Benjamin Stillwell Chem430 F16 - Revision history

Benstillwell: /* References (2 minimum) */

2016-09-06T16:29:54Z

References (2 minimum)

Benstillwell: /* General Information */

2016-09-06T16:16:49Z

General Information

Benstillwell: /* General Information */

2016-09-06T16:13:21Z

General Information

Benstillwell: /* References (2 minimum) */

2016-09-06T16:07:34Z

References (2 minimum)

Benstillwell: /* References (2 minimum) */

2016-09-06T16:07:10Z

References (2 minimum)

Benstillwell: /* Project Title */

2016-09-06T15:51:30Z

Project Title

Benstillwell: /* Proposal */

2016-09-06T15:50:55Z

Proposal

Benstillwell at 01:13, 2 September 2016

2016-09-02T01:13:27Z

Benstillwell at 01:11, 2 September 2016

2016-09-02T01:11:25Z

Benstillwell: Created page with "Chemistry/Biochemistry Research 430 :Fall 2016 :Ian Salveson :Senior Chemistry Major ==Research Times== T/TH 10am-12pm ==Proposed Research Project== Investigating the metab..."

2016-09-02T01:10:48Z

Created page with "Chemistry/Biochemistry Research 430 :Fall 2016 :Ian Salveson :Senior Chemistry Major ==Research Times== T/TH 10am-12pm ==Proposed Research Project== Investigating the metab..."

New page

Chemistry/Biochemistry Research 430
:Fall 2016
:Ian Salveson
:Senior Chemistry Major

==Research Times==
T/TH 10am-12pm

==Proposed Research Project==
Investigating the metabolism of N-acetyl L-tyrosine modeled by an enzymatic, single-electron oxidation using HRP.

===Project Title===

===General Information===
:Advisor: Dr. Bradley E. Sturgeon

===Proposal===
Synthesize the primary products of this oxidation and investigate their activity in a similar system to determine the level to which N-acetyl L-tyrosine is oxidized by this type of enzyme.

===Instruments to be used===
HPLC, Flash Chromotography System, NMR, Immobilized Enzyme column, ESR
===References (2 minimum)===
1.
2.

===Research pledge===
I, Ben Stillwell, have read the Chem/Bioc 430 course syllabus and understand the general structure and expectations of the research program. The above material was prepared after consultation, and in conjunction with my research advisor.

← Older revision		Revision as of 16:16, 6 September 2016
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	Other Research Collaborators: [[Nadia Ayala\|Nadia Ayala]] and [[Ian C Salveson\|Ian C Salveson]]		Other Research Collaborators: [[Nadia Ayala\|Nadia Ayala]] and [[Ian C Salveson\|Ian C Salveson]]
		+
		+	Previous/On-Going Research: [[N-Acetyl L-Tyrosine\|N-Acetyl L-Tyrosine]]

	===Proposal===		===Proposal===

@@ Line 23: / Line 23: @@
 HPLC, Flash Chromotography System, NMR, Immobilized Enzyme column, ESR
 ===References (2 minimum)===
-.Sturgeon, B.E.; Chen, Y.R.; Mason, R.P. (2003) Analytical Chemistry. 75(19), 5006-5011.
+.Sturgeon, B.E.; Chen, Y.R.; Mason, R.P. (2003) ''Analytical Chemistry''. 75(19), 5006-5011.
-.Sturgeon, B.E., Glover, R.E., Chen, Y.R., Burka, L.T., and Mason, R.P. (2001) J. Biol. Chem. 276, 45516-45521.
+.Sturgeon, B.E., Glover, R.E., Chen, Y.R., Burka, L.T., and Mason, R.P. (2001) ''J. Biol. Chem''. 276, 45516-45521.
 ===Research pledge===
 I, Ben Stillwell, have read the Chem/Bioc 430 course syllabus and understand the general structure and expectations of the research program. The above material was prepared after consultation, and in conjunction with my research advisor.

@@ Line 16: / Line 16: @@
 ===General Information===
 Advisor: Dr. Bradley E. Sturgeon
 ===Proposal===

@@ Line 12: / Line 12: @@
 ===Project Title===
+Fate of Oxidation of N-Acetyl L-Tyrosine
 ===General Information===

@@ Line 18: / Line 18: @@
 ===Proposal===
-Synthesize the primary products of this oxidation and investigate their activity in a similar system to determine the level to which N-acetyl L-tyrosine is oxidized by this type of enzyme.
+Synthesize the primary products of this oxidation and investigate their activity in a similar system to determine the level to which N-acetyl L-tyrosine (NAT) is oxidized by this type of enzyme.  By continuing the research conducted previously in the Kieft Summer Research Program, the compound NAT will first be oxidized using HRP in a bio-reactor at a controlled rate of flow.  Next the compound will be separated using flash chromatography based on polarity.  The final step in investigating the fate of oxidation will be to determine the isolated products that are formed.  This will be done using NMR to identify the structure of the products.
 ===Instruments to be used===

@@ Line 1: / Line 1: @@
 Chemistry/Biochemistry Research 430
 :Fall 2016
-:Ian Salveson
+:Ben Stillwell
-:Senior Chemistry Major
+:Junior Biochemistry Major
 ==Research Times==