N-Acetyl L-Tyrosine - Revision history

Benstillwell: /* Flash Chromatography */

2016-07-24T18:54:10Z

Flash Chromatography

2016-07-24T18:40:37Z

Flash Chromatography

2016-07-24T18:38:52Z

Flash Chromatography

2016-07-24T18:38:08Z

Flash Chromatography

2016-07-24T18:37:48Z

2016-07-24T18:36:50Z

Flash Chromatography

2016-07-24T18:36:33Z

Flash Chromatography

2016-07-24T18:32:55Z

Flash Chromatography

2016-07-24T18:30:36Z

Flash Chromatography

2016-07-21T18:58:12Z

← Older revision		Revision as of 18:54, 24 July 2016
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	[[File:FLASHf.PNG\|500px\|thumb\|none\|Flash Figure 3: 20mM NAT ran through flash with the wavelength red and purple being 223 and 276, respectively.]]		[[File:FLASHf.PNG\|500px\|thumb\|none\|Flash Figure 3: 20mM NAT ran through flash with the wavelength red and purple being 223 and 276, respectively.]]
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		+
		+	Analysis of Flash Data: While having the axes at different magnitudes can make this flash data confusing, it is important to understand the purpose of flash, which is separation. When running the flash at a wavelength of 223 as opposed to 276, more of a peak is put into one test tube. The same was true when running the flash at 9ml/min as opposed to 18ml/min. The most favorable combination of flow rate and wavelength was Flash Figure 3, with wavelengths of 223 and 276 and with a flow rate of 9ml/min.
		+
		+	For the Future: The next step moving forward is to use this information from the NAT standard to run a sample through the flash that has been reacted with peroxide and HRP. Then, by isolating these products, they can be then again run through the HPLC to test how pure the isolated samples are. Once isolated samples are acquired, running an H-NMR scan on the isolated and purified products will help to identify exactly what are the products from the oxidation of N-Acetyl L-Tyrosine.

	==Antioxidants in Reaction==		==Antioxidants in Reaction==

@@ Line 33: / Line 33: @@
 [[File:NAT223.jpg|500px|thumb|none|HPLC chromatogram of 20mM NAT.]]
-[[File:FLASHa.PNG|500px|thumb|none|20mM NAT ran through flash with the wavelength red and purple being 276 and 300, respectively.]]
+[[File:FLASHa.PNG|500px|thumb|none|Flash Figure 1: 20mM NAT ran through flash with the wavelength red and purple being 276 and 300, respectively.]]
-[[File:FLASHe.PNG|500px|thumb|none|20mM NAT ran through flash with the wavelength red and purple being 223 and 276, respectively.]]
+[[File:FLASHe.PNG|500px|thumb|none|Flash Figure 2: 20mM NAT ran through flash with the wavelength red and purple being 223 and 276, respectively.]]
-The following graph shows a difference between
+The following graph shows a difference between the flow rates of Flash Figure 2 and Flash Figure 3.  Flash figure 3 was ran at a speed of 18ml/min while Flash Figure 3 was ran at 9ml/min.
 ==Antioxidants in Reaction==

← Older revision		Revision as of 18:38, 24 July 2016
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	[[File:FLASHe.PNG\|500px\|thumb\|none\|20mM NAT ran through flash with the wavelength red and purple being 223 and 276, respectively.]]		[[File:FLASHe.PNG\|500px\|thumb\|none\|20mM NAT ran through flash with the wavelength red and purple being 223 and 276, respectively.]]
		+
		+
		+	The following graph shows a difference between

	==Antioxidants in Reaction==		==Antioxidants in Reaction==

@@ Line 27: / Line 27: @@
 The objective of flash chromatography is to isolate peaks seen in the HPLC.  Running flash chromatography allows for the product to be isolated.
 [[File:DK 11.jpg|500px|thumb|none|Figure 6: Flash chromatography separation of the 10mM H2O2 in Figure 5 6/28/16]]
-==
 The following graphs show the difference between running the flash system under conditions with wavelength at 276 and 300 with 223 and 276.  The first graph is the NAT standard ran through the HPLC.  The two flash chromatography graphs show the difference in changing the methods of the flash system.
 [[File:NAT223.jpg|500px|thumb|none|HPLC chromatogram of 20mM NAT.]]

@@ Line 27: / Line 27: @@
 The objective of flash chromatography is to isolate peaks seen in the HPLC.  Running flash chromatography allows for the product to be isolated.
 [[File:DK 11.jpg|500px|thumb|none|Figure 6: Flash chromatography separation of the 10mM H2O2 in Figure 5 6/28/16]]
-====
+==
 The following graphs show the difference between running the flash system under conditions with wavelength at 276 and 300 with 223 and 276.  The first graph is the NAT standard ran through the HPLC.  The two flash chromatography graphs show the difference in changing the methods of the flash system.
 [[File:NAT223.jpg|500px|thumb|none|HPLC chromatogram of 20mM NAT.]]

← Older revision		Revision as of 18:58, 21 July 2016
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−	This page depicts the oxidation of N-Acetyl L-Tyrosine (NAT) by Horse Radish Peroxidase (HRP). Each sample of NAT was dissolved pH 5 phosphate citrate buffer. The method used on the HPLC was "HPA_0604156".	+	This page depicts the oxidation of N-Acetyl L-Tyrosine (NAT) by Horse Radish Peroxidase (HRP). Each sample of NAT was dissolved in pH 5 phosphate citrate buffer. The method used on the HPLC was "HPA_0604156".
	[[File:NAT.png\|200px\|thumb\|none\|NAT]]		[[File:NAT.png\|200px\|thumb\|none\|NAT]]