Oxybenzone Oxidation - Revision history

Jwelker: /* Preparing the final samples */

2020-04-01T20:16:55Z

Preparing the final samples

Jwelker: /* Potassium Permanganate Stock Solutions */

2020-04-01T20:16:41Z

Potassium Permanganate Stock Solutions

Jwelker: /* 2 mM Oxybenzone Buffer Stock Solution */

2020-04-01T20:16:29Z

2 mM Oxybenzone Buffer Stock Solution

Jwelker: /* Making the Samples For Chemical Oxidation */

2020-04-01T20:16:15Z

Making the Samples For Chemical Oxidation

Jwelker at 20:14, 1 April 2020

2020-04-01T20:14:29Z

Jwelker: /* Data */

2020-04-01T20:13:03Z

Data

Jwelker: /* Data */

2020-04-01T20:12:46Z

Data

Jwelker: /* Data */

2020-04-01T20:12:33Z

Data

Jwelker: /* Data */

2020-04-01T20:12:10Z

Data

Jwelker: /* Data */

2020-04-01T20:11:29Z

Data

@@ Line 20: / Line 20: @@
 * Dissolved 0.316 g of potassium permanganate in 100 mL of RO water.
-==== ''Preparing the final samples'' ====
+==== Preparing the final samples ====
 Added 5 mL of the oxybenzone buffer stock solution to a scintillation vial. Then added 5 mL of the 1 mM Potassium Permanganate Stock Solution to the same vial that contained the oxybenzone buffer solution. Mixed solution thoroughly. This vial now contained 1 mM oxybenzone solution and 1 mM potassium permanganate solution once dilution was accounted for. This process was repeated with the rest of the potassium permanganate solutions.

@@ Line 6: / Line 6: @@
 Combined 50 mL of dioxane, 50 mL of pH 7 buffer solution, and 45.7 mg of oxybenzone in a bottle. Added the oxybenzone to the dioxane, and then added the buffer solution.
-==== ''Potassium Permanganate Stock Solutions'' ====
+==== Potassium Permanganate Stock Solutions ====
 mM Potassium Permanganate Stock Solution
@@ Line 19: / Line 19: @@
 mM Potassium Permanganate Stock Solution
 * Dissolved 0.316 g of potassium permanganate in 100 mL of RO water.
 ==== ''Preparing the final samples'' ====

@@ Line 2: / Line 2: @@
 ==Making the Samples For Chemical Oxidation==
-==== ''2 mM Oxybenzone Buffer Stock Solution'' ====
+==== 2 mM Oxybenzone Buffer Stock Solution ====
 Combined 50 mL of dioxane, 50 mL of pH 7 buffer solution, and 45.7 mg of oxybenzone in a bottle. Added the oxybenzone to the dioxane, and then added the buffer solution.

@@ Line 2: / Line 2: @@
 ==Making the Samples For Chemical Oxidation==
-''2 mM Oxybenzone Buffer Stock Solution''
+==== ''2 mM Oxybenzone Buffer Stock Solution'' ====
 Combined 50 mL of dioxane, 50 mL of pH 7 buffer solution, and 45.7 mg of oxybenzone in a bottle. Added the oxybenzone to the dioxane, and then added the buffer solution.
-''Potassium Permanganate Stock Solutions''
+==== ''Potassium Permanganate Stock Solutions'' ====
 mM Potassium Permanganate Stock Solution
@@ Line 21: / Line 21: @@
-''Preparing the final samples''
+==== ''Preparing the final samples'' ====
 Added 5 mL of the oxybenzone buffer stock solution to a scintillation vial. Then added 5 mL of the 1 mM Potassium Permanganate Stock Solution to the same vial that contained the oxybenzone buffer solution. Mixed solution thoroughly. This vial now contained 1 mM oxybenzone solution and 1 mM potassium permanganate solution once dilution was accounted for. This process was repeated with the rest of the potassium permanganate solutions.
-−
 {| class="wikitable"
 |-

← Older revision		Revision as of 20:14, 1 April 2020
Line 1:		Line 1:
−	The oxidation of oxybenzone was attempted with a reaction of oxybenzone, ~~hydrogen peroxide, and horseradish peroxidase.~~	+	The oxidation of oxybenzone was attempted with a reaction of oxybenzone, dioxane, and potassium permanganate.
−
−	~~==Making the Samples For Enzymatic Oxidation==~~
−
−	~~Our actual samples to be tested required the making of a 2 mM oxybenzone pH 5 buffer stock solution, a hydrogen peroxide stock solution, and a horseradish peroxidase solution.~~
−
−	~~''2 mM Oxybenzone Buffer Stock Solution''~~
−
−	~~Combined 50 mL of~~ dioxane~~, 50 mL of pH 5 buffer solution~~, and ~~45.7 mg of oxybenzone in a bottle. Added the oxybenzone to the dioxane, and then added the buffer solution.~~
−
−	~~''Hydrogen Peroxide Stock Solution''~~
−
−	~~Measured 5 mL of water into a scintillation vial. Added 283 microliters of 30% hydrogen peroxide to the water. This provided a hydrogen peroxide solution of about 0.413 M.~~
−
−	~~''Horseradish Peroxidase Stock Solution''~~
−
−	~~''Preparing the Final Samples''~~
−
−	Measured out 5 mL of the 2 mM oxybenzone buffer stock solution into 4 different scintillation vials. Added hydrogen peroxide solution in different amounts to each vial (6 microliters, 12 microliters, 18 microliters, and 24 microliters). Add 10 microliters of horseradish peroxidase solution to each vial.
−
−	~~Unfortunately, our enzymatic oxidation was unsuccessful, so our focus turned to chemical oxidation~~.
−
	==Making the Samples For Chemical Oxidation==		==Making the Samples For Chemical Oxidation==

@@ Line 61: / Line 61: @@
 ==Data==
-[[File:oxyb.png|400px|thumb|left|Figure 1. Graph of oxybenzone dissolved in methanol.]]
+[[File:oxyb.png|500px|thumb|left|Figure 1. Graph of oxybenzone dissolved in methanol.]]
 [[File:oxybenzone_021420_oxidation.jpg|600px|thumb|right|Figure 2. Graph depicting the decrease in oxybenzone as the concentration of potassium permanganate increases.]]
 [[File:posterpicture_research.jpg|600px|thumb|left|Figure 3. Representation of the Oxidation of Oxybenzone and Oxidation Products.]]

← Older revision		Revision as of 20:12, 1 April 2020
Line 65:		Line 65:
	[[File:oxybenzone_021420_oxidation.jpg\|400px\|thumb\|right\|Figure 2. Graph depicting the decrease in oxybenzone as the concentration of potassium permanganate increases.]]		[[File:oxybenzone_021420_oxidation.jpg\|400px\|thumb\|right\|Figure 2. Graph depicting the decrease in oxybenzone as the concentration of potassium permanganate increases.]]

−	[[File:posterpicture_research.jpg\|~~400px~~\|thumb\|Figure 3. Representation of the Oxidation of Oxybenzone and Oxidation Products.]]	+	[[File:posterpicture_research.jpg\|600px\|thumb\|left\|Figure 3. Representation of the Oxidation of Oxybenzone and Oxidation Products.]]

@@ Line 63: / Line 63: @@
 [[File:oxyb.png|400px|thumb|left|Figure 1. Graph of oxybenzone dissolved in methanol.]]
-[[File:oxybenzone_021420_oxidation.jpg|400pxthumb|center|Figure 2. Graph depicting the decrease in oxybenzone as the concentration of potassium permanganate increases.]]
+[[File:oxybenzone_021420_oxidation.jpg|400px|thumb|right|Figure 2. Graph depicting the decrease in oxybenzone as the concentration of potassium permanganate increases.]]
-[[File:posterpicture_research.jpg|400px|thumb|right|Figure 3. Representation of the Oxidation of Oxybenzone and Oxidation Products.]]
+[[File:posterpicture_research.jpg|400px|thumb|Figure 3. Representation of the Oxidation of Oxybenzone and Oxidation Products.]]

← Older revision		Revision as of 20:11, 1 April 2020
Line 62:		Line 62:
	==Data==		==Data==
	[[File:oxyb.png\|400px\|thumb\|left\|Figure 1. Graph of oxybenzone dissolved in methanol.]]		[[File:oxyb.png\|400px\|thumb\|left\|Figure 1. Graph of oxybenzone dissolved in methanol.]]
		+
		+	[[File:oxybenzone_021420_oxidation.jpg\|400pxthumb\|center\|Figure 2. Graph depicting the decrease in oxybenzone as the concentration of potassium permanganate increases.]]
		+
		+	[[File:posterpicture_research.jpg\|400px\|thumb\|right\|Figure 3. Representation of the Oxidation of Oxybenzone and Oxidation Products.]]