http://205.166.159.208/wiki/api.php?action=feedcontributions&feedformat=atom&user=Sarahalang535 2026-05-03T17:12:41Z User contributions MediaWiki 1.35.5 http://205.166.159.208/wiki/index.php?title=Sarah_Lang_BIOC430_S17&diff=5293 2017-03-01T21:59:01Z

<p>Sarahalang535: /* Research Times */</p> <hr /> <div>Chemistry/Biochemistry Research 430<br /> :Spring 2017<br /> :Sarah Lang<br /> :Senior Biochemistry Major<br /> <br /> ==Research Times==<br /> '''Wed: 2 pm-4:30 pm, Thurs: 3:30 pm-5:00 pm'''<br /> :section 01 = 0.25 credit = 4 hours per week.<br /> <br /> Science Seminar: Feb 17th<br /> <br /> ==Proposed Research Project==<br /> ===The Enzymatic Oxidation of Eugenol and Methyleugenol===<br /> <br /> ===General Information===<br /> :Advisor: Brad Sturgeon<br /> :Other research student collaborators: <br /> :Other Research Collaborators:<br /> <br /> ===Proposal===<br /> The purpose of this project is to understand the enzymatic oxidation of biophenols--specifically, of eugenol and methyleugenol. Both eugenol and methyleugenol are commonly found in flavoring components of foods, and recently, connections have been made between methyleugenol (and to a lesser extent, eugenol) and increased incidence of malignant tumors at various tissue sites (''1''). Furthermore, studies have shown that both eugenol and methyleugenol undergo peroxidative metabolism to form free radicals, but little is known about the individual products of this process. The goal of this research project is to identify, collect, and separate the products formed during the oxidation of eugenol and methyleugenol, in order to eventually gain a better understanding of their biological activity.<br /> <br /> ===Instruments to be used===<br /> <br /> HPLC, <br /> Flash Chromotography, <br /> NMR<br /> <br /> ===References (2 minimum)===<br /> 1. Sipe, H. J., Lardinois, O. M., &amp; Mason, R. P. (2014). Free Radical Metabolism of Methyleugenol and Related Compounds. Chemical Research in Toxicology, 27(4), 483–489. http://doi.org/10.1021/tx400256b<br /> <br /> 2. Mastelić, J., Jerković, I., Blažević, I., Poljak-Blaži, M., Borović, S., Ivančić-Baće, I., … Müller, N. (2008). Comparative Study on the Antioxidant and Biological Activities of Carvacrol, Thymol, and Eugenol Derivatives. Journal of Agricultural and Food Chemistry, 56(11), 3989–3996. http://doi.org/10.1021/jf073272v<br /> <br /> 3. Thompson, David, et al. &quot;Peroxidase-catalyzed oxidation of eugenol: formation of a cytotoxic metabolite (s).&quot; Journal of Biological Chemistry 264.2 (1989): 1016-1021. https://www.ncbi.nlm.nih.gov/pubmed/2536013<br /> <br /> 4. Bodell, William J., et al. &quot;Oxidation of eugenol to form DNA adducts and 8-hydroxy-2'-deoxyguanosine: role of quinone methide derivative in DNA adduct formation.&quot; Carcinogenesis 19.3 (1998): 437-443. https://www.ncbi.nlm.nih.gov/pubmed/9525278<br /> <br /> ===Research pledge===<br /> I, Sarah Lang, have read the Chem/Bioc 430 course syllabus and understand the general structure and expectations of the research program. The above material was prepared after consultation, and in conjunction with my research advisor.</div>

Sarahalang535 http://205.166.159.208/wiki/index.php?title=Sarah_Lang_BIOC430_S17&diff=5292 2017-03-01T21:56:41Z

<p>Sarahalang535: /* References (2 minimum) */</p> <hr /> <div>Chemistry/Biochemistry Research 430<br /> :Spring 2017<br /> :Sarah Lang<br /> :Senior Biochemistry Major<br /> <br /> ==Research Times==<br /> '''Tues: 9 am-10:30 am, Wed: 2 pm-4:30 pm'''<br /> :section 01 = 0.25 credit = 4 hours per week.<br /> <br /> Science Seminar: Feb 17th<br /> <br /> ==Proposed Research Project==<br /> ===The Enzymatic Oxidation of Eugenol and Methyleugenol===<br /> <br /> ===General Information===<br /> :Advisor: Brad Sturgeon<br /> :Other research student collaborators: <br /> :Other Research Collaborators:<br /> <br /> ===Proposal===<br /> The purpose of this project is to understand the enzymatic oxidation of biophenols--specifically, of eugenol and methyleugenol. Both eugenol and methyleugenol are commonly found in flavoring components of foods, and recently, connections have been made between methyleugenol (and to a lesser extent, eugenol) and increased incidence of malignant tumors at various tissue sites (''1''). Furthermore, studies have shown that both eugenol and methyleugenol undergo peroxidative metabolism to form free radicals, but little is known about the individual products of this process. The goal of this research project is to identify, collect, and separate the products formed during the oxidation of eugenol and methyleugenol, in order to eventually gain a better understanding of their biological activity.<br /> <br /> ===Instruments to be used===<br /> <br /> HPLC, <br /> Flash Chromotography, <br /> NMR<br /> <br /> ===References (2 minimum)===<br /> 1. Sipe, H. J., Lardinois, O. M., &amp; Mason, R. P. (2014). Free Radical Metabolism of Methyleugenol and Related Compounds. Chemical Research in Toxicology, 27(4), 483–489. http://doi.org/10.1021/tx400256b<br /> <br /> 2. Mastelić, J., Jerković, I., Blažević, I., Poljak-Blaži, M., Borović, S., Ivančić-Baće, I., … Müller, N. (2008). Comparative Study on the Antioxidant and Biological Activities of Carvacrol, Thymol, and Eugenol Derivatives. Journal of Agricultural and Food Chemistry, 56(11), 3989–3996. http://doi.org/10.1021/jf073272v<br /> <br /> 3. Thompson, David, et al. &quot;Peroxidase-catalyzed oxidation of eugenol: formation of a cytotoxic metabolite (s).&quot; Journal of Biological Chemistry 264.2 (1989): 1016-1021. https://www.ncbi.nlm.nih.gov/pubmed/2536013<br /> <br /> 4. Bodell, William J., et al. &quot;Oxidation of eugenol to form DNA adducts and 8-hydroxy-2'-deoxyguanosine: role of quinone methide derivative in DNA adduct formation.&quot; Carcinogenesis 19.3 (1998): 437-443. https://www.ncbi.nlm.nih.gov/pubmed/9525278<br /> <br /> ===Research pledge===<br /> I, Sarah Lang, have read the Chem/Bioc 430 course syllabus and understand the general structure and expectations of the research program. The above material was prepared after consultation, and in conjunction with my research advisor.</div>

Sarahalang535 http://205.166.159.208/wiki/index.php?title=Sarah_Lang_BIOC430_S17&diff=5004 2017-01-30T03:35:50Z

<p>Sarahalang535: /* Research Times */</p> <hr /> <div>Chemistry/Biochemistry Research 430<br /> :Spring 2017<br /> :Sarah Lang<br /> :Senior Biochemistry Major<br /> <br /> ==Research Times==<br /> '''Tues: 9 am-10:30 am, Wed: 2 pm-4:30 pm'''<br /> :section 01 = 0.25 credit = 4 hours per week.<br /> <br /> ==Proposed Research Project==<br /> ===The Enzymatic Oxidation of Eugenol and Methyleugenol===<br /> <br /> ===General Information===<br /> :Advisor: Brad Sturgeon<br /> :Other research student collaborators: <br /> :Other Research Collaborators:<br /> <br /> ===Proposal===<br /> The purpose of this project is to understand the enzymatic oxidation of biophenols--specifically, of eugenol and methyleugenol. Both eugenol and methyleugenol are commonly found in flavoring components of foods, and recently, connections have been made between methyleugenol (and to a lesser extent, eugenol) and increased incidence of malignant tumors at various tissue sites (''1''). Furthermore, studies have shown that both eugenol and methyleugenol undergo peroxidative metabolism to form free radicals, but little is known about the individual products of this process. The goal of this research project is to identify, collect, and separate the products formed during the oxidation of eugenol and methyleugenol, in order to eventually gain a better understanding of their biological activity.<br /> <br /> ===Instruments to be used===<br /> <br /> HPLC, <br /> Flash Chromotography, <br /> NMR<br /> <br /> ===References (2 minimum)===<br /> 1. Sipe, H. J., Lardinois, O. M., &amp; Mason, R. P. (2014). Free Radical Metabolism of Methyleugenol and Related Compounds. Chemical Research in Toxicology, 27(4), 483–489. http://doi.org/10.1021/tx400256b<br /> <br /> 2. Mastelić, J., Jerković, I., Blažević, I., Poljak-Blaži, M., Borović, S., Ivančić-Baće, I., … Müller, N. (2008). Comparative Study on the Antioxidant and Biological Activities of Carvacrol, Thymol, and Eugenol Derivatives. Journal of Agricultural and Food Chemistry, 56(11), 3989–3996. http://doi.org/10.1021/jf073272v<br /> <br /> ===Research pledge===<br /> I, Sarah Lang, have read the Chem/Bioc 430 course syllabus and understand the general structure and expectations of the research program. The above material was prepared after consultation, and in conjunction with my research advisor.</div>

Sarahalang535 http://205.166.159.208/wiki/index.php?title=Sarah_Lang_BIOC430_S17&diff=5003 2017-01-30T03:35:30Z

<p>Sarahalang535: Created page with &quot;Chemistry/Biochemistry Research 430 :Spring 2017 :Sarah Lang :Senior Biochemistry Major ==Research Times== '''Tues: 9 am-10:30 am Wed: 2 pm-4:30 pm''' :section 01 = 0.25 cred...&quot;</p> <hr /> <div>Chemistry/Biochemistry Research 430<br /> :Spring 2017<br /> :Sarah Lang<br /> :Senior Biochemistry Major<br /> <br /> ==Research Times==<br /> '''Tues: 9 am-10:30 am<br /> Wed: 2 pm-4:30 pm'''<br /> :section 01 = 0.25 credit = 4 hours per week.<br /> <br /> ==Proposed Research Project==<br /> ===The Enzymatic Oxidation of Eugenol and Methyleugenol===<br /> <br /> ===General Information===<br /> :Advisor: Brad Sturgeon<br /> :Other research student collaborators: <br /> :Other Research Collaborators:<br /> <br /> ===Proposal===<br /> The purpose of this project is to understand the enzymatic oxidation of biophenols--specifically, of eugenol and methyleugenol. Both eugenol and methyleugenol are commonly found in flavoring components of foods, and recently, connections have been made between methyleugenol (and to a lesser extent, eugenol) and increased incidence of malignant tumors at various tissue sites (''1''). Furthermore, studies have shown that both eugenol and methyleugenol undergo peroxidative metabolism to form free radicals, but little is known about the individual products of this process. The goal of this research project is to identify, collect, and separate the products formed during the oxidation of eugenol and methyleugenol, in order to eventually gain a better understanding of their biological activity.<br /> <br /> ===Instruments to be used===<br /> <br /> HPLC, <br /> Flash Chromotography, <br /> NMR<br /> <br /> ===References (2 minimum)===<br /> 1. Sipe, H. J., Lardinois, O. M., &amp; Mason, R. P. (2014). Free Radical Metabolism of Methyleugenol and Related Compounds. Chemical Research in Toxicology, 27(4), 483–489. http://doi.org/10.1021/tx400256b<br /> <br /> 2. Mastelić, J., Jerković, I., Blažević, I., Poljak-Blaži, M., Borović, S., Ivančić-Baće, I., … Müller, N. (2008). Comparative Study on the Antioxidant and Biological Activities of Carvacrol, Thymol, and Eugenol Derivatives. Journal of Agricultural and Food Chemistry, 56(11), 3989–3996. http://doi.org/10.1021/jf073272v<br /> <br /> ===Research pledge===<br /> I, Sarah Lang, have read the Chem/Bioc 430 course syllabus and understand the general structure and expectations of the research program. The above material was prepared after consultation, and in conjunction with my research advisor.</div>

Sarahalang535 http://205.166.159.208/wiki/index.php?title=Lang_Research_2016&diff=4461 2016-12-07T15:26:20Z

<p>Sarahalang535: /* 2. Introduction */</p> <hr /> <div>==Proposed Project Description==<br /> <br /> The goal of this project is to understand the enzymatic oxidation of biophenols--specifically, of eugenol and methyleugenol. In order to collect data on these oxidations, we will use HPLC (High Performance Liquid Chromatography). My focus will also be on comparing new data that I have collected to previous data by [http://esr.monmsci.net/wiki/index.php/Pauzi_Research_Spring_2016 Matt (Amad Pauzi)], who initially worked on this project.<br /> <br /> ===Identifying Products of the Peroxidative Metabolism of Eugenol and Methyleugenol===<br /> <br /> ===1. Descriptive information===<br /> <br /> '''Current Researcher:''' Sarah Lang<br /> '''Faculty Research Advisor:''' Brad Sturgeon<br /> '''Other Student Researchers/Collaborators:''' Ben Stillwell<br /> '''Lab Notebook:''' (Pages)(Title)(Dates)<br /> <br /> ===2. Introduction===<br /> Methyleugenol (MEU) and Eugenol (EUG), both of which are compounds found in clove oil, have been the subject of conflicting safety reports (1). According to the National Extract Manufacturer’s Association, there is no significant carcinogenic risk associated with MEU and EUG. However, a report issued by the National Toxicology Program stated that MEU was “reasonably anticipated to be a human carcinogen,” after observing increased incidences of hepatic cancers in rats after dietary administration of MEU (2). Despite these conclusions, the mechanisms by which MEU and EUG result in carcinogenesis are still largely unknown. <br /> In a study conducted by Herbert J. Sipe et al., MEU and EUG were found to undergo peroxidative metabolism to form free radicals (1). These free radicals, which are highly reactive and transient, have the potential to bind with DNA, form more stable products with each other, or contribute to other forms of cellular damage. Sipe et al. suggest that peroxidase metabolism and the resultant free radicals formed in MEU and EUG contribute to their observed carcinogenicity. However, many of the details of the metabolic processes that occur during the peroxidative metabolism of MEU and EUG still remain to be investigated. In particular, although the existence of free radicals were detected in MEU and EUG, it is unknown how these radicals behave after formation, and whether or not the products formed as a result of these free radicals also contribute to the carcinogenicity of MEU and EUG (1).<br /> In order to mimic peroxidative activity in the liver, horseradish peroxidase may be utilized in order to form free radicals in MEU and EUG. After reactions with horseradish peroxidase, the products can be analyzed through High Performance Liquid Chromatography (HPLC) in order to identify product separation as a result of peroxidative metabolism. Flash Chromatography is used in conjunction with HPLC in order to collect products associated with each peak, and these products can be further characterized using Nuclear Magnetic Resonance spectroscopy (NMR). By isolating the products formed after the metabolic oxidation of MEU and EUG and characterizing these products, it is possible to better understand how their free radicals react to form stable products and what the identities of these products are. Eventually, these products may be tested for carcinogenetic effects themselves, in order to contribute to the understanding of why MEU and EUG result in carcinogenesis.<br /> <br /> ===3. Background from earlier reports===<br /> I have not completed any previous reports to discuss at this time. However, prior research on the oxidation of biophenols, with the aim of eventually studying the oxidation of methyleugenol and eugenol, was conducted by Ahmad Pauzi. The food preservative, BHA, was studied in terms of its enzymatic oxidation. BHA forms free radicals as well, which react with each other in numerous different ways. However, the free radicals are likely to tend towards the most stable products. In the case of BHA, the free radicals react to form dimers. This study’s design was then adapted for my project, where I will study the enxymatic oxidation of methyleugenol and eugenol.<br /> <br /> ===4. Experimental===<br /> <br /> HPLC standards were created using 2 mM MEU/EUG in 10 mL of pH = 7.4 buffer. For the primary run, dioxane was not added to the standards. The standards were then run in the HPLC to identify standard peaks for MEU and EUG. Standards were run through the HPLC for a total of 25 minutes each in order to allow for full elution, as oils have a tendency to adhere to the HPLC column and result in slower elution times. <br /> <br /> ===5. Results===<br /> <br /> Pictured are the standards for MEU and EUG, respectively. Standards were 2 mM MEU/EUG in 10 mL of pH = 7.4 buffer. <br /> <br /> ===6. Discussion===<br /> The HPLC runs of MEU and EUG standards allowed me to become more comfortable with creating reaction mixtures, as well as using the instrumentation for this particular research project. <br /> The standards for MEU and EUG showed peaks that eluted ~22 minutes of the total 25 minutes of run time. This indicates that MEU and EUG, as oils, take longer to elute than other mixtures typically would. In order to address this particular problem, it is suggested that I used a 1:1 ratio of dioxane and buffer rather than 10 mL of buffer only in order to aid in the dissolution of the oils that I am working with. <br /> The standard peaks for MEU and EUG also exhibit some differences. MEU has an additional peak that is not present in EUG, which may be indicative of the differences in their structures (MEU has a dimethoxy group, and EUG only has a single methoxy group). It is also possible that this extra peak present on MEU is due to impurities in the sample, so it will be to my advantage to run the standards again using dioxane and buffer in order to compare results. <br /> <br /> ===7. Conclusions===<br /> No conclusions have been reached at this time. <br /> <br /> ===8. Future Directions===<br /> <br /> Following the creation of standards using 1:1 ratios of dioxane and 10 mL buffer, the next step in this project will be to carry out peroxidase reactions with MEU and EUG and analyze the samples using the HPLC. After identification of the peaks resulting from the peroxidative metabolism of MEU and EUG, these peaks will be collected using Flash Chromatography, in order to analyze the samples using NMR and characterize the products that were formed. Beyond the scope of my time with this project, but in the future as the ultimate goal for understanding the peroxidative metabolism of MEU and EUG, these products will be tested for bioactivity in order to determine whether or not they have carcinogenic effects themselves. <br /> <br /> ===9. Literature references===<br /> <br /> 1. Sipe, H. J., Lardinois, O. M., &amp; Mason, R. P. (2014). Free Radical Metabolism of Methyleugenol and Related Compounds. Chemical Research in Toxicology, 27(4), 483–489. <br /> <br /> 2. Final Report on Carcinogens Background Document for Methyleugenol, Meeting of the NTP Board of Scientific Counselors, Report on Carcinogens Subcommittee, U.S. Department of Health and Human Services, Public Health Service, National Toxicology Program, Research Triangle Park, NC, (2000).<br /> <br /> ===10. Signature===<br /> Sarah Lang 12/7/2016</div>

Sarahalang535 http://205.166.159.208/wiki/index.php?title=Lang_Research_2016&diff=4460 2016-12-07T15:16:57Z

<p>Sarahalang535: /* 2. Introduction */</p> <hr /> <div>==Proposed Project Description==<br /> <br /> The goal of this project is to understand the enzymatic oxidation of biophenols--specifically, of eugenol and methyleugenol. In order to collect data on these oxidations, we will use HPLC (High Performance Liquid Chromatography). My focus will also be on comparing new data that I have collected to previous data by [http://esr.monmsci.net/wiki/index.php/Pauzi_Research_Spring_2016 Matt (Amad Pauzi)], who initially worked on this project.<br /> <br /> ===Identifying Products of the Peroxidative Metabolism of Eugenol and Methyleugenol===<br /> <br /> ===1. Descriptive information===<br /> <br /> '''Current Researcher:''' Sarah Lang<br /> '''Faculty Research Advisor:''' Brad Sturgeon<br /> '''Other Student Researchers/Collaborators:''' Ben Stillwell<br /> '''Lab Notebook:''' (Pages)(Title)(Dates)<br /> <br /> ===2. Introduction===<br /> Methyleugenol (MEU) and Eugenol (EUG), both of which are compounds found in clove oil, have been the subject of conflicting safety reports (1). According to the National Extract Manufacturer’s Association, there is no significant carcinogenic risk associated with MEU and EUG. However, a report issued by the National Toxicology Program stated that MEU was “reasonably anticipated to be a human carcinogen,” after observing increased incidences of hepatic cancers in rats after dietary administration of MEU (2). Despite these conclusions, the mechanisms by which MEU and EUG result in carcinogenesis are still largely unknown. <br /> In a study conducted by Herbert J. Sipe et al., MEU and EUG were found to undergo peroxidative metabolism to form free radicals (1). These free radicals, which are highly reactive and transient, have the potential to bind with DNA, form more stable products with each other, or contribute to other forms of cellular damage. Sipe et al. suggest that peroxidase metabolism and the resultant free radicals formed in MEU and EUG contribute to their observed carcinogenicity. However, many of the details of the metabolic processes that occur during the peroxidative metabolism of MEU and EUG still remain to be investigated. In particular, although the existence of free radicals were detected in MEU and EUG, it is unknown how these radicals behave after formation, and whether or not the products formed as a result of these free radicals also contribute to the carcinogenicity of MEU and EUG (1).<br /> In order to mimic peroxidative activity in the liver, horseradish peroxidase may be utilized in order to form free radicals in MEU and EUG. After reactions with horseradish peroxidase, the products can be analyzed through High Pressure Liquid Chromatography (HPLC) in order to identify product separation as a result of peroxidative metabolism. Flash Chromatography is used in conjunction with HPLC in order to collect products associated with each peak, and these products can be further characterized using Nuclear Magnetic Resonance spectroscopy (NMR). By isolating the products formed after the metabolic oxidation of MEU and EUG and characterizing these products, it is possible to better understand how their free radicals react to form stable products and what the identities of these products are. Eventually, these products may be tested for carcinogenetic effects themselves, in order to contribute to the understanding of why MEU and EUG result in carcinogenesis.<br /> <br /> ===3. Background from earlier reports===<br /> I have not completed any previous reports to discuss at this time. However, prior research on the oxidation of biophenols, with the aim of eventually studying the oxidation of methyleugenol and eugenol, was conducted by Ahmad Pauzi. The food preservative, BHA, was studied in terms of its enzymatic oxidation. BHA forms free radicals as well, which react with each other in numerous different ways. However, the free radicals are likely to tend towards the most stable products. In the case of BHA, the free radicals react to form dimers. This study’s design was then adapted for my project, where I will study the enxymatic oxidation of methyleugenol and eugenol.<br /> <br /> ===4. Experimental===<br /> <br /> HPLC standards were created using 2 mM MEU/EUG in 10 mL of pH = 7.4 buffer. For the primary run, dioxane was not added to the standards. The standards were then run in the HPLC to identify standard peaks for MEU and EUG. Standards were run through the HPLC for a total of 25 minutes each in order to allow for full elution, as oils have a tendency to adhere to the HPLC column and result in slower elution times. <br /> <br /> ===5. Results===<br /> <br /> Pictured are the standards for MEU and EUG, respectively. Standards were 2 mM MEU/EUG in 10 mL of pH = 7.4 buffer. <br /> <br /> ===6. Discussion===<br /> The HPLC runs of MEU and EUG standards allowed me to become more comfortable with creating reaction mixtures, as well as using the instrumentation for this particular research project. <br /> The standards for MEU and EUG showed peaks that eluted ~22 minutes of the total 25 minutes of run time. This indicates that MEU and EUG, as oils, take longer to elute than other mixtures typically would. In order to address this particular problem, it is suggested that I used a 1:1 ratio of dioxane and buffer rather than 10 mL of buffer only in order to aid in the dissolution of the oils that I am working with. <br /> The standard peaks for MEU and EUG also exhibit some differences. MEU has an additional peak that is not present in EUG, which may be indicative of the differences in their structures (MEU has a dimethoxy group, and EUG only has a single methoxy group). It is also possible that this extra peak present on MEU is due to impurities in the sample, so it will be to my advantage to run the standards again using dioxane and buffer in order to compare results. <br /> <br /> ===7. Conclusions===<br /> No conclusions have been reached at this time. <br /> <br /> ===8. Future Directions===<br /> <br /> Following the creation of standards using 1:1 ratios of dioxane and 10 mL buffer, the next step in this project will be to carry out peroxidase reactions with MEU and EUG and analyze the samples using the HPLC. After identification of the peaks resulting from the peroxidative metabolism of MEU and EUG, these peaks will be collected using Flash Chromatography, in order to analyze the samples using NMR and characterize the products that were formed. Beyond the scope of my time with this project, but in the future as the ultimate goal for understanding the peroxidative metabolism of MEU and EUG, these products will be tested for bioactivity in order to determine whether or not they have carcinogenic effects themselves. <br /> <br /> ===9. Literature references===<br /> <br /> 1. Sipe, H. J., Lardinois, O. M., &amp; Mason, R. P. (2014). Free Radical Metabolism of Methyleugenol and Related Compounds. Chemical Research in Toxicology, 27(4), 483–489. <br /> <br /> 2. Final Report on Carcinogens Background Document for Methyleugenol, Meeting of the NTP Board of Scientific Counselors, Report on Carcinogens Subcommittee, U.S. Department of Health and Human Services, Public Health Service, National Toxicology Program, Research Triangle Park, NC, (2000).<br /> <br /> ===10. Signature===<br /> Sarah Lang 12/7/2016</div>

Sarahalang535 http://205.166.159.208/wiki/index.php?title=Lang_Research_2016&diff=4459 2016-12-07T15:15:45Z

<p>Sarahalang535: </p> <hr /> <div>==Proposed Project Description==<br /> <br /> The goal of this project is to understand the enzymatic oxidation of biophenols--specifically, of eugenol and methyleugenol. In order to collect data on these oxidations, we will use HPLC (High Performance Liquid Chromatography). My focus will also be on comparing new data that I have collected to previous data by [http://esr.monmsci.net/wiki/index.php/Pauzi_Research_Spring_2016 Matt (Amad Pauzi)], who initially worked on this project.<br /> <br /> ===Identifying Products of the Peroxidative Metabolism of Eugenol and Methyleugenol===<br /> <br /> ===1. Descriptive information===<br /> <br /> '''Current Researcher:''' Sarah Lang<br /> '''Faculty Research Advisor:''' Brad Sturgeon<br /> '''Other Student Researchers/Collaborators:''' Ben Stillwell<br /> '''Lab Notebook:''' (Pages)(Title)(Dates)<br /> <br /> ===2. Introduction===<br /> Methyleugenol (MEU) and Eugenol (EUG), both of which are compounds found in clove oil, have been the subject of conflicting safety reports1. According to the National Extract Manufacturer’s Association, there is no significant carcinogenic risk associated with MEU and EUG. However, a report issued by the National Toxicology Program stated that MEU was “reasonably anticipated to be a human carcinogen,” after observing increased incidences of hepatic cancers in rats after dietary administration of MEU2. Despite these conclusions, the mechanisms by which MEU and EUG result in carcinogenesis are still largely unknown. <br /> In a study conducted by Herbert J. Sipe et al., MEU and EUG were found to undergo peroxidative metabolism to form free radicals1. These free radicals, which are highly reactive and transient, have the potential to bind with DNA, form more stable products with each other, or contribute to other forms of cellular damage. Sipe et al. suggest that peroxidase metabolism and the resultant free radicals formed in MEU and EUG contribute to their observed carcinogenicity. However, many of the details of the metabolic processes that occur during the peroxidative metabolism of MEU and EUG still remain to be investigated. In particular, although the existence of free radicals were detected in MEU and EUG, it is unknown how these radicals behave after formation, and whether or not the products formed as a result of these free radicals also contribute to the carcinogenicity of MEU and EUG1.<br /> In order to mimic peroxidative activity in the liver, horseradish peroxidase may be utilized in order to form free radicals in MEU and EUG. After reactions with horseradish peroxidase, the products can be analyzed through High Pressure Liquid Chromatography (HPLC) in order to identify product separation as a result of peroxidative metabolism. Flash Chromatography is used in conjunction with HPLC in order to collect products associated with each peak, and these products can be further characterized using Nuclear Magnetic Resonance spectroscopy (NMR). By isolating the products formed after the metabolic oxidation of MEU and EUG and characterizing these products, it is possible to better understand how their free radicals react to form stable products and what the identities of these products are. Eventually, these products may be tested for carcinogenetic effects themselves, in order to contribute to the understanding of why MEU and EUG result in carcinogenesis. <br /> <br /> ===3. Background from earlier reports===<br /> I have not completed any previous reports to discuss at this time. However, prior research on the oxidation of biophenols, with the aim of eventually studying the oxidation of methyleugenol and eugenol, was conducted by Ahmad Pauzi. The food preservative, BHA, was studied in terms of its enzymatic oxidation. BHA forms free radicals as well, which react with each other in numerous different ways. However, the free radicals are likely to tend towards the most stable products. In the case of BHA, the free radicals react to form dimers. This study’s design was then adapted for my project, where I will study the enxymatic oxidation of methyleugenol and eugenol.<br /> <br /> ===4. Experimental===<br /> <br /> HPLC standards were created using 2 mM MEU/EUG in 10 mL of pH = 7.4 buffer. For the primary run, dioxane was not added to the standards. The standards were then run in the HPLC to identify standard peaks for MEU and EUG. Standards were run through the HPLC for a total of 25 minutes each in order to allow for full elution, as oils have a tendency to adhere to the HPLC column and result in slower elution times. <br /> <br /> ===5. Results===<br /> <br /> Pictured are the standards for MEU and EUG, respectively. Standards were 2 mM MEU/EUG in 10 mL of pH = 7.4 buffer. <br /> <br /> ===6. Discussion===<br /> The HPLC runs of MEU and EUG standards allowed me to become more comfortable with creating reaction mixtures, as well as using the instrumentation for this particular research project. <br /> The standards for MEU and EUG showed peaks that eluted ~22 minutes of the total 25 minutes of run time. This indicates that MEU and EUG, as oils, take longer to elute than other mixtures typically would. In order to address this particular problem, it is suggested that I used a 1:1 ratio of dioxane and buffer rather than 10 mL of buffer only in order to aid in the dissolution of the oils that I am working with. <br /> The standard peaks for MEU and EUG also exhibit some differences. MEU has an additional peak that is not present in EUG, which may be indicative of the differences in their structures (MEU has a dimethoxy group, and EUG only has a single methoxy group). It is also possible that this extra peak present on MEU is due to impurities in the sample, so it will be to my advantage to run the standards again using dioxane and buffer in order to compare results. <br /> <br /> ===7. Conclusions===<br /> No conclusions have been reached at this time. <br /> <br /> ===8. Future Directions===<br /> <br /> Following the creation of standards using 1:1 ratios of dioxane and 10 mL buffer, the next step in this project will be to carry out peroxidase reactions with MEU and EUG and analyze the samples using the HPLC. After identification of the peaks resulting from the peroxidative metabolism of MEU and EUG, these peaks will be collected using Flash Chromatography, in order to analyze the samples using NMR and characterize the products that were formed. Beyond the scope of my time with this project, but in the future as the ultimate goal for understanding the peroxidative metabolism of MEU and EUG, these products will be tested for bioactivity in order to determine whether or not they have carcinogenic effects themselves. <br /> <br /> ===9. Literature references===<br /> <br /> 1. Sipe, H. J., Lardinois, O. M., &amp; Mason, R. P. (2014). Free Radical Metabolism of Methyleugenol and Related Compounds. Chemical Research in Toxicology, 27(4), 483–489. <br /> <br /> 2. Final Report on Carcinogens Background Document for Methyleugenol, Meeting of the NTP Board of Scientific Counselors, Report on Carcinogens Subcommittee, U.S. Department of Health and Human Services, Public Health Service, National Toxicology Program, Research Triangle Park, NC, (2000).<br /> <br /> ===10. Signature===<br /> Sarah Lang 12/7/2016</div>

Sarahalang535 http://205.166.159.208/wiki/index.php?title=Ice_Water_Thermal_Imaging&diff=3370 2016-09-22T22:23:57Z

<p>Sarahalang535: </p> <hr /> <div>[[File:IRfusion.png]]<br /> <br /> This image shows a beaker full of ice water (appears black with a purple outline), with my hand held above after being submerged in the water for &lt;1 second. In the image, my fingers are no longer detected by the camera, because they have cooled to the point where they no longer give off enough heat to be detected by the camera when compared with the maximum heat given off by the part of my hand that was not submerged.</div>

Sarahalang535 http://205.166.159.208/wiki/index.php?title=Ice_Water_Thermal_Imaging&diff=3357 2016-09-22T22:22:21Z

<p>Sarahalang535: Created page with &quot;File:IRfusion.png This image shows a beaker full of ice water (appears black with a purple outline), with my hand held above after being submerged in the water for &lt;1 sec...&quot;</p> <hr /> <div>[[File:IRfusion.png]]<br /> <br /> This image shows a beaker full of ice water (appears black with a purple outline), with my hand held above after being submerged in the water for &lt;1 second. In the image, my fingers are no longer detected by IR imaging, because they have cooled to the point where they no longer appear in the spectra. The rest of my arm is visible, and appears in the image as red to green around the edges.</div>

Sarahalang535 http://205.166.159.208/wiki/index.php?title=File:IRfusion.png&diff=3322 2016-09-22T22:09:53Z

<p>Sarahalang535: </p> <hr /> <div></div>

Sarahalang535 http://205.166.159.208/wiki/index.php?title=File:IRfusion.bmp&diff=3310 2016-09-22T22:04:19Z

<p>Sarahalang535: Sarahalang535 uploaded a new version of File:IRfusion.bmp</p> <hr /> <div></div>

Sarahalang535 http://205.166.159.208/wiki/index.php?title=File:IRfusion.bmp&diff=3308 2016-09-22T22:03:41Z

<p>Sarahalang535: </p> <hr /> <div></div>

Sarahalang535 http://205.166.159.208/wiki/index.php?title=Sarah_Lang_Chem430_F16&diff=3296 2016-09-22T17:21:33Z

<p>Sarahalang535: /* Instruments to be used */</p> <hr /> <div>Chemistry/Biochemistry Research 430<br /> :Fall 2016<br /> :Sarah Lang<br /> :Senior Biochemistry Major<br /> <br /> ==Research Times==<br /> Mon 2:00-6:00pm<br /> :section 01 = 0.25 credit = 4 hours per week.<br /> <br /> ==Proposed Research Project==<br /> ===The Enzymatic Oxidation of Eugenol and Methyleugenol===<br /> <br /> ===General Information===<br /> :Advisor: Brad Sturgeon<br /> :Other research student collaborators: <br /> :Other Research Collaborators:<br /> <br /> ===Proposal===<br /> The purpose of this project is to understand the enzymatic oxidation of biophenols--specifically, of eugenol and methyleugenol. Both eugenol and methyleugenol are commonly found in flavoring components of foods, and recently, connections have been made between methyleugenol (and to a lesser extent, eugenol) and increased incidence of malignant tumors at various tissue sites (''1''). Furthermore, studies have shown that both eugenol and methyleugenol undergo peroxidative metabolism to form free radicals, but little is known about the individual products of this process. The goal of this research project is to identify, collect, and separate the products formed during the oxidation of eugenol and methyleugenol, in order to eventually gain a better understanding of their biological activity.<br /> <br /> ===Instruments to be used===<br /> <br /> HPLC, <br /> Flash Chromotography, <br /> NMR<br /> <br /> ===References (2 minimum)===<br /> 1. Sipe, H. J., Lardinois, O. M., &amp; Mason, R. P. (2014). Free Radical Metabolism of Methyleugenol and Related Compounds. Chemical Research in Toxicology, 27(4), 483–489. http://doi.org/10.1021/tx400256b<br /> <br /> 2. Mastelić, J., Jerković, I., Blažević, I., Poljak-Blaži, M., Borović, S., Ivančić-Baće, I., … Müller, N. (2008). Comparative Study on the Antioxidant and Biological Activities of Carvacrol, Thymol, and Eugenol Derivatives. Journal of Agricultural and Food Chemistry, 56(11), 3989–3996. http://doi.org/10.1021/jf073272v<br /> <br /> ===Research pledge===<br /> I, Sarah Lang, have read the Chem/Bioc 430 course syllabus and understand the general structure and expectations of the research program. The above material was prepared after consultation, and in conjunction with my research advisor.</div>

Sarahalang535 http://205.166.159.208/wiki/index.php?title=Sarah_Lang_Chem430_F16&diff=3295 2016-09-22T17:21:21Z

<p>Sarahalang535: /* Instruments to be used */</p> <hr /> <div>Chemistry/Biochemistry Research 430<br /> :Fall 2016<br /> :Sarah Lang<br /> :Senior Biochemistry Major<br /> <br /> ==Research Times==<br /> Mon 2:00-6:00pm<br /> :section 01 = 0.25 credit = 4 hours per week.<br /> <br /> ==Proposed Research Project==<br /> ===The Enzymatic Oxidation of Eugenol and Methyleugenol===<br /> <br /> ===General Information===<br /> :Advisor: Brad Sturgeon<br /> :Other research student collaborators: <br /> :Other Research Collaborators:<br /> <br /> ===Proposal===<br /> The purpose of this project is to understand the enzymatic oxidation of biophenols--specifically, of eugenol and methyleugenol. Both eugenol and methyleugenol are commonly found in flavoring components of foods, and recently, connections have been made between methyleugenol (and to a lesser extent, eugenol) and increased incidence of malignant tumors at various tissue sites (''1''). Furthermore, studies have shown that both eugenol and methyleugenol undergo peroxidative metabolism to form free radicals, but little is known about the individual products of this process. The goal of this research project is to identify, collect, and separate the products formed during the oxidation of eugenol and methyleugenol, in order to eventually gain a better understanding of their biological activity.<br /> <br /> ===Instruments to be used===<br /> <br /> HPLC <br /> Flash Chromotography <br /> NMR<br /> <br /> ===References (2 minimum)===<br /> 1. Sipe, H. J., Lardinois, O. M., &amp; Mason, R. P. (2014). Free Radical Metabolism of Methyleugenol and Related Compounds. Chemical Research in Toxicology, 27(4), 483–489. http://doi.org/10.1021/tx400256b<br /> <br /> 2. Mastelić, J., Jerković, I., Blažević, I., Poljak-Blaži, M., Borović, S., Ivančić-Baće, I., … Müller, N. (2008). Comparative Study on the Antioxidant and Biological Activities of Carvacrol, Thymol, and Eugenol Derivatives. Journal of Agricultural and Food Chemistry, 56(11), 3989–3996. http://doi.org/10.1021/jf073272v<br /> <br /> ===Research pledge===<br /> I, Sarah Lang, have read the Chem/Bioc 430 course syllabus and understand the general structure and expectations of the research program. The above material was prepared after consultation, and in conjunction with my research advisor.</div>

Sarahalang535 http://205.166.159.208/wiki/index.php?title=Sarah_Lang_Chem430_F16&diff=3294 2016-09-22T17:18:50Z

<p>Sarahalang535: /* Proposal */</p> <hr /> <div>Chemistry/Biochemistry Research 430<br /> :Fall 2016<br /> :Sarah Lang<br /> :Senior Biochemistry Major<br /> <br /> ==Research Times==<br /> Mon 2:00-6:00pm<br /> :section 01 = 0.25 credit = 4 hours per week.<br /> <br /> ==Proposed Research Project==<br /> ===The Enzymatic Oxidation of Eugenol and Methyleugenol===<br /> <br /> ===General Information===<br /> :Advisor: Brad Sturgeon<br /> :Other research student collaborators: <br /> :Other Research Collaborators:<br /> <br /> ===Proposal===<br /> The purpose of this project is to understand the enzymatic oxidation of biophenols--specifically, of eugenol and methyleugenol. Both eugenol and methyleugenol are commonly found in flavoring components of foods, and recently, connections have been made between methyleugenol (and to a lesser extent, eugenol) and increased incidence of malignant tumors at various tissue sites (''1''). Furthermore, studies have shown that both eugenol and methyleugenol undergo peroxidative metabolism to form free radicals, but little is known about the individual products of this process. The goal of this research project is to identify, collect, and separate the products formed during the oxidation of eugenol and methyleugenol, in order to eventually gain a better understanding of their biological activity.<br /> <br /> ===Instruments to be used===<br /> <br /> HPLC<br /> <br /> ===References (2 minimum)===<br /> 1. Sipe, H. J., Lardinois, O. M., &amp; Mason, R. P. (2014). Free Radical Metabolism of Methyleugenol and Related Compounds. Chemical Research in Toxicology, 27(4), 483–489. http://doi.org/10.1021/tx400256b<br /> <br /> 2. Mastelić, J., Jerković, I., Blažević, I., Poljak-Blaži, M., Borović, S., Ivančić-Baće, I., … Müller, N. (2008). Comparative Study on the Antioxidant and Biological Activities of Carvacrol, Thymol, and Eugenol Derivatives. Journal of Agricultural and Food Chemistry, 56(11), 3989–3996. http://doi.org/10.1021/jf073272v<br /> <br /> ===Research pledge===<br /> I, Sarah Lang, have read the Chem/Bioc 430 course syllabus and understand the general structure and expectations of the research program. The above material was prepared after consultation, and in conjunction with my research advisor.</div>

Sarahalang535 http://205.166.159.208/wiki/index.php?title=Sarah_Lang_Chem430_F16&diff=3202 2016-09-15T23:10:32Z

<p>Sarahalang535: /* Instruments to be used */</p> <hr /> <div>Chemistry/Biochemistry Research 430<br /> :Fall 2016<br /> :Sarah Lang<br /> :Senior Biochemistry Major<br /> <br /> ==Research Times==<br /> Mon 2:00-6:00pm<br /> :section 01 = 0.25 credit = 4 hours per week.<br /> <br /> ==Proposed Research Project==<br /> ===The Enzymatic Oxidation of Eugenol and Methyleugenol===<br /> <br /> ===General Information===<br /> :Advisor: Brad Sturgeon<br /> :Other research student collaborators: <br /> :Other Research Collaborators:<br /> <br /> ===Proposal===<br /> The purpose of this project is to understand the enzymatic oxidation of biophenols--specifically, of eugenol and methyleugenol. Both eugenol and methyleugenol are commonly found in flavoring components of foods, and recently, connections have been made between methyleugenol (and to a lesser extent, eugenol) and increased incidence of malignant tumors at various tissue sites (''1''). Furthermore, studies have shown that both eugenol and methyleugenol undergo peroxidative metabolism to form free radicals, but little is known about the details of the mechanism behind this oxidation. This project will be focused on gathering data that will help to provide understanding on this particular mechanism.<br /> <br /> ===Instruments to be used===<br /> <br /> HPLC<br /> <br /> ===References (2 minimum)===<br /> 1. Sipe, H. J., Lardinois, O. M., &amp; Mason, R. P. (2014). Free Radical Metabolism of Methyleugenol and Related Compounds. Chemical Research in Toxicology, 27(4), 483–489. http://doi.org/10.1021/tx400256b<br /> <br /> 2. Mastelić, J., Jerković, I., Blažević, I., Poljak-Blaži, M., Borović, S., Ivančić-Baće, I., … Müller, N. (2008). Comparative Study on the Antioxidant and Biological Activities of Carvacrol, Thymol, and Eugenol Derivatives. Journal of Agricultural and Food Chemistry, 56(11), 3989–3996. http://doi.org/10.1021/jf073272v<br /> <br /> ===Research pledge===<br /> I, Sarah Lang, have read the Chem/Bioc 430 course syllabus and understand the general structure and expectations of the research program. The above material was prepared after consultation, and in conjunction with my research advisor.</div>

Sarahalang535 http://205.166.159.208/wiki/index.php?title=Sarah_Lang_Chem430_F16&diff=3201 2016-09-15T23:08:50Z

<p>Sarahalang535: /* Instruments to be used */</p> <hr /> <div>Chemistry/Biochemistry Research 430<br /> :Fall 2016<br /> :Sarah Lang<br /> :Senior Biochemistry Major<br /> <br /> ==Research Times==<br /> Mon 2:00-6:00pm<br /> :section 01 = 0.25 credit = 4 hours per week.<br /> <br /> ==Proposed Research Project==<br /> ===The Enzymatic Oxidation of Eugenol and Methyleugenol===<br /> <br /> ===General Information===<br /> :Advisor: Brad Sturgeon<br /> :Other research student collaborators: <br /> :Other Research Collaborators:<br /> <br /> ===Proposal===<br /> The purpose of this project is to understand the enzymatic oxidation of biophenols--specifically, of eugenol and methyleugenol. Both eugenol and methyleugenol are commonly found in flavoring components of foods, and recently, connections have been made between methyleugenol (and to a lesser extent, eugenol) and increased incidence of malignant tumors at various tissue sites (''1''). Furthermore, studies have shown that both eugenol and methyleugenol undergo peroxidative metabolism to form free radicals, but little is known about the details of the mechanism behind this oxidation. This project will be focused on gathering data that will help to provide understanding on this particular mechanism.<br /> <br /> ===Instruments to be used===<br /> <br /> ===References (2 minimum)===<br /> 1. Sipe, H. J., Lardinois, O. M., &amp; Mason, R. P. (2014). Free Radical Metabolism of Methyleugenol and Related Compounds. Chemical Research in Toxicology, 27(4), 483–489. http://doi.org/10.1021/tx400256b<br /> <br /> 2. Mastelić, J., Jerković, I., Blažević, I., Poljak-Blaži, M., Borović, S., Ivančić-Baće, I., … Müller, N. (2008). Comparative Study on the Antioxidant and Biological Activities of Carvacrol, Thymol, and Eugenol Derivatives. Journal of Agricultural and Food Chemistry, 56(11), 3989–3996. http://doi.org/10.1021/jf073272v<br /> <br /> ===Research pledge===<br /> I, Sarah Lang, have read the Chem/Bioc 430 course syllabus and understand the general structure and expectations of the research program. The above material was prepared after consultation, and in conjunction with my research advisor.</div>

Sarahalang535 http://205.166.159.208/wiki/index.php?title=Sarah_Lang_Chem430_F16&diff=3200 2016-09-15T23:08:27Z

<p>Sarahalang535: /* Proposal */</p> <hr /> <div>Chemistry/Biochemistry Research 430<br /> :Fall 2016<br /> :Sarah Lang<br /> :Senior Biochemistry Major<br /> <br /> ==Research Times==<br /> Mon 2:00-6:00pm<br /> :section 01 = 0.25 credit = 4 hours per week.<br /> <br /> ==Proposed Research Project==<br /> ===The Enzymatic Oxidation of Eugenol and Methyleugenol===<br /> <br /> ===General Information===<br /> :Advisor: Brad Sturgeon<br /> :Other research student collaborators: <br /> :Other Research Collaborators:<br /> <br /> ===Proposal===<br /> The purpose of this project is to understand the enzymatic oxidation of biophenols--specifically, of eugenol and methyleugenol. Both eugenol and methyleugenol are commonly found in flavoring components of foods, and recently, connections have been made between methyleugenol (and to a lesser extent, eugenol) and increased incidence of malignant tumors at various tissue sites (''1''). Furthermore, studies have shown that both eugenol and methyleugenol undergo peroxidative metabolism to form free radicals, but little is known about the details of the mechanism behind this oxidation. This project will be focused on gathering data that will help to provide understanding on this particular mechanism.<br /> <br /> ===Instruments to be used===<br /> <br /> HPLC<br /> <br /> ===References (2 minimum)===<br /> 1. Sipe, H. J., Lardinois, O. M., &amp; Mason, R. P. (2014). Free Radical Metabolism of Methyleugenol and Related Compounds. Chemical Research in Toxicology, 27(4), 483–489. http://doi.org/10.1021/tx400256b<br /> <br /> 2. Mastelić, J., Jerković, I., Blažević, I., Poljak-Blaži, M., Borović, S., Ivančić-Baće, I., … Müller, N. (2008). Comparative Study on the Antioxidant and Biological Activities of Carvacrol, Thymol, and Eugenol Derivatives. Journal of Agricultural and Food Chemistry, 56(11), 3989–3996. http://doi.org/10.1021/jf073272v<br /> <br /> ===Research pledge===<br /> I, Sarah Lang, have read the Chem/Bioc 430 course syllabus and understand the general structure and expectations of the research program. The above material was prepared after consultation, and in conjunction with my research advisor.</div>

Sarahalang535 http://205.166.159.208/wiki/index.php?title=Sarah_Lang_Chem430_F16&diff=3199 2016-09-15T23:08:12Z

<p>Sarahalang535: /* Proposal */</p> <hr /> <div>Chemistry/Biochemistry Research 430<br /> :Fall 2016<br /> :Sarah Lang<br /> :Senior Biochemistry Major<br /> <br /> ==Research Times==<br /> Mon 2:00-6:00pm<br /> :section 01 = 0.25 credit = 4 hours per week.<br /> <br /> ==Proposed Research Project==<br /> ===The Enzymatic Oxidation of Eugenol and Methyleugenol===<br /> <br /> ===General Information===<br /> :Advisor: Brad Sturgeon<br /> :Other research student collaborators: <br /> :Other Research Collaborators:<br /> <br /> ===Proposal===<br /> The purpose of this project is to understand the enzymatic oxidation of biophenols--specifically, of eugenol and methyleugenol. Both eugenol and methyleugenol are commonly found in flavoring components of foods, and recently, connections have been made between methyleugenol (and to a lesser extent, eugenol) and increased incidence of malignant tumors at various tissue sites (''1''). Furthermore, studies have shown that both eugenol and methyleugenol undergo peroxidative metabolism to form free radicals, but little is known about the details of the mechanism behind this oxidation. This project will be focused on gathering data that will help provide understanding on this particular mechanism.<br /> <br /> ===Instruments to be used===<br /> <br /> HPLC<br /> <br /> ===References (2 minimum)===<br /> 1. Sipe, H. J., Lardinois, O. M., &amp; Mason, R. P. (2014). Free Radical Metabolism of Methyleugenol and Related Compounds. Chemical Research in Toxicology, 27(4), 483–489. http://doi.org/10.1021/tx400256b<br /> <br /> 2. Mastelić, J., Jerković, I., Blažević, I., Poljak-Blaži, M., Borović, S., Ivančić-Baće, I., … Müller, N. (2008). Comparative Study on the Antioxidant and Biological Activities of Carvacrol, Thymol, and Eugenol Derivatives. Journal of Agricultural and Food Chemistry, 56(11), 3989–3996. http://doi.org/10.1021/jf073272v<br /> <br /> ===Research pledge===<br /> I, Sarah Lang, have read the Chem/Bioc 430 course syllabus and understand the general structure and expectations of the research program. The above material was prepared after consultation, and in conjunction with my research advisor.</div>

Sarahalang535 http://205.166.159.208/wiki/index.php?title=Sarah_Lang&diff=3076 2016-09-07T04:34:14Z

<p>Sarahalang535: /* Other Involvement */</p> <hr /> <div>&lt;!-- Greetings...this text does not show up on the page since it is &quot;hidden&quot; with the surrounding tags --&gt;<br /> &lt;!-- This is the suggested format for current students to organize their information; this is NOT a requirement --&gt;<br /> &lt;!-- To add this structure to your personal wiki page, copy and paste the code below and then remove the &quot;hidden&quot; tags --&gt;<br /> &lt;!-- ...then of course...add your information. Use Mr. Bitchly's text for coding examples...pls add examples to this profile --&gt;<br /> <br /> &lt;!-- ==Personal Information== --&gt;<br /> &lt;!-- ==Undergraduate Research Activities== --&gt;<br /> &lt;!-- ==Presentations== --&gt;<br /> &lt;!-- ==Interests== --&gt;<br /> &lt;!-- ==Career Goals== --&gt;<br /> &lt;!-- --&gt;<br /> &lt;!-- --&gt;<br /> <br /> ==Personal Information==<br /> Senior biochemistry major<br /> <br /> Hometown: Peoria, IL<br /> <br /> Contact Info: 309-265-1518; slang@monmouthcollege.edu<br /> <br /> ==Undergraduate Research Activities==<br /> <br /> Aug 2013: SOfIA (2013) The Effects of Sulfadimethoxine and Malachite Green on the Gregarine Parasite Count of Madagascar Hissing Cockroaches<br /> *Studied how different dosages of two drugs, sulfadimethoxine and malachite green, affected the level of gregarine parasite infection in Madagascar Hissing Cockroaches<br /> *Constructed presentation and poster to present findings at the end of the 3-week research period<br /> <br /> Aug 2015: SOfIA (2015) The Effects of Gregarine Parasites on the Physiology of Madagascar Hissing Cockroaches<br /> *Guided freshman SOfIA students on project creation and served as a mentor throughout the project<br /> *Aided in new students' development of lab, research, and presentation skills<br /> <br /> Current: BIOC 430 with Sturgeon<br /> <br /> ==Presentations==<br /> Aug 2013: (SOfIA poster presentation)''The Effects of Sulfadimethoxine and Malachite Green on the Gregarine Parasite Count of Madagascar Hissing Cockroaches''<br /> <br /> Aug 2015: (SOfIA poster presentation)''The Effects of Gregarine Parasites on the Physiology of Madagascar Hissing Cockroaches''<br /> <br /> ==Other Involvement==<br /> <br /> '''Midwest Journal of Undergraduate Research'''<br /> *Publications Editor: responsible for formatting the final printed journal using InDesign software<br /> *Developed skills in reviewing and editing undergraduate research papers from a variety of disciplines<br /> <br /> '''Teaching Assistant'''<br /> *Managed lab safety and aided students in proper use of instrumentation<br /> *Provided feedback to students on lab calculations and lab technique<br /> <br /> '''Biology 150 SI'''<br /> *Held sessions outside of regular class time to help students understand a variety of topics<br /> *Organized evening review sessions for exams<br /> *Worked with the Biology 150 professor to construct and distribute study guides for each in-class exam<br /> <br /> '''OSF Summer Student Volunteer'''<br /> *30+ hours spent working in the Adult Medical/Surgical Unit<br /> *Aided in replacing shipments of medical supplies, maintaining sanitization, and ensuring patient comfort whenever possible<br /> *Developed skills in bedside manner, recognition of necessary equipment, and hospital navigation.<br /> <br /> '''Monmouth College Swim Team (2013-2015)'''<br /> *Academic All-Conference Athlete (2013-2015)<br /> *Developed skills in both leading and cooperating on a diverse team of athletes<br /> <br /> ==Career Plans==<br /> <br /> Nurse Practitioner (through direct-entry MSN for non-nursing majors)</div>

Sarahalang535 http://205.166.159.208/wiki/index.php?title=Sarah_Lang_Chem430_F16&diff=3075 2016-09-07T04:31:53Z

<p>Sarahalang535: /* Proposal */</p> <hr /> <div>Chemistry/Biochemistry Research 430<br /> :Fall 2016<br /> :Sarah Lang<br /> :Senior Biochemistry Major<br /> <br /> ==Research Times==<br /> Mon 2:00-6:00pm<br /> :section 01 = 0.25 credit = 4 hours per week.<br /> <br /> ==Proposed Research Project==<br /> ===The Enzymatic Oxidation of Eugenol and Methyleugenol===<br /> <br /> ===General Information===<br /> :Advisor: Brad Sturgeon<br /> :Other research student collaborators: <br /> :Other Research Collaborators:<br /> <br /> ===Proposal===<br /> The purpose of this project is to understand the enzymatic oxidation of biophenols--specifically, of eugenol and methyleugenol. Both eugenol and methyleugenol are commonly found in flavoring components of foods, and recently, connections have been made between methyleugenol (and to a lesser extent, eugenol) and increased incidence of malignant tumors at various tissue sites (''1''). Furthermore, studies have shown that both eugenol and methyleugenol undergo peroxidative metabolism to form free radicals, but little is known about the details of the mechanism behind this oxidation. My focus will be on gathering data that will help us to understand this particular mechanism.<br /> <br /> ===Instruments to be used===<br /> <br /> HPLC<br /> <br /> ===References (2 minimum)===<br /> 1. Sipe, H. J., Lardinois, O. M., &amp; Mason, R. P. (2014). Free Radical Metabolism of Methyleugenol and Related Compounds. Chemical Research in Toxicology, 27(4), 483–489. http://doi.org/10.1021/tx400256b<br /> <br /> 2. Mastelić, J., Jerković, I., Blažević, I., Poljak-Blaži, M., Borović, S., Ivančić-Baće, I., … Müller, N. (2008). Comparative Study on the Antioxidant and Biological Activities of Carvacrol, Thymol, and Eugenol Derivatives. Journal of Agricultural and Food Chemistry, 56(11), 3989–3996. http://doi.org/10.1021/jf073272v<br /> <br /> ===Research pledge===<br /> I, Sarah Lang, have read the Chem/Bioc 430 course syllabus and understand the general structure and expectations of the research program. The above material was prepared after consultation, and in conjunction with my research advisor.</div>

Sarahalang535 http://205.166.159.208/wiki/index.php?title=Sarah_Lang_Chem430_F16&diff=3074 2016-09-07T04:31:41Z

<p>Sarahalang535: /* Proposal */</p> <hr /> <div>Chemistry/Biochemistry Research 430<br /> :Fall 2016<br /> :Sarah Lang<br /> :Senior Biochemistry Major<br /> <br /> ==Research Times==<br /> Mon 2:00-6:00pm<br /> :section 01 = 0.25 credit = 4 hours per week.<br /> <br /> ==Proposed Research Project==<br /> ===The Enzymatic Oxidation of Eugenol and Methyleugenol===<br /> <br /> ===General Information===<br /> :Advisor: Brad Sturgeon<br /> :Other research student collaborators: <br /> :Other Research Collaborators:<br /> <br /> ===Proposal===<br /> The purpose of this project is to understand the enzymatic oxidation of biophenols--specifically, of eugenol and methyleugenol. Both eugenol and methyleugenol are commonly found in flavoring components of foods, and recently, connections have been made between methyleugenol (and to a lesser extent, eugenol) and increased incidence of malignant tumors at various tissue sites (''1''). Furthermore, studies have shown that both eugenol and methyleugenol undergo peroxidative metabolism to form free radicals, but little is known about the details of the mechanism behind this oxidation. My focus will be on gathering data that will help us understand this particular mechanism.<br /> <br /> ===Instruments to be used===<br /> <br /> HPLC<br /> <br /> ===References (2 minimum)===<br /> 1. Sipe, H. J., Lardinois, O. M., &amp; Mason, R. P. (2014). Free Radical Metabolism of Methyleugenol and Related Compounds. Chemical Research in Toxicology, 27(4), 483–489. http://doi.org/10.1021/tx400256b<br /> <br /> 2. Mastelić, J., Jerković, I., Blažević, I., Poljak-Blaži, M., Borović, S., Ivančić-Baće, I., … Müller, N. (2008). Comparative Study on the Antioxidant and Biological Activities of Carvacrol, Thymol, and Eugenol Derivatives. Journal of Agricultural and Food Chemistry, 56(11), 3989–3996. http://doi.org/10.1021/jf073272v<br /> <br /> ===Research pledge===<br /> I, Sarah Lang, have read the Chem/Bioc 430 course syllabus and understand the general structure and expectations of the research program. The above material was prepared after consultation, and in conjunction with my research advisor.</div>

Sarahalang535 http://205.166.159.208/wiki/index.php?title=Sarah_Lang_Chem430_F16&diff=3073 2016-09-07T04:30:31Z

<p>Sarahalang535: /* Proposal */</p> <hr /> <div>Chemistry/Biochemistry Research 430<br /> :Fall 2016<br /> :Sarah Lang<br /> :Senior Biochemistry Major<br /> <br /> ==Research Times==<br /> Mon 2:00-6:00pm<br /> :section 01 = 0.25 credit = 4 hours per week.<br /> <br /> ==Proposed Research Project==<br /> ===The Enzymatic Oxidation of Eugenol and Methyleugenol===<br /> <br /> ===General Information===<br /> :Advisor: Brad Sturgeon<br /> :Other research student collaborators: <br /> :Other Research Collaborators:<br /> <br /> ===Proposal===<br /> The purpose of this project is to understand the enzymatic oxidation of biophenols--specifically, of eugenol and methyleugenol. Both eugenol and methyleugenol are commonly found in flavoring components of foods, and recently, connections have been made between methyleugenol (and to a lesser extent, eugenol) and increased incidence of malignant tumors at various tissue sites (''1''). Furthermore, studies have shown that both eugenol and methyleugenol undergo peroxidative metabolism to form free radicals, but little is known about the details of the mechanism behind this oxidation. My focus will be on gathering data on this particular mechanism.<br /> <br /> ===Instruments to be used===<br /> <br /> HPLC<br /> <br /> ===References (2 minimum)===<br /> 1. Sipe, H. J., Lardinois, O. M., &amp; Mason, R. P. (2014). Free Radical Metabolism of Methyleugenol and Related Compounds. Chemical Research in Toxicology, 27(4), 483–489. http://doi.org/10.1021/tx400256b<br /> <br /> 2. Mastelić, J., Jerković, I., Blažević, I., Poljak-Blaži, M., Borović, S., Ivančić-Baće, I., … Müller, N. (2008). Comparative Study on the Antioxidant and Biological Activities of Carvacrol, Thymol, and Eugenol Derivatives. Journal of Agricultural and Food Chemistry, 56(11), 3989–3996. http://doi.org/10.1021/jf073272v<br /> <br /> ===Research pledge===<br /> I, Sarah Lang, have read the Chem/Bioc 430 course syllabus and understand the general structure and expectations of the research program. The above material was prepared after consultation, and in conjunction with my research advisor.</div>

Sarahalang535 http://205.166.159.208/wiki/index.php?title=Sarah_Lang_Chem430_F16&diff=3072 2016-09-07T04:24:56Z

<p>Sarahalang535: /* Research Times */</p> <hr /> <div>Chemistry/Biochemistry Research 430<br /> :Fall 2016<br /> :Sarah Lang<br /> :Senior Biochemistry Major<br /> <br /> ==Research Times==<br /> Mon 2:00-6:00pm<br /> :section 01 = 0.25 credit = 4 hours per week.<br /> <br /> ==Proposed Research Project==<br /> ===The Enzymatic Oxidation of Eugenol and Methyleugenol===<br /> <br /> ===General Information===<br /> :Advisor: Brad Sturgeon<br /> :Other research student collaborators: <br /> :Other Research Collaborators:<br /> <br /> ===Proposal===<br /> The goal of this project is to understand the enzymatic oxidation of biophenols--specifically, of eugenol and methyleugenol. Both eugenol and methyleugenol are commonly found in flavoring components of foods, and recently, connections have been made between methyleugenol (and to a lesser extent, eugenol) and increased incidence of malignant tumors at various tissue sites (''1''). Furthermore, studies have shown that both eugenol and methyleugenol undergo peroxidative metabolism to form free radicals, but little is known about the details of the mechanism behind this oxidation. My focus will be on gathering data on this particular mechanism.<br /> <br /> ===Instruments to be used===<br /> <br /> HPLC<br /> <br /> ===References (2 minimum)===<br /> 1. Sipe, H. J., Lardinois, O. M., &amp; Mason, R. P. (2014). Free Radical Metabolism of Methyleugenol and Related Compounds. Chemical Research in Toxicology, 27(4), 483–489. http://doi.org/10.1021/tx400256b<br /> <br /> 2. Mastelić, J., Jerković, I., Blažević, I., Poljak-Blaži, M., Borović, S., Ivančić-Baće, I., … Müller, N. (2008). Comparative Study on the Antioxidant and Biological Activities of Carvacrol, Thymol, and Eugenol Derivatives. Journal of Agricultural and Food Chemistry, 56(11), 3989–3996. http://doi.org/10.1021/jf073272v<br /> <br /> ===Research pledge===<br /> I, Sarah Lang, have read the Chem/Bioc 430 course syllabus and understand the general structure and expectations of the research program. The above material was prepared after consultation, and in conjunction with my research advisor.</div>

Sarahalang535 http://205.166.159.208/wiki/index.php?title=Sarah_Lang_Chem430_F16&diff=3071 2016-09-07T04:24:41Z

<p>Sarahalang535: /* Research Times */</p> <hr /> <div>Chemistry/Biochemistry Research 430<br /> :Fall 2016<br /> :Sarah Lang<br /> :Senior Biochemistry Major<br /> <br /> ==Research Times==<br /> Mon 2:00-5:00pm<br /> :section 01 = 0.25 credit = 4 hours per week.<br /> <br /> ==Proposed Research Project==<br /> ===The Enzymatic Oxidation of Eugenol and Methyleugenol===<br /> <br /> ===General Information===<br /> :Advisor: Brad Sturgeon<br /> :Other research student collaborators: <br /> :Other Research Collaborators:<br /> <br /> ===Proposal===<br /> The goal of this project is to understand the enzymatic oxidation of biophenols--specifically, of eugenol and methyleugenol. Both eugenol and methyleugenol are commonly found in flavoring components of foods, and recently, connections have been made between methyleugenol (and to a lesser extent, eugenol) and increased incidence of malignant tumors at various tissue sites (''1''). Furthermore, studies have shown that both eugenol and methyleugenol undergo peroxidative metabolism to form free radicals, but little is known about the details of the mechanism behind this oxidation. My focus will be on gathering data on this particular mechanism.<br /> <br /> ===Instruments to be used===<br /> <br /> HPLC<br /> <br /> ===References (2 minimum)===<br /> 1. Sipe, H. J., Lardinois, O. M., &amp; Mason, R. P. (2014). Free Radical Metabolism of Methyleugenol and Related Compounds. Chemical Research in Toxicology, 27(4), 483–489. http://doi.org/10.1021/tx400256b<br /> <br /> 2. Mastelić, J., Jerković, I., Blažević, I., Poljak-Blaži, M., Borović, S., Ivančić-Baće, I., … Müller, N. (2008). Comparative Study on the Antioxidant and Biological Activities of Carvacrol, Thymol, and Eugenol Derivatives. Journal of Agricultural and Food Chemistry, 56(11), 3989–3996. http://doi.org/10.1021/jf073272v<br /> <br /> ===Research pledge===<br /> I, Sarah Lang, have read the Chem/Bioc 430 course syllabus and understand the general structure and expectations of the research program. The above material was prepared after consultation, and in conjunction with my research advisor.</div>

Sarahalang535 http://205.166.159.208/wiki/index.php?title=Sarah_Lang_Chem430_F16&diff=3070 2016-09-07T04:23:19Z

<p>Sarahalang535: /* Research Times */</p> <hr /> <div>Chemistry/Biochemistry Research 430<br /> :Fall 2016<br /> :Sarah Lang<br /> :Senior Biochemistry Major<br /> <br /> ==Research Times==<br /> Mon 2:00-6:00pm<br /> :section 01 = 0.25 credit = 4 hours per week.<br /> <br /> ==Proposed Research Project==<br /> ===The Enzymatic Oxidation of Eugenol and Methyleugenol===<br /> <br /> ===General Information===<br /> :Advisor: Brad Sturgeon<br /> :Other research student collaborators: <br /> :Other Research Collaborators:<br /> <br /> ===Proposal===<br /> The goal of this project is to understand the enzymatic oxidation of biophenols--specifically, of eugenol and methyleugenol. Both eugenol and methyleugenol are commonly found in flavoring components of foods, and recently, connections have been made between methyleugenol (and to a lesser extent, eugenol) and increased incidence of malignant tumors at various tissue sites (''1''). Furthermore, studies have shown that both eugenol and methyleugenol undergo peroxidative metabolism to form free radicals, but little is known about the details of the mechanism behind this oxidation. My focus will be on gathering data on this particular mechanism.<br /> <br /> ===Instruments to be used===<br /> <br /> HPLC<br /> <br /> ===References (2 minimum)===<br /> 1. Sipe, H. J., Lardinois, O. M., &amp; Mason, R. P. (2014). Free Radical Metabolism of Methyleugenol and Related Compounds. Chemical Research in Toxicology, 27(4), 483–489. http://doi.org/10.1021/tx400256b<br /> <br /> 2. Mastelić, J., Jerković, I., Blažević, I., Poljak-Blaži, M., Borović, S., Ivančić-Baće, I., … Müller, N. (2008). Comparative Study on the Antioxidant and Biological Activities of Carvacrol, Thymol, and Eugenol Derivatives. Journal of Agricultural and Food Chemistry, 56(11), 3989–3996. http://doi.org/10.1021/jf073272v<br /> <br /> ===Research pledge===<br /> I, Sarah Lang, have read the Chem/Bioc 430 course syllabus and understand the general structure and expectations of the research program. The above material was prepared after consultation, and in conjunction with my research advisor.</div>

Sarahalang535 http://205.166.159.208/wiki/index.php?title=Sarah_Lang_Chem430_F16&diff=3069 2016-09-07T04:21:42Z

<p>Sarahalang535: </p> <hr /> <div>Chemistry/Biochemistry Research 430<br /> :Fall 2016<br /> :Sarah Lang<br /> :Senior Biochemistry Major<br /> <br /> ==Research Times==<br /> M 2:00-6:00pm<br /> : section 01 = 0.25 credit = 4 hours per week.<br /> <br /> ==Proposed Research Project==<br /> ===The Enzymatic Oxidation of Eugenol and Methyleugenol===<br /> <br /> ===General Information===<br /> :Advisor: Brad Sturgeon<br /> :Other research student collaborators: <br /> :Other Research Collaborators:<br /> <br /> ===Proposal===<br /> The goal of this project is to understand the enzymatic oxidation of biophenols--specifically, of eugenol and methyleugenol. Both eugenol and methyleugenol are commonly found in flavoring components of foods, and recently, connections have been made between methyleugenol (and to a lesser extent, eugenol) and increased incidence of malignant tumors at various tissue sites (''1''). Furthermore, studies have shown that both eugenol and methyleugenol undergo peroxidative metabolism to form free radicals, but little is known about the details of the mechanism behind this oxidation. My focus will be on gathering data on this particular mechanism.<br /> <br /> ===Instruments to be used===<br /> <br /> HPLC<br /> <br /> ===References (2 minimum)===<br /> 1. Sipe, H. J., Lardinois, O. M., &amp; Mason, R. P. (2014). Free Radical Metabolism of Methyleugenol and Related Compounds. Chemical Research in Toxicology, 27(4), 483–489. http://doi.org/10.1021/tx400256b<br /> <br /> 2. Mastelić, J., Jerković, I., Blažević, I., Poljak-Blaži, M., Borović, S., Ivančić-Baće, I., … Müller, N. (2008). Comparative Study on the Antioxidant and Biological Activities of Carvacrol, Thymol, and Eugenol Derivatives. Journal of Agricultural and Food Chemistry, 56(11), 3989–3996. http://doi.org/10.1021/jf073272v<br /> <br /> ===Research pledge===<br /> I, Sarah Lang, have read the Chem/Bioc 430 course syllabus and understand the general structure and expectations of the research program. The above material was prepared after consultation, and in conjunction with my research advisor.</div>

Sarahalang535 http://205.166.159.208/wiki/index.php?title=Sarah_Lang_Chem430_F16&diff=3068 2016-09-07T02:31:55Z

<p>Sarahalang535: /* Instruments to be used */</p> <hr /> <div>Chemistry/Biochemistry Research 430<br /> :Fall 2016<br /> :Sarah Lang<br /> :Senior Biochemistry Major<br /> <br /> ==Research Times==<br /> M 3:00-5:00 pm;<br /> F 9:00-11:00 am<br /> : section 01 = 0.25 credit = 4 hours per week.<br /> <br /> ==Proposed Research Project==<br /> ===The Enzymatic Oxidation of Eugenol and Methyleugenol===<br /> <br /> ===General Information===<br /> :Advisor: Brad Sturgeon<br /> :Other research student collaborators: <br /> :Other Research Collaborators:<br /> <br /> ===Proposal===<br /> The goal of this project is to understand the enzymatic oxidation of biophenols--specifically, of eugenol and methyleugenol. Both eugenol and methyleugenol are commonly found in flavoring components of foods, and recently, connections have been made between methyleugenol (and to a lesser extent, eugenol) and increased incidence of malignant tumors at various tissue sites (''1''). Furthermore, studies have shown that both eugenol and methyleugenol undergo peroxidative metabolism to form free radicals, but little is known about the details of the mechanism behind this oxidation. My focus will be on gathering data on this particular mechanism.<br /> <br /> ===Instruments to be used===<br /> <br /> HPLC<br /> <br /> ===References (2 minimum)===<br /> 1. Sipe, H. J., Lardinois, O. M., &amp; Mason, R. P. (2014). Free Radical Metabolism of Methyleugenol and Related Compounds. Chemical Research in Toxicology, 27(4), 483–489. http://doi.org/10.1021/tx400256b<br /> <br /> 2. Mastelić, J., Jerković, I., Blažević, I., Poljak-Blaži, M., Borović, S., Ivančić-Baće, I., … Müller, N. (2008). Comparative Study on the Antioxidant and Biological Activities of Carvacrol, Thymol, and Eugenol Derivatives. Journal of Agricultural and Food Chemistry, 56(11), 3989–3996. http://doi.org/10.1021/jf073272v<br /> <br /> ===Research pledge===<br /> I, Sarah Lang, have read the Chem/Bioc 430 course syllabus and understand the general structure and expectations of the research program. The above material was prepared after consultation, and in conjunction with my research advisor.</div>

Sarahalang535 http://205.166.159.208/wiki/index.php?title=Sarah_Lang_Chem430_F16&diff=3067 2016-09-07T02:30:06Z

<p>Sarahalang535: /* Proposal */</p> <hr /> <div>Chemistry/Biochemistry Research 430<br /> :Fall 2016<br /> :Sarah Lang<br /> :Senior Biochemistry Major<br /> <br /> ==Research Times==<br /> M 3:00-5:00 pm;<br /> F 9:00-11:00 am<br /> : section 01 = 0.25 credit = 4 hours per week.<br /> <br /> ==Proposed Research Project==<br /> ===The Enzymatic Oxidation of Eugenol and Methyleugenol===<br /> <br /> ===General Information===<br /> :Advisor: Brad Sturgeon<br /> :Other research student collaborators: <br /> :Other Research Collaborators:<br /> <br /> ===Proposal===<br /> The goal of this project is to understand the enzymatic oxidation of biophenols--specifically, of eugenol and methyleugenol. Both eugenol and methyleugenol are commonly found in flavoring components of foods, and recently, connections have been made between methyleugenol (and to a lesser extent, eugenol) and increased incidence of malignant tumors at various tissue sites (''1''). Furthermore, studies have shown that both eugenol and methyleugenol undergo peroxidative metabolism to form free radicals, but little is known about the details of the mechanism behind this oxidation. My focus will be on gathering data on this particular mechanism.<br /> <br /> ===Instruments to be used===<br /> <br /> ===References (2 minimum)===<br /> 1. Sipe, H. J., Lardinois, O. M., &amp; Mason, R. P. (2014). Free Radical Metabolism of Methyleugenol and Related Compounds. Chemical Research in Toxicology, 27(4), 483–489. http://doi.org/10.1021/tx400256b<br /> <br /> 2. Mastelić, J., Jerković, I., Blažević, I., Poljak-Blaži, M., Borović, S., Ivančić-Baće, I., … Müller, N. (2008). Comparative Study on the Antioxidant and Biological Activities of Carvacrol, Thymol, and Eugenol Derivatives. Journal of Agricultural and Food Chemistry, 56(11), 3989–3996. http://doi.org/10.1021/jf073272v<br /> <br /> ===Research pledge===<br /> I, Sarah Lang, have read the Chem/Bioc 430 course syllabus and understand the general structure and expectations of the research program. The above material was prepared after consultation, and in conjunction with my research advisor.</div>

Sarahalang535 http://205.166.159.208/wiki/index.php?title=Sarah_Lang_Chem430_F16&diff=3066 2016-09-07T02:25:33Z

<p>Sarahalang535: /* References (2 minimum) */</p> <hr /> <div>Chemistry/Biochemistry Research 430<br /> :Fall 2016<br /> :Sarah Lang<br /> :Senior Biochemistry Major<br /> <br /> ==Research Times==<br /> M 3:00-5:00 pm;<br /> F 9:00-11:00 am<br /> : section 01 = 0.25 credit = 4 hours per week.<br /> <br /> ==Proposed Research Project==<br /> ===The Enzymatic Oxidation of Eugenol and Methyleugenol===<br /> <br /> ===General Information===<br /> :Advisor: Brad Sturgeon<br /> :Other research student collaborators: <br /> :Other Research Collaborators:<br /> <br /> ===Proposal===<br /> The goal of this project is to understand the enzymatic oxidation of biophenols--specifically, of eugenol and methyleugenol. Both eugenol and methyleugenol are commonly found in flavoring components of foods, and recently, connections have been made between methyleugenol (and to a lesser extent, eugenol) and increased incidence of malignant tumors at various tissue sites. Furthermore, studies have shown that both eugenol and methyleugenol undergo peroxidative metabolism to form free radicals, but little is known about the details of the mechanism behind this oxidation. My focus will be on gathering data on this particular mechanism.<br /> <br /> ===Instruments to be used===<br /> <br /> ===References (2 minimum)===<br /> 1. Sipe, H. J., Lardinois, O. M., &amp; Mason, R. P. (2014). Free Radical Metabolism of Methyleugenol and Related Compounds. Chemical Research in Toxicology, 27(4), 483–489. http://doi.org/10.1021/tx400256b<br /> <br /> 2. Mastelić, J., Jerković, I., Blažević, I., Poljak-Blaži, M., Borović, S., Ivančić-Baće, I., … Müller, N. (2008). Comparative Study on the Antioxidant and Biological Activities of Carvacrol, Thymol, and Eugenol Derivatives. Journal of Agricultural and Food Chemistry, 56(11), 3989–3996. http://doi.org/10.1021/jf073272v<br /> <br /> ===Research pledge===<br /> I, Sarah Lang, have read the Chem/Bioc 430 course syllabus and understand the general structure and expectations of the research program. The above material was prepared after consultation, and in conjunction with my research advisor.</div>

Sarahalang535 http://205.166.159.208/wiki/index.php?title=Sarah_Lang_Chem430_F16&diff=3065 2016-09-07T02:25:21Z

<p>Sarahalang535: /* References (2 minimum) */</p> <hr /> <div>Chemistry/Biochemistry Research 430<br /> :Fall 2016<br /> :Sarah Lang<br /> :Senior Biochemistry Major<br /> <br /> ==Research Times==<br /> M 3:00-5:00 pm;<br /> F 9:00-11:00 am<br /> : section 01 = 0.25 credit = 4 hours per week.<br /> <br /> ==Proposed Research Project==<br /> ===The Enzymatic Oxidation of Eugenol and Methyleugenol===<br /> <br /> ===General Information===<br /> :Advisor: Brad Sturgeon<br /> :Other research student collaborators: <br /> :Other Research Collaborators:<br /> <br /> ===Proposal===<br /> The goal of this project is to understand the enzymatic oxidation of biophenols--specifically, of eugenol and methyleugenol. Both eugenol and methyleugenol are commonly found in flavoring components of foods, and recently, connections have been made between methyleugenol (and to a lesser extent, eugenol) and increased incidence of malignant tumors at various tissue sites. Furthermore, studies have shown that both eugenol and methyleugenol undergo peroxidative metabolism to form free radicals, but little is known about the details of the mechanism behind this oxidation. My focus will be on gathering data on this particular mechanism.<br /> <br /> ===Instruments to be used===<br /> <br /> ===References (2 minimum)===<br /> 1. Sipe, H. J., Lardinois, O. M., &amp; Mason, R. P. (2014). Free Radical Metabolism of Methyleugenol and Related Compounds. Chemical Research in Toxicology, 27(4), 483–489. http://doi.org/10.1021/tx400256b<br /> 2. Mastelić, J., Jerković, I., Blažević, I., Poljak-Blaži, M., Borović, S., Ivančić-Baće, I., … Müller, N. (2008). Comparative Study on the Antioxidant and Biological Activities of Carvacrol, Thymol, and Eugenol Derivatives. Journal of Agricultural and Food Chemistry, 56(11), 3989–3996. http://doi.org/10.1021/jf073272v<br /> <br /> ===Research pledge===<br /> I, Sarah Lang, have read the Chem/Bioc 430 course syllabus and understand the general structure and expectations of the research program. The above material was prepared after consultation, and in conjunction with my research advisor.</div>

Sarahalang535 http://205.166.159.208/wiki/index.php?title=Sarah_Lang_Chem430_F16&diff=3064 2016-09-07T02:24:22Z

<p>Sarahalang535: /* Proposal */</p> <hr /> <div>Chemistry/Biochemistry Research 430<br /> :Fall 2016<br /> :Sarah Lang<br /> :Senior Biochemistry Major<br /> <br /> ==Research Times==<br /> M 3:00-5:00 pm;<br /> F 9:00-11:00 am<br /> : section 01 = 0.25 credit = 4 hours per week.<br /> <br /> ==Proposed Research Project==<br /> ===The Enzymatic Oxidation of Eugenol and Methyleugenol===<br /> <br /> ===General Information===<br /> :Advisor: Brad Sturgeon<br /> :Other research student collaborators: <br /> :Other Research Collaborators:<br /> <br /> ===Proposal===<br /> The goal of this project is to understand the enzymatic oxidation of biophenols--specifically, of eugenol and methyleugenol. Both eugenol and methyleugenol are commonly found in flavoring components of foods, and recently, connections have been made between methyleugenol (and to a lesser extent, eugenol) and increased incidence of malignant tumors at various tissue sites. Furthermore, studies have shown that both eugenol and methyleugenol undergo peroxidative metabolism to form free radicals, but little is known about the details of the mechanism behind this oxidation. My focus will be on gathering data on this particular mechanism.<br /> <br /> ===Instruments to be used===<br /> <br /> ===References (2 minimum)===<br /> *Sipe, H. J., Lardinois, O. M., &amp; Mason, R. P. (2014). Free Radical Metabolism of Methyleugenol and Related Compounds. Chemical Research in Toxicology, 27(4), 483–489. http://doi.org/10.1021/tx400256b<br /> *Mastelić, J., Jerković, I., Blažević, I., Poljak-Blaži, M., Borović, S., Ivančić-Baće, I., … Müller, N. (2008). Comparative Study on the Antioxidant and Biological Activities of Carvacrol, Thymol, and Eugenol Derivatives. Journal of Agricultural and Food Chemistry, 56(11), 3989–3996. http://doi.org/10.1021/jf073272v<br /> <br /> ===Research pledge===<br /> I, Sarah Lang, have read the Chem/Bioc 430 course syllabus and understand the general structure and expectations of the research program. The above material was prepared after consultation, and in conjunction with my research advisor.</div>

Sarahalang535 http://205.166.159.208/wiki/index.php?title=Sarah_Lang_Chem430_F16&diff=3063 2016-09-07T01:55:30Z

<p>Sarahalang535: /* References (2 minimum) */</p> <hr /> <div>Chemistry/Biochemistry Research 430<br /> :Fall 2016<br /> :Sarah Lang<br /> :Senior Biochemistry Major<br /> <br /> ==Research Times==<br /> M 3:00-5:00 pm;<br /> F 9:00-11:00 am<br /> : section 01 = 0.25 credit = 4 hours per week.<br /> <br /> ==Proposed Research Project==<br /> ===The Enzymatic Oxidation of Eugenol and Methyleugenol===<br /> <br /> ===General Information===<br /> :Advisor: Brad Sturgeon<br /> :Other research student collaborators: <br /> :Other Research Collaborators:<br /> <br /> ===Proposal===<br /> <br /> ===Instruments to be used===<br /> <br /> ===References (2 minimum)===<br /> *Sipe, H. J., Lardinois, O. M., &amp; Mason, R. P. (2014). Free Radical Metabolism of Methyleugenol and Related Compounds. Chemical Research in Toxicology, 27(4), 483–489. http://doi.org/10.1021/tx400256b<br /> *Mastelić, J., Jerković, I., Blažević, I., Poljak-Blaži, M., Borović, S., Ivančić-Baće, I., … Müller, N. (2008). Comparative Study on the Antioxidant and Biological Activities of Carvacrol, Thymol, and Eugenol Derivatives. Journal of Agricultural and Food Chemistry, 56(11), 3989–3996. http://doi.org/10.1021/jf073272v<br /> <br /> ===Research pledge===<br /> I, Sarah Lang, have read the Chem/Bioc 430 course syllabus and understand the general structure and expectations of the research program. The above material was prepared after consultation, and in conjunction with my research advisor.</div>

Sarahalang535 http://205.166.159.208/wiki/index.php?title=Sarah_Lang_Chem430_F16&diff=3062 2016-09-07T01:52:58Z

<p>Sarahalang535: /* References (2 minimum) */</p> <hr /> <div>Chemistry/Biochemistry Research 430<br /> :Fall 2016<br /> :Sarah Lang<br /> :Senior Biochemistry Major<br /> <br /> ==Research Times==<br /> M 3:00-5:00 pm;<br /> F 9:00-11:00 am<br /> : section 01 = 0.25 credit = 4 hours per week.<br /> <br /> ==Proposed Research Project==<br /> ===The Enzymatic Oxidation of Eugenol and Methyleugenol===<br /> <br /> ===General Information===<br /> :Advisor: Brad Sturgeon<br /> :Other research student collaborators: <br /> :Other Research Collaborators:<br /> <br /> ===Proposal===<br /> <br /> ===Instruments to be used===<br /> <br /> ===References (2 minimum)===<br /> *Sipe, H. J., Lardinois, O. M., &amp; Mason, R. P. (2014). Free Radical Metabolism of Methyleugenol and Related Compounds. Chemical Research in Toxicology, 27(4), 483–489. http://doi.org/10.1021/tx400256b<br /> <br /> ===Research pledge===<br /> I, Sarah Lang, have read the Chem/Bioc 430 course syllabus and understand the general structure and expectations of the research program. The above material was prepared after consultation, and in conjunction with my research advisor.</div>

Sarahalang535 http://205.166.159.208/wiki/index.php?title=Sarah_Lang_Chem430_F16&diff=3061 2016-09-07T01:45:03Z

<p>Sarahalang535: /* Research Times */</p> <hr /> <div>Chemistry/Biochemistry Research 430<br /> :Fall 2016<br /> :Sarah Lang<br /> :Senior Biochemistry Major<br /> <br /> ==Research Times==<br /> M 3:00-5:00 pm;<br /> F 9:00-11:00 am<br /> : section 01 = 0.25 credit = 4 hours per week.<br /> <br /> ==Proposed Research Project==<br /> ===The Enzymatic Oxidation of Eugenol and Methyleugenol===<br /> <br /> ===General Information===<br /> :Advisor: Brad Sturgeon<br /> :Other research student collaborators: <br /> :Other Research Collaborators:<br /> <br /> ===Proposal===<br /> <br /> ===Instruments to be used===<br /> <br /> ===References (2 minimum)===<br /> <br /> ===Research pledge===<br /> I, Sarah Lang, have read the Chem/Bioc 430 course syllabus and understand the general structure and expectations of the research program. The above material was prepared after consultation, and in conjunction with my research advisor.</div>

Sarahalang535 http://205.166.159.208/wiki/index.php?title=Sarah_Lang_Chem430_F16&diff=3060 2016-09-07T01:43:46Z

<p>Sarahalang535: /* Enter Project Title here */</p> <hr /> <div>Chemistry/Biochemistry Research 430<br /> :Fall 2016<br /> :Sarah Lang<br /> :Senior Biochemistry Major<br /> <br /> ==Research Times==<br /> M 3:00-5:00 pm;<br /> W 9:00-11:00 am<br /> : section 01 = 0.25 credit = 4 hours per week.<br /> <br /> ==Proposed Research Project==<br /> ===The Enzymatic Oxidation of Eugenol and Methyleugenol===<br /> <br /> ===General Information===<br /> :Advisor: Brad Sturgeon<br /> :Other research student collaborators: <br /> :Other Research Collaborators:<br /> <br /> ===Proposal===<br /> <br /> ===Instruments to be used===<br /> <br /> ===References (2 minimum)===<br /> <br /> ===Research pledge===<br /> I, Sarah Lang, have read the Chem/Bioc 430 course syllabus and understand the general structure and expectations of the research program. The above material was prepared after consultation, and in conjunction with my research advisor.</div>

Sarahalang535 http://205.166.159.208/wiki/index.php?title=Sarah_Lang_Chem430_F16&diff=3059 2016-09-07T01:40:10Z

<p>Sarahalang535: /* Research Times */</p> <hr /> <div>Chemistry/Biochemistry Research 430<br /> :Fall 2016<br /> :Sarah Lang<br /> :Senior Biochemistry Major<br /> <br /> ==Research Times==<br /> M 3:00-5:00 pm;<br /> W 9:00-11:00 am<br /> : section 01 = 0.25 credit = 4 hours per week.<br /> <br /> ==Proposed Research Project==<br /> ===Enter Project Title here===<br /> <br /> ===General Information===<br /> :Advisor: Brad Sturgeon<br /> :Other research student collaborators: <br /> :Other Research Collaborators:<br /> <br /> ===Proposal===<br /> <br /> ===Instruments to be used===<br /> <br /> ===References (2 minimum)===<br /> <br /> ===Research pledge===<br /> I, Sarah Lang, have read the Chem/Bioc 430 course syllabus and understand the general structure and expectations of the research program. The above material was prepared after consultation, and in conjunction with my research advisor.</div>

Sarahalang535 http://205.166.159.208/wiki/index.php?title=Sarah_Lang_Chem430_F16&diff=3058 2016-09-07T01:39:30Z

<p>Sarahalang535: Created page with &quot;Chemistry/Biochemistry Research 430 :Fall 2016 :Sarah Lang :Senior Biochemistry Major ==Research Times== M 3:00-5:00 pm W 9:00-11:00 am : section 01 = 0.25 credit = 4 hours p...&quot;</p> <hr /> <div>Chemistry/Biochemistry Research 430<br /> :Fall 2016<br /> :Sarah Lang<br /> :Senior Biochemistry Major<br /> <br /> ==Research Times==<br /> M 3:00-5:00 pm<br /> W 9:00-11:00 am<br /> : section 01 = 0.25 credit = 4 hours per week.<br /> <br /> ==Proposed Research Project==<br /> ===Enter Project Title here===<br /> <br /> ===General Information===<br /> :Advisor: Brad Sturgeon<br /> :Other research student collaborators: <br /> :Other Research Collaborators:<br /> <br /> ===Proposal===<br /> <br /> ===Instruments to be used===<br /> <br /> ===References (2 minimum)===<br /> <br /> ===Research pledge===<br /> I, Sarah Lang, have read the Chem/Bioc 430 course syllabus and understand the general structure and expectations of the research program. The above material was prepared after consultation, and in conjunction with my research advisor.</div>

Sarahalang535 http://205.166.159.208/wiki/index.php?title=Lang_Research_2016&diff=3057 2016-09-07T01:26:35Z

<p>Sarahalang535: </p> <hr /> <div>==Proposed Project Description==<br /> <br /> The goal of this project is to understand the enzymatic oxidation of biophenols--specifically, of eugenol and methyleugenol. In order to collect data on these oxidations, we will use HPLC (High Performance Liquid Chromatography). My focus will also be on comparing new data that I have collected to previous data by [http://esr.monmsci.net/wiki/index.php/Pauzi_Research_Spring_2016 Matt (Amad Pauzi)], who initially worked on this project.<br /> <br /> ==Abstract==<br /> <br /> (To be included)</div>

Sarahalang535 http://205.166.159.208/wiki/index.php?title=Lang_Research_2016&diff=3004 2016-09-05T21:02:02Z

<p>Sarahalang535: /* Proposed Project Description */</p> <hr /> <div>==Proposed Project Description==<br /> <br /> The goal of this project is to understand the enzymatic oxidation of biophenols--specifically, of eugenol and methyleugenol. In order to collect data on these oxidations, we will use HPLC (High Performance Liquid Chromatography). My focus will also be on comparing new data that I have collected to previous data by [http://esr.monmsci.net/wiki/index.php/Pauzi_Research_Spring_2016 Matt (Amad Pauzi)], who initially worked on this project.<br /> <br /> ==Abstract==</div>

Sarahalang535 http://205.166.159.208/wiki/index.php?title=Lang_Research_2016&diff=3003 2016-09-05T21:00:00Z

<p>Sarahalang535: /* Proposed Project Description */</p> <hr /> <div>==Proposed Project Description==<br /> <br /> The goal of this project is to understand the enzymatic oxidation of biophenols--specifically, eugenol and methyleugenol. In order to do so, we will use HPLC (High Performance Liquid Chromatography) in order to collect data. My focus will also be on comparing new data that I have collected to previous data by [http://esr.monmsci.net/wiki/index.php/Pauzi_Research_Spring_2016 Matt (Amad Pauzi)].<br /> <br /> ==Abstract==</div>

Sarahalang535 http://205.166.159.208/wiki/index.php?title=Lang_Research_2016&diff=3002 2016-09-05T20:57:52Z

<p>Sarahalang535: /* Proposed Project Description */</p> <hr /> <div>==Proposed Project Description==<br /> <br /> The goal of this project is to understand the enzymatic oxidation of biophenols--specifically, eugenol and methyleugenol. In order to do so, we will use HPLC (High Performance Liquid Chromatography) in order to collect data. My focus will also be on comparing new data that I have collected to previous collections made by<br /> <br /> ==Abstract==</div>

Sarahalang535 http://205.166.159.208/wiki/index.php?title=Lang_Research_2016&diff=3001 2016-09-05T20:55:08Z

<p>Sarahalang535: /* Proposed Project Description */</p> <hr /> <div>==Proposed Project Description==<br /> <br /> The goal of this project is to understand the enzymatic oxidation of biophenols--specifically, eugenol and methyleugenol. In order to do so, we will use HPLC (High Performance Liquid Chromatography).<br /> <br /> ==Abstract==</div>

Sarahalang535 http://205.166.159.208/wiki/index.php?title=Lang_Research_2016&diff=3000 2016-09-05T20:51:18Z

<p>Sarahalang535: Created page with &quot;==Proposed Project Description== The goal of this project is to ==Abstract==&quot;</p> <hr /> <div>==Proposed Project Description==<br /> <br /> The goal of this project is to <br /> <br /> ==Abstract==</div>

Sarahalang535 http://205.166.159.208/wiki/index.php?title=Biophenols_as_Secondary_Plant_Metabolites&diff=2999 2016-09-05T20:48:02Z

<p>Sarahalang535: /* Eugenol/Methyl Eugenol (from Clove) */</p> <hr /> <div>==[https://en.wikipedia.org/wiki/Lignin Lignin] monomers==<br /> [[File:Ligin Monomer radicals.png|200px|thumb|center|ESR data from HRP oxidation of lignin monomers]]<br /> :[https://en.wikipedia.org/wiki/Paracoumaryl_alcohol p-coumaryl alcohol]<br /> :[https://en.wikipedia.org/wiki/Coniferyl_alcohol coniferyl alcohol]<br /> :[https://en.wikipedia.org/wiki/Sinapyl_alcohol sinapyl alcohol]<br /> <br /> ==Lignans (General)==<br /> <br /> ==Eugenol/Methyl Eugenol (from Clove)==<br /> :Previous work by [http://esr.monmsci.net/wiki/index.php/Pauzi_Research_Spring_2016 Matt (Amad Pauzi)]<br /> :Current (Fall 2016) work by [http://esr.monmsci.net/wiki/index.php/Lang_Research_2016 Sarah Lang].<br /> <br /> ==Curcumin (from Turmeric)==<br /> :Project started (Fall 2016) by Stephanie Saey.<br /> :[[Media:Turmeric ref 01.pdf|Reference]]; purification from plant material.<br /> <br /> ==Chlorogenic Acid (from green coffee beans)==<br /> :Project started by [[Chris_Knutson]].<br /> :Current project on isolation from green coffee beans by Alan Kuhlemier.</div>

Sarahalang535 http://205.166.159.208/wiki/index.php?title=Biophenols_as_Secondary_Plant_Metabolites&diff=2998 2016-09-05T20:47:19Z

<p>Sarahalang535: /* Eugenol/Methyl Eugenol (from Clove) */</p> <hr /> <div>==[https://en.wikipedia.org/wiki/Lignin Lignin] monomers==<br /> [[File:Ligin Monomer radicals.png|200px|thumb|center|ESR data from HRP oxidation of lignin monomers]]<br /> :[https://en.wikipedia.org/wiki/Paracoumaryl_alcohol p-coumaryl alcohol]<br /> :[https://en.wikipedia.org/wiki/Coniferyl_alcohol coniferyl alcohol]<br /> :[https://en.wikipedia.org/wiki/Sinapyl_alcohol sinapyl alcohol]<br /> <br /> ==Lignans (General)==<br /> <br /> ==Eugenol/Methyl Eugenol (from Clove)==<br /> :previous work by [http://esr.monmsci.net/wiki/index.php/Pauzi_Research_Spring_2016 Matt (Amad Pauzi)]<br /> :current (Fall 2016) [http://esr.monmsci.net/wiki/index.php/Lang_Research_2016 Sarah Lang].<br /> <br /> ==Curcumin (from Turmeric)==<br /> :Project started (Fall 2016) by Stephanie Saey.<br /> :[[Media:Turmeric ref 01.pdf|Reference]]; purification from plant material.<br /> <br /> ==Chlorogenic Acid (from green coffee beans)==<br /> :Project started by [[Chris_Knutson]].<br /> :Current project on isolation from green coffee beans by Alan Kuhlemier.</div>

Sarahalang535 http://205.166.159.208/wiki/index.php?title=Sarah_Lang&diff=2997 2016-09-05T20:38:06Z

<p>Sarahalang535: /* Other Involvement */</p> <hr /> <div>&lt;!-- Greetings...this text does not show up on the page since it is &quot;hidden&quot; with the surrounding tags --&gt;<br /> &lt;!-- This is the suggested format for current students to organize their information; this is NOT a requirement --&gt;<br /> &lt;!-- To add this structure to your personal wiki page, copy and paste the code below and then remove the &quot;hidden&quot; tags --&gt;<br /> &lt;!-- ...then of course...add your information. Use Mr. Bitchly's text for coding examples...pls add examples to this profile --&gt;<br /> <br /> &lt;!-- ==Personal Information== --&gt;<br /> &lt;!-- ==Undergraduate Research Activities== --&gt;<br /> &lt;!-- ==Presentations== --&gt;<br /> &lt;!-- ==Interests== --&gt;<br /> &lt;!-- ==Career Goals== --&gt;<br /> &lt;!-- --&gt;<br /> &lt;!-- --&gt;<br /> <br /> ==Personal Information==<br /> Senior biochemistry major<br /> <br /> Hometown: Peoria, IL<br /> <br /> Contact Info: 309-265-1518; slang@monmouthcollege.edu<br /> <br /> ==Undergraduate Research Activities==<br /> <br /> Aug 2013: SOfIA (2013) The Effects of Sulfadimethoxine and Malachite Green on the Gregarine Parasite Count of Madagascar Hissing Cockroaches<br /> *Studied how different dosages of two drugs, sulfadimethoxine and malachite green, affected the level of gregarine parasite infection in Madagascar Hissing Cockroaches<br /> *Constructed presentation and poster to present findings at the end of the 3-week research period<br /> <br /> Aug 2015: SOfIA (2015) The Effects of Gregarine Parasites on the Physiology of Madagascar Hissing Cockroaches<br /> *Guided freshman SOfIA students on project creation and served as a mentor throughout the project<br /> *Aided in new students' development of lab, research, and presentation skills<br /> <br /> Current: BIOC 430 with Sturgeon<br /> <br /> ==Presentations==<br /> Aug 2013: (SOfIA poster presentation)''The Effects of Sulfadimethoxine and Malachite Green on the Gregarine Parasite Count of Madagascar Hissing Cockroaches''<br /> <br /> Aug 2015: (SOfIA poster presentation)''The Effects of Gregarine Parasites on the Physiology of Madagascar Hissing Cockroaches''<br /> <br /> ==Other Involvement==<br /> <br /> '''Midwest Journal of Undergraduate Research'''<br /> *Publications Editor: Responsible for formatting the final printed journal using InDesign software<br /> *Developed skills in reviewing and editing undergraduate research papers from a variety of disciplines<br /> <br /> '''Teaching Assistant'''<br /> *Managed lab safety and aided students in proper use of instrumentation<br /> *Provided feedback to students on lab calculations and lab technique<br /> <br /> '''Biology 150 SI'''<br /> *Held sessions outside of regular class time to help students understand a variety of topics<br /> *Organized evening review sessions for exams<br /> *Worked with the Biology 150 professor to construct and distribute study guides for each in-class exam<br /> <br /> '''OSF Summer Student Volunteer'''<br /> *30+ hours spent working in the Adult Medical/Surgical Unit<br /> *Aided in replacing shipments of medical supplies, maintaining sanitization, and ensuring patient comfort whenever possible<br /> *Developed skills in bedside manner, recognition of necessary equipment, and hospital navigation.<br /> <br /> '''Monmouth College Swim Team (2013-2015)'''<br /> *Academic All-Conference Athlete (2013-2015)<br /> *Developed skills in both leading and cooperating on a diverse team of athletes<br /> <br /> ==Career Plans==<br /> <br /> Nurse Practitioner (through direct-entry MSN for non-nursing majors)</div>

Sarahalang535 http://205.166.159.208/wiki/index.php?title=Sarah_Lang&diff=2996 2016-09-05T20:36:45Z

<p>Sarahalang535: /* Presentations */</p> <hr /> <div>&lt;!-- Greetings...this text does not show up on the page since it is &quot;hidden&quot; with the surrounding tags --&gt;<br /> &lt;!-- This is the suggested format for current students to organize their information; this is NOT a requirement --&gt;<br /> &lt;!-- To add this structure to your personal wiki page, copy and paste the code below and then remove the &quot;hidden&quot; tags --&gt;<br /> &lt;!-- ...then of course...add your information. Use Mr. Bitchly's text for coding examples...pls add examples to this profile --&gt;<br /> <br /> &lt;!-- ==Personal Information== --&gt;<br /> &lt;!-- ==Undergraduate Research Activities== --&gt;<br /> &lt;!-- ==Presentations== --&gt;<br /> &lt;!-- ==Interests== --&gt;<br /> &lt;!-- ==Career Goals== --&gt;<br /> &lt;!-- --&gt;<br /> &lt;!-- --&gt;<br /> <br /> ==Personal Information==<br /> Senior biochemistry major<br /> <br /> Hometown: Peoria, IL<br /> <br /> Contact Info: 309-265-1518; slang@monmouthcollege.edu<br /> <br /> ==Undergraduate Research Activities==<br /> <br /> Aug 2013: SOfIA (2013) The Effects of Sulfadimethoxine and Malachite Green on the Gregarine Parasite Count of Madagascar Hissing Cockroaches<br /> *Studied how different dosages of two drugs, sulfadimethoxine and malachite green, affected the level of gregarine parasite infection in Madagascar Hissing Cockroaches<br /> *Constructed presentation and poster to present findings at the end of the 3-week research period<br /> <br /> Aug 2015: SOfIA (2015) The Effects of Gregarine Parasites on the Physiology of Madagascar Hissing Cockroaches<br /> *Guided freshman SOfIA students on project creation and served as a mentor throughout the project<br /> *Aided in new students' development of lab, research, and presentation skills<br /> <br /> Current: BIOC 430 with Sturgeon<br /> <br /> ==Presentations==<br /> Aug 2013: (SOfIA poster presentation)''The Effects of Sulfadimethoxine and Malachite Green on the Gregarine Parasite Count of Madagascar Hissing Cockroaches''<br /> <br /> Aug 2015: (SOfIA poster presentation)''The Effects of Gregarine Parasites on the Physiology of Madagascar Hissing Cockroaches''<br /> <br /> ==Other Involvement==<br /> <br /> '''Midwest Journal of Undergraduate Research'''<br /> *Publications Editor: Responsible for formatting the final printed journal using InDesign software<br /> *Developed skills in reviewing and editing undergraduate research papers from a variety of disciplines<br /> <br /> '''Teaching Assistant'''<br /> *Managed lab safety and aided students in proper use of instrumentation<br /> *Provided feedback to students on lab calculations and lab technique<br /> <br /> '''Biology 150 SI'''<br /> *Held sessions outside of regular class time to help students understand a variety of topics<br /> *Organized evening review sessions for exams<br /> *Worked with the Biology 150 professor to construct and distribute study guides for each in-class exam<br /> <br /> '''OSF Summer Student Volunteer'''<br /> *30+ hours spent working in the Adult Medical/Surgical Unit<br /> *Aided in replacing shipments of medical supplies, sanitizing stations on the floor, and ensuring patient comfort whenever possible<br /> *Developed skills in bedside manner, recognition of necessary equipment, and hospital navigation.<br /> <br /> '''Monmouth College Swim Team (2013-2015)'''<br /> *Academic All-Conference Athlete (2013-2015)<br /> *Developed skills in both leading and cooperating on a diverse team of athletes<br /> <br /> ==Career Plans==<br /> <br /> Nurse Practitioner (through direct-entry MSN for non-nursing majors)</div>

Sarahalang535 http://205.166.159.208/wiki/index.php?title=Sarah_Lang&diff=2995 2016-09-05T20:36:17Z

<p>Sarahalang535: /* Other Involvement */</p> <hr /> <div>&lt;!-- Greetings...this text does not show up on the page since it is &quot;hidden&quot; with the surrounding tags --&gt;<br /> &lt;!-- This is the suggested format for current students to organize their information; this is NOT a requirement --&gt;<br /> &lt;!-- To add this structure to your personal wiki page, copy and paste the code below and then remove the &quot;hidden&quot; tags --&gt;<br /> &lt;!-- ...then of course...add your information. Use Mr. Bitchly's text for coding examples...pls add examples to this profile --&gt;<br /> <br /> &lt;!-- ==Personal Information== --&gt;<br /> &lt;!-- ==Undergraduate Research Activities== --&gt;<br /> &lt;!-- ==Presentations== --&gt;<br /> &lt;!-- ==Interests== --&gt;<br /> &lt;!-- ==Career Goals== --&gt;<br /> &lt;!-- --&gt;<br /> &lt;!-- --&gt;<br /> <br /> ==Personal Information==<br /> Senior biochemistry major<br /> <br /> Hometown: Peoria, IL<br /> <br /> Contact Info: 309-265-1518; slang@monmouthcollege.edu<br /> <br /> ==Undergraduate Research Activities==<br /> <br /> Aug 2013: SOfIA (2013) The Effects of Sulfadimethoxine and Malachite Green on the Gregarine Parasite Count of Madagascar Hissing Cockroaches<br /> *Studied how different dosages of two drugs, sulfadimethoxine and malachite green, affected the level of gregarine parasite infection in Madagascar Hissing Cockroaches<br /> *Constructed presentation and poster to present findings at the end of the 3-week research period<br /> <br /> Aug 2015: SOfIA (2015) The Effects of Gregarine Parasites on the Physiology of Madagascar Hissing Cockroaches<br /> *Guided freshman SOfIA students on project creation and served as a mentor throughout the project<br /> *Aided in new students' development of lab, research, and presentation skills<br /> <br /> Current: BIOC 430 with Sturgeon<br /> <br /> ==Presentations==<br /> Aug 2013: (SOfIA poster presentation)''The Effects of Sulfadimethoxine and Malachite Green on the Gregarine Parasite Count of Madagascar Hissing Cockroaches''<br /> <br /> Aug 2013: (SOfIA poster presentation)''The Effects of Gregarine Parasites on the Physiology of Madagascar Hissing Cockroaches''<br /> <br /> ==Other Involvement==<br /> <br /> '''Midwest Journal of Undergraduate Research'''<br /> *Publications Editor: Responsible for formatting the final printed journal using InDesign software<br /> *Developed skills in reviewing and editing undergraduate research papers from a variety of disciplines<br /> <br /> '''Teaching Assistant'''<br /> *Managed lab safety and aided students in proper use of instrumentation<br /> *Provided feedback to students on lab calculations and lab technique<br /> <br /> '''Biology 150 SI'''<br /> *Held sessions outside of regular class time to help students understand a variety of topics<br /> *Organized evening review sessions for exams<br /> *Worked with the Biology 150 professor to construct and distribute study guides for each in-class exam<br /> <br /> '''OSF Summer Student Volunteer'''<br /> *30+ hours spent working in the Adult Medical/Surgical Unit<br /> *Aided in replacing shipments of medical supplies, sanitizing stations on the floor, and ensuring patient comfort whenever possible<br /> *Developed skills in bedside manner, recognition of necessary equipment, and hospital navigation.<br /> <br /> '''Monmouth College Swim Team (2013-2015)'''<br /> *Academic All-Conference Athlete (2013-2015)<br /> *Developed skills in both leading and cooperating on a diverse team of athletes<br /> <br /> ==Career Plans==<br /> <br /> Nurse Practitioner (through direct-entry MSN for non-nursing majors)</div>

Sarahalang535 http://205.166.159.208/wiki/index.php?title=Sarah_Lang&diff=2994 2016-09-05T20:28:27Z

<p>Sarahalang535: /* Undergraduate Research Activities */</p> <hr /> <div>&lt;!-- Greetings...this text does not show up on the page since it is &quot;hidden&quot; with the surrounding tags --&gt;<br /> &lt;!-- This is the suggested format for current students to organize their information; this is NOT a requirement --&gt;<br /> &lt;!-- To add this structure to your personal wiki page, copy and paste the code below and then remove the &quot;hidden&quot; tags --&gt;<br /> &lt;!-- ...then of course...add your information. Use Mr. Bitchly's text for coding examples...pls add examples to this profile --&gt;<br /> <br /> &lt;!-- ==Personal Information== --&gt;<br /> &lt;!-- ==Undergraduate Research Activities== --&gt;<br /> &lt;!-- ==Presentations== --&gt;<br /> &lt;!-- ==Interests== --&gt;<br /> &lt;!-- ==Career Goals== --&gt;<br /> &lt;!-- --&gt;<br /> &lt;!-- --&gt;<br /> <br /> ==Personal Information==<br /> Senior biochemistry major<br /> <br /> Hometown: Peoria, IL<br /> <br /> Contact Info: 309-265-1518; slang@monmouthcollege.edu<br /> <br /> ==Undergraduate Research Activities==<br /> <br /> Aug 2013: SOfIA (2013) The Effects of Sulfadimethoxine and Malachite Green on the Gregarine Parasite Count of Madagascar Hissing Cockroaches<br /> *Studied how different dosages of two drugs, sulfadimethoxine and malachite green, affected the level of gregarine parasite infection in Madagascar Hissing Cockroaches<br /> *Constructed presentation and poster to present findings at the end of the 3-week research period<br /> <br /> Aug 2015: SOfIA (2015) The Effects of Gregarine Parasites on the Physiology of Madagascar Hissing Cockroaches<br /> *Guided freshman SOfIA students on project creation and served as a mentor throughout the project<br /> *Aided in new students' development of lab, research, and presentation skills<br /> <br /> Current: BIOC 430 with Sturgeon<br /> <br /> ==Presentations==<br /> Aug 2013: (SOfIA poster presentation)''The Effects of Sulfadimethoxine and Malachite Green on the Gregarine Parasite Count of Madagascar Hissing Cockroaches''<br /> <br /> Aug 2013: (SOfIA poster presentation)''The Effects of Gregarine Parasites on the Physiology of Madagascar Hissing Cockroaches''<br /> <br /> ==Other Involvement==<br /> <br /> '''Midwest Journal of Undergraduate Research'''<br /> *Publications Editor: Responsible for formatting the final printed journal using InDesign software<br /> *Developed skills in reviewing and editing undergraduate research papers from a variety of disciplines<br /> <br /> '''Teaching Assistant'''<br /> *Managed lab safety and aided students in proper use of instrumentation<br /> *Provided feedback to students on lab calculations and lab technique<br /> <br /> '''Biology 150 SI'''<br /> *Held sessions outside of regular class time to help students understand a variety of topics<br /> *Organized evening review sessions for exams<br /> *Worked with the Biology 150 professor to construct and distribute study guides for each in-class exam<br /> <br /> '''OSF Summer Student Volunteer'''<br /> *30+ hours spent working in the Adult Medical/Surgical Unit<br /> *Aided in replacing shipments of medical supplies, sanitizing stations on the floor, and ensuring patient comfort whenever possible<br /> *Developed skills in bedside manner, recognition of necessary equipment, and hospital navigation.<br /> <br /> '''Monmouth College Swim Team (2013-2015)'''<br /> *Academic All-Conference Athlete<br /> *Developed skills in both leading and cooperating on a diverse team of athletes<br /> <br /> ==Career Plans==<br /> <br /> Nurse Practitioner (through direct-entry MSN for non-nursing majors)</div>

Sarahalang535