Riley E Bigham - Revision history

Rileybigham: /* Presentations */

2022-09-05T19:50:21Z

Presentations

2022-09-05T19:45:54Z

2021-09-16T18:08:35Z

Shedd Aquarium

2021-09-16T17:41:20Z

[|Shedd Aquarium

2021-09-16T17:40:11Z

2021-09-16T17:35:06Z

Shedd Aquarium

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Summer 2021

2021-09-15T21:20:07Z

Richard "Doc" Kieft Summer Research

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Summer 2021

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Summer 2021

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 =Presentations=
 *June 2021: '''Richard "Doc" Kieft Summer Research Program'''
 :Title: ''Understanding the Aquatic Microbiome'' (Oral Presentation)

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 Class of 2023
-Junior Biochemistry Major
+Senior Biochemistry Major
 Hometown: O'Fallon, IL

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 ===[https://www.sheddaquarium.org/care-and-conservation/shedd-research/shedd-aquarium-microbiome-project| Shedd Aquarium]===
-A few Doc Kieft research students and our advisor, Bradley Sturgeon had the opportunity to take a behind the scenes tour of the Shedd Aquarium microbiome lab. There we met Frank Oliaro, the lab manager of the microbiome project.
+A few Doc Kieft research students and our advisor, Bradley Sturgeon had the opportunity to take a behind the scenes tour of the Shedd Aquarium microbiome lab. There we met Frank Oliaro, the lab manager of the microbiome project. Here we learned the processes of DNA isolation, PCR, and sequencing. Oiaro also recommended the Earth Microbiome Project page to review the 16s Illumina Amplicon protocol for future PCR and sequencing methods.
 =Presentations=

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 ::''The microbiome is a unique set of microbes living in a particular environment. The microbes that make up the human microbiome are established at birth and evolve throughout life. These microbes are essential for our well-being by regulating our immune system, synthesizing vitamins, and protecting us against certain disease-causing bacteria. The demand for new research has risen in popularity in the past 20 years allowing new discoveries connecting the microbes to the human condition. An example is the use of fecal transplants for treating obesity. New developments have allowed us to understand cause and effects from the rich community of microbes within different environments. As with the human microbiome, aquatic systems also contain a unique collection of microbes. The goal of this project is to evaluate the microbes present in aquatic systems and to correlate the presences of these microbes to the overall health of the aquatic ecosystems.''
-===[|[https://www.sheddaquarium.org/care-and-conservation/shedd-research/shedd-aquarium-microbiome-project| Shedd Aquarium]===
+===[https://www.sheddaquarium.org/care-and-conservation/shedd-research/shedd-aquarium-microbiome-project| Shedd Aquarium]===
 A few Doc Kieft research students and our advisor, Bradley Sturgeon had the opportunity to take a behind the scenes tour of the Shedd Aquarium microbiome lab. There we met Frank Oliaro, the lab manager of the microbiome project.

@@ Line 26: / Line 26: @@
 ::''The microbiome is a unique set of microbes living in a particular environment. The microbes that make up the human microbiome are established at birth and evolve throughout life. These microbes are essential for our well-being by regulating our immune system, synthesizing vitamins, and protecting us against certain disease-causing bacteria. The demand for new research has risen in popularity in the past 20 years allowing new discoveries connecting the microbes to the human condition. An example is the use of fecal transplants for treating obesity. New developments have allowed us to understand cause and effects from the rich community of microbes within different environments. As with the human microbiome, aquatic systems also contain a unique collection of microbes. The goal of this project is to evaluate the microbes present in aquatic systems and to correlate the presences of these microbes to the overall health of the aquatic ecosystems.''
-===Shedd Aquarium===
+===[|[https://www.sheddaquarium.org/care-and-conservation/shedd-research/shedd-aquarium-microbiome-project| Shedd Aquarium]===
 A few Doc Kieft research students and our advisor, Bradley Sturgeon had the opportunity to take a behind the scenes tour of the Shedd Aquarium microbiome lab. There we met Frank Oliaro, the lab manager of the microbiome project.

← Older revision		Revision as of 17:35, 16 September 2021
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	===Shedd Aquarium===		===Shedd Aquarium===
		+
		+	A few Doc Kieft research students and our advisor, Bradley Sturgeon had the opportunity to take a behind the scenes tour of the Shedd Aquarium microbiome lab. There we met Frank Oliaro, the lab manager of the microbiome project.

	=Presentations=		=Presentations=

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 ===Summer 2021===
 *Developed methods for future microbiome research
-*[[Methods_include_Qiagen_DNA_isolation_kits|Methods include Qiagen DNA isolation kits]] (Powerwater, Fecal, and Soil), [[PCR, Sequencing|PCR, Sequencing]]
+*[[Methods_include_Qiagen_DNA_isolation_kits|Methods include Qiagen DNA isolation kits]] (Powerwater, Fecal, and Soil), [[PCR & Sequencing|PCR & Sequencing]]
 *Worked with Dr. Laura Moore and her research members
 *'''Future Research''': The inhabitants of an aquatic microbiome and their effect on a healthy aquatic ecosystem