Final Research - Revision history

Justin2.1.0 at 16:40, 15 December 2021

2021-12-15T16:40:27Z

Justin2.1.0 at 08:59, 15 December 2021

2021-12-15T08:59:08Z

Justin2.1.0 at 08:58, 15 December 2021

2021-12-15T08:58:20Z

Justin2.1.0 at 08:54, 15 December 2021

2021-12-15T08:54:59Z

Justin2.1.0 at 08:52, 15 December 2021

2021-12-15T08:52:06Z

Justin2.1.0 at 08:51, 15 December 2021

2021-12-15T08:51:46Z

Justin2.1.0 at 08:50, 15 December 2021

2021-12-15T08:50:39Z

Justin2.1.0 at 08:46, 15 December 2021

2021-12-15T08:46:50Z

Justin2.1.0 at 08:45, 15 December 2021

2021-12-15T08:45:52Z

Justin2.1.0 at 08:44, 15 December 2021

2021-12-15T08:44:15Z

← Older revision		Revision as of 16:40, 15 December 2021
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	The HPLC data was not as expected seeing that only one maxima showed up properly in the graph. This might have been from one of the dyes that since the HPLC data was taken on a different day than the UV/Vis spectra. In testing for the future, all samples must be tested on the same day for better results graphically and to keep conditions constant.		The HPLC data was not as expected seeing that only one maxima showed up properly in the graph. This might have been from one of the dyes that since the HPLC data was taken on a different day than the UV/Vis spectra. In testing for the future, all samples must be tested on the same day for better results graphically and to keep conditions constant.
		+
		+
		+	==References==
		+
		+	[US Food and Drug Administration. Safety. FD&C Blue No. 1 (Blue 1) in enteral feeding solutions. Accessed 10/19/2011. http://www.fda.gov/Safety/MedWatch/SafetyInformation/SafetyAlertsforHumanMedicalProducts/ucm169530.htm
		+
		+	U.S. Food and Drug Administration. Color Additives: FDA's Regulatory Process and Historical Perspectives. Accessed 10/20/2011. http://www.fda.gov/ForIndustry/ColorAdditives/RegulatoryProcessHistoricalPerspectives/default.htm
		+
		+	Kenneth D. Ross. ''Reduction of Azo food dyes FD&C Red 2 (Amaranth) FD&C Red 40 by Thermally Degraded d-Fructose and D-Glucose''. J. Agrie. Food Chem., Vol. 23, No. 3. 1975

← Older revision		Revision as of 08:59, 15 December 2021
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	[[File:AR.png\|400px]]		[[File:AR.png\|400px]]

		+	Red 40

		+	[[File:bluebrilli.png\|400px]]
		+
		+	Blue 1

	==Experimental Methods==		==Experimental Methods==

← Older revision		Revision as of 08:58, 15 December 2021
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	Food dyes, synthesized originally from coal tar and now petroleum, have long been controversial. Many dyes have been banned because of their adverse effects on laboratory animals. Therefore, these food dyes might be hazardous in certain quantities to people, so it is imperative that these food dyes made by companies are always researched upon and analyzed to make sure that they are being consumed at the right concentrations inside foods that they are mixed with. Using the UV-Vis and the HPLC, analysis of food dyes and discovery of their components at particular concentrations becomes possible. Brilliant blue commonly known as food blue 1 or FD and C blue 1 is a synthetic blue dye that is used in foods, drugs and cosmetics and many more. Red 40 also known as Allura Red is a monoazo dye that is used widely in a range of food products and drinks to display different shades of red in the foods they are in. Blue 1, Red 40, Yellow 5, and Yellow 6 have long been known to cause allergic reactions in some people. CSPI says that while those reactions are not common, they can be serious and provide reason enough to ban those dyes. Furthermore, numerous studies have demonstrated that dyes cause hyperactivity in children. Being able to analyze the dye through the HPLC and the UV-Vis is therefore important and would be able to quantify its abundance in the consumable products that is found around us.		Food dyes, synthesized originally from coal tar and now petroleum, have long been controversial. Many dyes have been banned because of their adverse effects on laboratory animals. Therefore, these food dyes might be hazardous in certain quantities to people, so it is imperative that these food dyes made by companies are always researched upon and analyzed to make sure that they are being consumed at the right concentrations inside foods that they are mixed with. Using the UV-Vis and the HPLC, analysis of food dyes and discovery of their components at particular concentrations becomes possible. Brilliant blue commonly known as food blue 1 or FD and C blue 1 is a synthetic blue dye that is used in foods, drugs and cosmetics and many more. Red 40 also known as Allura Red is a monoazo dye that is used widely in a range of food products and drinks to display different shades of red in the foods they are in. Blue 1, Red 40, Yellow 5, and Yellow 6 have long been known to cause allergic reactions in some people. CSPI says that while those reactions are not common, they can be serious and provide reason enough to ban those dyes. Furthermore, numerous studies have demonstrated that dyes cause hyperactivity in children. Being able to analyze the dye through the HPLC and the UV-Vis is therefore important and would be able to quantify its abundance in the consumable products that is found around us.
		+
		+	[[File:AR.png\|400px]]
		+
		+

	==Experimental Methods==		==Experimental Methods==

@@ Line 53: / Line 53: @@
 [[File:Picture1.png|400px]]
 [[File:Picture2.png|400px]]
 [[File:Picture3.png|400px]]
 [[File:Picture4.png|400px]]
 [[File:Picture5.png|400px]]
 [[File:Picture6.png|400px]]

← Older revision		Revision as of 08:52, 15 December 2021
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	[[File:Picture6.png\|400px]]		[[File:Picture6.png\|400px]]
		+

	Table I shows the data extracted from the graph in figure1 used to obtain the standard curve in figure 2 for Red 40. Table II also shows the data extracted from the graph in figure 3 used to make standard curve graph in figure 4. Although as seen in the graph that there are less points on both graphs for Red 40 and Blue 1, these were taken off to make an appropriate standard curve as measured absorption maxima above 1.5 in the UV/Vis would give a less accurate data in calculation for molar absorptivity. Figure 5 shows absorption maxima for the Gatorade drink and the first absorption maxima in that graph represents Red 40 appearing at around 415nm and the second maxima represents that of Blue 1 appearing at around 625nm. These graphs are seen to match the standards from the sample mixtures in previous graph figures showing that these compounds or components are actually present in the Gatorade drink. For better results, all of these samples were measured on the same day. Figure 6 shows the HPLC data collected for Gatorade purple drink and it is seen that only one component of the drink eluted. There is also a second line that cuts through the which could be that of the second dye but might not have been detected properly on the HPLC.		Table I shows the data extracted from the graph in figure1 used to obtain the standard curve in figure 2 for Red 40. Table II also shows the data extracted from the graph in figure 3 used to make standard curve graph in figure 4. Although as seen in the graph that there are less points on both graphs for Red 40 and Blue 1, these were taken off to make an appropriate standard curve as measured absorption maxima above 1.5 in the UV/Vis would give a less accurate data in calculation for molar absorptivity. Figure 5 shows absorption maxima for the Gatorade drink and the first absorption maxima in that graph represents Red 40 appearing at around 415nm and the second maxima represents that of Blue 1 appearing at around 625nm. These graphs are seen to match the standards from the sample mixtures in previous graph figures showing that these compounds or components are actually present in the Gatorade drink. For better results, all of these samples were measured on the same day. Figure 6 shows the HPLC data collected for Gatorade purple drink and it is seen that only one component of the drink eluted. There is also a second line that cuts through the which could be that of the second dye but might not have been detected properly on the HPLC.

← Older revision		Revision as of 08:51, 15 December 2021
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	[[File:Picture6.png\|400px]]		[[File:Picture6.png\|400px]]
		+
		+	Table I shows the data extracted from the graph in figure1 used to obtain the standard curve in figure 2 for Red 40. Table II also shows the data extracted from the graph in figure 3 used to make standard curve graph in figure 4. Although as seen in the graph that there are less points on both graphs for Red 40 and Blue 1, these were taken off to make an appropriate standard curve as measured absorption maxima above 1.5 in the UV/Vis would give a less accurate data in calculation for molar absorptivity. Figure 5 shows absorption maxima for the Gatorade drink and the first absorption maxima in that graph represents Red 40 appearing at around 415nm and the second maxima represents that of Blue 1 appearing at around 625nm. These graphs are seen to match the standards from the sample mixtures in previous graph figures showing that these compounds or components are actually present in the Gatorade drink. For better results, all of these samples were measured on the same day. Figure 6 shows the HPLC data collected for Gatorade purple drink and it is seen that only one component of the drink eluted. There is also a second line that cuts through the which could be that of the second dye but might not have been detected properly on the HPLC.
		+
		+
		+
		+	==Summary==
		+
		+	The HPLC data was not as expected seeing that only one maxima showed up properly in the graph. This might have been from one of the dyes that since the HPLC data was taken on a different day than the UV/Vis spectra. In testing for the future, all samples must be tested on the same day for better results graphically and to keep conditions constant.

← Older revision		Revision as of 08:50, 15 December 2021
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	[[File:Picture1.png\|400px]]		[[File:Picture1.png\|400px]]
		+
		+	[[File:Picture2.png\|400px]]
		+
		+	[[File:Picture3.png\|400px]]
		+
		+	[[File:Picture4.png\|400px]]
		+
		+	[[File:Picture5.png\|400px]]
		+
		+	[[File:Picture6.png\|400px]]

← Older revision		Revision as of 08:46, 15 December 2021
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	\|}		\|}

−	~~<gallery>~~	+	[[File:Picture1.png\|400px]]
−	~~Example~~.~~jpg~~\|~~Caption1~~
−	~~Example.jpg\|Caption2~~
−	~~</gallery>~~

← Older revision		Revision as of 08:45, 15 December 2021
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	\| Dilution 5 \|\| 0.000005124 \|\| 0.1558		\| Dilution 5 \|\| 0.000005124 \|\| 0.1558
	\|}		\|}
		+
		+	<gallery>
		+	Example.jpg\|Caption1
		+	Example.jpg\|Caption2
		+	</gallery>

@@ Line 22: / Line 22: @@
 |  || Concentration || Absoption
 |-
-| Stock || Example || Example
+| Stock || 0.00225615 || 3.0721
 |-
-| Dilution 1 || 0.00225615 || 3.0721
+| Dilution 1 || 0.0011282 || 1.1509
 |-
-| Dilution 2 || 0.0011282 || 1.1509
+| Dilution 2 || 0.00056405 || 0.53712
 |-
-| Dilution 3 || 0.00056405 || 0.53712
+| Dilution 3 || 0.000282025 || 0.24504
 |}