MC Chem Wiki - User contributions [en]

Flow Cell Lab Activity

2016-04-25T12:07:39Z

Sujith: /* A Guide to creating animated gif's */

==Introduction to Flow Cells==
[[File:Yflow_cell.PNG|thumb|100px|frame|right|Typical Y-Flow Cell with Laminar Flow]]

The purpose of this experiment was to see if distinguishing a flow cells mixing area would change the flow through the system. In a regular T- or Y-flow cell there is a laminar flow, a flow in which the two sides do not mix, that can be manipulated to give a non-laminar flow through the system. Using ten different manipulation of a typical flow cell, a way of having a non-laminar flow was experimented. Blue and yellow dyed RO water was used to determine the laminar/non-laminar flow of the flow cells by way of peristaltic flow pumps and gravity filtration. These flow cells were created using TinkerCAD to make a virtual object, 3D printed, and cast with silicon. To attach the pumps, either peristaltic or gravity, to the flow cell, an acrylic plastic was drilled and tapped at the precise measurements of the individual flow cells created. Each flow cell was used and it was determined which resulted in laminar flow and which resulted in non-laminar flow as shown below.

==Producing The Virtual Object==

===Getting Started with TinkerCAD===
* Go to the TinkerCAD website (https://www.tinkercad.com/)
* Create an account or Login to an existing account
* Click on Create a New Design to get started.
* There are tutorials available in TinkerCAD to get used to object placements and using different shapes. Also refer this link (https://www.tinkercad.com/quests/)

===Dimensions of the Mold===
* The Outer Box : 56 mm X 26 mm with a height of 7 mm with a 2 mm bottom
* Interior Channels : Channel height is 2 mm and the width of each channel is 2 mm

===Things to Note===
* Use cylinders for mixing chambers instead of spheres to avoid undercuts.
* For channels, ideally use boxes instead of half cylinders or cylinders.

===Exporting the file===
Once the design is complete. Click the "Design" tab located to the top left corner of the screen and export the file as .STL

{|align="center"
|
||[[File:Tinker1.png|thumb|400px|right|Box Dimensions]]
||[[File:Tinker2.png|thumb|400px|right|Exporting File]]
|}

==Prep for 3d Printing==

A gcode file is required in order to print an object on the 3D printer. This file can be created by exporting one's Tinkercad object as an STL. The STL file can be imported into Matter Control.

The gcode file contains settings specific to the 3D printer being used in production of the flow cell. As a result, one must ensure that the proper printer, material, and settings are chosen before exportation of the final gcode.
{|align="center"
|Rowspan="2"|[[File:Printer.PNG|200px|thumb|center|Select The Printer in Matter Control]]
||[[File:Settings_Layers.PNG |200px|thumb|right|Matter Control Layer Settings]]
||[[File:Settings_Infill.PNG|200px|thumb|right|Matter Control Infill Settings]]
||[[File:Settings_Raft.PNG |200px|thumb|right|Matter Control Raft Settings]]
|-
||[[File:Settings_Support.PNG|200px|thumb|right|Matter Control Support Settings]]
||[[File:Settings_Filament_Filament.PNG|200px|thumb|right|Matter Control Filament Settings]]
||[[File:Settings_Filament_Cooling.PNG|200px|thumb|right|Matter Control Cooling Settings]]
|}

==3d Printing==

==Silicon Casting==

After the 3D-printed cast has been made, now the actual flow cell can be created. We have used a 10:1 ratio of Slygard 184 (a silicon monomer) to curating agent, or about 5 grams Slygard 184 to 0.5 grams curating agent. After this is mixed thoroughly, we pour it into the cast and place it in a desiccator and place a vacuum on it to let the air bubbles come to the surface; once the vacuum is taken off, the bubbles will pop if left long enough.

[[File:Cast in desiccator.jpg|200px|thumb|center|Silicon mold in Desiccator]]

After this, we can leave the cast to curate, or let the silicon harden and shape to the cast. There were a few ways this was done. Some put their cast into the oven, but due to the low melting temperature of the acrylic plastic, they started to melt, making them unable to be reused. Others, however, left theirs out over the time span of a week to harden. A table of how they have been done is listed below.

[[File:Screen Shot 2016-04-07 at 4.01.54 PM.png|600px|thumb|center|Curing Times]]

==Flow Cell Construction==
After completing the casting, the mold was placed on two glass slides. This created a sandwich in which solution could pass through. Holes were drilled on one of the glass slide, corresponding to each model using a driller of size 2mm. Adapters between the slide holes and mold model were utilized to allow the solutions inside. Solutions were made using color dyes blue and yellow in 500 ml of water. A waste beaker was used to collect the mixed solutions.
Peristaltic pumps were used to pump the colored solutions into the flow cell.
The rate of pumping differed in every model.
===[[File:Brads Flow Cell.PNG|200px]]===
===[[File:Flow setup .PNG|700px]]===

==Flow Cell Experimentation==
{|class="wikitable"
!colspan="11"|Physical Chemistry 2 April 2016 Results
|-
|
|Brad
|Chris
|Morgan
|Tyler
|Ian
|Kayla
|Matt
|Priscilla
|Deysi
|Sujith
|-
|Model
|
|[[File:Chris_Tinker.PNG |200px]]
|[[File:Morgan%27sVirtual.PNG|100px]]
|[[File:Tylers Tinkercad.PNG|100px]]
|[[File:Ian's_Mixer_Thing.PNG|200px]]
|[[File:Kaylas 3D idea.PNG |100px]]
|[[File:TinkerCad.png|100px]]
|[[File:Pris's Tinker.png|200px]]
|[[File:Deysi model Flowc cell 2.JPG|150px]]
|[[File:SujFlowCell.PNG|200px]]
|-
|Printed Mold
|
|[[File:Chris_Printed.png|200px]]
|[[File:MReality.PNG |100px]]
|[[File:Tylers mould.PNG |100px]]
|
|[[File:Kaylas 3D reality.png |100px]]
||[[File:Flowcell Mold.jpg |100px]]
|[[File:Pris' Printed.png|200px]]
|[[File:Deysi printed IMG 7598-1-.JPG|200px]]
|
|-
|Result
|[[File:Brads Flow Cell.PNG|150px]]
|[[File:Done.png |200px]]
|[[File:Morgan%27sFlow.PNG |200px]]
|
|
|
|[[File:Mixer.gif|300px]]
|[[File:Pris' Flow cell.PNG|300px]]
|[[File:Deysi flow cell final.jpg|150px]]
|[[File:FlowSuj.gif]]
|}

== A Guide to creating animated gif's ==
# Record a video
# Use (http://makeagif.com/video-to-gif) to create the animated gif.
# Upload the desired file to the website
# Make sure to reset the start time and short time to have an "active" gif
# Click "Make a GIF" and you'll have your animated .gif file

Flow Cell Lab Activity

2016-04-25T12:01:28Z

Sujith:

Flow Cell Lab Activity

2016-04-08T13:26:52Z

Sujith: /* Silicon Casting */

==Introduction to Flow Cells==
[[File:Yflow_cell.PNG|thumb|100px|frame|right|Typical Y-Flow Cell with Laminar Flow]]

The purpose of this experiment was to see if distinguishing a flow cells mixing area would change the flow through the system. In a regular T- or Y-flow cell there is a laminar flow, a flow in which the two sides do not mix, that can be manipulated to give a non-laminar flow through the system. Using ten different manipulation of a typical flow cell, a way of having a non-laminar flow was experimented. Blue and yellow dyed RO water was used to determine the laminar/non-laminar flow of the flow cells by way of peristaltic flow pumps and gravity filtration. These flow cells were created using TinkerCAD to make a virtual object, 3D printed, and cast with silicon. To attach the pumps, either peristaltic or gravity, to the flow cell, an acrylic plastic was drilled and tapped at the precise measurements of the individual flow cells created. Each flow cell was used and it was determined which resulted in laminar flow and which resulted in non-laminar flow as shown below.

==Producing The Virtual Object==

===Getting Started with TinkerCAD===
* Go to the TinkerCAD website (https://www.tinkercad.com/)
* Create an account or Login to an existing account
* Click on Create a New Design to get started.
* There are tutorials available in TinkerCAD to get used to object placements and using different shapes. Also refer this link (https://www.tinkercad.com/quests/)

===Dimensions of the Mold===
* The Outer Box : 56 mm X 26 mm with a height of 7 mm with a 2 mm bottom
* Interior Channels : Channel height is 2 mm and the width of each channel is 2 mm

===Things to Note===
* Use cylinders for mixing chambers instead of spheres to avoid undercuts.
* For channels, ideally use boxes instead of half cylinders or cylinders.

===Exporting the file===
Once the design is complete. Click the "Design" tab located to the top left corner of the screen and export the file as .STL

{|align="center"
|
||[[File:Tinker1.png|thumb|400px|right|Box Dimensions]]
||[[File:Tinker2.png|thumb|400px|right|Exporting File]]
|}

==Prep for 3d Printing==

A gcode file is required in order to print an object on the 3D printer. This file can be created by exporting one's Tinkercad object as an STL. The STL file can be imported into Matter Control.

The gcode file contains settings specific to the 3D printer being used in production of the flow cell. As a result, one must ensure that the proper printer, material, and settings are chosen before exportation of the final gcode.
{|align="center"
|Rowspan="2"|[[File:Printer.PNG|200px|thumb|center|Select The Printer in Matter Control]]
||[[File:Settings_Layers.PNG |200px|thumb|right|Matter Control Layer Settings]]
||[[File:Settings_Infill.PNG|200px|thumb|right|Matter Control Infill Settings]]
||[[File:Settings_Raft.PNG |200px|thumb|right|Matter Control Raft Settings]]
|-
||[[File:Settings_Support.PNG|200px|thumb|right|Matter Control Support Settings]]
||[[File:Settings_Filament_Filament.PNG|200px|thumb|right|Matter Control Filament Settings]]
||[[File:Settings_Filament_Cooling.PNG|200px|thumb|right|Matter Control Cooling Settings]]
|}

==3d Printing==

==Silicon Casting==

After the 3D-printed cast has been made, now the actual flow cell can be created. We have used a 10:1 ratio of Slygard 184 (a silicon monomer) to curating agent, or about 5 grams Slygard 184 to 0.5 grams curating agent. After this is mixed thoroughly, we pour it into the cast and place it in a desiccator and place a vacuum on it to let the air bubbles come to the surface; once the vacuum is taken off, the bubbles will pop if left long enough.

[[File:Cast in desiccator.jpg|200px|thumb|center|Silicon mold in Desiccator]]

After this, we can leave the cast to curate, or let the silicon harden and shape to the cast. There were a few ways this was done. Some put their cast into the oven, but due to the low melting temperature of the acrylic plastic, they started to melt, making them unable to be reused. Others, however, left theirs out over the time span of a week to harden. A table of how they have been done is listed below.

[[File:Screen Shot 2016-04-07 at 4.01.54 PM.png|600px|thumb|center|Curing Times]]

==Flow Cell Construction==
[[File:Brads Flow Cell.PNG|200px]]

==Flow Cell Experimentation==
===Flow Cell Designs===

{|align="center"
| Brad ||Chris||Morgan||Tyler||Ian
|-
|1||[[File:Chris_Tinker.PNG |200px]][[File:Chris_Printed.png|200px]]||[[File:Morgan%27sVirtual.PNG|100px]][[File:MReality.PNG |100px]]||[[File:Tylers Tinkercad.PNG|100px]][[File:Tylers mould.PNG |100px]]||[[File:Ian's_Mixer_Thing.PNG|200px]]
|}

{|align="center"
| Kayla||Matt||Priscilla||Desyi||Sujith
|-
|[[File:Kaylas 3D idea.PNG |100px]][[File:Kaylas 3D reality.png |100px]]||[[File:TinkerCad.png|100px]]||8||9||[[File:SujFlowCell.PNG|200px]]
|}

===Camera/Video Results===
{|align="center"
|1||A High Laminar Flow Day||Non-Laminar Flow Found||4||5
|-
|1||[[File:Done.png |200px]]||[[File:Morgan%27sFlow.PNG |200px]]||4||5
|}

{|align="center"
|6||7||8||9||Non-Laminar Flow
|-
|6||7||8||9||[[File:FlowSuj.gif]]
|}

File:FlowSuj.gif

2016-04-08T05:29:39Z

Sujith: Sujith uploaded a new version of File:FlowSuj.gif

Flow Cell Lab Activity

2016-04-08T05:24:30Z

Sujith: /* Camera/Video Results */

==Introduction to Flow Cells==
[[File:Yflow_cell.PNG|thumb|100px|frame|right|Typical Y-Flow Cell with Laminar Flow]]

The purpose of this experiment was to see if distinguishing a flow cells mixing area would change the flow through the system. In a regular T- or Y-flow cell there is a laminar flow, a flow in which the two sides do not mix, that can be manipulated to give a non-laminar flow through the system. Using ten different manipulation of a typical flow cell, a way of having a non-laminar flow was experimented. Blue and yellow dyed RO water was used to determine the laminar/non-laminar flow of the flow cells by way of peristaltic flow pumps and gravity filtration. These flow cells were created using TinkerCAD to make a virtual object, 3D printed, and cast with silicon. To attach the pumps, either peristaltic or gravity, to the flow cell, an acrylic plastic was drilled and tapped at the precise measurements of the individual flow cells created. Each flow cell was used and it was determined which resulted in laminar flow and which resulted in non-laminar flow as shown below.

==Producing The Virtual Object==

===Getting Started with TinkerCAD===
* Go to the TinkerCAD website (https://www.tinkercad.com/)
* Create an account or Login to an existing account
* Click on Create a New Design to get started.
* There are tutorials available in TinkerCAD to get used to object placements and using different shapes. Also refer this link (https://www.tinkercad.com/quests/)

===Dimensions of the Mold===
* The Outer Box : 56 mm X 26 mm with a height of 7 mm with a 2 mm bottom
* Interior Channels : Channel height is 2 mm and the width of each channel is 2 mm

===Things to Note===
* Use cylinders for mixing chambers instead of spheres to avoid undercuts.
* For channels, ideally use boxes instead of half cylinders or cylinders.

===Exporting the file===
Once the design is complete. Click the "Design" tab located to the top left corner of the screen and export the file as .STL

{|align="center"
|
||[[File:Tinker1.png|thumb|400px|right|Box Dimensions]]
||[[File:Tinker2.png|thumb|400px|right|Exporting File]]
|}

==Prep for 3d Printing==

A gcode file is required in order to print an object on the 3D printer. This file can be created by exporting one's Tinkercad object as an STL. The STL file can be imported into Matter Control.

The gcode file contains settings specific to the 3D printer being used in production of the flow cell. As a result, one must ensure that the proper printer, material, and settings are chosen before exportation of the final gcode.
{|align="center"
|Rowspan="2"|[[File:Printer.PNG|200px|thumb|center|Select The Printer in Matter Control]]
||[[File:Settings_Layers.PNG |200px|thumb|right|Matter Control Layer Settings]]
||[[File:Settings_Infill.PNG|200px|thumb|right|Matter Control Infill Settings]]
||[[File:Settings_Raft.PNG |200px|thumb|right|Matter Control Raft Settings]]
|-
||[[File:Settings_Support.PNG|200px|thumb|right|Matter Control Support Settings]]
||[[File:Settings_Filament_Filament.PNG|200px|thumb|right|Matter Control Filament Settings]]
||[[File:Settings_Filament_Cooling.PNG|200px|thumb|right|Matter Control Cooling Settings]]
|}

==3d Printing==

==Silicon Casting==

After the 3D-printed cast has been made, now the actual flow cell can be created. We have used a 10:1 ratio of Slygard 184 (a silicon monomer) to curating agent, or about 5 grams Slygard 184 to 0.5 grams curating agent. After this is mixed thoroughly, we pour it into the cast and place it in a desiccator and place a vacuum on it to let the air bubbles come to the surface; once the vacuum is taken off, the bubbles will pop if left long enough.

[[File:Cast in desiccator.jpg|200px|thumb|center|Matter Control Layer Settings]]

After this, we can leave the cast to curate, or let the silicon harden and shape to the cast. There were a few ways this was done. Some put their cast into the oven, but due to the low melting temperature of the acrylic plastic, they started to melt, making them unable to be reused. Others, however, left theirs out over the time span of a week to harden. A table of how they have been done is listed below.

[[File:Screen Shot 2016-04-07 at 4.01.54 PM.png|600px|thumb|center|Matter Control Layer Settings]]

==Flow Cell Construction==
[[File:Brads Flow Cell.PNG|200px]]

==Flow Cell Experimentation==
===Flow Cell Designs===

{|align="center"
| Brad ||Chris||Morgan||Tyler||Ian
|-
|1||[[File:Chris_Tinker.PNG |200px]][[File:Chris_Printed.png|200px]]||[[File:Morgan%27sVirtual.PNG|100px]][[File:MReality.PNG |100px]]||[[File:Tylers Tinkercad.PNG|100px]][[File:Tylers mould.PNG |100px]]||[[File:Ian's_Mixer_Thing.PNG|200px]]
|}

{|align="center"
| Kayla||Matt||Priscilla||Desyi||Sujith
|-
|[[File:Kaylas 3D idea.PNG |100px]][[File:Kaylas 3D reality.png |100px]]||[[File:TinkerCad.png|100px]]||8||9||[[File:SujFlowCell.PNG|200px]]
|}

===Camera/Video Results===
{|align="center"
|1||A High Laminar Flow Day||Non-Laminar Flow Found||4||5
|-
|1||[[File:Done.png |200px]]||[[File:Morgan%27sFlow.PNG |200px]]||4||5
|}

{|align="center"
|6||7||8||9||Non-Laminar Flow
|-
|6||7||8||9||[[File:FlowSuj.gif]]
|}

Flow Cell Lab Activity

2016-04-08T05:22:38Z

Sujith: /* Camera/Video Results */

==Introduction to Flow Cells==
[[File:Yflow_cell.PNG|thumb|100px|frame|right|Typical Y-Flow Cell with Laminar Flow]]

The purpose of this experiment was to see if distinguishing a flow cells mixing area would change the flow through the system. In a regular T- or Y-flow cell there is a laminar flow, a flow in which the two sides do not mix, that can be manipulated to give a non-laminar flow through the system. Using ten different manipulation of a typical flow cell, a way of having a non-laminar flow was experimented. Blue and yellow dyed RO water was used to determine the laminar/non-laminar flow of the flow cells by way of peristaltic flow pumps and gravity filtration. These flow cells were created using TinkerCAD to make a virtual object, 3D printed, and cast with silicon. To attach the pumps, either peristaltic or gravity, to the flow cell, an acrylic plastic was drilled and tapped at the precise measurements of the individual flow cells created. Each flow cell was used and it was determined which resulted in laminar flow and which resulted in non-laminar flow as shown below.

==Producing The Virtual Object==

===Getting Started with TinkerCAD===
* Go to the TinkerCAD website (https://www.tinkercad.com/)
* Create an account or Login to an existing account
* Click on Create a New Design to get started.
* There are tutorials available in TinkerCAD to get used to object placements and using different shapes. Also refer this link (https://www.tinkercad.com/quests/)

===Dimensions of the Mold===
* The Outer Box : 56 mm X 26 mm with a height of 7 mm with a 2 mm bottom
* Interior Channels : Channel height is 2 mm and the width of each channel is 2 mm

===Things to Note===
* Use cylinders for mixing chambers instead of spheres to avoid undercuts.
* For channels, ideally use boxes instead of half cylinders or cylinders.

===Exporting the file===
Once the design is complete. Click the "Design" tab located to the top left corner of the screen and export the file as .STL

{|align="center"
|
||[[File:Tinker1.png|thumb|400px|right|Box Dimensions]]
||[[File:Tinker2.png|thumb|400px|right|Exporting File]]
|}

==Prep for 3d Printing==

A gcode file is required in order to print an object on the 3D printer. This file can be created by exporting one's Tinkercad object as an STL. The STL file can be imported into Matter Control.

The gcode file contains settings specific to the 3D printer being used in production of the flow cell. As a result, one must ensure that the proper printer, material, and settings are chosen before exportation of the final gcode.
{|align="center"
|Rowspan="2"|[[File:Printer.PNG|200px|thumb|center|Select The Printer in Matter Control]]
||[[File:Settings_Layers.PNG |200px|thumb|right|Matter Control Layer Settings]]
||[[File:Settings_Infill.PNG|200px|thumb|right|Matter Control Infill Settings]]
||[[File:Settings_Raft.PNG |200px|thumb|right|Matter Control Raft Settings]]
|-
||[[File:Settings_Support.PNG|200px|thumb|right|Matter Control Support Settings]]
||[[File:Settings_Filament_Filament.PNG|200px|thumb|right|Matter Control Filament Settings]]
||[[File:Settings_Filament_Cooling.PNG|200px|thumb|right|Matter Control Cooling Settings]]
|}

==3d Printing==

==Silicon Casting==

After the 3D-printed cast has been made, now the actual flow cell can be created. We have used a 10:1 ratio of Slygard 184 (a silicon monomer) to curating agent, or about 5 grams Slygard 184 to 0.5 grams curating agent. After this is mixed thoroughly, we pour it into the cast and place it in a desiccator and place a vacuum on it to let the air bubbles come to the surface; once the vacuum is taken off, the bubbles will pop if left long enough.

[[File:Cast in desiccator.jpg|200px|thumb|center|Matter Control Layer Settings]]

After this, we can leave the cast to curate, or let the silicon harden and shape to the cast. There were a few ways this was done. Some put their cast into the oven, but due to the low melting temperature of the acrylic plastic, they started to melt, making them unable to be reused. Others, however, left theirs out over the time span of a week to harden. A table of how they have been done is listed below.

[[File:Screen Shot 2016-04-07 at 4.01.54 PM.png|600px|thumb|center|Matter Control Layer Settings]]

==Flow Cell Construction==
[[File:Brads Flow Cell.PNG|200px]]

==Flow Cell Experimentation==
===Flow Cell Designs===

{|align="center"
| Brad ||Chris||Morgan||Tyler||Ian
|-
|1||[[File:Chris_Tinker.PNG |200px]][[File:Chris_Printed.png|200px]]||[[File:Morgan%27sVirtual.PNG|100px]][[File:MReality.PNG |100px]]||[[File:Tylers Tinkercad.PNG|100px]][[File:Tylers mould.PNG |100px]]||[[File:Ian's_Mixer_Thing.PNG|200px]]
|}

{|align="center"
| Kayla||Matt||Priscilla||Desyi||Sujith
|-
|[[File:Kaylas 3D idea.PNG |100px]][[File:Kaylas 3D reality.png |100px]]||[[File:TinkerCad.png|100px]]||8||9||[[File:SujFlowCell.PNG|200px]]
|}

===Camera/Video Results===
{|align="left"
|1||[[File:Done.png |200px]]||[[File:Morgan%27sFlow.PNG |200px]]||4||5
|-
|1||A High Laminar Flow Day||Non-Laminar Flow Found||4||5
|}

{|align="left"
|6||7||8||9||[[File:FlowSuj.gif|200px]]
|-
|6||7||8||9||Non-Laminar Flow
|}

File:FlowSuj.gif

2016-04-08T05:21:53Z

Sujith: Sujith uploaded a new version of File:FlowSuj.gif

File:FlowSuj.gif

2016-04-08T05:19:46Z

Sujith:

Flow Cell Lab Activity

2016-04-08T05:07:44Z

Sujith: /* Flow Cell Experimentation */

==Introduction to Flow Cells==
[[File:Yflow_cell.PNG|thumb|100px|frame|right|Typical Y-Flow Cell with Laminar Flow]]

The purpose of this experiment was to see if distinguishing a flow cells mixing area would change the flow through the system. In a regular T- or Y-flow cell there is a laminar flow, a flow in which the two sides do not mix, that can be manipulated to give a non-laminar flow through the system. Using ten different manipulation of a typical flow cell, a way of having a non-laminar flow was experimented. Blue and yellow dyed RO water was used to determine the laminar/non-laminar flow of the flow cells by way of peristaltic flow pumps and gravity filtration. These flow cells were created using TinkerCAD to make a virtual object, 3D printed, and cast with silicon. To attach the pumps, either peristaltic or gravity, to the flow cell, an acrylic plastic was drilled and tapped at the precise measurements of the individual flow cells created. Each flow cell was used and it was determined which resulted in laminar flow and which resulted in non-laminar flow as shown below.

==Producing The Virtual Object==

===Getting Started with TinkerCAD===
* Go to the TinkerCAD website (https://www.tinkercad.com/)
* Create an account or Login to an existing account
* Click on Create a New Design to get started.
* There are tutorials available in TinkerCAD to get used to object placements and using different shapes. Also refer this link (https://www.tinkercad.com/quests/)

===Dimensions of the Mold===
* The Outer Box : 56 mm X 26 mm with a height of 7 mm with a 2 mm bottom
* Interior Channels : Channel height is 2 mm and the width of each channel is 2 mm

===Things to Note===
* Use cylinders for mixing chambers instead of spheres to avoid undercuts.
* For channels, ideally use boxes instead of half cylinders or cylinders.

===Exporting the file===
Once the design is complete. Click the "Design" tab located to the top left corner of the screen and export the file as .STL

{|align="center"
|
||[[File:Tinker1.png|thumb|400px|right|Box Dimensions]]
||[[File:Tinker2.png|thumb|400px|right|Exporting File]]
|}

==Prep for 3d Printing==

A gcode file is required in order to print an object on the 3D printer. This file can be created by exporting one's Tinkercad object as an STL. The STL file can be imported into Matter Control.

The gcode file contains settings specific to the 3D printer being used in production of the flow cell. As a result, one must ensure that the proper printer, material, and settings are chosen before exportation of the final gcode.
{|align="center"
|Rowspan="2"|[[File:Printer.PNG|200px|thumb|center|Select The Printer in Matter Control]]
||[[File:Settings_Layers.PNG |200px|thumb|right|Matter Control Layer Settings]]
||[[File:Settings_Infill.PNG|200px|thumb|right|Matter Control Infill Settings]]
||[[File:Settings_Raft.PNG |200px|thumb|right|Matter Control Raft Settings]]
|-
||[[File:Settings_Support.PNG|200px|thumb|right|Matter Control Support Settings]]
||[[File:Settings_Filament_Filament.PNG|200px|thumb|right|Matter Control Filament Settings]]
||[[File:Settings_Filament_Cooling.PNG|200px|thumb|right|Matter Control Cooling Settings]]
|}

==3d Printing==

==Silicon Casting==

After the 3D-printed cast has been made, now the actual flow cell can be created. We have used a 10:1 ratio of Slygard 184 (a silicon monomer) to curating agent, or about 5 grams Slygard 184 to 0.5 grams curating agent. After this is mixed thoroughly, we pour it into the cast and place it in a desiccator and place a vacuum on it to let the air bubbles come to the surface; once the vacuum is taken off, the bubbles will pop if left long enough.

[[File:Cast in desiccator.jpg|200px|thumb|center|Matter Control Layer Settings]]

After this, we can leave the cast to curate, or let the silicon harden and shape to the cast. There were a few ways this was done. Some put their cast into the oven, but due to the low melting temperature of the acrylic plastic, they started to melt, making them unable to be reused. Others, however, left theirs out over the time span of a week to harden. A table of how they have been done is listed below.

[[File:Screen Shot 2016-04-07 at 4.01.54 PM.png|600px|thumb|center|Matter Control Layer Settings]]

==Flow Cell Construction==
[[File:Brads Flow Cell.PNG|200px]]

==Flow Cell Experimentation==
===Flow Cell Designs===

{|align="center"
| Brad ||Chris||Morgan||Tyler||Ian
|-
|1||[[File:Chris_Tinker.PNG |200px]][[File:Chris_Printed.png|200px]]||[[File:Morgan%27sVirtual.PNG|100px]][[File:MReality.PNG |100px]]||[[File:Tylers Tinkercad.PNG|100px]][[File:Tylers mould.PNG |100px]]||[[File:Ian's_Mixer_Thing.PNG|200px]]
|}

{|align="center"
| Kayla||Matt||Priscilla||Desyi||Sujith
|-
|[[File:Kaylas 3D idea.PNG |100px]][[File:Kaylas 3D reality.png |100px]]||[[File:TinkerCad.png|100px]]||8||9||[[File:SujFlowCell.PNG|200px]]
|}

===Camera/Video Results===
{|align="left"
|1||[[File:Done.png |200px]]||[[File:Morgan%27sFlow.PNG |200px]]||4||5||6||7||8||9||10
|-
|1||A High Laminar Flow Day||Non-Laminar Flow Found||4||5||6||7||8||9||10
|}

Flow Cell Lab Activity

2016-04-08T05:07:19Z

Sujith: /* Flow Cell Designs */

==Introduction to Flow Cells==
[[File:Yflow_cell.PNG|thumb|100px|frame|right|Typical Y-Flow Cell with Laminar Flow]]

The purpose of this experiment was to see if distinguishing a flow cells mixing area would change the flow through the system. In a regular T- or Y-flow cell there is a laminar flow, a flow in which the two sides do not mix, that can be manipulated to give a non-laminar flow through the system. Using ten different manipulation of a typical flow cell, a way of having a non-laminar flow was experimented. Blue and yellow dyed RO water was used to determine the laminar/non-laminar flow of the flow cells by way of peristaltic flow pumps and gravity filtration. These flow cells were created using TinkerCAD to make a virtual object, 3D printed, and cast with silicon. To attach the pumps, either peristaltic or gravity, to the flow cell, an acrylic plastic was drilled and tapped at the precise measurements of the individual flow cells created. Each flow cell was used and it was determined which resulted in laminar flow and which resulted in non-laminar flow as shown below.

==Producing The Virtual Object==

===Getting Started with TinkerCAD===
* Go to the TinkerCAD website (https://www.tinkercad.com/)
* Create an account or Login to an existing account
* Click on Create a New Design to get started.
* There are tutorials available in TinkerCAD to get used to object placements and using different shapes. Also refer this link (https://www.tinkercad.com/quests/)

===Dimensions of the Mold===
* The Outer Box : 56 mm X 26 mm with a height of 7 mm with a 2 mm bottom
* Interior Channels : Channel height is 2 mm and the width of each channel is 2 mm

===Things to Note===
* Use cylinders for mixing chambers instead of spheres to avoid undercuts.
* For channels, ideally use boxes instead of half cylinders or cylinders.

===Exporting the file===
Once the design is complete. Click the "Design" tab located to the top left corner of the screen and export the file as .STL

{|align="center"
|
||[[File:Tinker1.png|thumb|400px|right|Box Dimensions]]
||[[File:Tinker2.png|thumb|400px|right|Exporting File]]
|}

==Prep for 3d Printing==

A gcode file is required in order to print an object on the 3D printer. This file can be created by exporting one's Tinkercad object as an STL. The STL file can be imported into Matter Control.

The gcode file contains settings specific to the 3D printer being used in production of the flow cell. As a result, one must ensure that the proper printer, material, and settings are chosen before exportation of the final gcode.
{|align="center"
|Rowspan="2"|[[File:Printer.PNG|200px|thumb|center|Select The Printer in Matter Control]]
||[[File:Settings_Layers.PNG |200px|thumb|right|Matter Control Layer Settings]]
||[[File:Settings_Infill.PNG|200px|thumb|right|Matter Control Infill Settings]]
||[[File:Settings_Raft.PNG |200px|thumb|right|Matter Control Raft Settings]]
|-
||[[File:Settings_Support.PNG|200px|thumb|right|Matter Control Support Settings]]
||[[File:Settings_Filament_Filament.PNG|200px|thumb|right|Matter Control Filament Settings]]
||[[File:Settings_Filament_Cooling.PNG|200px|thumb|right|Matter Control Cooling Settings]]
|}

==3d Printing==

==Silicon Casting==

After the 3D-printed cast has been made, now the actual flow cell can be created. We have used a 10:1 ratio of Slygard 184 (a silicon monomer) to curating agent, or about 5 grams Slygard 184 to 0.5 grams curating agent. After this is mixed thoroughly, we pour it into the cast and place it in a desiccator and place a vacuum on it to let the air bubbles come to the surface; once the vacuum is taken off, the bubbles will pop if left long enough.

[[File:Cast in desiccator.jpg|200px|thumb|center|Matter Control Layer Settings]]

After this, we can leave the cast to curate, or let the silicon harden and shape to the cast. There were a few ways this was done. Some put their cast into the oven, but due to the low melting temperature of the acrylic plastic, they started to melt, making them unable to be reused. Others, however, left theirs out over the time span of a week to harden. A table of how they have been done is listed below.

[[File:Screen Shot 2016-04-07 at 4.01.54 PM.png|600px|thumb|center|Matter Control Layer Settings]]

==Flow Cell Construction==
[[File:Brads Flow Cell.PNG|200px]]

==Flow Cell Experimentation==
===Flow Cell Designs===

{|align="left"
| Brad ||Chris||Morgan||Tyler||Ian
|-
|1||[[File:Chris_Tinker.PNG |200px]][[File:Chris_Printed.png|200px]]||[[File:Morgan%27sVirtual.PNG|100px]][[File:MReality.PNG |100px]]||[[File:Tylers Tinkercad.PNG|100px]][[File:Tylers mould.PNG |100px]]||[[File:Ian's_Mixer_Thing.PNG|200px]]
|}

{|align="left"
| Kayla||Matt||Priscilla||Desyi||Sujith
|-
|[[File:Kaylas 3D idea.PNG |100px]][[File:Kaylas 3D reality.png |100px]]||[[File:TinkerCad.png|100px]]||8||9||[[File:SujFlowCell.PNG|200px]]
|}

===Camera/Video Results===
{|align="left"
|1||[[File:Done.png |200px]]||[[File:Morgan%27sFlow.PNG |200px]]||4||5||6||7||8||9||10
|-
|1||A High Laminar Flow Day||Non-Laminar Flow Found||4||5||6||7||8||9||10
|}

Flow Cell Lab Activity

2016-04-08T05:02:10Z

Sujith: /* Flow Cell Experimentation */

==Introduction to Flow Cells==
[[File:Yflow_cell.PNG|thumb|100px|frame|right|Typical Y-Flow Cell with Laminar Flow]]

The purpose of this experiment was to see if distinguishing a flow cells mixing area would change the flow through the system. In a regular T- or Y-flow cell there is a laminar flow, a flow in which the two sides do not mix, that can be manipulated to give a non-laminar flow through the system. Using ten different manipulation of a typical flow cell, a way of having a non-laminar flow was experimented. Blue and yellow dyed RO water was used to determine the laminar/non-laminar flow of the flow cells by way of peristaltic flow pumps and gravity filtration. These flow cells were created using TinkerCAD to make a virtual object, 3D printed, and cast with silicon. To attach the pumps, either peristaltic or gravity, to the flow cell, an acrylic plastic was drilled and tapped at the precise measurements of the individual flow cells created. Each flow cell was used and it was determined which resulted in laminar flow and which resulted in non-laminar flow as shown below.

==Producing The Virtual Object==

===Getting Started with TinkerCAD===
* Go to the TinkerCAD website (https://www.tinkercad.com/)
* Create an account or Login to an existing account
* Click on Create a New Design to get started.
* There are tutorials available in TinkerCAD to get used to object placements and using different shapes. Also refer this link (https://www.tinkercad.com/quests/)

===Dimensions of the Mold===
* The Outer Box : 56 mm X 26 mm with a height of 7 mm with a 2 mm bottom
* Interior Channels : Channel height is 2 mm and the width of each channel is 2 mm

===Things to Note===
* Use cylinders for mixing chambers instead of spheres to avoid undercuts.
* For channels, ideally use boxes instead of half cylinders or cylinders.

===Exporting the file===
Once the design is complete. Click the "Design" tab located to the top left corner of the screen and export the file as .STL

{|align="center"
|
||[[File:Tinker1.png|thumb|400px|right|Box Dimensions]]
||[[File:Tinker2.png|thumb|400px|right|Exporting File]]
|}

==Prep for 3d Printing==

A gcode file is required in order to print an object on the 3D printer. This file can be created by exporting one's Tinkercad object as an STL. The STL file can be imported into Matter Control.

The gcode file contains settings specific to the 3D printer being used in production of the flow cell. As a result, one must ensure that the proper printer, material, and settings are chosen before exportation of the final gcode.
{|align="center"
|Rowspan="2"|[[File:Printer.PNG|200px|thumb|center|Select The Printer in Matter Control]]
||[[File:Settings_Layers.PNG |200px|thumb|right|Matter Control Layer Settings]]
||[[File:Settings_Infill.PNG|200px|thumb|right|Matter Control Infill Settings]]
||[[File:Settings_Raft.PNG |200px|thumb|right|Matter Control Raft Settings]]
|-
||[[File:Settings_Support.PNG|200px|thumb|right|Matter Control Support Settings]]
||[[File:Settings_Filament_Filament.PNG|200px|thumb|right|Matter Control Filament Settings]]
||[[File:Settings_Filament_Cooling.PNG|200px|thumb|right|Matter Control Cooling Settings]]
|}

==3d Printing==

==Silicon Casting==

After the 3D-printed cast has been made, now the actual flow cell can be created. We have used a 10:1 ratio of Slygard 184 (a silicon monomer) to curating agent, or about 5 grams Slygard 184 to 0.5 grams curating agent. After this is mixed thoroughly, we pour it into the cast and place it in a desiccator and place a vacuum on it to let the air bubbles come to the surface; once the vacuum is taken off, the bubbles will pop if left long enough.

[[File:Cast in desiccator.jpg|200px|thumb|center|Matter Control Layer Settings]]

After this, we can leave the cast to curate, or let the silicon harden and shape to the cast. There were a few ways this was done. Some put their cast into the oven, but due to the low melting temperature of the acrylic plastic, they started to melt, making them unable to be reused. Others, however, left theirs out over the time span of a week to harden. A table of how they have been done is listed below.

[[File:Screen Shot 2016-04-07 at 4.01.54 PM.png|600px|thumb|center|Matter Control Layer Settings]]

==Flow Cell Construction==
[[File:Brads Flow Cell.PNG|200px]]

==Flow Cell Experimentation==
===Flow Cell Designs===

{|align="center"
| Brad ||Chris||Morgan||Tyler||Ian||Kayla||Matt||Priscilla||Desyi||Sujith
|-
|1||[[File:Chris_Tinker.PNG |x200px]][[File:Chris_Printed.png|x200px]]||[[File:Morgan%27sVirtual.PNG|x200px]][[File:MReality.PNG |x200px]]||[[File:Tylers Tinkercad.PNG|x200px]][[File:Tylers mould.PNG |x200px]]||[[File:Ian's_Mixer_Thing.PNG|x200px]]||[[File:Kaylas 3D idea.PNG |x200px]][[File:Kaylas 3D reality.png |x200px]]||[[File:TinkerCad.png|x200px]]||8||9||[[File:SujFlowCell.PNG|x200px]]
|}

===Camera/Video Results===
{|align="left"
|1||[[File:Done.png |200px]]||[[File:Morgan%27sFlow.PNG |200px]]||4||5||6||7||8||9||10
|-
|1||A High Laminar Flow Day||Non-Laminar Flow Found||4||5||6||7||8||9||10
|}

File:SujFlowCell.PNG

2016-04-08T04:56:56Z

Sujith:

Flow Cell Lab Activity

2016-04-08T04:52:52Z

Sujith: /* Flow Cell Experimentation */

==Introduction to Flow Cells==
[[File:Yflow_cell.PNG|thumb|100px|frame|right|Typical Y-Flow Cell with Laminar Flow]]

The purpose of this experiment was to see if distinguishing a flow cells mixing area would change the flow through the system. In a regular T- or Y-flow cell there is a laminar flow, a flow in which the two sides do not mix, that can be manipulated to give a non-laminar flow through the system. Using ten different manipulation of a typical flow cell, a way of having a non-laminar flow was experimented. Blue and yellow dyed RO water was used to determine the laminar/non-laminar flow of the flow cells by way of peristaltic flow pumps and gravity filtration. These flow cells were created using TinkerCAD to make a virtual object, 3D printed, and cast with silicon. To attach the pumps, either peristaltic or gravity, to the flow cell, an acrylic plastic was drilled and tapped at the precise measurements of the individual flow cells created. Each flow cell was used and it was determined which resulted in laminar flow and which resulted in non-laminar flow as shown below.

==Producing The Virtual Object==

===Getting Started with TinkerCAD===
* Go to the TinkerCAD website (https://www.tinkercad.com/)
* Create an account or Login to an existing account
* Click on Create a New Design to get started.
* There are tutorials available in TinkerCAD to get used to object placements and using different shapes. Also refer this link (https://www.tinkercad.com/quests/)

===Dimensions of the Mold===
* The Outer Box : 56 mm X 26 mm with a height of 7 mm with a 2 mm bottom
* Interior Channels : Channel height is 2 mm and the width of each channel is 2 mm

===Things to Note===
* Use cylinders for mixing chambers instead of spheres to avoid undercuts.
* For channels, ideally use boxes instead of half cylinders or cylinders.

===Exporting the file===
Once the design is complete. Click the "Design" tab located to the top left corner of the screen and export the file as .STL

{|align="center"
|
||[[File:Tinker1.png|thumb|400px|right|Box Dimensions]]
||[[File:Tinker2.png|thumb|400px|right|Exporting File]]
|}

==Prep for 3d Printing==

A gcode file is required in order to print an object on the 3D printer. This file can be created by exporting one's Tinkercad object as an STL. The STL file can be imported into Matter Control.

The gcode file contains settings specific to the 3D printer being used in production of the flow cell. As a result, one must ensure that the proper printer, material, and settings are chosen before exportation of the final gcode.
{|align="center"
|Rowspan="2"|[[File:Printer.PNG|200px|thumb|center|Select The Printer in Matter Control]]
||[[File:Settings_Layers.PNG |200px|thumb|right|Matter Control Layer Settings]]
||[[File:Settings_Infill.PNG|200px|thumb|right|Matter Control Infill Settings]]
||[[File:Settings_Raft.PNG |200px|thumb|right|Matter Control Raft Settings]]
|-
||[[File:Settings_Support.PNG|200px|thumb|right|Matter Control Support Settings]]
||[[File:Settings_Filament_Filament.PNG|200px|thumb|right|Matter Control Filament Settings]]
||[[File:Settings_Filament_Cooling.PNG|200px|thumb|right|Matter Control Cooling Settings]]
|}

==3d Printing==

==Silicon Casting==

After the 3D-printed cast has been made, now the actual flow cell can be created. We have used a 10:1 ratio of Slygard 184 (a silicon monomer) to curating agent, or about 5 grams Slygard 184 to 0.5 grams curating agent. After this is mixed thoroughly, we pour it into the cast and place it in a desiccator and place a vacuum on it to let the air bubbles come to the surface; once the vacuum is taken off, the bubbles will pop if left long enough.

[[File:Cast in desiccator.jpg|200px|thumb|center|Matter Control Layer Settings]]

After this, we can leave the cast to curate, or let the silicon harden and shape to the cast. There were a few ways this was done. Some put their cast into the oven, but due to the low melting temperature of the acrylic plastic, they started to melt, making them unable to be reused. Others, however, left theirs out over the time span of a week to harden. A table of how they have been done is listed below.

[[File:Screen Shot 2016-04-07 at 4.01.54 PM.png|600px|thumb|center|Matter Control Layer Settings]]

==Flow Cell Construction==
[[File:Brads Flow Cell.PNG|200px]]

==Flow Cell Experimentation==
===Flow Cell Designs===

{|align="center"
| Brad ||Chris||Morgan||Tyler||Ian||Kayla||Matt||Priscilla||Desyi||Sujith
|-
|1||[[File:Chris_Tinker.PNG |200px]][[File:Chris_Printed.png|200px]]||[[File:Morgan%27sVirtual.PNG|100px]][[File:MReality.PNG |100px]]||[[File:Tylers Tinkercad.PNG|100px]][[File:Tylers mould.PNG |100px]]||[[File:Ian's_Mixer_Thing.PNG|200px]]||[[File:Kaylas 3D idea.PNG |150px]][[File:Kaylas 3D reality.png |150px]]||[[File:TinkerCad.png|100px]]||8||9||10
|}

===Camera/Video Results===
{|align="left"
|1||[[File:Done.png |200px]]||[[File:Morgan%27sFlow.PNG |200px]]||4||5||6||7||8||9||10
|-
|1||A High Laminar Flow Day||Non-Laminar Flow Found||4||5||6||7||8||9||10
|}

Flow Cell Lab Activity

2016-04-08T04:49:39Z

Sujith: /* Producing The Virtual Object */

==Introduction to Flow Cells==
[[File:Yflow_cell.PNG|thumb|100px|frame|right|Typical Y-Flow Cell with Laminar Flow]]

The purpose of this experiment was to see if distinguishing a flow cells mixing area would change the flow through the system. In a regular T- or Y-flow cell there is a laminar flow, a flow in which the two sides do not mix, that can be manipulated to give a non-laminar flow through the system. Using ten different manipulation of a typical flow cell, a way of having a non-laminar flow was experimented. Blue and yellow dyed RO water was used to determine the laminar/non-laminar flow of the flow cells by way of peristaltic flow pumps and gravity filtration. These flow cells were created using TinkerCAD to make a virtual object, 3D printed, and cast with silicon. To attach the pumps, either peristaltic or gravity, to the flow cell, an acrylic plastic was drilled and tapped at the precise measurements of the individual flow cells created. Each flow cell was used and it was determined which resulted in laminar flow and which resulted in non-laminar flow as shown below.

==Producing The Virtual Object==

===Getting Started with TinkerCAD===
* Go to the TinkerCAD website (https://www.tinkercad.com/)
* Create an account or Login to an existing account
* Click on Create a New Design to get started.
* There are tutorials available in TinkerCAD to get used to object placements and using different shapes. Also refer this link (https://www.tinkercad.com/quests/)

===Dimensions of the Mold===
* The Outer Box : 56 mm X 26 mm with a height of 7 mm with a 2 mm bottom
* Interior Channels : Channel height is 2 mm and the width of each channel is 2 mm

===Things to Note===
* Use cylinders for mixing chambers instead of spheres to avoid undercuts.
* For channels, ideally use boxes instead of half cylinders or cylinders.

===Exporting the file===
Once the design is complete. Click the "Design" tab located to the top left corner of the screen and export the file as .STL

{|align="center"
|
||[[File:Tinker1.png|thumb|400px|right|Box Dimensions]]
||[[File:Tinker2.png|thumb|400px|right|Exporting File]]
|}

==Prep for 3d Printing==

A gcode file is required in order to print an object on the 3D printer. This file can be created by exporting one's Tinkercad object as an STL. The STL file can be imported into Matter Control.

The gcode file contains settings specific to the 3D printer being used in production of the flow cell. As a result, one must ensure that the proper printer, material, and settings are chosen before exportation of the final gcode.
{|align="center"
|Rowspan="2"|[[File:Printer.PNG|200px|thumb|center|Select The Printer in Matter Control]]
||[[File:Settings_Layers.PNG |200px|thumb|right|Matter Control Layer Settings]]
||[[File:Settings_Infill.PNG|200px|thumb|right|Matter Control Infill Settings]]
||[[File:Settings_Raft.PNG |200px|thumb|right|Matter Control Raft Settings]]
|-
||[[File:Settings_Support.PNG|200px|thumb|right|Matter Control Support Settings]]
||[[File:Settings_Filament_Filament.PNG|200px|thumb|right|Matter Control Filament Settings]]
||[[File:Settings_Filament_Cooling.PNG|200px|thumb|right|Matter Control Cooling Settings]]
|}

==3d Printing==

==Silicon Casting==

After the 3D-printed cast has been made, now the actual flow cell can be created. We have used a 10:1 ratio of Slygard 184 (a silicon monomer) to curating agent, or about 5 grams Slygard 184 to 0.5 grams curating agent. After this is mixed thoroughly, we pour it into the cast and place it in a desiccator and place a vacuum on it to let the air bubbles come to the surface; once the vacuum is taken off, the bubbles will pop if left long enough.

[[File:Cast in desiccator.jpg|200px|thumb|center|Matter Control Layer Settings]]

After this, we can leave the cast to curate, or let the silicon harden and shape to the cast. There were a few ways this was done. Some put their cast into the oven, but due to the low melting temperature of the acrylic plastic, they started to melt, making them unable to be reused. Others, however, left theirs out over the time span of a week to harden. A table of how they have been done is listed below.

[[File:Screen Shot 2016-04-07 at 4.01.54 PM.png|600px|thumb|center|Matter Control Layer Settings]]

==Flow Cell Construction==
[[File:Brads Flow Cell.PNG|200px]]

==Flow Cell Experimentation==
video/camera
results

{|align="center"
| Brad ||Chris||Morgan||Tyler||Ian||Kayla||Matt||Priscilla||Desyi||Sujith
|-
|1||[[File:Chris_Tinker.PNG |200px]][[File:Chris_Printed.png|200px]]||[[File:Morgan%27sVirtual.PNG|100px]][[File:MReality.PNG |100px]]||[[File:Tylers Tinkercad.PNG|100px]][[File:Tylers mould.PNG |100px]]||[[File:Ian's_Mixer_Thing.PNG|200px]]||[[File:Kaylas 3D idea.PNG |150px]][[File:Kaylas 3D reality.png |150px]]||[[File:TinkerCad.png|100px]]||8||9||10
|-
|1||[[File:Done.png |200px]]||[[File:Morgan%27sFlow.PNG |200px]]||4||5||6||7||8||9||10
|-
|1||A High Laminar Flow Day||Non-Laminar Flow Found||4||5||6||7||8||9||10
|}

Flow Cell Lab Activity

2016-04-08T04:47:07Z

Sujith: /* Producing The Virtual Object */

==Introduction to Flow Cells==
[[File:Yflow_cell.PNG|thumb|100px|frame|right|Typical Y-Flow Cell with Laminar Flow]]

The purpose of this experiment was to see if distinguishing a flow cells mixing area would change the flow through the system. In a regular T- or Y-flow cell there is a laminar flow, a flow in which the two sides do not mix, that can be manipulated to give a non-laminar flow through the system. Using ten different manipulation of a typical flow cell, a way of having a non-laminar flow was experimented. Blue and yellow dyed RO water was used to determine the laminar/non-laminar flow of the flow cells by way of peristaltic flow pumps and gravity filtration. These flow cells were created using TinkerCAD to make a virtual object, 3D printed, and cast with silicon. To attach the pumps, either peristaltic or gravity, to the flow cell, an acrylic plastic was drilled and tapped at the precise measurements of the individual flow cells created. Each flow cell was used and it was determined which resulted in laminar flow and which resulted in non-laminar flow as shown below.

==Producing The Virtual Object==

===Getting Started with TinkerCAD===
* Go to the TinkerCAD website (https://www.tinkercad.com/)
* Create an account or Login to an existing account
* Click on Create a New Design to get started.
* There are tutorials available in TinkerCAD to get used to object placements and using different shapes. Also refer this link (https://www.tinkercad.com/quests/)

===Dimensions of the Mold===
* The Outer Box : 56 mm X 26 mm with a height of 7 mm with a 2 mm bottom
* Interior Channels : Channel height is 2 mm and the width of each channel is 2 mm
[[File:Tinker1.png|thumb|400px|right|Box Dimensions]]

===Things to Note===
* Use cylinders for mixing chambers instead of spheres to avoid undercuts.
* For channels, ideally use boxes instead of half cylinders or cylinders.

===Exporting the file===
Once the design is complete. Click the "Design" tab located to the top left corner of the screen and export the file as .STL
[[File:Tinker2.png|thumb|400px|right|Exporing File]]

==Prep for 3d Printing==

A gcode file is required in order to print an object on the 3D printer. This file can be created by exporting one's Tinkercad object as an STL. The STL file can be imported into Matter Control.

The gcode file contains settings specific to the 3D printer being used in production of the flow cell. As a result, one must ensure that the proper printer, material, and settings are chosen before exportation of the final gcode.
{|align="center"
|Rowspan="2"|[[File:Printer.PNG|200px|thumb|center|Select The Printer in Matter Control]]
||[[File:Settings_Layers.PNG |200px|thumb|right|Matter Control Layer Settings]]
||[[File:Settings_Infill.PNG|200px|thumb|right|Matter Control Infill Settings]]
||[[File:Settings_Raft.PNG |200px|thumb|right|Matter Control Raft Settings]]
|-
||[[File:Settings_Support.PNG|200px|thumb|right|Matter Control Support Settings]]
||[[File:Settings_Filament_Filament.PNG|200px|thumb|right|Matter Control Filament Settings]]
||[[File:Settings_Filament_Cooling.PNG|200px|thumb|right|Matter Control Cooling Settings]]
|}

==3d Printing==

==Silicon Casting==

After the 3D-printed cast has been made, now the actual flow cell can be created. We have used a 10:1 ratio of Slygard 184 (a silicon monomer) to curating agent, or about 5 grams Slygard 184 to 0.5 grams curating agent. After this is mixed thoroughly, we pour it into the cast and place it in a desiccator and place a vacuum on it to let the air bubbles come to the surface; once the vacuum is taken off, the bubbles will pop if left long enough.

[[File:Cast in desiccator.jpg|200px|thumb|center|Matter Control Layer Settings]]

After this, we can leave the cast to curate, or let the silicon harden and shape to the cast. There were a few ways this was done. Some put their cast into the oven, but due to the low melting temperature of the acrylic plastic, they started to melt, making them unable to be reused. Others, however, left theirs out over the time span of a week to harden. A table of how they have been done is listed below.

[[File:Screen Shot 2016-04-07 at 4.01.54 PM.png|600px|thumb|center|Matter Control Layer Settings]]

==Flow Cell Construction==
[[File:Brads Flow Cell.PNG|200px]]

==Flow Cell Experimentation==
video/camera
results

{|align="center"
| Brad ||Chris||Morgan||Tyler||Ian||Kayla||Matt||Priscilla||Desyi||Sujith
|-
|1||[[File:Chris_Tinker.PNG |200px]][[File:Chris_Printed.png|200px]]||[[File:Morgan%27sVirtual.PNG|100px]][[File:MReality.PNG |100px]]||[[File:Tylers Tinkercad.PNG|100px]][[File:Tylers mould.PNG |100px]]||[[File:Ian's_Mixer_Thing.PNG|200px]]||[[File:Kaylas 3D idea.PNG |150px]][[File:Kaylas 3D reality.png |150px]]||[[File:TinkerCad.png|100px]]||8||9||10
|-
|1||[[File:Done.png |200px]]||[[File:Morgan%27sFlow.PNG |200px]]||4||5||6||7||8||9||10
|-
|1||A High Laminar Flow Day||Non-Laminar Flow Found||4||5||6||7||8||9||10
|}

Flow Cell Lab Activity

2016-04-08T04:46:17Z

Sujith: /* Exporting the file */

==Introduction to Flow Cells==
[[File:Yflow_cell.PNG|thumb|100px|frame|right|Typical Y-Flow Cell with Laminar Flow]]

The purpose of this experiment was to see if distinguishing a flow cells mixing area would change the flow through the system. In a regular T- or Y-flow cell there is a laminar flow, a flow in which the two sides do not mix, that can be manipulated to give a non-laminar flow through the system. Using ten different manipulation of a typical flow cell, a way of having a non-laminar flow was experimented. Blue and yellow dyed RO water was used to determine the laminar/non-laminar flow of the flow cells by way of peristaltic flow pumps and gravity filtration. These flow cells were created using TinkerCAD to make a virtual object, 3D printed, and cast with silicon. To attach the pumps, either peristaltic or gravity, to the flow cell, an acrylic plastic was drilled and tapped at the precise measurements of the individual flow cells created. Each flow cell was used and it was determined which resulted in laminar flow and which resulted in non-laminar flow as shown below.

==Producing The Virtual Object==

===Getting Started with TinkerCAD===
* Go to the TinkerCAD website (https://www.tinkercad.com/)
* Create an account or Login to an existing account
* Click on Create a New Design to get started.
* There are tutorials available in TinkerCAD to get used to object placements and using different shapes. Also refer this link (https://www.tinkercad.com/quests/)

===Dimensions of the Mold===
* The Outer Box : 56 mm X 26 mm with a height of 7 mm with a 2 mm bottom
* Interior Channels : Channel height is 2 mm and the width of each channel is 2 mm
[[File:Tinker1.png|thumb|400px|left|Box Dimensions]]

===Things to Note===
* Use cylinders for mixing chambers instead of spheres to avoid undercuts.
* For channels, ideally use boxes instead of half cylinders or cylinders.

===Exporting the file===
Once the design is complete. Click the "Design" tab located to the top left corner of the screen and export the file as .STL
[[File:Tinker2.png|thumb|400px|left|Exporing File]]

==Prep for 3d Printing==

A gcode file is required in order to print an object on the 3D printer. This file can be created by exporting one's Tinkercad object as an STL. The STL file can be imported into Matter Control.

The gcode file contains settings specific to the 3D printer being used in production of the flow cell. As a result, one must ensure that the proper printer, material, and settings are chosen before exportation of the final gcode.
{|align="center"
|Rowspan="2"|[[File:Printer.PNG|200px|thumb|center|Select The Printer in Matter Control]]
||[[File:Settings_Layers.PNG |200px|thumb|right|Matter Control Layer Settings]]
||[[File:Settings_Infill.PNG|200px|thumb|right|Matter Control Infill Settings]]
||[[File:Settings_Raft.PNG |200px|thumb|right|Matter Control Raft Settings]]
|-
||[[File:Settings_Support.PNG|200px|thumb|right|Matter Control Support Settings]]
||[[File:Settings_Filament_Filament.PNG|200px|thumb|right|Matter Control Filament Settings]]
||[[File:Settings_Filament_Cooling.PNG|200px|thumb|right|Matter Control Cooling Settings]]
|}

==3d Printing==

==Silicon Casting==

After the 3D-printed cast has been made, now the actual flow cell can be created. We have used a 10:1 ratio of Slygard 184 (a silicon monomer) to curating agent, or about 5 grams Slygard 184 to 0.5 grams curating agent. After this is mixed thoroughly, we pour it into the cast and place it in a desiccator and place a vacuum on it to let the air bubbles come to the surface; once the vacuum is taken off, the bubbles will pop if left long enough.

[[File:Cast in desiccator.jpg|200px|thumb|center|Matter Control Layer Settings]]

After this, we can leave the cast to curate, or let the silicon harden and shape to the cast. There were a few ways this was done. Some put their cast into the oven, but due to the low melting temperature of the acrylic plastic, they started to melt, making them unable to be reused. Others, however, left theirs out over the time span of a week to harden. A table of how they have been done is listed below.

[[File:Screen Shot 2016-04-07 at 4.01.54 PM.png|600px|thumb|center|Matter Control Layer Settings]]

==Flow Cell Construction==
[[File:Brads Flow Cell.PNG|200px]]

==Flow Cell Experimentation==
video/camera
results

{|align="center"
| Brad ||Chris||Morgan||Tyler||Ian||Kayla||Matt||Priscilla||Desyi||Sujith
|-
|1||[[File:Chris_Tinker.PNG |200px]][[File:Chris_Printed.png|200px]]||[[File:Morgan%27sVirtual.PNG|100px]][[File:MReality.PNG |100px]]||[[File:Tylers Tinkercad.PNG|100px]][[File:Tylers mould.PNG |100px]]||[[File:Ian's_Mixer_Thing.PNG|200px]]||[[File:Kaylas 3D idea.PNG |150px]][[File:Kaylas 3D reality.png |150px]]||[[File:TinkerCad.png|100px]]||8||9||10
|-
|1||[[File:Done.png |200px]]||[[File:Morgan%27sFlow.PNG |200px]]||4||5||6||7||8||9||10
|-
|1||A High Laminar Flow Day||Non-Laminar Flow Found||4||5||6||7||8||9||10
|}

Flow Cell Lab Activity

2016-04-08T04:45:47Z

Sujith:

==Introduction to Flow Cells==
[[File:Yflow_cell.PNG|thumb|100px|frame|right|Typical Y-Flow Cell with Laminar Flow]]

The purpose of this experiment was to see if distinguishing a flow cells mixing area would change the flow through the system. In a regular T- or Y-flow cell there is a laminar flow, a flow in which the two sides do not mix, that can be manipulated to give a non-laminar flow through the system. Using ten different manipulation of a typical flow cell, a way of having a non-laminar flow was experimented. Blue and yellow dyed RO water was used to determine the laminar/non-laminar flow of the flow cells by way of peristaltic flow pumps and gravity filtration. These flow cells were created using TinkerCAD to make a virtual object, 3D printed, and cast with silicon. To attach the pumps, either peristaltic or gravity, to the flow cell, an acrylic plastic was drilled and tapped at the precise measurements of the individual flow cells created. Each flow cell was used and it was determined which resulted in laminar flow and which resulted in non-laminar flow as shown below.

==Producing The Virtual Object==

===Getting Started with TinkerCAD===
* Go to the TinkerCAD website (https://www.tinkercad.com/)
* Create an account or Login to an existing account
* Click on Create a New Design to get started.
* There are tutorials available in TinkerCAD to get used to object placements and using different shapes. Also refer this link (https://www.tinkercad.com/quests/)

===Dimensions of the Mold===
* The Outer Box : 56 mm X 26 mm with a height of 7 mm with a 2 mm bottom
* Interior Channels : Channel height is 2 mm and the width of each channel is 2 mm
[[File:Tinker1.png|thumb|400px|left|Box Dimensions]]

===Things to Note===
* Use cylinders for mixing chambers instead of spheres to avoid undercuts.
* For channels, ideally use boxes instead of half cylinders or cylinders.

===Exporting the file===
Once the design is complete. Click the "Design" tab located to the top left corner of the screen and export the file as .STL
[[File:Tinker2.png|thumb|400px|left|Box Dimensions]]

==Prep for 3d Printing==

A gcode file is required in order to print an object on the 3D printer. This file can be created by exporting one's Tinkercad object as an STL. The STL file can be imported into Matter Control.

The gcode file contains settings specific to the 3D printer being used in production of the flow cell. As a result, one must ensure that the proper printer, material, and settings are chosen before exportation of the final gcode.
{|align="center"
|Rowspan="2"|[[File:Printer.PNG|200px|thumb|center|Select The Printer in Matter Control]]
||[[File:Settings_Layers.PNG |200px|thumb|right|Matter Control Layer Settings]]
||[[File:Settings_Infill.PNG|200px|thumb|right|Matter Control Infill Settings]]
||[[File:Settings_Raft.PNG |200px|thumb|right|Matter Control Raft Settings]]
|-
||[[File:Settings_Support.PNG|200px|thumb|right|Matter Control Support Settings]]
||[[File:Settings_Filament_Filament.PNG|200px|thumb|right|Matter Control Filament Settings]]
||[[File:Settings_Filament_Cooling.PNG|200px|thumb|right|Matter Control Cooling Settings]]
|}

==3d Printing==

==Silicon Casting==

After the 3D-printed cast has been made, now the actual flow cell can be created. We have used a 10:1 ratio of Slygard 184 (a silicon monomer) to curating agent, or about 5 grams Slygard 184 to 0.5 grams curating agent. After this is mixed thoroughly, we pour it into the cast and place it in a desiccator and place a vacuum on it to let the air bubbles come to the surface; once the vacuum is taken off, the bubbles will pop if left long enough.

[[File:Cast in desiccator.jpg|200px|thumb|center|Matter Control Layer Settings]]

After this, we can leave the cast to curate, or let the silicon harden and shape to the cast. There were a few ways this was done. Some put their cast into the oven, but due to the low melting temperature of the acrylic plastic, they started to melt, making them unable to be reused. Others, however, left theirs out over the time span of a week to harden. A table of how they have been done is listed below.

[[File:Screen Shot 2016-04-07 at 4.01.54 PM.png|600px|thumb|center|Matter Control Layer Settings]]

==Flow Cell Construction==
[[File:Brads Flow Cell.PNG|200px]]

==Flow Cell Experimentation==
video/camera
results

{|align="center"
| Brad ||Chris||Morgan||Tyler||Ian||Kayla||Matt||Priscilla||Desyi||Sujith
|-
|1||[[File:Chris_Tinker.PNG |200px]][[File:Chris_Printed.png|200px]]||[[File:Morgan%27sVirtual.PNG|100px]][[File:MReality.PNG |100px]]||[[File:Tylers Tinkercad.PNG|100px]][[File:Tylers mould.PNG |100px]]||[[File:Ian's_Mixer_Thing.PNG|200px]]||[[File:Kaylas 3D idea.PNG |150px]][[File:Kaylas 3D reality.png |150px]]||[[File:TinkerCad.png|100px]]||8||9||10
|-
|1||[[File:Done.png |200px]]||[[File:Morgan%27sFlow.PNG |200px]]||4||5||6||7||8||9||10
|-
|1||A High Laminar Flow Day||Non-Laminar Flow Found||4||5||6||7||8||9||10
|}

Flow Cell Lab Activity

2016-04-08T04:43:29Z

Sujith: /* Producing The Virtual Object */

==Introduction to Flow Cells==
[[File:Yflow_cell.PNG|thumb|100px|frame|right|Typical Y-Flow Cell with Laminar Flow]]

The purpose of this experiment was to see if distinguishing a flow cells mixing area would change the flow through the system. In a regular T- or Y-flow cell there is a laminar flow, a flow in which the two sides do not mix, that can be manipulated to give a non-laminar flow through the system. Using ten different manipulation of a typical flow cell, a way of having a non-laminar flow was experimented. Blue and yellow dyed RO water was used to determine the laminar/non-laminar flow of the flow cells by way of peristaltic flow pumps and gravity filtration. These flow cells were created using TinkerCAD to make a virtual object, 3D printed, and cast with silicon. To attach the pumps, either peristaltic or gravity, to the flow cell, an acrylic plastic was drilled and tapped at the precise measurements of the individual flow cells created. Each flow cell was used and it was determined which resulted in laminar flow and which resulted in non-laminar flow as shown below.

==Producing The Virtual Object==

===Getting Started with TinkerCAD===
* Go to the TinkerCAD website (https://www.tinkercad.com/)
* Create an account or Login to an existing account
* Click on Create a New Design to get started.
* There are tutorials available in TinkerCAD to get used to object placements and using different shapes. Also refer this link (https://www.tinkercad.com/quests/)

===Dimensions of the Mold===
* The Outer Box : 56 mm X 26 mm with a height of 7 mm with a 2 mm bottom
* Interior Channels : Channel height is 2 mm and the width of each channel is 2 mm
[[File:Tinker1.png|thumb|400px|left|Box Dimensions]]

===Things to Note===
* Use cylinders for mixing chambers instead of spheres to avoid undercuts.
* For channels, ideally use boxes instead of half cylinders or cylinders.

===Exporting the file===
Once the design is complete. Click the "Design" tab located to the top left corner of the screen and export the file as .STL
[[File:Tinker2.png|thumb|400px|left|Box Dimensions]]

==Prep for 3d Printing==

A gcode file is required in order to print an object on the 3D printer. This file can be created by exporting one's Tinkercad object as an STL. The STL file can be imported into Matter Control.

The gcode file contains settings specific to the 3D printer being used in production of the flow cell. As a result, one must ensure that the proper printer, material, and settings are chosen before exportation of the final gcode.
{|align="center"
|Rowspan="2"|[[File:Printer.PNG|200px|thumb|center|Select The Printer in Matter Control]]
||[[File:Settings_Layers.PNG |200px|thumb|right|Matter Control Layer Settings]]
||[[File:Settings_Infill.PNG|200px|thumb|right|Matter Control Infill Settings]]
||[[File:Settings_Raft.PNG |200px|thumb|right|Matter Control Raft Settings]]
|-
||[[File:Settings_Support.PNG|200px|thumb|right|Matter Control Support Settings]]
||[[File:Settings_Filament_Filament.PNG|200px|thumb|right|Matter Control Filament Settings]]
||[[File:Settings_Filament_Cooling.PNG|200px|thumb|right|Matter Control Cooling Settings]]
|}

==3d Printing==

==Silicon Casting==

After the 3D-printed cast has been made, now the actual flow cell can be created. We have used a 10:1 ratio of Slygard 184 (a silicon monomer) to curating agent, or about 5 grams Slygard 184 to 0.5 grams curating agent. After this is mixed thoroughly, we pour it into the cast and place it in a desiccator and place a vacuum on it to let the air bubbles come to the surface; once the vacuum is taken off, the bubbles will pop if left long enough.

[[File:Cast in desiccator.jpg|200px|thumb|center|Matter Control Layer Settings]]

After this, we can leave the cast to curate, or let the silicon harden and shape to the cast. There were a few ways this was done. Some put their cast into the oven, but due to the low melting temperature of the acrylic plastic, they started to melt, making them unable to be reused. Others, however, left theirs out over the time span of a week to harden. A table of how they have been done is listed below.

[[File:Screen Shot 2016-04-07 at 4.01.54 PM.png|600px|thumb|center|Matter Control Layer Settings]]

==Flow Cell Construction==
[[File:Brads Flow Cell.PNG|200px]]

==Flow Cell Experimentation==
video/camera
results

{|align="center"
| Brad ||Chris||Morgan||Tyler||Ian||Kayla||Matt||Priscilla||Desyi||Sujith
|-
|1||[[File:Chris_Tinker.PNG |200px]][[File:Chris_Printed.png|200px]]||[[File:Morgan%27sVirtual.PNG|100px]][[File:MReality.PNG |100px]]||[[File:Tylers Tinkercad.PNG|100px]][[File:Tylers mould.PNG |100px]]||[[File:Ian's_Mixer_Thing.PNG|200px]]||[[File:Kaylas 3D idea.PNG |150px]][[File:Kaylas 3D reality.png |150px]]||[[File:TinkerCad.png|100px]]||8||9||10
|-
|1||[[File:Done.png |200px]]||[[File:Morgan%27sFlow.PNG |200px]]||4||5||6||7||8||9||10
|-
|1||A High Laminar Flow Day||Non-Laminar Flow Found||4||5||6||7||8||9||10
|}

Flow Cell Lab Activity

2016-04-08T04:41:54Z

Sujith: /* Exporting the file */

==Introduction to Flow Cells==
[[File:Yflow_cell.PNG|thumb|100px|frame|right|Typical Y-Flow Cell with Laminar Flow]]

The purpose of this experiment was to see if distinguishing a flow cells mixing area would change the flow through the system. In a regular T- or Y-flow cell there is a laminar flow, a flow in which the two sides do not mix, that can be manipulated to give a non-laminar flow through the system. Using ten different manipulation of a typical flow cell, a way of having a non-laminar flow was experimented. Blue and yellow dyed RO water was used to determine the laminar/non-laminar flow of the flow cells by way of peristaltic flow pumps and gravity filtration. These flow cells were created using TinkerCAD to make a virtual object, 3D printed, and cast with silicon. To attach the pumps, either peristaltic or gravity, to the flow cell, an acrylic plastic was drilled and tapped at the precise measurements of the individual flow cells created. Each flow cell was used and it was determined which resulted in laminar flow and which resulted in non-laminar flow as shown below.

==Producing The Virtual Object==

===Getting Started with TinkerCAD===
* Go to the TinkerCAD website (https://www.tinkercad.com/)
* Create an account or Login to an existing account
* Click on Create a New Design to get started.
* There are tutorials available in TinkerCAD to get used to object placements and using different shapes. Also refer this link (https://www.tinkercad.com/quests/)

===Dimensions of the Mold===
* The Outer Box : 56 mm X 26 mm with a height of 7 mm with a 2 mm bottom
* Interior Channels : Channel height is 2 mm and the width of each channel is 2 mm

{|align="left"
||[[File:Tinker1.png|thumb|400px|left|Box Dimensions]]
|}

===Things to Note===
* Use cylinders for mixing chambers instead of spheres to avoid undercuts.
* For channels, ideally use boxes instead of half cylinders or cylinders.

===Exporting the file===
Once the design is complete. Click the "Design" tab located to the top left corner of the screen and export the file as .STL
[[File:Tinker2.png|thumb|400px|left|Box Dimensions]]

==Prep for 3d Printing==

A gcode file is required in order to print an object on the 3D printer. This file can be created by exporting one's Tinkercad object as an STL. The STL file can be imported into Matter Control.

The gcode file contains settings specific to the 3D printer being used in production of the flow cell. As a result, one must ensure that the proper printer, material, and settings are chosen before exportation of the final gcode.
{|align="center"
|Rowspan="2"|[[File:Printer.PNG|200px|thumb|center|Select The Printer in Matter Control]]
||[[File:Settings_Layers.PNG |200px|thumb|right|Matter Control Layer Settings]]
||[[File:Settings_Infill.PNG|200px|thumb|right|Matter Control Infill Settings]]
||[[File:Settings_Raft.PNG |200px|thumb|right|Matter Control Raft Settings]]
|-
||[[File:Settings_Support.PNG|200px|thumb|right|Matter Control Support Settings]]
||[[File:Settings_Filament_Filament.PNG|200px|thumb|right|Matter Control Filament Settings]]
||[[File:Settings_Filament_Cooling.PNG|200px|thumb|right|Matter Control Cooling Settings]]
|}

==3d Printing==

==Silicon Casting==

After the 3D-printed cast has been made, now the actual flow cell can be created. We have used a 10:1 ratio of Slygard 184 (a silicon monomer) to curating agent, or about 5 grams Slygard 184 to 0.5 grams curating agent. After this is mixed thoroughly, we pour it into the cast and place it in a desiccator and place a vacuum on it to let the air bubbles come to the surface; once the vacuum is taken off, the bubbles will pop if left long enough.

[[File:Cast in desiccator.jpg|200px|thumb|center|Matter Control Layer Settings]]

After this, we can leave the cast to curate, or let the silicon harden and shape to the cast. There were a few ways this was done. Some put their cast into the oven, but due to the low melting temperature of the acrylic plastic, they started to melt, making them unable to be reused. Others, however, left theirs out over the time span of a week to harden. A table of how they have been done is listed below.

[[File:Screen Shot 2016-04-07 at 4.01.54 PM.png|600px|thumb|center|Matter Control Layer Settings]]

==Flow Cell Construction==
[[File:Brads Flow Cell.PNG|200px]]

==Flow Cell Experimentation==
video/camera
results

{|align="center"
| Brad ||Chris||Morgan||Tyler||Ian||Kayla||Matt||Priscilla||Desyi||Sujith
|-
|1||[[File:Chris_Tinker.PNG |200px]][[File:Chris_Printed.png|200px]]||[[File:Morgan%27sVirtual.PNG|100px]][[File:MReality.PNG |100px]]||[[File:Tylers Tinkercad.PNG|100px]][[File:Tylers mould.PNG |100px]]||[[File:Ian's_Mixer_Thing.PNG|200px]]||[[File:Kaylas 3D idea.PNG |150px]][[File:Kaylas 3D reality.png |150px]]||[[File:TinkerCad.png|100px]]||8||9||10
|-
|1||[[File:Done.png |200px]]||[[File:Morgan%27sFlow.PNG |200px]]||4||5||6||7||8||9||10
|-
|1||A High Laminar Flow Day||Non-Laminar Flow Found||4||5||6||7||8||9||10
|}

Flow Cell Lab Activity

2016-04-08T04:40:54Z

Sujith: /* Producing The Virtual Object */

==Introduction to Flow Cells==
[[File:Yflow_cell.PNG|thumb|100px|frame|right|Typical Y-Flow Cell with Laminar Flow]]

The purpose of this experiment was to see if distinguishing a flow cells mixing area would change the flow through the system. In a regular T- or Y-flow cell there is a laminar flow, a flow in which the two sides do not mix, that can be manipulated to give a non-laminar flow through the system. Using ten different manipulation of a typical flow cell, a way of having a non-laminar flow was experimented. Blue and yellow dyed RO water was used to determine the laminar/non-laminar flow of the flow cells by way of peristaltic flow pumps and gravity filtration. These flow cells were created using TinkerCAD to make a virtual object, 3D printed, and cast with silicon. To attach the pumps, either peristaltic or gravity, to the flow cell, an acrylic plastic was drilled and tapped at the precise measurements of the individual flow cells created. Each flow cell was used and it was determined which resulted in laminar flow and which resulted in non-laminar flow as shown below.

==Producing The Virtual Object==

===Getting Started with TinkerCAD===
* Go to the TinkerCAD website (https://www.tinkercad.com/)
* Create an account or Login to an existing account
* Click on Create a New Design to get started.
* There are tutorials available in TinkerCAD to get used to object placements and using different shapes. Also refer this link (https://www.tinkercad.com/quests/)

===Dimensions of the Mold===
* The Outer Box : 56 mm X 26 mm with a height of 7 mm with a 2 mm bottom
* Interior Channels : Channel height is 2 mm and the width of each channel is 2 mm

{|align="left"
||[[File:Tinker1.png|thumb|400px|left|Box Dimensions]]
|}

===Things to Note===
* Use cylinders for mixing chambers instead of spheres to avoid undercuts.
* For channels, ideally use boxes instead of half cylinders or cylinders.

===Exporting the file===
Once the design is complete. Click the "Design" tab located to the top left corner of the screen and export the file as .STL

{|align="left"
||[[File:Tinker2.png|thumb|400px|left|Box Dimensions]]
|}

==Prep for 3d Printing==

A gcode file is required in order to print an object on the 3D printer. This file can be created by exporting one's Tinkercad object as an STL. The STL file can be imported into Matter Control.

The gcode file contains settings specific to the 3D printer being used in production of the flow cell. As a result, one must ensure that the proper printer, material, and settings are chosen before exportation of the final gcode.
{|align="center"
|Rowspan="2"|[[File:Printer.PNG|200px|thumb|center|Select The Printer in Matter Control]]
||[[File:Settings_Layers.PNG |200px|thumb|right|Matter Control Layer Settings]]
||[[File:Settings_Infill.PNG|200px|thumb|right|Matter Control Infill Settings]]
||[[File:Settings_Raft.PNG |200px|thumb|right|Matter Control Raft Settings]]
|-
||[[File:Settings_Support.PNG|200px|thumb|right|Matter Control Support Settings]]
||[[File:Settings_Filament_Filament.PNG|200px|thumb|right|Matter Control Filament Settings]]
||[[File:Settings_Filament_Cooling.PNG|200px|thumb|right|Matter Control Cooling Settings]]
|}

==3d Printing==

==Silicon Casting==

After the 3D-printed cast has been made, now the actual flow cell can be created. We have used a 10:1 ratio of Slygard 184 (a silicon monomer) to curating agent, or about 5 grams Slygard 184 to 0.5 grams curating agent. After this is mixed thoroughly, we pour it into the cast and place it in a desiccator and place a vacuum on it to let the air bubbles come to the surface; once the vacuum is taken off, the bubbles will pop if left long enough.

[[File:Cast in desiccator.jpg|200px|thumb|center|Matter Control Layer Settings]]

After this, we can leave the cast to curate, or let the silicon harden and shape to the cast. There were a few ways this was done. Some put their cast into the oven, but due to the low melting temperature of the acrylic plastic, they started to melt, making them unable to be reused. Others, however, left theirs out over the time span of a week to harden. A table of how they have been done is listed below.

[[File:Screen Shot 2016-04-07 at 4.01.54 PM.png|600px|thumb|center|Matter Control Layer Settings]]

==Flow Cell Construction==
[[File:Brads Flow Cell.PNG|200px]]

==Flow Cell Experimentation==
video/camera
results

{|align="center"
| Brad ||Chris||Morgan||Tyler||Ian||Kayla||Matt||Priscilla||Desyi||Sujith
|-
|1||[[File:Chris_Tinker.PNG |200px]][[File:Chris_Printed.png|200px]]||[[File:Morgan%27sVirtual.PNG|100px]][[File:MReality.PNG |100px]]||[[File:Tylers Tinkercad.PNG|100px]][[File:Tylers mould.PNG |100px]]||[[File:Ian's_Mixer_Thing.PNG|200px]]||[[File:Kaylas 3D idea.PNG |150px]][[File:Kaylas 3D reality.png |150px]]||[[File:TinkerCad.png|100px]]||8||9||10
|-
|1||[[File:Done.png |200px]]||[[File:Morgan%27sFlow.PNG |200px]]||4||5||6||7||8||9||10
|-
|1||A High Laminar Flow Day||Non-Laminar Flow Found||4||5||6||7||8||9||10
|}

File:Tinker2.png

2016-04-08T04:40:04Z

Sujith:

File:Tinker1.png

2016-04-08T04:36:53Z

Sujith:

Flow Cell Lab Activity

2016-04-08T04:30:43Z

Sujith: /* Introduction to Flow Cells */

==Introduction to Flow Cells==
[[File:Yflow_cell.PNG|thumb|100px|frame|right|Typical Y-Flow Cell with Laminar Flow]]

The purpose of this experiment was to see if distinguishing a flow cells mixing area would change the flow through the system. In a regular T- or Y-flow cell there is a laminar flow, a flow in which the two sides do not mix, that can be manipulated to give a non-laminar flow through the system. Using ten different manipulation of a typical flow cell, a way of having a non-laminar flow was experimented. Blue and yellow dyed RO water was used to determine the laminar/non-laminar flow of the flow cells by way of peristaltic flow pumps and gravity filtration. These flow cells were created using TinkerCAD to make a virtual object, 3D printed, and cast with silicon. To attach the pumps, either peristaltic or gravity, to the flow cell, an acrylic plastic was drilled and tapped at the precise measurements of the individual flow cells created. Each flow cell was used and it was determined which resulted in laminar flow and which resulted in non-laminar flow as shown below.

==Producing The Virtual Object==

===Getting Started with TinkerCAD===
* Go to the TinkerCAD website (https://www.tinkercad.com/)
* Create an account or Login to an existing account
* Click on Create a New Design to get started.
* There are tutorials available in TinkerCAD to get used to object placements and using different shapes. Also refer this link (https://www.tinkercad.com/quests/)

===Dimensions of the Mold===
* The Outer Box : 56 mm X 26 mm with a height of 7 mm with a 2 mm bottom
* Interior Channels : Channel height is 2 mm and the width of each channel is 2 mm

===Things to Note===
* Use cylinders for mixing chambers instead of spheres to avoid undercuts.
* For channels, ideally use boxes instead of half cylinders or cylinders.

===Exporting the file===
Once the design is complete. Click the "Design" tab located to the top left corner of the screen and export the file as .STL

==Prep for 3d Printing==

A gcode file is required in order to print an object on the 3D printer. This file can be created by exporting one's Tinkercad object as an STL. The STL file can be imported into Matter Control.

The gcode file contains settings specific to the 3D printer being used in production of the flow cell. As a result, one must ensure that the proper printer, material, and settings are chosen before exportation of the final gcode.
{|align="center"
|Rowspan="2"|[[File:Printer.PNG|200px|thumb|center|Select The Printer in Matter Control]]
||[[File:Settings_Layers.PNG |200px|thumb|right|Matter Control Layer Settings]]
||[[File:Settings_Infill.PNG|200px|thumb|right|Matter Control Infill Settings]]
||[[File:Settings_Raft.PNG |200px|thumb|right|Matter Control Raft Settings]]
|-
||[[File:Settings_Support.PNG|200px|thumb|right|Matter Control Support Settings]]
||[[File:Settings_Filament_Filament.PNG|200px|thumb|right|Matter Control Filament Settings]]
||[[File:Settings_Filament_Cooling.PNG|200px|thumb|right|Matter Control Cooling Settings]]
|}

==3d Printing==

==Silicon Casting==

After the 3D-printed cast has been made, now the actual flow cell can be created. We have used a 10:1 ratio of Slygard 184 (a silicon monomer) to curating agent, or about 5 grams Slygard 184 to 0.5 grams curating agent. After this is mixed thoroughly, we pour it into the cast and place it in a desiccator and place a vacuum on it to let the air bubbles come to the surface; once the vacuum is taken off, the bubbles will pop if left long enough.

[[File:Cast in desiccator.jpg|200px|thumb|center|Matter Control Layer Settings]]

After this, we can leave the cast to curate, or let the silicon harden and shape to the cast. There were a few ways this was done. Some put their cast into the oven, but due to the low melting temperature of the acrylic plastic, they started to melt, making them unable to be reused. Others, however, left theirs out over the time span of a week to harden. A table of how they have been done is listed below.

[[File:Screen Shot 2016-04-07 at 4.01.54 PM.png|600px|thumb|center|Matter Control Layer Settings]]

==Flow Cell Construction==
[[File:Brads Flow Cell.PNG|200px]]

==Flow Cell Experimentation==
video/camera
results

{|align="center"
| Brad ||Chris||Morgan||Tyler||Ian||Kayla||Matt||Priscilla||Desyi||Sujith
|-
|1||[[File:Chris_Tinker.PNG |200px]][[File:Chris_Printed.png|200px]]||[[File:Morgan%27sVirtual.PNG|100px]][[File:MReality.PNG |100px]]||[[File:Tylers Tinkercad.PNG|100px]][[File:Tylers mould.PNG |100px]]||[[File:Ian's_Mixer_Thing.PNG|200px]]||[[File:Kaylas 3D idea.PNG |150px]][[File:Kaylas 3D reality.png |150px]]||[[File:TinkerCad.png|100px]]||8||9||10
|-
|1||[[File:Done.png |200px]]||[[File:Morgan%27sFlow.PNG |200px]]||4||5||6||7||8||9||10
|-
|1||A High Laminar Flow Day||Non-Laminar Flow Found||4||5||6||7||8||9||10
|}

Flow Cell Lab Activity

2016-04-08T04:25:57Z

Sujith: /* Introduction to Flow Cells */

==Introduction to Flow Cells==
[[File:Yflow_cell.PNG|150px|thumb|right|Typical Y-Flow Cell with Laminar Flow]]

The purpose of this experiment was to see if distinguishing a flow cells mixing area would change the flow through the system. In a regular T- or Y-flow cell there is a laminar flow, a flow in which the two sides do not mix, that can be manipulated to give a non-laminar flow through the system. Using ten different manipulation of a typical flow cell, a way of having a non-laminar flow was experimented. Blue and yellow dyed RO water was used to determine the laminar/non-laminar flow of the flow cells by way of peristaltic flow pumps and gravity filtration. These flow cells were created using TinkerCAD to make a virtual object, 3D printed, and cast with silicon. To attach the pumps, either peristaltic or gravity, to the flow cell, an acrylic plastic was drilled and tapped at the precise measurements of the individual flow cells created. Each flow cell was used and it was determined which resulted in laminar flow and which resulted in non-laminar flow as shown below.

==Producing The Virtual Object==

===Getting Started with TinkerCAD===
* Go to the TinkerCAD website (https://www.tinkercad.com/)
* Create an account or Login to an existing account
* Click on Create a New Design to get started.
* There are tutorials available in TinkerCAD to get used to object placements and using different shapes. Also refer this link (https://www.tinkercad.com/quests/)

===Dimensions of the Mold===
* The Outer Box : 56 mm X 26 mm with a height of 7 mm with a 2 mm bottom
* Interior Channels : Channel height is 2 mm and the width of each channel is 2 mm

===Things to Note===
* Use cylinders for mixing chambers instead of spheres to avoid undercuts.
* For channels, ideally use boxes instead of half cylinders or cylinders.

===Exporting the file===
Once the design is complete. Click the "Design" tab located to the top left corner of the screen and export the file as .STL

==Prep for 3d Printing==

A gcode file is required in order to print an object on the 3D printer. This file can be created by exporting one's Tinkercad object as an STL. The STL file can be imported into Matter Control.

The gcode file contains settings specific to the 3D printer being used in production of the flow cell. As a result, one must ensure that the proper printer, material, and settings are chosen before exportation of the final gcode.
{|align="center"
|Rowspan="2"|[[File:Printer.PNG|200px|thumb|center|Select The Printer in Matter Control]]
||[[File:Settings_Layers.PNG |200px|thumb|right|Matter Control Layer Settings]]
||[[File:Settings_Infill.PNG|200px|thumb|right|Matter Control Infill Settings]]
||[[File:Settings_Raft.PNG |200px|thumb|right|Matter Control Raft Settings]]
|-
||[[File:Settings_Support.PNG|200px|thumb|right|Matter Control Support Settings]]
||[[File:Settings_Filament_Filament.PNG|200px|thumb|right|Matter Control Filament Settings]]
||[[File:Settings_Filament_Cooling.PNG|200px|thumb|right|Matter Control Cooling Settings]]
|}

==3d Printing==

==Silicon Casting==

After the 3D-printed cast has been made, now the actual flow cell can be created. We have used a 10:1 ratio of Slygard 184 (a silicon monomer) to curating agent, or about 5 grams Slygard 184 to 0.5 grams curating agent. After this is mixed thoroughly, we pour it into the cast and place it in a desiccator and place a vacuum on it to let the air bubbles come to the surface; once the vacuum is taken off, the bubbles will pop if left long enough.

[[File:Cast in desiccator.jpg|200px|thumb|center|Matter Control Layer Settings]]

After this, we can leave the cast to curate, or let the silicon harden and shape to the cast. There were a few ways this was done. Some put their cast into the oven, but due to the low melting temperature of the acrylic plastic, they started to melt, making them unable to be reused. Others, however, left theirs out over the time span of a week to harden. A table of how they have been done is listed below.

[[File:Screen Shot 2016-04-07 at 4.01.54 PM.png|600px|thumb|center|Matter Control Layer Settings]]

==Flow Cell Construction==
[[File:Brads Flow Cell.PNG|200px]]

==Flow Cell Experimentation==
video/camera
results

{|align="center"
| Brad ||Chris||Morgan||Tyler||Ian||Kayla||Matt||Priscilla||Desyi||Sujith
|-
|1||[[File:Chris_Tinker.PNG |200px]][[File:Chris_Printed.png|200px]]||[[File:Morgan%27sVirtual.PNG|100px]][[File:MReality.PNG |100px]]||[[File:Tylers Tinkercad.PNG|100px]][[File:Tylers mould.PNG |100px]]||[[File:Ian's_Mixer_Thing.PNG|200px]]||[[File:Kaylas 3D idea.PNG |150px]][[File:Kaylas 3D reality.png |150px]]||[[File:TinkerCad.png|100px]]||8||9||10
|-
|1||[[File:Done.png |200px]]||[[File:Morgan%27sFlow.PNG |200px]]||4||5||6||7||8||9||10
|-
|1||A High Laminar Flow Day||Non-Laminar Flow Found||4||5||6||7||8||9||10
|}

Flow Cell Lab Activity

2016-04-08T04:24:11Z

Sujith: /* Producing The Virtual Object */

==Introduction to Flow Cells==
[[File:Yflow_cell.PNG|200px|thumb|right|Typical Y-Flow Cell with Laminar Flow]]

The purpose of this experiment was to see if distinguishing a flow cells mixing area would change the flow through the system. In a regular T- or Y-flow cell there is a laminar flow, a flow in which the two sides do not mix, that can be manipulated to give a non-laminar flow through the system. Using ten different manipulation of a typical flow cell, a way of having a non-laminar flow was experimented. Blue and yellow dyed RO water was used to determine the laminar/non-laminar flow of the flow cells by way of parastaltic flow pumps and gravity filtration. These flow cells were created using Tinkercad to make a virtual object, 3D printed, and casted with silicon. To attach the pumps, either parastaltic or gravity, to the flow cell, an acrylic plastic was drilled and tapped at the precise measurements of the individual flow cells created. Each flow cell was used and it was determined which resulted in laminar flow and which resulted in non-laminar flow as shown below.

==Producing The Virtual Object==

===Getting Started with TinkerCAD===
* Go to the TinkerCAD website (https://www.tinkercad.com/)
* Create an account or Login to an existing account
* Click on Create a New Design to get started.
* There are tutorials available in TinkerCAD to get used to object placements and using different shapes. Also refer this link (https://www.tinkercad.com/quests/)

===Dimensions of the Mold===
* The Outer Box : 56 mm X 26 mm with a height of 7 mm with a 2 mm bottom
* Interior Channels : Channel height is 2 mm and the width of each channel is 2 mm

===Things to Note===
* Use cylinders for mixing chambers instead of spheres to avoid undercuts.
* For channels, ideally use boxes instead of half cylinders or cylinders.

===Exporting the file===
Once the design is complete. Click the "Design" tab located to the top left corner of the screen and export the file as .STL

==Prep for 3d Printing==

A gcode file is required in order to print an object on the 3D printer. This file can be created by exporting one's Tinkercad object as an STL. The STL file can be imported into Matter Control.

The gcode file contains settings specific to the 3D printer being used in production of the flow cell. As a result, one must ensure that the proper printer, material, and settings are chosen before exportation of the final gcode.
{|align="center"
|Rowspan="2"|[[File:Printer.PNG|200px|thumb|center|Select The Printer in Matter Control]]
||[[File:Settings_Layers.PNG |200px|thumb|right|Matter Control Layer Settings]]
||[[File:Settings_Infill.PNG|200px|thumb|right|Matter Control Infill Settings]]
||[[File:Settings_Raft.PNG |200px|thumb|right|Matter Control Raft Settings]]
|-
||[[File:Settings_Support.PNG|200px|thumb|right|Matter Control Support Settings]]
||[[File:Settings_Filament_Filament.PNG|200px|thumb|right|Matter Control Filament Settings]]
||[[File:Settings_Filament_Cooling.PNG|200px|thumb|right|Matter Control Cooling Settings]]
|}

==3d Printing==

==Silicon Casting==

After the 3D-printed cast has been made, now the actual flow cell can be created. We have used a 10:1 ratio of Slygard 184 (a silicon monomer) to curating agent, or about 5 grams Slygard 184 to 0.5 grams curating agent. After this is mixed thoroughly, we pour it into the cast and place it in a desiccator and place a vacuum on it to let the air bubbles come to the surface; once the vacuum is taken off, the bubbles will pop if left long enough.

[[File:Cast in desiccator.jpg|200px|thumb|center|Matter Control Layer Settings]]

After this, we can leave the cast to curate, or let the silicon harden and shape to the cast. There were a few ways this was done. Some put their cast into the oven, but due to the low melting temperature of the acrylic plastic, they started to melt, making them unable to be reused. Others, however, left theirs out over the time span of a week to harden. A table of how they have been done is listed below.

[[File:Screen Shot 2016-04-07 at 4.01.54 PM.png|600px|thumb|center|Matter Control Layer Settings]]

==Flow Cell Construction==
[[File:Brads Flow Cell.PNG|200px]]

==Flow Cell Experimentation==
video/camera
results

{|align="center"
| Brad ||Chris||Morgan||Tyler||Ian||Kayla||Matt||Priscilla||Desyi||Sujith
|-
|1||[[File:Chris_Tinker.PNG |200px]][[File:Chris_Printed.png|200px]]||[[File:Morgan%27sVirtual.PNG|100px]][[File:MReality.PNG |100px]]||[[File:Tylers Tinkercad.PNG|100px]][[File:Tylers mould.PNG |100px]]||[[File:Ian's_Mixer_Thing.PNG|200px]]||[[File:Kaylas 3D idea.PNG |150px]][[File:Kaylas 3D reality.png |150px]]||[[File:TinkerCad.png|100px]]||8||9||10
|-
|1||[[File:Done.png |200px]]||[[File:Morgan%27sFlow.PNG |200px]]||4||5||6||7||8||9||10
|-
|1||A High Laminar Flow Day||Non-Laminar Flow Found||4||5||6||7||8||9||10
|}

Flow Cell Lab Activity

2016-04-08T04:23:04Z

Sujith: /* Producing The Virtual Object */

==Introduction to Flow Cells==
[[File:Yflow_cell.PNG|200px|thumb|right|Typical Y-Flow Cell with Laminar Flow]]

The purpose of this experiment was to see if distinguishing a flow cells mixing area would change the flow through the system. In a regular T- or Y-flow cell there is a laminar flow, a flow in which the two sides do not mix, that can be manipulated to give a non-laminar flow through the system. Using ten different manipulation of a typical flow cell, a way of having a non-laminar flow was experimented. Blue and yellow dyed RO water was used to determine the laminar/non-laminar flow of the flow cells by way of parastaltic flow pumps and gravity filtration. These flow cells were created using Tinkercad to make a virtual object, 3D printed, and casted with silicon. To attach the pumps, either parastaltic or gravity, to the flow cell, an acrylic plastic was drilled and tapped at the precise measurements of the individual flow cells created. Each flow cell was used and it was determined which resulted in laminar flow and which resulted in non-laminar flow as shown below.

==Producing The Virtual Object==

===Getting Started with TinkerCAD===
* Go to the TinkerCAD website (https://www.tinkercad.com/)
* Create an account or Login to an existing account
* Click on Create a New Design to get started.
* There are tutorials available in TinkerCAD to get used to object placements and using different shapes. Also refer this link (https://www.tinkercad.com/quests/)

===Dimensions of the Mold===
* The Outer Box : 56 mm X 26 mm with a height of 7 mm with a 2 mm bottom
* Interior Channels : Channel height is 2 mm and the width of each channel is 2 mm

===Things to Note===
* Use cylinders for mixing chambers instead of spheres to avoid undercuts.
* For channels, ideally use boxes instead of half cylinders or cylinders.

==Prep for 3d Printing==

A gcode file is required in order to print an object on the 3D printer. This file can be created by exporting one's Tinkercad object as an STL. The STL file can be imported into Matter Control.

The gcode file contains settings specific to the 3D printer being used in production of the flow cell. As a result, one must ensure that the proper printer, material, and settings are chosen before exportation of the final gcode.
{|align="center"
|Rowspan="2"|[[File:Printer.PNG|200px|thumb|center|Select The Printer in Matter Control]]
||[[File:Settings_Layers.PNG |200px|thumb|right|Matter Control Layer Settings]]
||[[File:Settings_Infill.PNG|200px|thumb|right|Matter Control Infill Settings]]
||[[File:Settings_Raft.PNG |200px|thumb|right|Matter Control Raft Settings]]
|-
||[[File:Settings_Support.PNG|200px|thumb|right|Matter Control Support Settings]]
||[[File:Settings_Filament_Filament.PNG|200px|thumb|right|Matter Control Filament Settings]]
||[[File:Settings_Filament_Cooling.PNG|200px|thumb|right|Matter Control Cooling Settings]]
|}

==3d Printing==

==Silicon Casting==

After the 3D-printed cast has been made, now the actual flow cell can be created. We have used a 10:1 ratio of Slygard 184 (a silicon monomer) to curating agent, or about 5 grams Slygard 184 to 0.5 grams curating agent. After this is mixed thoroughly, we pour it into the cast and place it in a desiccator and place a vacuum on it to let the air bubbles come to the surface; once the vacuum is taken off, the bubbles will pop if left long enough.

[[File:Cast in desiccator.jpg|200px|thumb|center|Matter Control Layer Settings]]

After this, we can leave the cast to curate, or let the silicon harden and shape to the cast. There were a few ways this was done. Some put their cast into the oven, but due to the low melting temperature of the acrylic plastic, they started to melt, making them unable to be reused. Others, however, left theirs out over the time span of a week to harden. A table of how they have been done is listed below.

[[File:Screen Shot 2016-04-07 at 4.01.54 PM.png|600px|thumb|center|Matter Control Layer Settings]]

==Flow Cell Construction==
[[File:Brads Flow Cell.PNG|200px]]

==Flow Cell Experimentation==
video/camera
results

{|align="center"
| Brad ||Chris||Morgan||Tyler||Ian||Kayla||Matt||Priscilla||Desyi||Sujith
|-
|1||[[File:Chris_Tinker.PNG |200px]][[File:Chris_Printed.png|200px]]||[[File:Morgan%27sVirtual.PNG|100px]][[File:MReality.PNG |100px]]||[[File:Tylers Tinkercad.PNG|100px]][[File:Tylers mould.PNG |100px]]||[[File:Ian's_Mixer_Thing.PNG|200px]]||[[File:Kaylas 3D idea.PNG |150px]][[File:Kaylas 3D reality.png |150px]]||[[File:TinkerCad.png|100px]]||8||9||10
|-
|1||[[File:Done.png |200px]]||[[File:Morgan%27sFlow.PNG |200px]]||4||5||6||7||8||9||10
|-
|1||A High Laminar Flow Day||Non-Laminar Flow Found||4||5||6||7||8||9||10
|}

Silicone Molding

2016-03-31T21:03:17Z

Sujith: /* PDMS Mold Preparation Procedures */

This is a page for silicone molding using SYLGARD 184

==PDMS Mold Preparation Procedures==
Preparation of Silicone Monomer
* Use 10 parts of Sylgard 184(silicone monomer) and 1 part of the curing agent (by mass)
* Mix well with the end of a pipette or a skewer stick
* Pour it in your mold and pull the air using a desiccator

After this, put the mold with the silicone mixture in an oven with temperatures set according to working temperatures for about 20 mins

Mold Preparation [http://esr.monmsci.net/wiki/images/f/f6/PDMS_Mold_Preparation_Kassegne_MEMSLab.pdf See Link]

==Things to Note==
*If using plastic petri dishes for the molds, make sure to check the working temperature for the material you are using.
*If using PLA 3-D Printed Molds, note that the working temperature is 60 to 65 degree Celsius.

Monmouth Mushroom Project

2016-03-21T14:27:06Z

Sujith: /* Research on Mushrooms for Health Benifits */

Welcome to the Monmouth Mushroom (Mycology) Project.

Funded by a grant ($300) form the Monmouth College, Global Food Security Studies (GFSS) Program. The [[Mushroom_Proposal|grant]] was written and submitted by Sujith Santhosh in the Spring 2016 with advising from Bradley E. Sturgeon (Chemistry Department).

==Mushroom Growing Procedure==

A mushroom is called a "fruiting body" and can be produced in two ways: 1) through the propagation of the "spores", or 2) through the propagation of tissue extract. The use of tissue will be discussed here. There are three distinct steps in this process:

''Phase 1: Agar preparation and sterile culture''

a) Prepare sterile potato dextrose agar (PDA) in test tube slants.

b) Transfer mushroom tissue into sterile PDA slant.

c) Observe mycelium growth from sterile culture after ~ 1 week.

''Phase 2: Sterile transfer to grain spawn''

a) Prepare sterile/pasteurized grain media in quart or pint canning jars.

b) Transfer small mycelium/agar material to the grain media.

c) Observe the expansive mycelium growth throughout the grain spawn after ~1 week.

''Phase 3: Transfer to final substrate and induction of fruiting''

a) Prepare sterile/pasteurized straw substrate.

b) Transfer mycelium rich grain spawn to the straw substrate.

c) Allow time for expansive mycelium growth.

d) Provide small aeration holes which will promote fruiting.

==References==

[http://www.mykoweb.com/articles/cultivation.html Getting Started with Mushroom Cultivation]

==Other info==

Rye grow bags...like Mary.

http://everythingmushrooms.com/sterilized-rye-mushroom-grow-bag-instructions/

http://everythingmushrooms.com/blog/cathys-lab-running-oysters-sawdust-vs-grain-spawn-and-other-insights/

==Research on Mushrooms for Health Benifits==
*Mushroom: The New Superfood [http://esr.monmsci.net/wiki/images/9/90/Mushrooms-_The_New_Superfood.pdf See Link]
*Shiitake Mushroom [http://esr.monmsci.net/wiki/images/2/28/Shiitake_Mushroom_Memorial_Sloan-Kettering_Cancer_Center.pdf See Link]

File:Shiitake Mushroom Memorial Sloan-Kettering Cancer Center.pdf

2016-03-21T14:26:29Z

Sujith:

File:Mushrooms- The New Superfood.pdf

2016-03-21T14:24:04Z

Sujith:

Monmouth Mushroom Project

2016-03-21T14:21:32Z

Sujith:

Silicone Molding

2016-03-21T13:13:27Z

Sujith:

This is a page for silicone molding using SYLGARD 184

==PDMS Mold Preparation Procedures==
Mold Preparation [http://esr.monmsci.net/wiki/images/f/f6/PDMS_Mold_Preparation_Kassegne_MEMSLab.pdf See Link]

==Things to Note==
*If using plastic petri dishes for the molds, make sure to check the working temperature for the material you are using.
*If using PLA 3-D Printed Molds, note that the working temperature is 60 to 65 degree Celsius.

Silicone Molding

2016-03-17T22:23:43Z

Sujith:

File:PDMS Mold Preparation Kassegne MEMSLab.pdf

2016-03-17T22:18:07Z

Sujith: Procedure for creating a PDMS mold using SYLGARD 184

Procedure for creating a PDMS mold using SYLGARD 184

Silicone Molding

2016-03-17T22:16:28Z

Sujith: Created page with "This is a page for silicone molding using SYLGARD 184 ==PDMS Mold Preparation Procedures=="

This is a page for silicone molding using SYLGARD 184

==PDMS Mold Preparation Procedures==