Benjamin Stillwell BIOC430 S17 - Revision history

Benstillwell: /* Research Times */

2017-04-18T14:01:45Z

Research Times

Benstillwell: /* Instruments to be used */

2017-01-30T16:09:07Z

Instruments to be used

Benstillwell: /* References */

2017-01-29T19:54:28Z

References

Benstillwell: /* General Information */

2017-01-29T19:54:01Z

General Information

Benstillwell at 19:53, 29 January 2017

2017-01-29T19:53:02Z

Benstillwell: /* Research Times */

2017-01-29T19:52:36Z

Research Times

Benstillwell: /* Proposed Research Project */

2017-01-29T19:49:22Z

Proposed Research Project

Benstillwell: /* Research Times */

2017-01-29T19:38:34Z

Research Times

Benstillwell: Created page with "Chemistry/Biochemistry Research 430 :Fall 2016 :Ben Stillwell :Junior Biochemistry Major ==Research Times== T/TH 10am-12pm ==Proposed Research Project== Investigating the m..."

2017-01-29T19:38:12Z

Created page with "Chemistry/Biochemistry Research 430 :Fall 2016 :Ben Stillwell :Junior Biochemistry Major ==Research Times== T/TH 10am-12pm ==Proposed Research Project== Investigating the m..."

New page

Chemistry/Biochemistry Research 430
:Fall 2016
:Ben Stillwell
:Junior Biochemistry Major

==Research Times==
T/TH 10am-12pm

==Proposed Research Project==
Investigating the metabolism of N-acetyl L-tyrosine modeled by an enzymatic, single-electron oxidation using HRP.

===Project Title===
Fate of Oxidation of N-Acetyl L-Tyrosine

===General Information===
Advisor: Dr. Bradley E. Sturgeon

Other Research Collaborators: [[Nadia Ayala|Nadia Ayala]] and [[Ian C Salveson|Ian C Salveson]]

Previous/On-Going Research: [[N-Acetyl L-Tyrosine|N-Acetyl L-Tyrosine]]

===Proposal===
Synthesize the primary products of this oxidation and investigate their activity in a similar system to determine the level to which N-acetyl L-tyrosine (NAT) is oxidized by this type of enzyme. By continuing the research conducted previously in the Kieft Summer Research Program, the compound NAT will first be oxidized using HRP in a bio-reactor at a controlled rate of flow. Next the compound will be separated using flash chromatography based on polarity. The final step in investigating the fate of oxidation will be to determine the isolated products that are formed. This will be done using NMR to identify the structure of the products.

===Instruments to be used===
HPLC, Flash Chromotography System, NMR, Immobilized Enzyme column, ESR
===References===
1.Sturgeon, B.E.; Chen, Y.R.; Mason, R.P. (2003) ''Analytical Chemistry''. 75(19), 5006-5011.

2.Sturgeon, B.E., Glover, R.E., Chen, Y.R., Burka, L.T., and Mason, R.P. (2001) ''J. Biol. Chem''. 276, 45516-45521.

===Research pledge===
I, Ben Stillwell, have read the Chem/Bioc 430 course syllabus and understand the general structure and expectations of the research program. The above material was prepared after consultation, and in conjunction with my research advisor.

@@ Line 5: / Line 5: @@
 ==Research Times==
-Monday/Friday 10am-12pm
+Tuesday/Thursday 9 AM - 11 AM
 ==Proposed Research Project==

@@ Line 27: / Line 27: @@
 ===Instruments to be used===
-HPLC, Flash Chromotography System, NMR, ESR
+Steam Distillation, HPLC, NMR, ESR
 ===References===
 .	Regnault-Roger, C. et al. (2012) Essential oils in insect control: low-risk products in a high-stakes world. Annu. Rev. Entomol. 57, 405-424.

@@ Line 5: / Line 5: @@
 ==Research Times==
-Monday 10am-12pm
+Monday/Friday 10am-12pm
 ==Proposed Research Project==

@@ Line 34: / Line 34: @@
 .	Tucker, A. O., & Debaggio, T. (2000) The Big Book of Herbs. Loveland, CO: Interweave Press.
 ===Research pledge===
 I, Ben Stillwell, have read the Chem/Bioc 430 course syllabus and understand the general structure and expectations of the research program. The above material was prepared after consultation, and in conjunction with my research advisor.

@@ Line 13: / Line 13: @@
 Characterization and Application of Essential Oils
 ===General Information===
-Advisor: Dr. Bradley E. Sturgeon
+Adviser: Dr. Bradley E. Sturgeon
 Other Research Collaborators: [[Nadia Ayala|Nadia Ayala]] and [[Ian C Salveson|Ian C Salveson]]
-Previous/On-Going Research:  [[N-Acetyl L-Tyrosine|N-Acetyl L-Tyrosine]]
+Previous Research:  [[N-Acetyl L-Tyrosine|N-Acetyl L-Tyrosine]]
 ===Proposal===

@@ Line 1: / Line 1: @@
 Chemistry/Biochemistry Research 430
-:Fall 2016
+:Spring 2017
 :Ben Stillwell
 :Junior Biochemistry Major

@@ Line 9: / Line 9: @@
 ==Proposed Research Project==
-Investigating the metabolism of N-acetyl L-tyrosine modeled by an enzymatic, single-electron oxidation using HRP.
+Extraction, characterization, and application of essential oils with the goal of exploring the anti-pest, anti-fungal, and anti-bacterial properties of the oils.  The characterization of the oils will be done using GC-MS, NMR, and EPR.
 ===Project Title===
-Fate of Oxidation of N-Acetyl L-Tyrosine
+Characterization and Application of Essential Oils
 ===General Information===
 Advisor: Dr. Bradley E. Sturgeon
@@ Line 22: / Line 21: @@
 ===Proposal===
-Synthesize the primary products of this oxidation and investigate their activity in a similar system to determine the level to which N-acetyl L-tyrosine (NAT) is oxidized by this type of enzyme.  By continuing the research conducted previously in the Kieft Summer Research Program, the compound NAT will first be oxidized using HRP in a bio-reactor at a controlled rate of flow.  Next the compound will be separated using flash chromatography based on polarity.  The final step in investigating the fate of oxidation will be to determine the isolated products that are formed.  This will be done using NMR to identify the structure of the products.
+Essential oils are synthesized via secondary metabolic pathways and have a role in plant defenses against pathogens and herbivores, in plant reproduction, attraction of pollinators, and in thermotolerance.  Essential oils can be classified into two different groups based on their metabolic synthesis: terpenoids, which are monoterpenes and sesquiterpenes; and low molecular weight phenylpropanoids.
 ===Instruments to be used===
-HPLC, Flash Chromotography System, NMR, Immobilized Enzyme column, ESR
+HPLC, Flash Chromotography System, NMR, ESR
 ===References===
-.Sturgeon, B.E.; Chen, Y.R.; Mason, R.P. (2003) ''Analytical Chemistry''. 75(19), 5006-5011.
+.	Regnault-Roger, C. et al. (2012) Essential oils in insect control: low-risk products in a high-stakes world. Annu. Rev. Entomol. 57, 405-424.
-−
-.Sturgeon, B.E., Glover, R.E., Chen, Y.R., Burka, L.T., and Mason, R.P. (2001) ''J. Biol. Chem''. 276, 45516-45521.
+.	Pavela, R and Benelli, G. (2016) Essential Oils as Ecofriendly Biopesticides?  Challenges and Constraints. Trends in Plant Science. 21, 1000-1007.
 ===Research pledge===
 I, Ben Stillwell, have read the Chem/Bioc 430 course syllabus and understand the general structure and expectations of the research program. The above material was prepared after consultation, and in conjunction with my research advisor.