Ovoperoxidase from Sea Urchins - Revision history

Jwelker: /* Scots J-Term Andros Island */

2020-06-30T20:03:21Z

Scots J-Term Andros Island

Wfox: /* Sea Urchin Sperm Peroxidase */

2019-07-09T21:38:38Z

Sea Urchin Sperm Peroxidase

Jwelker: /* Project Overview */

2019-07-09T21:37:35Z

Project Overview

Jwelker: /* Project Overview */

2019-07-09T21:37:23Z

Project Overview

Jwelker: /* Project Overview */

2019-07-09T21:36:50Z

Project Overview

Jwelker at 21:30, 9 July 2019

2019-07-09T21:30:49Z

Jwelker: /* References */

2019-07-09T21:30:02Z

References

Jwelker: /* References */

2019-07-09T21:29:06Z

References

Jwelker: /* Preparation */

2019-07-09T21:24:13Z

Preparation

Jwelker at 21:21, 9 July 2019

2019-07-09T21:21:13Z

@@ Line 97: / Line 97: @@
 ==Scots J-Term Andros Island==
-January 4th through January 12th of 2019 my research advisor, Brad Sturgeon, along with 7 other students and myself, visited Forfar Field Station on Andros Island in the Bahamas. There I was able to learn about the natural environment of sea urchins We also gained field experience by studying them through snorkeling. The interns at Forfar were very knowledgeable about the area and the creatures inhabiting the ocean around the island.
+January 4th through January 12th of 2019 my research advisor, Brad Sturgeon, along with seven other students and myself, visited Forfar Field Station on Andros Island in the Bahamas. There I was able to learn about the natural environment of sea urchins, as well as other relevant science such as geology. We also gained field experience by studying them through snorkeling. The interns at Forfar were very knowledgeable about the area and the creatures inhabiting the ocean around the island. Emily Rein and Will Fox, who are involved in different aspects of the sea urchin research, were among the seven students on the trip.
 Below is a link to an article written about the trip as a whole:
 [http://monmouthcollege.meritpages.com/news/monmouth-college-students--explore-new-worlds--on-one-week-academic-trip-to-the-bahamas/7194?fbclid=IwAR3JOLqcFYNKls6f7rsYAbn1_zJ195XaUMS-ev_IjFWNxHeGl177VBKaLY8| Bahamas Trip]

← Older revision		Revision as of 21:38, 9 July 2019
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	==Sea Urchin Sperm Peroxidase==		==Sea Urchin Sperm Peroxidase==
		+
		+	When a Sea Urchin egg is fertilized, it releases hydrogen peroxide into the surrounding space. This peroxide interacts with a peroxidase endogenous to the sperm to render the sperm infertile, and in doing so helps prevent polyspermy.

	==Preparation==		==Preparation==

@@ Line 1: / Line 1: @@
 ==Project Overview==
-The main goal of this project is to isolate the sea urchin ovoperoxidase enzyme and collect data about it's activity and reactions with hydrogen peroxide. Former student Laura Sanchez was isolating the protein, but upon graduation her work was passed down to Emily Rein who will continue to work on isolating the enzyme for our use. Another student, [[William_B_Fox|Will Fox]] will be assisting with the synthesis and analysis of the sperm peroxidase.
+The main goal of this project is to isolate the sea urchin ovoperoxidase enzyme and collect data about it's activity and reactions with hydrogen peroxide. Former student Laura Sanchez was isolating the protein, but upon graduation her work was passed down to Emily Rein who will continue to work on isolating the enzyme for our use. Another student, [[William_B_Fox|Will Fox,]] will be assisting with the synthesis and analysis of the sperm peroxidase.
 The following video portrays the actual fertilization process for sea urchins:

@@ Line 1: / Line 1: @@
 ==Project Overview==
-The main goal of this project is to isolate the sea urchin ovoperoxidase enzyme and collect data about it's activity and reactions with hydrogen peroxide. Former student Laura Sanchez was isolating the protein, but upon graduation her work was passed down to Emily Rein who will continue to work on isolating the enzyme for our use. Another student, [Will_Fox|Will Fox] will be assisting with the synthesis and analysis of the sperm peroxidase.
+The main goal of this project is to isolate the sea urchin ovoperoxidase enzyme and collect data about it's activity and reactions with hydrogen peroxide. Former student Laura Sanchez was isolating the protein, but upon graduation her work was passed down to Emily Rein who will continue to work on isolating the enzyme for our use. Another student, [[William_B_Fox|Will Fox]] will be assisting with the synthesis and analysis of the sperm peroxidase.
 The following video portrays the actual fertilization process for sea urchins:

@@ Line 1: / Line 1: @@
 ==Project Overview==
-The main goal of this project is to isolate the sea urchin ovoperoxidase enzyme and collect data about it's activity and reactions with hydrogen peroxide. Former student Laura Sanchez was isolating the protein, but upon graduation her work was passed down to Emily Rein who will continue to work on isolating the enzyme for our use.
+The main goal of this project is to isolate the sea urchin ovoperoxidase enzyme and collect data about it's activity and reactions with hydrogen peroxide. Former student Laura Sanchez was isolating the protein, but upon graduation her work was passed down to Emily Rein who will continue to work on isolating the enzyme for our use. Another student, [Will_Fox|Will Fox] will be assisting with the synthesis and analysis of the sperm peroxidase.
 The following video portrays the actual fertilization process for sea urchins:

← Older revision		Revision as of 21:30, 9 July 2019
Line 32:		Line 32:

	Ovoperoxidase is an enzyme, released from eggs at fertilization, that creates a fertilization envelope around the sea urchin egg by to prevent multiple sperm from fertilizing the egg. If multiple sperm reach the egg, the egg will not become fertilized. This hardening process is due to the oxidation of protein-bound tyrosyl residues, which happens in the presence of a peroxidase.		Ovoperoxidase is an enzyme, released from eggs at fertilization, that creates a fertilization envelope around the sea urchin egg by to prevent multiple sperm from fertilizing the egg. If multiple sperm reach the egg, the egg will not become fertilized. This hardening process is due to the oxidation of protein-bound tyrosyl residues, which happens in the presence of a peroxidase.
		+
		+	==Sea Urchin Sperm Peroxidase==

	==Preparation==		==Preparation==

@@ Line 90: / Line 90: @@
 [https://www.pnas.org/content/pnas/74/10/4214.full.pdf Tyrosine Crosslinks]
-[http://Sea_urchin_sperm_peroxidase Sea Urchin Sperm Peroxidase]
+[[:File:Sea_urchin_sperm_peroxidase_is_competitively_inhib.pdf| Sea Urchin Sperm Peroxidase]]
 ==Scots J-Term Andros Island==

← Older revision		Revision as of 21:29, 9 July 2019
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	[https://www.pnas.org/content/pnas/74/10/4214.full.pdf Tyrosine Crosslinks]		[https://www.pnas.org/content/pnas/74/10/4214.full.pdf Tyrosine Crosslinks]
		+
		+	[http://Sea_urchin_sperm_peroxidase Sea Urchin Sperm Peroxidase]

	==Scots J-Term Andros Island==		==Scots J-Term Andros Island==

← Older revision		Revision as of 21:24, 9 July 2019
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	==Preparation==		==Preparation==
		+	In preparation for the egg extraction, sea water was made with a specific gravity of 1.015, which was measured with a refractometer. Some of this sea water was combined with the water the sea urchins came in and poured in an aquarium with a 50/50 mixture. This aquarium is where the urchins were placed after they were done releasing their gametes. The rest of the sea water was saved for use in the cups the urchins were placed in to release gametes.

	==Egg Extraction==		==Egg Extraction==

← Older revision		Revision as of 21:21, 9 July 2019
Line 32:		Line 32:

	Ovoperoxidase is an enzyme, released from eggs at fertilization, that creates a fertilization envelope around the sea urchin egg by to prevent multiple sperm from fertilizing the egg. If multiple sperm reach the egg, the egg will not become fertilized. This hardening process is due to the oxidation of protein-bound tyrosyl residues, which happens in the presence of a peroxidase.		Ovoperoxidase is an enzyme, released from eggs at fertilization, that creates a fertilization envelope around the sea urchin egg by to prevent multiple sperm from fertilizing the egg. If multiple sperm reach the egg, the egg will not become fertilized. This hardening process is due to the oxidation of protein-bound tyrosyl residues, which happens in the presence of a peroxidase.
		+
		+	==Preparation==

	==Egg Extraction==		==Egg Extraction==