Research - Revision history

Justin2.1.0: /* Method */

2021-05-12T03:53:43Z

Method

Justin2.1.0: /* Discussion */

2021-05-12T03:51:04Z

Discussion

Justin2.1.0: /* Discussion */

2021-05-12T03:50:15Z

Discussion

Justin2.1.0: /* Results */

2021-05-12T03:35:27Z

Results

Justin2.1.0: /* Results */

2021-05-12T03:34:49Z

Results

Justin2.1.0: /* Results */

2021-05-12T03:28:03Z

Results

Justin2.1.0: /* Results */

2021-05-12T03:27:27Z

Results

Justin2.1.0: /* Results */

2021-05-12T03:26:59Z

Results

Justin2.1.0: /* Results */

2021-05-12T03:25:43Z

Results

Justin2.1.0: /* Method */

2021-05-09T06:16:14Z

Method

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 ==Method==
-Indigo Carmine (IC) powder obtained from Sigma Aldrich was measured with an analytical balance. The measured powder of IC was mixed with RO water with a stir bar to obtain a stock solution, put in a vial, and then calculated and recorded the concentration. The stock solution was diluted to obtain the first using dilution calculations to bring the concentration of the first dilution to half of the stock solution. An amount of the first dilution was taken using a pipette and put in another vial with the corresponding amount of water added to make the concentration ½ of the concentration of what the first dilution was. This process was repeated with every new dilution made until there were five dilutions and each dilution being half the concentration of what the previous dilution was. Each solution (stock solution and five dilutions from stock solution) were measured and wavelengths with corresponding absorbance recorded on the UV-Vis spectrophotometer. These same solutions were also taken to the HPLC to have their absorbance measured and then recorded. Using the absorbance measured and calculated concentrations recorded from the UV-Vis spectrophotometer, a graph of concentration vs absorbance was made and then by finding the slope of the curve was able to find the molar absorptivity of IC.
+Indigo Carmine (IC) powder obtained from Sigma Aldrich was measured with an analytical balance. The measured powder of IC was mixed with RO water with a stir bar to obtain a stock solution, put in a vial, and then calculated and recorded the concentration. The stock solution was diluted to obtain the first using dilution calculations to bring the concentration of the first dilution to half of the stock solution. An amount of the first dilution was taken using a pipette and put in another vial with the corresponding amount of water added to make the concentration ½ of the concentration of what the first dilution was. This process was repeated with every new dilution made until there were five dilutions and each dilution being half the concentration of what the previous dilution was. Each solution (stock solution and five dilutions from stock solution) were measured and wavelengths with corresponding absorbance recorded on the UV-Vis spectrophotometer. These same solutions were also taken to the HPLC to have their absorbance measured and then recorded. Using the absorbance measured and calculated concentrations recorded from the UV-Vis spectrophotometer, a graph of concentration vs absorbance was made and then by finding the slope of the curve, the molar absorptivity of IC was found.
 [[File:ICsol.png|400px]]

← Older revision		Revision as of 03:51, 12 May 2021
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	==Discussion==		==Discussion==

−	The UV-Vis results show that it did not have the capability to measure the absorbance of the compound IC beyond 1.6. As the concentration went up the ~~cure~~ was less smooth	+	The UV-Vis results show that it did not have the capability to measure the absorbance of the compound IC beyond 1.6. As the concentration went up the curve was less smooth.

← Older revision		Revision as of 03:50, 12 May 2021
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	==Discussion==		==Discussion==
		+
		+	The UV-Vis results show that it did not have the capability to measure the absorbance of the compound IC beyond 1.6. As the concentration went up the cure was less smooth

@@ Line 36: / Line 36: @@
 [[File:UVABSVSCONCA.jpg|400px]]
-Figure 3.2: Concentration vs absorbance graph of IC concentrations used to calculate molar absoptivity
+'''Figure 3.2:''' Concentration vs absorbance graph of IC concentrations used to calculate molar absoptivity
 [[File:HPLC CONC ABS.png|400px]]
-Figure 3.4: HPLC graph of all concentrations of IC prepared
+'''Figure 3.4:''' HPLC graph of all concentrations of IC prepared
 ==Discussion==

@@ Line 27: / Line 27: @@
 [[File:Graph4.jpg|400px]]
 [[File:Graph3.jpg|400px]]
 [[File:UVABSVSCONCA.jpg|400px]]
 [[File:HPLC CONC ABS.png|400px]]
 ==Discussion==

← Older revision		Revision as of 03:28, 12 May 2021
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	[[File:UVABSVSCONCA.jpg\|400px]]		[[File:UVABSVSCONCA.jpg\|400px]]
		+
		+	[[File:HPLC CONC ABS.png\|400px]]

	==Discussion==		==Discussion==

← Older revision		Revision as of 03:27, 12 May 2021
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	[[File:Graph3.jpg\|400px]]		[[File:Graph3.jpg\|400px]]
		+
		+	[[File:UVABSVSCONCA.jpg\|400px]]

	==Discussion==		==Discussion==

← Older revision		Revision as of 03:26, 12 May 2021
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	[[File:Graph4.jpg\|400px]]		[[File:Graph4.jpg\|400px]]
		+
		+	[[File:Graph3.jpg\|400px]]

	==Discussion==		==Discussion==

← Older revision		Revision as of 03:25, 12 May 2021
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	==Results==		==Results==
		+
		+	[[File:Graph4.jpg\|400px]]

	==Discussion==		==Discussion==

@@ Line 22: / Line 22: @@
 [[File:ICsol.png|400px]]
-'''Figure 2:''' Indigo Carmine solutions from Stock to dilution 5
+'''Figure 2:''' Indigo Carmine solutions prepared from stock solution to dilution 5
 ==Results==
 ==Discussion==