Anaerobic Chamber for Cell Cultivation - Revision history

Scroslow at 13:36, 4 August 2019

2019-08-04T13:36:50Z

Scroslow at 13:33, 4 August 2019

2019-08-04T13:33:51Z

Scroslow at 13:32, 4 August 2019

2019-08-04T13:32:59Z

Scroslow: /* Amount of CO2 Needed per Trial */

2019-08-04T13:30:48Z

Amount of CO2 Needed per Trial

Scroslow: /* Sample Calculations */

2019-08-04T13:30:18Z

Sample Calculations

Scroslow: /* Construction */

2019-08-04T13:26:12Z

Construction

Scroslow: /* Personal Information */

2019-08-04T13:25:55Z

Personal Information

Scroslow at 13:25, 4 August 2019

2019-08-04T13:25:11Z

Scroslow: Created page with "=Background= Gut bacteria grow under anaerobic conditions inside the body; however, we do not have the proper equipment to effectively mimic the environment of a human gut at..."

2019-08-04T13:23:46Z

Created page with "=Background= Gut bacteria grow under anaerobic conditions inside the body; however, we do not have the proper equipment to effectively mimic the environment of a human gut at..."

New page

=Background=
Gut bacteria grow under anaerobic conditions inside the body; however, we do not have the proper equipment to effectively mimic the environment of a human gut at this time. Currently, we grow this bacteria in the incubator shaker and the plate reader, but this can only be done in an aerobic environment. Ideally, the bacteria would be grown and experimented upon in an anaerobic chamber (otherwise known as a glove box). Therefore, we would like to build our own version of a glove box to provide the necessary environment to best facilitate the growth of common types of gut bacteria for experimentation.

=Construction=
The construction of the anaerobic chamber will be simple and will only require a few components listed below (Note: an X represents that we currently have the item):
*Plastic tote
*Power supply (specs TBD) X?
*Arduino Uno X
*Peltier Module X
*Heat Sink X
*Transistor X
*LCD Screen X
*Rotary Potentiometer X
*Temperature Sensor X
*Foam/sealer(caulk?)
*Valves (2x)
*Estimated Cost: $50

The setup is simple, the plastic tote will be sealed using the foam and cutouts will be made for the peltier module and temperature sensor. The peltier module and fan will be used to regulate the temperature and will be attached to the side of tote. The Arduino, power supply, and LCD screen will be housed in a separate container (made via 3D printing). A simple circuit will be made to power and control all of the peripherals and software will be written using Arduino IDE.

=Usage/Methodology=
Once finished, the plate reader can be placed into the tote as well as some dry ice. Once the dry ice sublimates, it will displace the air within the tote which will then escape through a valve on the top of the tote. Then, nitrogen will be filled into the container from a valve in the top to remove the CO2 and thus produce the anaerobic conditions necessary. The peltier module will then be turned on and the temperature will be increased until 37℃ is reached; this temperature will then be held constant.

=Sample Calculations=
''Amount of CO2 Needed per Trial''
*Assuming a 64 quart plastic container = ~60.6 liters
*Displace 1.5x volume to account for CO2 loss from top = ~90 liters
*Assuming STP conditions and 1 mole of CO2 = 22.4 L
*Moles CO2: 90 / 22.4 = ~4 moles of dry ice
*Mass of CO2: 4 mol * 44.01 g/mol = 180g of dry ice = .04 lbs
*Assuming price of $2/lb = '''~$1 per trial'''

=Possible Circuit Design=
[[File:Screenshot (52).png|500px]]

@@ Line 1: / Line 1: @@
-[[File:anaerobic chamber.jpg|link=https://www.instructables.com/id/DIY-Anaerobic-Chamber-aka-glove-box/|thumb|400px|DIY Anaerobic Chamber from Instructables]]
+[[File:anaerobic chamber.jpg|link=https://www.instructables.com/id/DIY-Anaerobic-Chamber-aka-glove-box/|thumb|360px|left|DIY Anaerobic Chamber from Instructables]]
 =Personal Information=
 *'''Project Members''': Katie Richter and Seth Croslow

@@ Line 1: / Line 1: @@
-[[File:anaerobic chamber.jpg|thumb|400px|DIY Anaerobic Chamber from Instructables]]
+[[File:anaerobic chamber.jpg|link=https://www.instructables.com/id/DIY-Anaerobic-Chamber-aka-glove-box/|thumb|400px|DIY Anaerobic Chamber from Instructables]]
 =Personal Information=
 *'''Project Members''': Katie Richter and Seth Croslow

← Older revision		Revision as of 13:32, 4 August 2019
Line 1:		Line 1:
		+
		+	[[File:anaerobic chamber.jpg\|thumb\|400px\|DIY Anaerobic Chamber from Instructables]]
	=Personal Information=		=Personal Information=
	*'''Project Members''': Katie Richter and Seth Croslow		*'''Project Members''': Katie Richter and Seth Croslow

@@ Line 27: / Line 27: @@
 =Sample Calculations=
-==Amount of CO<sub>2</sub> Needed per Trial==
+===Amount of CO<sub>2</sub> Needed per Trial===
 *Assuming a 64 quart plastic container = ~60.6 liters
 *Displace 1.5x volume to account for CO<sub>2</sub> loss from top = ~90 liters

@@ Line 27: / Line 27: @@
 =Sample Calculations=
-''Amount of CO<sub>2</sub> Needed per Trial''
+==Amount of CO<sub>2</sub> Needed per Trial==
 *Assuming a 64 quart plastic container = ~60.6 liters
 *Displace 1.5x volume to account for CO<sub>2</sub> loss from top = ~90 liters

@@ Line 19: / Line 19: @@
 *Foam/sealer(caulk?)
 *Valves (2x)
-*Estimated Cost: $50
+*'''Estimated Cost: $50'''
 The setup is simple, the plastic tote will be sealed using the foam and cutouts will be made for the peltier module and temperature sensor. The peltier module and fan will be used to regulate the temperature and will be attached to the side of tote. The Arduino, power supply, and LCD screen will be housed in a separate container (made via 3D printing). A simple circuit will be made to power and control all of the peripherals and software will be written using Arduino IDE.

← Older revision		Revision as of 13:25, 4 August 2019
Line 1:		Line 1:
		+	=Personal Information=
		+	'''Project Members''': Katie Richter and Seth Croslow
		+
	=Background=		=Background=
	Gut bacteria grow under anaerobic conditions inside the body; however, we do not have the proper equipment to effectively mimic the environment of a human gut at this time. Currently, we grow this bacteria in the incubator shaker and the plate reader, but this can only be done in an aerobic environment. Ideally, the bacteria would be grown and experimented upon in an anaerobic chamber (otherwise known as a glove box). Therefore, we would like to build our own version of a glove box to provide the necessary environment to best facilitate the growth of common types of gut bacteria for experimentation.		Gut bacteria grow under anaerobic conditions inside the body; however, we do not have the proper equipment to effectively mimic the environment of a human gut at this time. Currently, we grow this bacteria in the incubator shaker and the plate reader, but this can only be done in an aerobic environment. Ideally, the bacteria would be grown and experimented upon in an anaerobic chamber (otherwise known as a glove box). Therefore, we would like to build our own version of a glove box to provide the necessary environment to best facilitate the growth of common types of gut bacteria for experimentation.