Flow Cell Lab Activity - Revision history

Bes: /* Introduction to Flow Cells */

2018-02-22T18:14:51Z

Introduction to Flow Cells

Bes: /* Introduction to Flow Cells */

2018-02-22T18:02:40Z

Introduction to Flow Cells

Bes: /* Introduction to Flow Cells */

2018-02-22T17:56:15Z

Introduction to Flow Cells

Bes: /* Flow Cell Construction */

2017-02-18T14:58:18Z

Flow Cell Construction

Bes: /* Silicon Casting */

2017-02-18T14:56:12Z

Silicon Casting

Bes: /* 3d Printing */

2017-02-18T14:52:41Z

3d Printing

Bes: /* Prep for 3d Printing */

2017-02-18T14:50:21Z

Prep for 3d Printing

Bes: /* Producing The Virtual Object */

2017-02-18T14:48:49Z

Producing The Virtual Object

Bes: /* Introduction to Flow Cells */

2017-02-18T14:48:04Z

Introduction to Flow Cells

Bes: /* Introduction to Flow Cells */

2017-02-18T14:42:58Z

Introduction to Flow Cells

← Older revision		Revision as of 18:14, 22 February 2018
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	[https://www.youtube.com/watch?v=AENnU0vdjPo A microfluidic device. separation,sorting,mixing]		[https://www.youtube.com/watch?v=AENnU0vdjPo A microfluidic device. separation,sorting,mixing]
		+
		+	[https://www.youtube.com/watch?v=D72_ZBqa6tY Lab on a chip.wmv]

	==Producing The Virtual Object==		==Producing The Virtual Object==

← Older revision		Revision as of 18:02, 22 February 2018
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	[https://www.youtube.com/watch?v=CpkXmtJOH84 Geraldine Hamilton: Body parts on a chip]		[https://www.youtube.com/watch?v=CpkXmtJOH84 Geraldine Hamilton: Body parts on a chip]
		+
		+	[https://www.youtube.com/watch?v=AENnU0vdjPo A microfluidic device. separation,sorting,mixing]

	==Producing The Virtual Object==		==Producing The Virtual Object==

← Older revision		Revision as of 17:56, 22 February 2018
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	https://youtu.be/UpJ-kGII074		https://youtu.be/UpJ-kGII074
		+
		+
		+	[https://www.youtube.com/watch?v=CpkXmtJOH84 Geraldine Hamilton: Body parts on a chip]

	==Producing The Virtual Object==		==Producing The Virtual Object==

@@ Line 65: / Line 65: @@
 ==Flow Cell Construction==
-After completing the casting, the mold was placed on two glass slides. This created a sandwich in which solution could pass through. Holes were drilled on one of the glass slide, corresponding to each model using a driller of size 2mm. Adapters between the slide holes and mold model were utilized to allow the solutions inside. Solutions were made using color dyes blue and yellow in 500 ml of water. A waste beaker was used to collect the mixed solutions.
+After completing the casting, the mold was placed on two acrylic slides. This created a sandwich in which solution could pass through. Holes were drilled on one of the glass slide, corresponding to each model using a bit of size 2 mm. Luer adapters were installed to allow the solutions to flow. Solutions were made using color dyes blue and yellow in 500 ml of water. A waste beaker was used to collect the mixed solutions. Peristaltic pumps or syringe drive pumps were used to pump the colored solutions into the flow cell.
 The rate of pumping differed in every model.
 ===[[File:Brads Flow Cell.PNG|200px]]===

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 ==Silicon Casting==
-After the 3D-printed cast has been made, now the actual flow cell can be created. We have used a 10:1 ratio of Slygard 184 (a silicon monomer) to curating agent, or about 5 grams Slygard 184 to 0.5 grams curating agent. After this is mixed thoroughly, we pour it into the cast and place it in a desiccator and place a vacuum on it to let the air bubbles come to the surface; once the vacuum is taken off, the bubbles will pop if left long enough.
+After the 3D-printed cast has been made, now the actual flow cell can be created. We have used a 10:1 ratio of Slygard 184 (a silicon monomer) to curating agent, or about 5 grams Slygard 184 to 0.5 grams curating agent. After this is mixed thoroughly, we pour it into the cast and place it in a vacuum desiccator to remove air bubbles in the silicone, ~10 mins.
 [[File:Cast in desiccator.jpg|200px|thumb|center|Silicon mold in Desiccator]]
-After this, we can leave the cast to curate, or let the silicon harden and shape to the cast. There were a few ways this was done. Some put their cast into the oven, but due to the low melting temperature of the acrylic plastic, they started to melt, making them unable to be reused. Others, however, left theirs out over the time span of a week to harden. A table of how they have been done is listed below.
+After this, we leave the cast to cure/let the silicon harden. There were a few ways this was done. Some put their cast into the oven, but due to the low melting temperature of the PLA plastic, they started to melt, making them unable to be reused. Others, however, left theirs out over the time span of a week to harden. A table of how they have been done is listed below.
 [[File:Screen Shot 2016-04-07 at 4.01.54 PM.png|600px|thumb|center|Curing Times]]

@@ Line 52: / Line 52: @@
 ==3d Printing==
+Gcode file needs to be save to a SD card (available at printer) in order to print on the Prusa i3 3D printer located in the NMR room.
 ==Silicon Casting==

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 ==Prep for 3d Printing==
-A gcode file is required in order to print an object on the 3D printer. This file can be created by exporting one's Tinkercad object as an STL. The STL file can be imported into Matter Control.
+A gcode file is required in order to print an object on the 3D printer. This file can be created by exporting one's Tinkercad object as an STL. The STL file can be imported into "Matter Control" or "Slicer" to generate the gcode file.
 The gcode file contains settings specific to the 3D printer being used in production of the flow cell. As a result, one must ensure that the proper printer, material, and settings are chosen before exportation of the final gcode.

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 * Click on Create a New Design to get started.
 * There are tutorials available in TinkerCAD to get used to object placements and using different shapes. Also refer this link (https://www.tinkercad.com/quests/)
 ===Dimensions of the Mold===
 * The Outer Box : 56 mm X 26 mm with a height of 7 mm with a 2 mm bottom
 * Interior Channels : Channel height is 2 mm and the width of each channel is 2 mm
 ===Things to Note===

← Older revision		Revision as of 14:48, 18 February 2017
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	Chemist do a lot of mixing and it is generally assumed that mixing is a fairly simple process. Mixing can be complicated by factors such solution viscosity, sample/component volumes, and the requirement to mix on a rapid time frame. In a regular T- or Y-flow cell there is often "laminar flow," a flow in which the two sides do not mix. The purpose of this experiment is to design and fabricate an "efficient" mixing flow cell. Blue and yellow dyed RO water will be used to evaluate laminar/non-laminar flow in the flow cell designs. These flow cells will be designed in TinkerCAD to make a virtual object, 3D printed, and cast with silicon. To facilitate flow, the silicon cast will be "sandwiched" between acrylic plates drilled and tapped to accommodate a luer fitting.		Chemist do a lot of mixing and it is generally assumed that mixing is a fairly simple process. Mixing can be complicated by factors such solution viscosity, sample/component volumes, and the requirement to mix on a rapid time frame. In a regular T- or Y-flow cell there is often "laminar flow," a flow in which the two sides do not mix. The purpose of this experiment is to design and fabricate an "efficient" mixing flow cell. Blue and yellow dyed RO water will be used to evaluate laminar/non-laminar flow in the flow cell designs. These flow cells will be designed in TinkerCAD to make a virtual object, 3D printed, and cast with silicon. To facilitate flow, the silicon cast will be "sandwiched" between acrylic plates drilled and tapped to accommodate a luer fitting.
		+
		+	The following video shows and interesting phenomenon related to mixing viscous fluids (Thanks Gene for the suggestion!)
		+
		+	https://youtu.be/UpJ-kGII074

	==Producing The Virtual Object==		==Producing The Virtual Object==

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 [[File:Yflow_cell.PNG|thumb|100px|frame|right|Typical Y-Flow Cell with Laminar Flow]]
-The purpose of this experiment is to see if distinguishing a flow cells mixing area would change the flow through the system. In a regular T- or Y-flow cell there is a laminar flow, a flow in which the two sides do not mix, that can be manipulated to give a non-laminar flow through the system. Using ten different manipulation of a typical flow cell, a way of having a non-laminar flow was experimented. Blue and yellow dyed RO water was used to determine the laminar/non-laminar flow of the flow cells by way of peristaltic flow pumps and gravity filtration. These flow cells were created using TinkerCAD to make a virtual object, 3D printed, and cast with silicon. To attach the pumps, either peristaltic or gravity, to the flow cell, an acrylic plastic was drilled and tapped at the precise measurements of the individual flow cells created. Each flow cell was used and it was determined which resulted in laminar flow and which resulted in non-laminar flow as shown below.
+Chemist do a lot of mixing and it is generally assumed that mixing is a fairly simple process. Mixing can be complicated by factors such solution viscosity, sample/component volumes, and the requirement to mix on a rapid time frame. In a regular T- or Y-flow cell there is often "laminar flow," a flow in which the two sides do not mix. The purpose of this experiment is to design and fabricate an "efficient" mixing flow cell. Blue and yellow dyed RO water will be used to evaluate laminar/non-laminar flow in the flow cell designs. These flow cells will be designed in TinkerCAD to make a virtual object, 3D printed, and cast with silicon. To facilitate flow, the silicon cast will be "sandwiched" between acrylic plates drilled and tapped to accommodate a luer fitting.
 ==Producing The Virtual Object==