Morgan Gulley Chem430 F16 - Revision history

Mgulley at 20:46, 20 January 2017

2017-01-20T20:46:57Z

Mgulley: /* Research Times */

2017-01-20T20:45:57Z

Research Times

Mgulley: /* Research Times */

2016-09-06T22:55:16Z

Research Times

Mgulley: /* Research Times */

2016-09-06T22:54:50Z

Research Times

Mgulley: /* Research Times */

2016-09-06T22:54:29Z

Research Times

Mgulley: /* Proposed Research Project */

2016-09-06T22:54:05Z

Proposed Research Project

Mgulley: /* Proposal for Research */

2016-09-06T22:51:55Z

Proposal for Research

Mgulley: /* References (2 minimum) */

2016-09-06T22:47:35Z

References (2 minimum)

Mgulley: /* Proposed Research Project */

2016-09-06T22:47:05Z

Proposed Research Project

Mgulley: Created page with "Chemistry/Biochemistry Research 430 :Fall 2016 :Morgan Gulley :Senior Chemistry Major with Math & Physics Minors ==Research Times== Tuesdays from 2-6 pm in the Trough Lab : s..."

2016-08-28T02:02:10Z

Created page with "Chemistry/Biochemistry Research 430 :Fall 2016 :Morgan Gulley :Senior Chemistry Major with Math & Physics Minors ==Research Times== Tuesdays from 2-6 pm in the Trough Lab : s..."

New page

Chemistry/Biochemistry Research 430
:Fall 2016
:Morgan Gulley
:Senior Chemistry Major with Math & Physics Minors

==Research Times==
Tuesdays from 2-6 pm in the Trough Lab
: section 01 = 0.25 credit = 4 hours per week.

==Proposed Research Project==
===Enter Project Title here===

===General Information===
:Advisor: Audra Sostarecz
:Other research student collaborators: Khader
:Other Research Collaborators: NONE

===Proposal===

===Instruments to be used===
:Langmuir Monolayer Trough
:ezAFM
===References (2 minimum)===
:Mehdi Kazemzadeh-Narbat, Benjamine F.L. Lai, Chuanfan Ding, Jayachandran N. Kizhakkedathu, Robert E.W. Hancock, and Rizhi Wang. “Multilayered Coating on Titanium for Controlled Release of Antimicrobial Peptides for the Prevention of Implant-Associated Infections.” Biomaterials 34 (2013): 5969–77.
:Jeanette Libera, Thomas Pomorski, Oliviera Josimovic-Alasevic, Karl-Gerd Fritsch, and Andreas Herrmann. “Internalization of Phospholipids from the Plasma Membrane of Human Osteoblast Depends on the Lipid Head Group.” Journal of Bone and Mineral Research 14, no. 5 (1999): 690–99.

===Research pledge===
I, Morgan Gulley, have read the Chem/Bioc 430 course syllabus and understand the general structure and expectations of the research program. The above material was prepared after consultation, and in conjunction with my research advisor, Audra Sostarecz.

@@ Line 5: / Line 5: @@
 ==Research Times==
-:Tuesdays from 2-6 pm in the Trough Lab
+:Tuesdays from 1-5 pm in the Trough Lab
 : section 01 = 0.25 credit = 4 hours per week.

@@ Line 5: / Line 5: @@
 ==Research Times==
-;Tuesdays from 2-6 pm in the Trough Lab
+:Tuesdays from 2-6 pm in the Trough Lab
 : section 01 = 0.25 credit = 4 hours per week.

@@ Line 17: / Line 17: @@
 ===Proposal for Research===
-:Langmuir-Blodgett Monolayers of collagen and phospholipids are being used as a model system for the affinity of osteoblasts to titanium rods.  The Langmuir Monolayer technique allows for the analysis of the organization of amphiphilic molecules at an air-water interface and is, therefore, a useful technique for the formation of model cell membranes. Collagen, Type I from calf skin, will be used as a substitute for human bone and titanium nitride foil will be used as the substrate due to its increased biocompatibility.  Langmuir monolayers of collagen are found to have a higher degree of order (less fluidity) and to be more stable indicated by a higher surface pressure at low molecular areas when incorporated into a film with phospholipids such as dipalmitoylphosphatidylcholine (DPPC) and dipalmitoylphosphatidylethanolamine (DPPE).  Similarly, transfer ratio data of collagen monolayers to silicon (SiO2) substrates increased in the presence of DPPC and DPPE indicating that this may be a good model system for this investigation.  In preparation for current experiments involving Atomic Force Microscopy analysis of collagen/phospholipid monolayers transferred to titanium nitride foil using dipalmitolyphosphatidylglycerol (DPPG) as the lipid because of its impact on bacterial resistance in the bone cell model membrane, we have determined that we can use the ezAFM for analysis of transferred multilayers of arachidic acid. This will be completed after preliminary studies of trasnferring DPPC or DPPE with collagen onto the titanium nitride foil.
+:Langmuir-Blodgett Monolayers of collagen and phospholipids will be used as a model system for the affinity of osteoblasts to titanium rods.  The Langmuir Monolayer technique allows for the analysis of the organization of amphiphilic molecules at an air-water interface and is, therefore, a useful technique for the formation of model cell membranes. Collagen, Type I from calf skin, will be used as a substitute for human bone and titanium nitride foil will be used as the substrate due to its increased biocompatibility.  Langmuir monolayers of collagen are found to have a higher degree of order (less fluidity) and to be more stable indicated by a higher surface pressure at low molecular areas when incorporated into a film with phospholipids such as dipalmitoylphosphatidylcholine (DPPC) and dipalmitoylphosphatidylethanolamine (DPPE).  Similarly, transfer ratio data of collagen monolayers to silicon (SiO2) substrates increased in the presence of DPPC and DPPE indicating that this may be a good model system for this investigation. These studies were completed last semester.  In preparation for current experiments involving Atomic Force Microscopy analysis of collagen/phospholipid monolayers transferred to titanium nitride foil using dipalmitolyphosphatidylglycerol (DPPG) as the lipid because of its impact on bacterial resistance in the bone cell model membrane, we have determined that we can use the ezAFM for analysis of transferred multilayers of arachidic acid. It was also determined last semester that a liquid expands/liquid condensed phase of DPPC transferred onto SiO2 by AFM analysis. With this information present, the transfer of a collagen/phospholipid monolayer should be possible, and will be tested.
 ===Instruments to be used===

@@ Line 1: / Line 1: @@
 Chemistry/Biochemistry Research 430
-:Fall 2016
+:Spring 2017
 :Morgan Gulley
 :Senior Chemistry Major with Math & Physics Minors

@@ Line 8: / Line 8: @@
 : section 01 = 0.25 credit = 4 hours per week.
-==Proposed Research Project==
+==General Information==
 :Advisor: Audra Sostarecz
 :Other research student collaborators: Khader
 :Other Research Collaborators: NONE
-===Proposal for Research===
+==Proposed Research Project==
 :Langmuir-Blodgett Monolayers of collagen and phospholipids will be used as a model system for the affinity of osteoblasts to titanium rods.  The Langmuir Monolayer technique allows for the analysis of the organization of amphiphilic molecules at an air-water interface and is, therefore, a useful technique for the formation of model cell membranes. Collagen, Type I from calf skin, will be used as a substitute for human bone and titanium nitride foil will be used as the substrate due to its increased biocompatibility.  Langmuir monolayers of collagen are found to have a higher degree of order (less fluidity) and to be more stable indicated by a higher surface pressure at low molecular areas when incorporated into a film with phospholipids such as dipalmitoylphosphatidylcholine (DPPC) and dipalmitoylphosphatidylethanolamine (DPPE).  Similarly, transfer ratio data of collagen monolayers to silicon (SiO2) substrates increased in the presence of DPPC and DPPE indicating that this may be a good model system for this investigation. These studies were completed last semester.  In preparation for current experiments involving Atomic Force Microscopy analysis of collagen/phospholipid monolayers transferred to titanium nitride foil using dipalmitolyphosphatidylglycerol (DPPG) as the lipid because of its impact on bacterial resistance in the bone cell model membrane, we have determined that we can use the ezAFM for analysis of transferred multilayers of arachidic acid. It was also determined last semester that a liquid expands/liquid condensed phase of DPPC transferred onto SiO2 by AFM analysis. With this information present, the transfer of a collagen/phospholipid monolayer should be possible, and will be tested.
-===Instruments to be used===
+==Instruments to be used==
-:Langmuir Monolayer Trough
+:Langmuir-Blodgett Monolayer Trough
 :ezAFM
-===References===
 :Mehdi Kazemzadeh-Narbat, Benjamine F.L. Lai, Chuanfan Ding, Jayachandran N. Kizhakkedathu, Robert E.W. Hancock, and Rizhi Wang. “Multilayered Coating on Titanium for Controlled Release of Antimicrobial Peptides for the Prevention of Implant-Associated Infections.” Biomaterials 34 (2013): 5969–77.
 :Jeanette Libera, Thomas Pomorski, Oliviera Josimovic-Alasevic, Karl-Gerd Fritsch, and Andreas Herrmann. “Internalization of Phospholipids from the Plasma Membrane of Human Osteoblast Depends on the Lipid Head Group.” Journal of Bone and Mineral Research 14, no. 5 (1999): 690–99.
-===Research pledge===
+==Research pledge==
 I, Morgan Gulley, have read the Chem/Bioc 430 course syllabus and understand the general structure and expectations of the research program. The above material was prepared after consultation, and in conjunction with my research advisor, Audra Sostarecz.

@@ Line 9: / Line 9: @@
 ==Proposed Research Project==
-===Enter Project Title here===
+===Langmuir Monolayer and AFM Analysis of a Collagen/Phospholipid/Titanium Model Membrane System for the Investigation of Osteoblast Affinity to Titanium Rods===
 ===General Information===
@@ Line 16: / Line 16: @@
 :Other Research Collaborators: NONE
-===Proposal===
+===Proposal for Research===
 ===Instruments to be used===