Mohannadfa: Created page with "Chemistry Research 430 :Spring 2017 :Mohanad Ahmad :Senior Chemistry Major ==Research Times== T/W 2-6 pm : section 02 = 0.50 credit = 8 hours per week. ==Proposed Research P..."

2017-02-11T20:24:12Z

Created page with "Chemistry Research 430 :Spring 2017 :Mohanad Ahmad :Senior Chemistry Major ==Research Times== T/W 2-6 pm : section 02 = 0.50 credit = 8 hours per week. ==Proposed Research P..."

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Chemistry Research 430
:Spring 2017
:Mohanad Ahmad
:Senior Chemistry Major

==Research Times==
T/W 2-6 pm
: section 02 = 0.50 credit = 8 hours per week.

==Proposed Research Project==
===Attachment of DNA to cAMP transcription factor: Atomic Force Microscopy study===

===General Information===
:Advisor: Dr. Laura Moore
:Other research student collaborators: Khdr Eskander

===Proposal===
The goal of this project is to introduce of a strand of DNA that carries a specific mutation into E.coli bacteria in order to over express the bacteria to synthesize the mutated c-AMP receptor protein (CRP), a transcription factor that regulates DNA to mRNA transcription. The intended mutation will expose a cysteine amino acid on the surface of the protein that will, as a result, bind to a gold surface through a thiol bond (R-S-H) in order to obtain images using Atomic Force Microscopy (AFM). Moreover, images of DNA-CRP complex will be taken using AFM, where the DNA will be attached to the tip of the AFM at one end and to the protein at the other end.

===Instruments to be used===
Atomic Force Microscopy

===References===
Ditzler, Lindsay R., Arundhuti Sen, Michael J. Gannon, Amnon Kohen, Alexei V. Tivanski. "Self-Assembled Enzymatic Monolayer Directly Bound to a Gold Surface: Activity and Molecular Recognition Force Spectroscopy Studies." ''J. Am. Chem. Soc'', 2011, 133 (34), pp 13284–13287.

===Research pledge===
I, Mohanad Ahmad, have read the Chem/Bioc 430 course syllabus and understand the general structure and expectations of the research program. The above material was prepared after consultation, and in conjunction with my research advisor.

Mohanad Ahmad Chem430 S17 - Revision history

Mohannadfa: Created page with "Chemistry Research 430 :Spring 2017 :Mohanad Ahmad :Senior Chemistry Major ==Research Times== T/W 2-6 pm : section 02 = 0.50 credit = 8 hours per week. ==Proposed Research P..."