Bradley Dulee BIOC430 S17 - Revision history

Bdulee: /* References (2 minimum) */

2017-01-24T19:34:52Z

References (2 minimum)

Bdulee: /* Research Times */

2017-01-24T19:34:21Z

Research Times

Bdulee at 19:33, 24 January 2017

2017-01-24T19:33:50Z

Bdulee: Created page with "Chemistry/Biochemistry Research 430 :Fall 2016 :Brad Dulee :Senior Biochemistry Major ==Research Times== TBD - 4 hours per week : section 01 = 0.25 credit = 4 hours per week...."

2017-01-24T19:31:43Z

Created page with "Chemistry/Biochemistry Research 430 :Fall 2016 :Brad Dulee :Senior Biochemistry Major ==Research Times== TBD - 4 hours per week : section 01 = 0.25 credit = 4 hours per week...."

New page

Chemistry/Biochemistry Research 430
:Fall 2016
:Brad Dulee
:Senior Biochemistry Major

==Research Times==
TBD - 4 hours per week
: section 01 = 0.25 credit = 4 hours per week.

==Proposed Research Project==
===Increasing Growth-Inhibitory Compound Tolerance in ''Escherichia coli'' For Use in Biofuel Production===

===General Information===
:Advisor: Laura Moore
:Other research student collaborators: Tyler Bailey

===Proposal===
It is the goal of this project to focus on increasing the tolerance of ''Escherichia coli'' against the harmful byproducts of lignocellulosic biomass pretreatment methods for use in biofuel production.
===Instruments to be used===
:- I2400 incubator shaker (37°C)
:- Allegra 25R Centrifuge (4°C)
:- Bio Rad MicroPulser Electroporator
:- Multiwell FC Microwell Plate Reader
:- Nanodrop

===References (2 minimum)===
1. Mills, T. Y.; Sandoval, N. R.; Gill, R. T. Cellulosic hydrolysate toxicity and tolerance mechanisms in "Escherichia coli". "Biotechnology for Biofuels" 2009, 2:26.

2. Wang, X.; Miller, E. N.; Yomano, L. P.; Zhang, X.; Shanmugam, K. T.; Ingram, L. O. Increased Furfural Tolerance Due to Overexpression of NADH-Dependent Oxidoreductase FucO in "Escherichia coli" Strains Engineered for the Production of Ethanol and Lactate. "Applied and Environment Biology" 2011, 77:15, 5132-5140.

3. Richardson,T. L.; Harner, N. K.; Bajwa, P. K.; Trevors, J. T.; Lee, H. Approaches to Deal with Toxic Inhibitors During Fermentation of Lignocellulosic Substrates. "ACS Symposium Series". 2011, 1067, Chapter 7, 171-202.

4. Olsson, L.; Hahn-Hägerdal, B.; Fermentation of Lignocellulosic Hydrosylates for Ethanol Production. "Enzyme and Microbial Technology". 1996, 18, 312-331.

5. Wang, X.; Miller, E. N.; Yomano, L. P.; Zhang, X.; Shanmugam, K. T.; Ingram, L. O. Increased Furan Tolerance in "Escherichia Coli" Due to a Cryptic ucpA Gene. "Applied and Environmental Microbiology". 2012, 78:7, 2452-2455.

6. Weil, J. R.; Dien, B.; Bothast, R.; Hendrickson, R.; Mosier, N. S.; Ladisch, M. R. Removal of Fermentation Inhibitors Formed during Pretreatment of Biomass by Polymeric Adsorbents. "Ind. Eng. Chem." Res. 2002, 41:24, 6132-6138.

===Research pledge===
I, Brad Dulee, have read the Chem/Bioc 430 course syllabus and understand the general structure and expectations of the research program. The above material was prepared after consultation, and in conjunction with my research advisor.

@@ Line 5: / Line 5: @@
 ==Research Times==
-M-F - 8 hours per week
+TBD - 8 hours per week
 : section 01 = 0.50 credit = 8 hours per week.

@@ Line 24: / Line 24: @@
 :- Nanodrop
-===References (2 minimum)===
+===References===
 .	Mills, T. Y.; Sandoval, N. R.; Gill, R. T. Cellulosic hydrolysate toxicity and tolerance mechanisms in "Escherichia coli". "Biotechnology for Biofuels" 2009, 2:26.

@@ Line 5: / Line 5: @@
 ==Research Times==
-TBD - 4 hours per week
+M-F - 8 hours per week
-: section 01 = 0.25 credit = 4 hours per week.
+: section 01 = 0.50 credit = 8 hours per week.
 ==Proposed Research Project==
@@ Line 13: / Line 13: @@
 ===General Information===
 :Advisor: Laura Moore
-:Other research student collaborators: Tyler Bailey
+:Previous research student collaborators: Tyler Bailey
 ===Proposal===