Caffeine HPLC Method - Revision history

Bes at 13:04, 6 October 2016

2016-10-06T13:04:24Z

2016-10-06T13:03:20Z

2016-04-19T15:48:30Z

Solvents

2016-04-19T15:48:19Z

UV Detection

2016-04-19T15:47:49Z

Pump Gradient

2016-04-19T15:47:25Z

High Pressure Liquid Chromatography Setup (HPLC)

2016-04-19T15:40:07Z

2016-04-19T15:39:16Z

High Pressure Liquid Chromatography Setup (HPLC)

2016-04-19T15:38:57Z

High Pressure Liquid Chromatography Setup (HPLC)

2016-04-19T15:38:46Z

Trial Configuration

@@ Line 7: / Line 7: @@
 *Mobile Phase
 **Acetonitrile
-**0.01% Acidified Water
+**0.1% Acidified Water
 Samples should be filtered through 45 μm syringe filters into HPLC sample vials, capped, labeled, and loaded into the HPLC auto-sampler carousel.

← Older revision		Revision as of 13:03, 6 October 2016
Line 1:		Line 1:
−	Caffeine can be detected using Reversed Phase High Pressure Liquid Chromatography with UV Detection.	+	Caffeine can be detected using Reversed Phase High Pressure Liquid Chromatography with UV Detection. (Chris Knutson, 2015)

	In order to accomplish caffeine analysis the HPLC system requires the following:		In order to accomplish caffeine analysis the HPLC system requires the following:

@@ Line 63: / Line 63: @@
 ==Solvents==
-Solvent A: Acetonitrile
+*Solvent A: Acetonitrile
-Solvent B: 0.01% Acidified Water
+*Solvent B: 0.01% Acidified Water
 ==UV Detection==

@@ Line 67: / Line 67: @@
 ==UV Detection==
-Dual Wavelength Detection
+*Dual Wavelength Detection
-Channel 1: 275nm
+*Channel 1: 275nm
-Channel 2: 325nm
+*Channel 2: 325nm

@@ Line 18: / Line 18: @@
 ==Pump Gradient==
-{|
+{| class="wikitable"
 ||
 ||Time

← Older revision		Revision as of 15:47, 19 April 2016
Line 14:		Line 14:

	=High Pressure Liquid Chromatography Setup (HPLC)=		=High Pressure Liquid Chromatography Setup (HPLC)=
		+
		+
		+	==Pump Gradient==
		+
		+	{\|
		+	\|\|
		+	\|\|Time
		+	\|\|Flow
		+	\|\|%A
		+	\|\|%B
		+	\|\|Curve
		+	\|-
		+	\|\|1
		+	\|\|
		+	\|\|1.00
		+	\|\|0.0
		+	\|\|100.0
		+	\|\|
		+	\|-
		+	\|\|2
		+	\|\|3.00
		+	\|\|1.00
		+	\|\|0.0
		+	\|\|100.0
		+	\|\|6
		+	\|-
		+	\|\|3
		+	\|\|15.00
		+	\|\|1.00
		+	\|\|60.0
		+	\|\|40.0
		+	\|\|6
		+	\|-
		+	\|\|4
		+	\|\|20.00
		+	\|\|1.00
		+	\|\|60.0
		+	\|\|40.0
		+	\|\|6
		+	\|-
		+	\|\|5
		+	\|\|25.00
		+	\|\|1.00
		+	\|\|0.0
		+	\|\|100.0
		+	\|\|6
		+	\|}
		+
		+	==Solvents==
		+	Solvent A: Acetonitrile
		+	Solvent B: 0.01% Acidified Water
		+
		+	==UV Detection==
		+	Dual Wavelength Detection
		+	Channel 1: 275nm
		+	Channel 2: 325nm
		+
		+

	{\|		{\|

← Older revision		Revision as of 15:40, 19 April 2016
Line 9:		Line 9:
	**0.01% Acidified Water		**0.01% Acidified Water

−	Samples should be filtered through 45 μm syringe filters into HPLC sample vials and loaded into the HPLC auto-sampler carousel.	+	Samples should be filtered through 45 μm syringe filters into HPLC sample vials, capped, labeled, and loaded into the HPLC auto-sampler carousel.

@@ Line 15: / Line 15: @@
 =High Pressure Liquid Chromatography Setup (HPLC)=
-{|align="left"
+{|
 ||[[File:1.PNG|thumb|250px|frame|center|HPLC Pump Gradient Flow Settings]]
 ||[[File:2.PNG|thumb|250px|frame|center|HPLC Pump Solvent Settings]]

@@ Line 27: / Line 27: @@
 =Trial Configuration=
-the samples can be injected in 10 μl increments and trials can range in length from 15-30 minutes depending upon the complexity of one's sample.
+The samples can be injected in 10 μl increments and trials can range in length from 15-30 minutes depending upon the complexity of one's sample.
 =Results=