PCR & Sequencing - Revision history

Rileybigham at 17:28, 16 September 2021

2021-09-16T17:28:28Z

Rileybigham: /* How does sequencing work */

2021-09-16T17:27:46Z

How does sequencing work

Rileybigham: /* How does sequencing work */

2021-09-16T17:27:22Z

How does sequencing work

Rileybigham at 17:26, 16 September 2021

2021-09-16T17:26:11Z

Rileybigham at 17:05, 16 September 2021

2021-09-16T17:05:09Z

Rileybigham: /* How does PCR work? */

2021-09-16T16:43:12Z

How does PCR work?

Rileybigham: /* How does PCR work? */

2021-09-16T16:41:34Z

How does PCR work?

Rileybigham: /* How does PCR work? */

2021-09-16T16:41:20Z

How does PCR work?

Rileybigham at 16:40, 16 September 2021

2021-09-16T16:40:31Z

Rileybigham: Created page with "'''Polymerase chain reaction (PCR)''' A commonly used method to make millions of genetic copies of specific DNA samples. This allows scientists to amplify small samples of DNA..."

2021-09-16T16:11:24Z

Created page with "'''Polymerase chain reaction (PCR)''' A commonly used method to make millions of genetic copies of specific DNA samples. This allows scientists to amplify small samples of DNA..."

New page

'''Polymerase chain reaction (PCR)'''
A commonly used method to make millions of genetic copies of specific DNA samples. This allows scientists to amplify small samples of DNA and make them large enough to analyze.

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 '''Polymerase chain reaction (PCR)'''
 A commonly used method to make millions of genetic copies of specific DNA samples. This allows scientists to amplify small samples of DNA and make them large enough to analyze.
 '''DNA Sequencing'''
 A process used to determine the sequence of nucleotides in a DNA sample. There are two types of sequencing, Sanger Sequencing and Next-generation Sequencing. Sequencing breaks the genome of a DNA into smaller segments, sequencing those segments and assembling the sequences into one long strand.

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 ==How does sequencing work==
 '''Sanger Sequencing'''
-Fragments of DNA are aligned by their overlapping portions  to assemble the sequences of larger DNA regions and later, entire chromosomes. This process is still popularly used today to sequence individual DNA pieces used for cloning or through PCR.[[File:Sanger Sequencing.png|thumbnail|Sanger Sequencing]]
 '''Next-generation sequencing'''
 Next-gen sequencing is like running multiple small sanger sequencing reactions at the same time. This allows sequencing to be faster and cheaper than Sanger.

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 ==How does sequencing work==
 '''Sanger Sequencing'''
-Fragments of DNA are aligned by their overlapping portions  to assemble the sequences of larger DNA regions and later, entire chromosomes. This process is still popularly used today to sequence individual DNA pieces used for cloning or through PCR.
+Fragments of DNA are aligned by their overlapping portions  to assemble the sequences of larger DNA regions and later, entire chromosomes. This process is still popularly used today to sequence individual DNA pieces used for cloning or through PCR.[[File:Sanger Sequencing.png|thumbnail|Sanger Sequencing]]
 '''Next-generation sequencing'''
 Next-gen sequencing is like running multiple small sanger sequencing reactions at the same time. This allows sequencing to be faster and cheaper than Sanger.

@@ Line 3: / Line 3: @@
 '''DNA Sequencing'''
-A process used to determine the sequence of nucleotides in a DNA sample. There are two types of sequencing, '''Sanger Sequencing''' and '''Next-generation Sequencing'''. Sequencing breaks the genome of a DNA into smaller segments, sequencing those segments and assembling the sequences into one long strand.
+A process used to determine the sequence of nucleotides in a DNA sample. There are two types of sequencing, Sanger Sequencing and Next-generation Sequencing. Sequencing breaks the genome of a DNA into smaller segments, sequencing those segments and assembling the sequences into one long strand.
 Sanger sequencing copies the target DNA and make fragments of different lengths. Fluorescent nucleotides mark the ends of the fragments and allow the sequence to be determined.
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 The PCR process uses an automated thermocycler that is programmed to alter the temperature of the reaction every few minutes to allow for DNA separation and synthesis.

@@ Line 1: / Line 1: @@
 '''Polymerase chain reaction (PCR)'''
 A commonly used method to make millions of genetic copies of specific DNA samples. This allows scientists to amplify small samples of DNA and make them large enough to analyze.
 ==How does PCR work?==
 To amplify the sample of DNA, the sample is first heated so the DNA separates into two single stranded DNA. An enzyme called Taq polymerase builds two new strands of DNA. The two original strands of DNA act as templates for the enzyme. Each of the strands are then used to continually create two new copies. The separation and synthesizing process repeats as many as 30 times. This makes over one billion copies of the original DNA segments.
 The PCR process uses an automated thermocycler that is programmed to alter the temperature of the reaction every few minutes to allow for DNA separation and synthesis.

← Older revision		Revision as of 16:40, 16 September 2021
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	'''Polymerase chain reaction (PCR)'''		'''Polymerase chain reaction (PCR)'''
	A commonly used method to make millions of genetic copies of specific DNA samples. This allows scientists to amplify small samples of DNA and make them large enough to analyze.		A commonly used method to make millions of genetic copies of specific DNA samples. This allows scientists to amplify small samples of DNA and make them large enough to analyze.
		+	==How does PCR work?==
		+	To amplify the sample of DNA, the sample is first heated so
		+	the DNA separates into two single stranded DNA. An enzyme called Taq polymerase builds two new strands of DNA. The two original strands of DNA act as templates for the enzyme. Each of the strands are then used to continually create two new copies. The separation and synthesizing process repeats as many as 30 times. This makes over one billion copies of the original DNA segments.
		+	The PCR process uses an automated thermocycler that is programmed to alter the temperature of the reaction every few minutes to allow for DNA separation and synthesis.