophyllene standard]] [[File:eug2.png|400px|thumb|left|Figure 2: Eugenol standard]] [[File:lim2.png|400px|thumb|left|Figure 3: Limonene standard]][[File:lin2.png|400px|thumb|left|Figure 4: Linalool standard]][[File:meug2.png|400px|thumb|left|Figure 5: Methyl eugenol standard]]
 [[File:cin_fresh_bag_1.png|400px|thumb|left|Figure 6: Sniffed cinnamon basil]][[File:gen_fresh_bag_1.png|400px|thumb|left|Figure 7: Sniffed genovese basil]][[File:lemon_fresh_bag_1.png|400px|thumb|left|Figure 8: Sniffed lemon basil]][[File:lime_fresh_bag_1.png|400px|thumb|left|Figure 9: Sniffed lime basil]][[File:opal_fresh_bag_1.png|400px|thumb|left|Figure 10: Sniffed opal basil]]
-=== Discussion ===
+===Discussion===
-=== Conclusion ===
+===Conclusion===
-=== References ===
+===References===
 . Husnu Can Baser, K, and Gerhard Buchbauer. Handbook of Essential Oils: Science, Technology, and Applications. 2nd ed., CRC Press, 2016.
@@ Line 47: / Line 47: @@
 . Viña, Amparo, and Elizabeth Murillo. "Essential Oil Composition from Twelve Varieties of Basil (Ocimum spp) Grown in Colombia." Sociedade Brasileira de Química 14.5 (2003): 744-49. Journal Brazilian Chemical Society. Web. 18 Apr. 2017.
-=== Research pledge ===
+===Research pledge===
 I, Selene Hounsve, have read the Chem/Bioc 430 course syllabus and understand the general structure and expectations of the research program. The above material was prepared after consultation, and in conjunction with my research advisor.

@@ Line 1: / Line 1: @@
 <P ALIGN="left"> Chemistry/Biochemistry Research 430 </P>
-:<P ALIGN="left"> Fall 2017 </P>
+<P ALIGN="left"> Fall 2017 </P>
-:<P ALIGN="left"> Selene Hounsve </P>
+<P ALIGN="left"> Selene Hounsve </P>
-:<P ALIGN="left"> Senior Biochemistry Major </P>
+<P ALIGN="left"> Senior Biochemistry Major </P>
 == Research Times ==
 Mon/Wed: 8-10 AM