HPA Reactions - Revision history

Nayala at 02:44, 18 November 2016

2016-11-18T02:44:21Z

2016-11-17T13:42:22Z

Product Detection Test

2016-11-17T13:41:48Z

Combinations

2016-07-21T18:27:27Z

The combination of these radicals are the expected products of the reaction and can begin as a combination of the following dimers

2016-07-21T18:26:13Z

The combination of these radicals are the expected products of the reaction and can begin as a combination of the following dimers

2016-07-21T18:12:50Z

Enzyme Oxidation of HPA

2016-07-21T18:12:17Z

Enzyme Oxidation of HPA

2016-07-21T18:11:21Z

Enzyme Oxidation of HPA

2016-07-21T17:59:10Z

2016-07-14T19:17:45Z

NMR Results

← Older revision		Revision as of 02:44, 18 November 2016
Line 116:		Line 116:

	After collecting samples through Flash Chromatography, we are able to identify the proton structure of a product using NMR.		After collecting samples through Flash Chromatography, we are able to identify the proton structure of a product using NMR.
		+	[[File:NMR full.png\|700px\|thumb\|none\|NMR of Flash #3 peak 3 (7/13/16)]]
	[[File:NMR product 1.png\|700px\|thumb\|none\|NMR of Flash #3 peak 3 (7/13/16)]]		[[File:NMR product 1.png\|700px\|thumb\|none\|NMR of Flash #3 peak 3 (7/13/16)]]

@@ Line 25: / Line 25: @@
 [[File:HPA A-B Dimer 01.png|thumb|none|A-B Dimer]]
-==Product Detection Test==
+==Product Identification==
 Reaction of 50/50 HPA-Dioxane Solution in Buffer with various H2O2 concentrations and Horse Radish Peroxidase (HRP)
@@ Line 52: / Line 52: @@
 Immediately after the IE HRP HPA/H2O2 (6/27/16) was run, the bioreactor was cleaned with MOPS buffer in preparation for a second run. At a flow rate of 0.25mL/min and 20mM HPA + Dioxide and 20mM H2O2 conditions, the products of the run where expected to behave in the same way as the trial run previously. However it was seen that the products of the second trial have a lower concentration than that of the previous run. [[File:IE HPA10 04.jpg|500px|thumb|center|IE HRP HPA/H2O2 (6/27/16)-2]]
 This change bring into question the change in the conditions within the bioreactor when used continually.
 ==Concentration Test==

@@ Line 10: / Line 10: @@
-===Combinations===
+===Radical-Radical Recombinations===
 The combination of these radicals are the expected products of the reaction and can begin as a combination of the following dimers.
 An A-A Dimer would create an Organic Peroxide, in which the oxygen radicals create a bond.

@@ Line 10: / Line 10: @@
-===The combination of these radicals are the expected products of the reaction and can begin as a combination of the following dimers===
+===Combinations===
+The combination of these radicals are the expected products of the reaction and can begin as a combination of the following dimers.
 An A-A Dimer would create an Organic Peroxide, in which the oxygen radicals create a bond.
 [[File:HPA A-A Dimer .png|thumb|none|A-A Dimer]]

@@ Line 18: / Line 18: @@
 A B-B Dimer creates a Carbon-Carbon bond between the 3rd carbon of each HPA monomers. This is also commonly refereed to as a 3-3' bond and is the same as a D-D dimer in that the D radical is on the mirror opposite of the the C radical.
 [[File:HPA B-B Dimer 01.png|thumb|none|B-B Dimer]]
 ==Product Detection Test==

← Older revision		Revision as of 18:12, 21 July 2016
Line 10:		Line 10:


−	The combination of these radicals are the expected products of the reaction and can begin as a combination of the following dimers:	+	===The combination of these radicals are the expected products of the reaction and can begin as a combination of the following dimers===

	An A-A Dimer would create an Organic Peroxide, in which the oxygen radicals create a bond.		An A-A Dimer would create an Organic Peroxide, in which the oxygen radicals create a bond.

@@ Line 7: / Line 7: @@
 The oxidation of HPA forms radicals which form resonance structures and can be combined with each other to form dimers, trimes, and other polymers as a result of oxidation and reduction through an enzyme mechanism.
 [[File:HPA radicals.png|500px|thumb|none|HPA Radicals]]
 ==Product Detection Test==

@@ Line 1: / Line 1: @@
 -Hydroxyphenylacetic acid (HPA)
 [[File:4-Hydroxyphenylacetic acid.svg.png|200px|thumb|none|HPA]]
 ==Product Detection Test==
 Reaction of 50/50 HPA-Dioxane Solution in Buffer with various H2O2 concentrations and Horse Radish Peroxidase (HRP)
 [[File:Layout HPA 02.jpg|500px|thumb|Left|HPA/H2O2/HPA (6/3/16)]]

← Older revision		Revision as of 19:17, 14 July 2016
Line 92:		Line 92:
	==NMR Results==		==NMR Results==

−	[[File:NMR product 1.png\|~~500px~~\|thumb\|none\|NMR of Flash #3 peak 3 (7/13/16)]]	+	After collecting samples through Flash Chromatography, we are able to identify the proton structure of a product using NMR.
		+	[[File:NMR product 1.png\|700px\|thumb\|none\|NMR of Flash #3 peak 3 (7/13/16)]]