MC Chem Wiki - User contributions [en]

Riley E Bigham

2022-09-05T19:50:21Z

Rileybigham: /* Presentations */

==Personal Information==
Class of 2023

Senior Biochemistry Major

Hometown: O'Fallon, IL

Phone: 618-207-7391

Email: Rbigham@monmouthcollege.edu

=UNDERGRADUATE RESEARCH ACTIVITIES=

==SOfIA (Summer Opportunities for Intellectual Activities)==
===August 2019===
*'''SOfIA Project''': [http://esr.monmsci.net/wiki/index.php/SOfIA_2019 The Chemistry Makerspace]

==Richard "Doc" Kieft Summer Research==
'''Advisor''': ''Dr. Bradley Sturgeon''
===Summer 2021===
*Developed methods for future microbiome research
*[[Methods_include_Qiagen_DNA_isolation_kits|Methods include Qiagen DNA isolation kits]] (Powerwater, Fecal, and Soil), [[PCR & Sequencing|PCR & Sequencing]]
*Worked with Dr. Laura Moore and her research members
*'''Future Research''': The inhabitants of an aquatic microbiome and their effect on a healthy aquatic ecosystem
*'''Final Presentation''': [[:Media:Microbiome Presentation- Riley Bigham.pptx| Understanding the Aquatic Microbiome]]
::''The microbiome is a unique set of microbes living in a particular environment. The microbes that make up the human microbiome are established at birth and evolve throughout life. These microbes are essential for our well-being by regulating our immune system, synthesizing vitamins, and protecting us against certain disease-causing bacteria. The demand for new research has risen in popularity in the past 20 years allowing new discoveries connecting the microbes to the human condition. An example is the use of fecal transplants for treating obesity. New developments have allowed us to understand cause and effects from the rich community of microbes within different environments. As with the human microbiome, aquatic systems also contain a unique collection of microbes. The goal of this project is to evaluate the microbes present in aquatic systems and to correlate the presences of these microbes to the overall health of the aquatic ecosystems.''

===[https://www.sheddaquarium.org/care-and-conservation/shedd-research/shedd-aquarium-microbiome-project| Shedd Aquarium]===

A few Doc Kieft research students and our advisor, Bradley Sturgeon had the opportunity to take a behind the scenes tour of the Shedd Aquarium microbiome lab. There we met Frank Oliaro, the lab manager of the microbiome project. Here we learned the processes of DNA isolation, PCR, and sequencing. Oiaro also recommended the Earth Microbiome Project page to review the 16s Illumina Amplicon protocol for future PCR and sequencing methods.

The week of September 5th we contacted Frank Oliaro on questions about taking the next step for sequencing. He answered our questions and offered to sequence some of our samples at his lab in the Shedd Aquarium. As of September 13th, he is sequencing our first 11 samples of DNA (Soil, Water, and Fecal).

=Presentations=
*November 2021: '''Local Section ACS Conference'''
:Title: ''Starting a Microbiome Research Project'' (Virtual Oral Presentation)

*June 2021: '''Richard "Doc" Kieft Summer Research Program'''
:Title: ''Understanding the Aquatic Microbiome'' (Oral Presentation)

*August 2019: '''Monmouth College SOfIA Program'''
:Title: ''The Chemistry Makerspace'' (Oral and Poster Presentations)

==Career Goals==

Riley E Bigham

2022-09-05T19:45:54Z

Rileybigham:

==Personal Information==
Class of 2023

Senior Biochemistry Major

Hometown: O'Fallon, IL

Phone: 618-207-7391

Email: Rbigham@monmouthcollege.edu

=UNDERGRADUATE RESEARCH ACTIVITIES=

==SOfIA (Summer Opportunities for Intellectual Activities)==
===August 2019===
*'''SOfIA Project''': [http://esr.monmsci.net/wiki/index.php/SOfIA_2019 The Chemistry Makerspace]

==Richard "Doc" Kieft Summer Research==
'''Advisor''': ''Dr. Bradley Sturgeon''
===Summer 2021===
*Developed methods for future microbiome research
*[[Methods_include_Qiagen_DNA_isolation_kits|Methods include Qiagen DNA isolation kits]] (Powerwater, Fecal, and Soil), [[PCR & Sequencing|PCR & Sequencing]]
*Worked with Dr. Laura Moore and her research members
*'''Future Research''': The inhabitants of an aquatic microbiome and their effect on a healthy aquatic ecosystem
*'''Final Presentation''': [[:Media:Microbiome Presentation- Riley Bigham.pptx| Understanding the Aquatic Microbiome]]
::''The microbiome is a unique set of microbes living in a particular environment. The microbes that make up the human microbiome are established at birth and evolve throughout life. These microbes are essential for our well-being by regulating our immune system, synthesizing vitamins, and protecting us against certain disease-causing bacteria. The demand for new research has risen in popularity in the past 20 years allowing new discoveries connecting the microbes to the human condition. An example is the use of fecal transplants for treating obesity. New developments have allowed us to understand cause and effects from the rich community of microbes within different environments. As with the human microbiome, aquatic systems also contain a unique collection of microbes. The goal of this project is to evaluate the microbes present in aquatic systems and to correlate the presences of these microbes to the overall health of the aquatic ecosystems.''

===[https://www.sheddaquarium.org/care-and-conservation/shedd-research/shedd-aquarium-microbiome-project| Shedd Aquarium]===

A few Doc Kieft research students and our advisor, Bradley Sturgeon had the opportunity to take a behind the scenes tour of the Shedd Aquarium microbiome lab. There we met Frank Oliaro, the lab manager of the microbiome project. Here we learned the processes of DNA isolation, PCR, and sequencing. Oiaro also recommended the Earth Microbiome Project page to review the 16s Illumina Amplicon protocol for future PCR and sequencing methods.

The week of September 5th we contacted Frank Oliaro on questions about taking the next step for sequencing. He answered our questions and offered to sequence some of our samples at his lab in the Shedd Aquarium. As of September 13th, he is sequencing our first 11 samples of DNA (Soil, Water, and Fecal).

=Presentations=
*June 2021: '''Richard "Doc" Kieft Summer Research Program'''
:Title: ''Understanding the Aquatic Microbiome'' (Oral Presentation)

*August 2019: '''Monmouth College SOfIA Program'''
:Title: ''The Chemistry Makerspace'' (Oral and Poster Presentations)

==Career Goals==

Riley E Bigham

2021-09-16T18:08:35Z

Rileybigham: /* Shedd Aquarium */

==Personal Information==
Class of 2023

Junior Biochemistry Major

Hometown: O'Fallon, IL

Phone: 618-207-7391

Email: Rbigham@monmouthcollege.edu

=UNDERGRADUATE RESEARCH ACTIVITIES=

==SOfIA (Summer Opportunities for Intellectual Activities)==
===August 2019===
*'''SOfIA Project''': [http://esr.monmsci.net/wiki/index.php/SOfIA_2019 The Chemistry Makerspace]

==Richard "Doc" Kieft Summer Research==
'''Advisor''': ''Dr. Bradley Sturgeon''
===Summer 2021===
*Developed methods for future microbiome research
*[[Methods_include_Qiagen_DNA_isolation_kits|Methods include Qiagen DNA isolation kits]] (Powerwater, Fecal, and Soil), [[PCR & Sequencing|PCR & Sequencing]]
*Worked with Dr. Laura Moore and her research members
*'''Future Research''': The inhabitants of an aquatic microbiome and their effect on a healthy aquatic ecosystem
*'''Final Presentation''': [[:Media:Microbiome Presentation- Riley Bigham.pptx| Understanding the Aquatic Microbiome]]
::''The microbiome is a unique set of microbes living in a particular environment. The microbes that make up the human microbiome are established at birth and evolve throughout life. These microbes are essential for our well-being by regulating our immune system, synthesizing vitamins, and protecting us against certain disease-causing bacteria. The demand for new research has risen in popularity in the past 20 years allowing new discoveries connecting the microbes to the human condition. An example is the use of fecal transplants for treating obesity. New developments have allowed us to understand cause and effects from the rich community of microbes within different environments. As with the human microbiome, aquatic systems also contain a unique collection of microbes. The goal of this project is to evaluate the microbes present in aquatic systems and to correlate the presences of these microbes to the overall health of the aquatic ecosystems.''

===[https://www.sheddaquarium.org/care-and-conservation/shedd-research/shedd-aquarium-microbiome-project| Shedd Aquarium]===

A few Doc Kieft research students and our advisor, Bradley Sturgeon had the opportunity to take a behind the scenes tour of the Shedd Aquarium microbiome lab. There we met Frank Oliaro, the lab manager of the microbiome project. Here we learned the processes of DNA isolation, PCR, and sequencing. Oiaro also recommended the Earth Microbiome Project page to review the 16s Illumina Amplicon protocol for future PCR and sequencing methods.

The week of September 5th we contacted Frank Oliaro on questions about taking the next step for sequencing. He answered our questions and offered to sequence some of our samples at his lab in the Shedd Aquarium. As of September 13th, he is sequencing our first 11 samples of DNA (Soil, Water, and Fecal).

=Presentations=
*June 2021: '''Richard "Doc" Kieft Summer Research Program'''
:Title: ''Understanding the Aquatic Microbiome'' (Oral Presentation)

*August 2019: '''Monmouth College SOfIA Program'''
:Title: ''The Chemistry Makerspace'' (Oral and Poster Presentations)

==Career Goals==

Methods include Qiagen DNA isolation kits

2021-09-16T17:46:00Z

Rileybigham: /* Qiagen Kit Overview */

== Qiagen Kit Overview==

[https://www.qiagen.com/us/product-categories/discovery-and-translational-research/dna-rna-purification/dna-purification/| QIAGEN]offers molecular sample and assay technology. Some of these technologies are provided as kits. These kits utilize spin columns to isolate DNA and RNA.

'''DNA Extraction'''
Lysis Buffer breaks down the cell membrane to release intercellular materials like DNA, RNA, and other organelles from the cell.

'''Purification'''
To bind the DNA to the silica spin column, Chaotropic Salts are critical. Salts disrupt Hydrogen bonding between strands and allow the DNA to adhere to the silica column.

'''Wash'''
Now that the DNA is bound to the column, it needs to be washed. The membrane is still dirty with residual proteins and salt. Washing it will remove the impurities.

'''Dry'''
After the column is washed it is centrifuged to dry. Drying removes the ethanol and prevents contamination and a low yield.

'''Elution'''
The final step is releasing the pure DNA from the silica column. This is done by allowing 10mM Tris buffer to sit in the DNA before centrifuging.

==DNeasy PowerWater Sterivex Kit==
The kit isolates DNA using sterivex filters without the utilization of enzymes or harsh chemicals.

'''Microbiome Project'''

Using this kit we isolated the DNA of 3 fish tanks from the chemistry and biology wings of the Center of Science and Business. Two of the samples were freshwater and the other, saltwater.

==DNeasy PowerSoil Pro Kit==
The kit produces high yields of microbial DNA from a variety of soil types such as top soil, compost, and clay.

'''Microbiome Project'''

Seven soil samples were isolated using the kit. These samples were pulled from Professor Laura Moore's garden.

==QIAamp PowerFecal Pro DNA Kit==
The kit produces high yields of microbial DNA from stool and gut samples.

'''Microbiome Project'''
Using this kit we isolated DNA from two animals. The first sample was Professor Laura Moore's three month old Labrador retriever. The second sample was from fellow research student, Talia Long's hamster.

Methods include Qiagen DNA isolation kits

2021-09-16T17:45:37Z

Rileybigham: /* Qiagen Kit Overview */

== Qiagen Kit Overview==

[https://www.qiagen.com/us/product-categories/discovery-and-translational-research/dna-rna-purification/dna-purification/|QIAGEN]offers molecular sample and assay technology. Some of these technologies are provided as kits. These kits utilize spin columns to isolate DNA and RNA.

'''DNA Extraction'''
Lysis Buffer breaks down the cell membrane to release intercellular materials like DNA, RNA, and other organelles from the cell.

'''Purification'''
To bind the DNA to the silica spin column, Chaotropic Salts are critical. Salts disrupt Hydrogen bonding between strands and allow the DNA to adhere to the silica column.

'''Wash'''
Now that the DNA is bound to the column, it needs to be washed. The membrane is still dirty with residual proteins and salt. Washing it will remove the impurities.

'''Dry'''
After the column is washed it is centrifuged to dry. Drying removes the ethanol and prevents contamination and a low yield.

'''Elution'''
The final step is releasing the pure DNA from the silica column. This is done by allowing 10mM Tris buffer to sit in the DNA before centrifuging.

==DNeasy PowerWater Sterivex Kit==
The kit isolates DNA using sterivex filters without the utilization of enzymes or harsh chemicals.

'''Microbiome Project'''

Using this kit we isolated the DNA of 3 fish tanks from the chemistry and biology wings of the Center of Science and Business. Two of the samples were freshwater and the other, saltwater.

==DNeasy PowerSoil Pro Kit==
The kit produces high yields of microbial DNA from a variety of soil types such as top soil, compost, and clay.

'''Microbiome Project'''

Seven soil samples were isolated using the kit. These samples were pulled from Professor Laura Moore's garden.

==QIAamp PowerFecal Pro DNA Kit==
The kit produces high yields of microbial DNA from stool and gut samples.

'''Microbiome Project'''
Using this kit we isolated DNA from two animals. The first sample was Professor Laura Moore's three month old Labrador retriever. The second sample was from fellow research student, Talia Long's hamster.

Methods include Qiagen DNA isolation kits

2021-09-16T17:45:06Z

Rileybigham: /* Qiagen Kit Overview */

== Qiagen Kit Overview==

[QIAGEN|https://www.qiagen.com/us/product-categories/discovery-and-translational-research/dna-rna-purification/dna-purification/]offers molecular sample and assay technology. Some of these technologies are provided as kits. These kits utilize spin columns to isolate DNA and RNA.

'''DNA Extraction'''
Lysis Buffer breaks down the cell membrane to release intercellular materials like DNA, RNA, and other organelles from the cell.

'''Purification'''
To bind the DNA to the silica spin column, Chaotropic Salts are critical. Salts disrupt Hydrogen bonding between strands and allow the DNA to adhere to the silica column.

'''Wash'''
Now that the DNA is bound to the column, it needs to be washed. The membrane is still dirty with residual proteins and salt. Washing it will remove the impurities.

'''Dry'''
After the column is washed it is centrifuged to dry. Drying removes the ethanol and prevents contamination and a low yield.

'''Elution'''
The final step is releasing the pure DNA from the silica column. This is done by allowing 10mM Tris buffer to sit in the DNA before centrifuging.

==DNeasy PowerWater Sterivex Kit==
The kit isolates DNA using sterivex filters without the utilization of enzymes or harsh chemicals.

'''Microbiome Project'''

Using this kit we isolated the DNA of 3 fish tanks from the chemistry and biology wings of the Center of Science and Business. Two of the samples were freshwater and the other, saltwater.

==DNeasy PowerSoil Pro Kit==
The kit produces high yields of microbial DNA from a variety of soil types such as top soil, compost, and clay.

'''Microbiome Project'''

Seven soil samples were isolated using the kit. These samples were pulled from Professor Laura Moore's garden.

==QIAamp PowerFecal Pro DNA Kit==
The kit produces high yields of microbial DNA from stool and gut samples.

'''Microbiome Project'''
Using this kit we isolated DNA from two animals. The first sample was Professor Laura Moore's three month old Labrador retriever. The second sample was from fellow research student, Talia Long's hamster.

Riley E Bigham

2021-09-16T17:41:20Z

Rileybigham: /* [|Shedd Aquarium */

==Personal Information==
Class of 2023

Junior Biochemistry Major

Hometown: O'Fallon, IL

Phone: 618-207-7391

Email: Rbigham@monmouthcollege.edu

=UNDERGRADUATE RESEARCH ACTIVITIES=

==SOfIA (Summer Opportunities for Intellectual Activities)==
===August 2019===
*'''SOfIA Project''': [http://esr.monmsci.net/wiki/index.php/SOfIA_2019 The Chemistry Makerspace]

==Richard "Doc" Kieft Summer Research==
'''Advisor''': ''Dr. Bradley Sturgeon''
===Summer 2021===
*Developed methods for future microbiome research
*[[Methods_include_Qiagen_DNA_isolation_kits|Methods include Qiagen DNA isolation kits]] (Powerwater, Fecal, and Soil), [[PCR & Sequencing|PCR & Sequencing]]
*Worked with Dr. Laura Moore and her research members
*'''Future Research''': The inhabitants of an aquatic microbiome and their effect on a healthy aquatic ecosystem
*'''Final Presentation''': [[:Media:Microbiome Presentation- Riley Bigham.pptx| Understanding the Aquatic Microbiome]]
::''The microbiome is a unique set of microbes living in a particular environment. The microbes that make up the human microbiome are established at birth and evolve throughout life. These microbes are essential for our well-being by regulating our immune system, synthesizing vitamins, and protecting us against certain disease-causing bacteria. The demand for new research has risen in popularity in the past 20 years allowing new discoveries connecting the microbes to the human condition. An example is the use of fecal transplants for treating obesity. New developments have allowed us to understand cause and effects from the rich community of microbes within different environments. As with the human microbiome, aquatic systems also contain a unique collection of microbes. The goal of this project is to evaluate the microbes present in aquatic systems and to correlate the presences of these microbes to the overall health of the aquatic ecosystems.''

===[https://www.sheddaquarium.org/care-and-conservation/shedd-research/shedd-aquarium-microbiome-project| Shedd Aquarium]===

A few Doc Kieft research students and our advisor, Bradley Sturgeon had the opportunity to take a behind the scenes tour of the Shedd Aquarium microbiome lab. There we met Frank Oliaro, the lab manager of the microbiome project.

=Presentations=
*June 2021: '''Richard "Doc" Kieft Summer Research Program'''
:Title: ''Understanding the Aquatic Microbiome'' (Oral Presentation)

*August 2019: '''Monmouth College SOfIA Program'''
:Title: ''The Chemistry Makerspace'' (Oral and Poster Presentations)

==Career Goals==

Riley E Bigham

2021-09-16T17:40:11Z

Rileybigham:

==Personal Information==
Class of 2023

Junior Biochemistry Major

Hometown: O'Fallon, IL

Phone: 618-207-7391

Email: Rbigham@monmouthcollege.edu

=UNDERGRADUATE RESEARCH ACTIVITIES=

==SOfIA (Summer Opportunities for Intellectual Activities)==
===August 2019===
*'''SOfIA Project''': [http://esr.monmsci.net/wiki/index.php/SOfIA_2019 The Chemistry Makerspace]

==Richard "Doc" Kieft Summer Research==
'''Advisor''': ''Dr. Bradley Sturgeon''
===Summer 2021===
*Developed methods for future microbiome research
*[[Methods_include_Qiagen_DNA_isolation_kits|Methods include Qiagen DNA isolation kits]] (Powerwater, Fecal, and Soil), [[PCR & Sequencing|PCR & Sequencing]]
*Worked with Dr. Laura Moore and her research members
*'''Future Research''': The inhabitants of an aquatic microbiome and their effect on a healthy aquatic ecosystem
*'''Final Presentation''': [[:Media:Microbiome Presentation- Riley Bigham.pptx| Understanding the Aquatic Microbiome]]
::''The microbiome is a unique set of microbes living in a particular environment. The microbes that make up the human microbiome are established at birth and evolve throughout life. These microbes are essential for our well-being by regulating our immune system, synthesizing vitamins, and protecting us against certain disease-causing bacteria. The demand for new research has risen in popularity in the past 20 years allowing new discoveries connecting the microbes to the human condition. An example is the use of fecal transplants for treating obesity. New developments have allowed us to understand cause and effects from the rich community of microbes within different environments. As with the human microbiome, aquatic systems also contain a unique collection of microbes. The goal of this project is to evaluate the microbes present in aquatic systems and to correlate the presences of these microbes to the overall health of the aquatic ecosystems.''

===[|[https://www.sheddaquarium.org/care-and-conservation/shedd-research/shedd-aquarium-microbiome-project| Shedd Aquarium]===

A few Doc Kieft research students and our advisor, Bradley Sturgeon had the opportunity to take a behind the scenes tour of the Shedd Aquarium microbiome lab. There we met Frank Oliaro, the lab manager of the microbiome project.

=Presentations=
*June 2021: '''Richard "Doc" Kieft Summer Research Program'''
:Title: ''Understanding the Aquatic Microbiome'' (Oral Presentation)

*August 2019: '''Monmouth College SOfIA Program'''
:Title: ''The Chemistry Makerspace'' (Oral and Poster Presentations)

==Career Goals==

Riley E Bigham

2021-09-16T17:35:06Z

Rileybigham: /* Shedd Aquarium */

==Personal Information==
Class of 2023

Junior Biochemistry Major

Hometown: O'Fallon, IL

Phone: 618-207-7391

Email: Rbigham@monmouthcollege.edu

=UNDERGRADUATE RESEARCH ACTIVITIES=

==SOfIA (Summer Opportunities for Intellectual Activities)==
===August 2019===
*'''SOfIA Project''': [http://esr.monmsci.net/wiki/index.php/SOfIA_2019 The Chemistry Makerspace]

==Richard "Doc" Kieft Summer Research==
'''Advisor''': ''Dr. Bradley Sturgeon''
===Summer 2021===
*Developed methods for future microbiome research
*[[Methods_include_Qiagen_DNA_isolation_kits|Methods include Qiagen DNA isolation kits]] (Powerwater, Fecal, and Soil), [[PCR & Sequencing|PCR & Sequencing]]
*Worked with Dr. Laura Moore and her research members
*'''Future Research''': The inhabitants of an aquatic microbiome and their effect on a healthy aquatic ecosystem
*'''Final Presentation''': [[:Media:Microbiome Presentation- Riley Bigham.pptx| Understanding the Aquatic Microbiome]]
::''The microbiome is a unique set of microbes living in a particular environment. The microbes that make up the human microbiome are established at birth and evolve throughout life. These microbes are essential for our well-being by regulating our immune system, synthesizing vitamins, and protecting us against certain disease-causing bacteria. The demand for new research has risen in popularity in the past 20 years allowing new discoveries connecting the microbes to the human condition. An example is the use of fecal transplants for treating obesity. New developments have allowed us to understand cause and effects from the rich community of microbes within different environments. As with the human microbiome, aquatic systems also contain a unique collection of microbes. The goal of this project is to evaluate the microbes present in aquatic systems and to correlate the presences of these microbes to the overall health of the aquatic ecosystems.''

===Shedd Aquarium===

A few Doc Kieft research students and our advisor, Bradley Sturgeon had the opportunity to take a behind the scenes tour of the Shedd Aquarium microbiome lab. There we met Frank Oliaro, the lab manager of the microbiome project.

=Presentations=
*June 2021: '''Richard "Doc" Kieft Summer Research Program'''
:Title: ''Understanding the Aquatic Microbiome'' (Oral Presentation)

*August 2019: '''Monmouth College SOfIA Program'''
:Title: ''The Chemistry Makerspace'' (Oral and Poster Presentations)

==Career Goals==

PCR & Sequencing

2021-09-16T17:28:28Z

Rileybigham:

'''Polymerase chain reaction (PCR)'''

A commonly used method to make millions of genetic copies of specific DNA samples. This allows scientists to amplify small samples of DNA and make them large enough to analyze.

'''DNA Sequencing'''

A process used to determine the sequence of nucleotides in a DNA sample. There are two types of sequencing, Sanger Sequencing and Next-generation Sequencing. Sequencing breaks the genome of a DNA into smaller segments, sequencing those segments and assembling the sequences into one long strand.

Sanger sequencing copies the target DNA and make fragments of different lengths. Fluorescent nucleotides mark the ends of the fragments and allow the sequence to be determined.

Next-generation sequencing are new methods for sequencing. This approach increases the speed and is cost-effective.

==How does PCR work?==
To amplify the sample of DNA, the sample is first heated so the DNA separates into two single stranded DNA. An enzyme called Taq polymerase builds two new strands of DNA. The two original strands of DNA act as templates for the enzyme. Each of the strands are then used to continually create two new copies. The separation and synthesizing process repeats as many as 30 times. This makes over one billion copies of the original DNA segments.

The PCR process uses an automated thermocycler that is programmed to alter the temperature of the reaction every few minutes to allow for DNA separation and synthesis.

==How does sequencing work==
'''Sanger Sequencing'''

[[File:Sanger Sequencing.png|thumbnail|Sanger Sequencing]]
Fragments of DNA are aligned by their overlapping portions to assemble the sequences of larger DNA regions and later, entire chromosomes. This process is still popularly used today to sequence individual DNA pieces used for cloning or through PCR.

'''Next-generation sequencing'''
Next-gen sequencing is like running multiple small sanger sequencing reactions at the same time. This allows sequencing to be faster and cheaper than Sanger.

PCR & Sequencing

2021-09-16T17:27:46Z

Rileybigham: /* How does sequencing work */

'''Polymerase chain reaction (PCR)'''
A commonly used method to make millions of genetic copies of specific DNA samples. This allows scientists to amplify small samples of DNA and make them large enough to analyze.

'''DNA Sequencing'''
A process used to determine the sequence of nucleotides in a DNA sample. There are two types of sequencing, Sanger Sequencing and Next-generation Sequencing. Sequencing breaks the genome of a DNA into smaller segments, sequencing those segments and assembling the sequences into one long strand.

Sanger sequencing copies the target DNA and make fragments of different lengths. Fluorescent nucleotides mark the ends of the fragments and allow the sequence to be determined.

Next-generation sequencing are new methods for sequencing. This approach increases the speed and is cost-effective.

==How does PCR work?==
To amplify the sample of DNA, the sample is first heated so the DNA separates into two single stranded DNA. An enzyme called Taq polymerase builds two new strands of DNA. The two original strands of DNA act as templates for the enzyme. Each of the strands are then used to continually create two new copies. The separation and synthesizing process repeats as many as 30 times. This makes over one billion copies of the original DNA segments.

The PCR process uses an automated thermocycler that is programmed to alter the temperature of the reaction every few minutes to allow for DNA separation and synthesis.

==How does sequencing work==
'''Sanger Sequencing'''

[[File:Sanger Sequencing.png|thumbnail|Sanger Sequencing]]
Fragments of DNA are aligned by their overlapping portions to assemble the sequences of larger DNA regions and later, entire chromosomes. This process is still popularly used today to sequence individual DNA pieces used for cloning or through PCR.

'''Next-generation sequencing'''
Next-gen sequencing is like running multiple small sanger sequencing reactions at the same time. This allows sequencing to be faster and cheaper than Sanger.

PCR & Sequencing

2021-09-16T17:27:22Z

Rileybigham: /* How does sequencing work */

'''Polymerase chain reaction (PCR)'''
A commonly used method to make millions of genetic copies of specific DNA samples. This allows scientists to amplify small samples of DNA and make them large enough to analyze.

'''DNA Sequencing'''
A process used to determine the sequence of nucleotides in a DNA sample. There are two types of sequencing, Sanger Sequencing and Next-generation Sequencing. Sequencing breaks the genome of a DNA into smaller segments, sequencing those segments and assembling the sequences into one long strand.

Sanger sequencing copies the target DNA and make fragments of different lengths. Fluorescent nucleotides mark the ends of the fragments and allow the sequence to be determined.

Next-generation sequencing are new methods for sequencing. This approach increases the speed and is cost-effective.

==How does PCR work?==
To amplify the sample of DNA, the sample is first heated so the DNA separates into two single stranded DNA. An enzyme called Taq polymerase builds two new strands of DNA. The two original strands of DNA act as templates for the enzyme. Each of the strands are then used to continually create two new copies. The separation and synthesizing process repeats as many as 30 times. This makes over one billion copies of the original DNA segments.

The PCR process uses an automated thermocycler that is programmed to alter the temperature of the reaction every few minutes to allow for DNA separation and synthesis.

==How does sequencing work==
'''Sanger Sequencing'''
Fragments of DNA are aligned by their overlapping portions to assemble the sequences of larger DNA regions and later, entire chromosomes. This process is still popularly used today to sequence individual DNA pieces used for cloning or through PCR.[[File:Sanger Sequencing.png|thumbnail|Sanger Sequencing]]

'''Next-generation sequencing'''
Next-gen sequencing is like running multiple small sanger sequencing reactions at the same time. This allows sequencing to be faster and cheaper than Sanger.

File:Sanger Sequencing.png

2021-09-16T17:26:51Z

Rileybigham:

PCR & Sequencing

2021-09-16T17:26:11Z

Rileybigham:

'''Polymerase chain reaction (PCR)'''
A commonly used method to make millions of genetic copies of specific DNA samples. This allows scientists to amplify small samples of DNA and make them large enough to analyze.

'''DNA Sequencing'''
A process used to determine the sequence of nucleotides in a DNA sample. There are two types of sequencing, Sanger Sequencing and Next-generation Sequencing. Sequencing breaks the genome of a DNA into smaller segments, sequencing those segments and assembling the sequences into one long strand.

Sanger sequencing copies the target DNA and make fragments of different lengths. Fluorescent nucleotides mark the ends of the fragments and allow the sequence to be determined.

Next-generation sequencing are new methods for sequencing. This approach increases the speed and is cost-effective.

==How does PCR work?==
To amplify the sample of DNA, the sample is first heated so the DNA separates into two single stranded DNA. An enzyme called Taq polymerase builds two new strands of DNA. The two original strands of DNA act as templates for the enzyme. Each of the strands are then used to continually create two new copies. The separation and synthesizing process repeats as many as 30 times. This makes over one billion copies of the original DNA segments.

The PCR process uses an automated thermocycler that is programmed to alter the temperature of the reaction every few minutes to allow for DNA separation and synthesis.

==How does sequencing work==
'''Sanger Sequencing'''
Fragments of DNA are aligned by their overlapping portions to assemble the sequences of larger DNA regions and later, entire chromosomes. This process is still popularly used today to sequence individual DNA pieces used for cloning or through PCR.
[[File:Sanger Sequencing.png|thumbnail|Sanger Sequencing]]

'''Next-generation sequencing'''
Next-gen sequencing is like running multiple small sanger sequencing reactions at the same time. This allows sequencing to be faster and cheaper than Sanger.

PCR & Sequencing

2021-09-16T17:05:09Z

Rileybigham:

'''Polymerase chain reaction (PCR)'''
A commonly used method to make millions of genetic copies of specific DNA samples. This allows scientists to amplify small samples of DNA and make them large enough to analyze.

'''DNA Sequencing'''
A process used to determine the sequence of nucleotides in a DNA sample. There are two types of sequencing, '''Sanger Sequencing''' and '''Next-generation Sequencing'''. Sequencing breaks the genome of a DNA into smaller segments, sequencing those segments and assembling the sequences into one long strand.

Sanger sequencing copies the target DNA and make fragments of different lengths. Fluorescent nucleotides mark the ends of the fragments and allow the sequence to be determined.

Next-generation sequencing are new methods for sequencing. This approach increases the speed and is cost-effective.

==How does PCR work?==
To amplify the sample of DNA, the sample is first heated so the DNA separates into two single stranded DNA. An enzyme called Taq polymerase builds two new strands of DNA. The two original strands of DNA act as templates for the enzyme. Each of the strands are then used to continually create two new copies. The separation and synthesizing process repeats as many as 30 times. This makes over one billion copies of the original DNA segments.

The PCR process uses an automated thermocycler that is programmed to alter the temperature of the reaction every few minutes to allow for DNA separation and synthesis.

PCR & Sequencing

2021-09-16T16:43:12Z

Rileybigham: /* How does PCR work? */

'''Polymerase chain reaction (PCR)'''
A commonly used method to make millions of genetic copies of specific DNA samples. This allows scientists to amplify small samples of DNA and make them large enough to analyze.
==How does PCR work?==
To amplify the sample of DNA, the sample is first heated so the DNA separates into two single stranded DNA. An enzyme called Taq polymerase builds two new strands of DNA. The two original strands of DNA act as templates for the enzyme. Each of the strands are then used to continually create two new copies. The separation and synthesizing process repeats as many as 30 times. This makes over one billion copies of the original DNA segments.

The PCR process uses an automated thermocycler that is programmed to alter the temperature of the reaction every few minutes to allow for DNA separation and synthesis.

PCR & Sequencing

2021-09-16T16:41:34Z

Rileybigham: /* How does PCR work? */

'''Polymerase chain reaction (PCR)'''
A commonly used method to make millions of genetic copies of specific DNA samples. This allows scientists to amplify small samples of DNA and make them large enough to analyze.
==How does PCR work?==
To amplify the sample of DNA, the sample is first heated so the DNA separates into two single stranded DNA. An enzyme called Taq polymerase builds two new strands of DNA. The two original strands of DNA act as templates for the enzyme. Each of the strands are then used to continually create two new copies. The separation and synthesizing process repeats as many as 30 times. This makes over one billion copies of the original DNA segments.
The PCR process uses an automated thermocycler that is programmed to alter the temperature of the reaction every few minutes to allow for DNA separation and synthesis.

PCR & Sequencing

2021-09-16T16:41:20Z

Rileybigham: /* How does PCR work? */

'''Polymerase chain reaction (PCR)'''
A commonly used method to make millions of genetic copies of specific DNA samples. This allows scientists to amplify small samples of DNA and make them large enough to analyze.
==How does PCR work?==
To amplify the sample of DNA, the sample is first heated so the DNA separates into two single stranded DNA. An enzyme called Taq polymerase builds two new strands of DNA. The two original strands of DNA act as templates for the enzyme. Each of the strands are then used to continually create two new copies. The separation and synthesizing process repeats as many as 30 times. This makes over one billion copies of the original DNA segments.
The PCR process uses an automated thermocycler that is programmed to alter the temperature of the reaction every few minutes to allow for DNA separation and synthesis.

PCR & Sequencing

2021-09-16T16:40:31Z

Rileybigham:

'''Polymerase chain reaction (PCR)'''
A commonly used method to make millions of genetic copies of specific DNA samples. This allows scientists to amplify small samples of DNA and make them large enough to analyze.
==How does PCR work?==
To amplify the sample of DNA, the sample is first heated so
the DNA separates into two single stranded DNA. An enzyme called Taq polymerase builds two new strands of DNA. The two original strands of DNA act as templates for the enzyme. Each of the strands are then used to continually create two new copies. The separation and synthesizing process repeats as many as 30 times. This makes over one billion copies of the original DNA segments.
The PCR process uses an automated thermocycler that is programmed to alter the temperature of the reaction every few minutes to allow for DNA separation and synthesis.

PCR & Sequencing

2021-09-16T16:11:24Z

Rileybigham: Created page with "'''Polymerase chain reaction (PCR)''' A commonly used method to make millions of genetic copies of specific DNA samples. This allows scientists to amplify small samples of DNA..."

Methods include Qiagen DNA isolation kits

2021-09-16T16:06:37Z

Rileybigham: /* QIAamp PowerFecal Pro DNA Kit */

== Qiagen Kit Overview==

QIAGEN offers molecular sample and assay technology. Some of these technologies are provided as kits. These kits utilize spin columns to isolate DNA and RNA.

'''DNA Extraction'''
Lysis Buffer breaks down the cell membrane to release intercellular materials like DNA, RNA, and other organelles from the cell.

'''Purification'''
To bind the DNA to the silica spin column, Chaotropic Salts are critical. Salts disrupt Hydrogen bonding between strands and allow the DNA to adhere to the silica column.

'''Wash'''
Now that the DNA is bound to the column, it needs to be washed. The membrane is still dirty with residual proteins and salt. Washing it will remove the impurities.

'''Dry'''
After the column is washed it is centrifuged to dry. Drying removes the ethanol and prevents contamination and a low yield.

'''Elution'''
The final step is releasing the pure DNA from the silica column. This is done by allowing 10mM Tris buffer to sit in the DNA before centrifuging.

==DNeasy PowerWater Sterivex Kit==
The kit isolates DNA using sterivex filters without the utilization of enzymes or harsh chemicals.

'''Microbiome Project'''

Using this kit we isolated the DNA of 3 fish tanks from the chemistry and biology wings of the Center of Science and Business. Two of the samples were freshwater and the other, saltwater.

==DNeasy PowerSoil Pro Kit==
The kit produces high yields of microbial DNA from a variety of soil types such as top soil, compost, and clay.

'''Microbiome Project'''

Seven soil samples were isolated using the kit. These samples were pulled from Professor Laura Moore's garden.

==QIAamp PowerFecal Pro DNA Kit==
The kit produces high yields of microbial DNA from stool and gut samples.

'''Microbiome Project'''
Using this kit we isolated DNA from two animals. The first sample was Professor Laura Moore's three month old Labrador retriever. The second sample was from fellow research student, Talia Long's hamster.

Methods include Qiagen DNA isolation kits

2021-09-16T16:06:25Z

Rileybigham: /* DNeasy PowerWater Sterivex Kit */

== Qiagen Kit Overview==

QIAGEN offers molecular sample and assay technology. Some of these technologies are provided as kits. These kits utilize spin columns to isolate DNA and RNA.

'''DNA Extraction'''
Lysis Buffer breaks down the cell membrane to release intercellular materials like DNA, RNA, and other organelles from the cell.

'''Purification'''
To bind the DNA to the silica spin column, Chaotropic Salts are critical. Salts disrupt Hydrogen bonding between strands and allow the DNA to adhere to the silica column.

'''Wash'''
Now that the DNA is bound to the column, it needs to be washed. The membrane is still dirty with residual proteins and salt. Washing it will remove the impurities.

'''Dry'''
After the column is washed it is centrifuged to dry. Drying removes the ethanol and prevents contamination and a low yield.

'''Elution'''
The final step is releasing the pure DNA from the silica column. This is done by allowing 10mM Tris buffer to sit in the DNA before centrifuging.

==DNeasy PowerWater Sterivex Kit==
The kit isolates DNA using sterivex filters without the utilization of enzymes or harsh chemicals.

'''Microbiome Project'''

Using this kit we isolated the DNA of 3 fish tanks from the chemistry and biology wings of the Center of Science and Business. Two of the samples were freshwater and the other, saltwater.

==DNeasy PowerSoil Pro Kit==
The kit produces high yields of microbial DNA from a variety of soil types such as top soil, compost, and clay.

'''Microbiome Project'''

Seven soil samples were isolated using the kit. These samples were pulled from Professor Laura Moore's garden.

==QIAamp PowerFecal Pro DNA Kit==

Kit produces high yields of microbial DNA from stool and gut samples.

'''Microbiome Project'''
Using this kit we isolated DNA from two animals. The first sample was Professor Laura Moore's three month old Labrador retriever. The second sample was from fellow research student, Talia Long's hamster.

Methods include Qiagen DNA isolation kits

2021-09-16T16:06:12Z

Rileybigham: /* QIAamp PowerFecal Pro DNA Kit */

== Qiagen Kit Overview==

QIAGEN offers molecular sample and assay technology. Some of these technologies are provided as kits. These kits utilize spin columns to isolate DNA and RNA.

'''DNA Extraction'''
Lysis Buffer breaks down the cell membrane to release intercellular materials like DNA, RNA, and other organelles from the cell.

'''Purification'''
To bind the DNA to the silica spin column, Chaotropic Salts are critical. Salts disrupt Hydrogen bonding between strands and allow the DNA to adhere to the silica column.

'''Wash'''
Now that the DNA is bound to the column, it needs to be washed. The membrane is still dirty with residual proteins and salt. Washing it will remove the impurities.

'''Dry'''
After the column is washed it is centrifuged to dry. Drying removes the ethanol and prevents contamination and a low yield.

'''Elution'''
The final step is releasing the pure DNA from the silica column. This is done by allowing 10mM Tris buffer to sit in the DNA before centrifuging.

==DNeasy PowerWater Sterivex Kit==
Kit isolates DNA using sterivex filters without the utilization of enzymes or harsh chemicals.

'''Microbiome Project'''

Using this kit we isolated the DNA of 3 fish tanks from the chemistry and biology wings of the Center of Science and Business. Two of the samples were freshwater and the other, saltwater.

==DNeasy PowerSoil Pro Kit==
The kit produces high yields of microbial DNA from a variety of soil types such as top soil, compost, and clay.

'''Microbiome Project'''

Seven soil samples were isolated using the kit. These samples were pulled from Professor Laura Moore's garden.

==QIAamp PowerFecal Pro DNA Kit==

Kit produces high yields of microbial DNA from stool and gut samples.

'''Microbiome Project'''
Using this kit we isolated DNA from two animals. The first sample was Professor Laura Moore's three month old Labrador retriever. The second sample was from fellow research student, Talia Long's hamster.

Methods include Qiagen DNA isolation kits

2021-09-16T16:06:00Z

Rileybigham: /* QIAamp PowerFecal Pro DNA Kit */

== Qiagen Kit Overview==

QIAGEN offers molecular sample and assay technology. Some of these technologies are provided as kits. These kits utilize spin columns to isolate DNA and RNA.

'''DNA Extraction'''
Lysis Buffer breaks down the cell membrane to release intercellular materials like DNA, RNA, and other organelles from the cell.

'''Purification'''
To bind the DNA to the silica spin column, Chaotropic Salts are critical. Salts disrupt Hydrogen bonding between strands and allow the DNA to adhere to the silica column.

'''Wash'''
Now that the DNA is bound to the column, it needs to be washed. The membrane is still dirty with residual proteins and salt. Washing it will remove the impurities.

'''Dry'''
After the column is washed it is centrifuged to dry. Drying removes the ethanol and prevents contamination and a low yield.

'''Elution'''
The final step is releasing the pure DNA from the silica column. This is done by allowing 10mM Tris buffer to sit in the DNA before centrifuging.

==DNeasy PowerWater Sterivex Kit==
Kit isolates DNA using sterivex filters without the utilization of enzymes or harsh chemicals.

'''Microbiome Project'''

Using this kit we isolated the DNA of 3 fish tanks from the chemistry and biology wings of the Center of Science and Business. Two of the samples were freshwater and the other, saltwater.

==DNeasy PowerSoil Pro Kit==
The kit produces high yields of microbial DNA from a variety of soil types such as top soil, compost, and clay.

'''Microbiome Project'''

Seven soil samples were isolated using the kit. These samples were pulled from Professor Laura Moore's garden.

==QIAamp PowerFecal Pro DNA Kit==
Kit produces high yields of microbial DNA from stool and gut samples.

'''Microbiome Project'''
Using this kit we isolated DNA from two animals. The first sample was Professor Laura Moore's three month old Labrador retriever. The second sample was from fellow research student, Talia Long's hamster.

Methods include Qiagen DNA isolation kits

2021-09-16T16:03:09Z

Rileybigham:

== Qiagen Kit Overview==

QIAGEN offers molecular sample and assay technology. Some of these technologies are provided as kits. These kits utilize spin columns to isolate DNA and RNA.

'''DNA Extraction'''
Lysis Buffer breaks down the cell membrane to release intercellular materials like DNA, RNA, and other organelles from the cell.

'''Purification'''
To bind the DNA to the silica spin column, Chaotropic Salts are critical. Salts disrupt Hydrogen bonding between strands and allow the DNA to adhere to the silica column.

'''Wash'''
Now that the DNA is bound to the column, it needs to be washed. The membrane is still dirty with residual proteins and salt. Washing it will remove the impurities.

'''Dry'''
After the column is washed it is centrifuged to dry. Drying removes the ethanol and prevents contamination and a low yield.

'''Elution'''
The final step is releasing the pure DNA from the silica column. This is done by allowing 10mM Tris buffer to sit in the DNA before centrifuging.

==DNeasy PowerWater Sterivex Kit==
Kit isolates DNA using sterivex filters without the utilization of enzymes or harsh chemicals.

'''Microbiome Project'''

Using this kit we isolated the DNA of 3 fish tanks from the chemistry and biology wings of the Center of Science and Business. Two of the samples were freshwater and the other, saltwater.

==DNeasy PowerSoil Pro Kit==
The kit produces high yields of microbial DNA from a variety of soil types such as top soil, compost, and clay.

'''Microbiome Project'''

Seven soil samples were isolated using the kit. These samples were pulled from Professor Laura Moore's garden.

==QIAamp PowerFecal Pro DNA Kit==
Kit produces high yields of microbial DNA from stool and gut samples.

'''Microbiome Project'''

Methods include Qiagen DNA isolation kits

2021-09-16T16:01:45Z

Rileybigham: /* DNeasy PowerWater Sterivex Kit */

== Qiagen Kit Overview==

QIAGEN offers molecular sample and assay technology. Some of these technologies are provided as kits. These kits utilize spin columns to isolate DNA and RNA.

'''DNA Extraction'''
Lysis Buffer breaks down the cell membrane to release intercellular materials like DNA, RNA, and other organelles from the cell.

'''Purification'''
To bind the DNA to the silica spin column, Chaotropic Salts are critical. Salts disrupt Hydrogen bonding between strands and allow the DNA to adhere to the silica column.

'''Wash'''
Now that the DNA is bound to the column, it needs to be washed. The membrane is still dirty with residual proteins and salt. Washing it will remove the impurities.

'''Dry'''
After the column is washed it is centrifuged to dry. Drying removes the ethanol and prevents contamination and a low yield.

'''Elution'''
The final step is releasing the pure DNA from the silica column. This is done by allowing 10mM Tris buffer to sit in the DNA before centrifuging.

==DNeasy PowerWater Sterivex Kit==
Kit isolates DNA using sterivex filters without the utilization of enzymes or harsh chemicals.

'''Microbiome Project'''

Using this kit we isolated the DNA of 3 fish tanks from the chemistry and biology wings of the Center of Science and Business. Two of the samples were freshwater and the other, saltwater.

==DNeasy PowerSoil Pro Kit==
The kit produces high yields of microbial DNA from a variety of soil types such as top soil, compost, and clay.

'''Microbiome Project'''

==QIAamp PowerFecal Pro DNA Kit==
Kit produces high yields of microbial DNA from stool and gut samples.

'''Microbiome Project'''

Methods include Qiagen DNA isolation kits

2021-09-16T16:01:32Z

Rileybigham: /* DNeasy PowerWater Sterivex Kit */

== Qiagen Kit Overview==

QIAGEN offers molecular sample and assay technology. Some of these technologies are provided as kits. These kits utilize spin columns to isolate DNA and RNA.

'''DNA Extraction'''
Lysis Buffer breaks down the cell membrane to release intercellular materials like DNA, RNA, and other organelles from the cell.

'''Purification'''
To bind the DNA to the silica spin column, Chaotropic Salts are critical. Salts disrupt Hydrogen bonding between strands and allow the DNA to adhere to the silica column.

'''Wash'''
Now that the DNA is bound to the column, it needs to be washed. The membrane is still dirty with residual proteins and salt. Washing it will remove the impurities.

'''Dry'''
After the column is washed it is centrifuged to dry. Drying removes the ethanol and prevents contamination and a low yield.

'''Elution'''
The final step is releasing the pure DNA from the silica column. This is done by allowing 10mM Tris buffer to sit in the DNA before centrifuging.

==DNeasy PowerWater Sterivex Kit==
Kit isolates DNA using sterivex filters without the utilization of enzymes or harsh chemicals.

'''Microbiome Project'''
Using this kit we isolated the DNA of 3 fish tanks from the chemistry and biology wings of the Center of Science and Business. Two of the samples were freshwater and the other, saltwater.

==DNeasy PowerSoil Pro Kit==
The kit produces high yields of microbial DNA from a variety of soil types such as top soil, compost, and clay.

'''Microbiome Project'''

==QIAamp PowerFecal Pro DNA Kit==
Kit produces high yields of microbial DNA from stool and gut samples.

'''Microbiome Project'''

Methods include Qiagen DNA isolation kits

2021-09-16T15:57:54Z

Rileybigham: /* DNeasy PowerWater Sterivex Kit */

== Qiagen Kit Overview==

QIAGEN offers molecular sample and assay technology. Some of these technologies are provided as kits. These kits utilize spin columns to isolate DNA and RNA.

'''DNA Extraction'''
Lysis Buffer breaks down the cell membrane to release intercellular materials like DNA, RNA, and other organelles from the cell.

'''Purification'''
To bind the DNA to the silica spin column, Chaotropic Salts are critical. Salts disrupt Hydrogen bonding between strands and allow the DNA to adhere to the silica column.

'''Wash'''
Now that the DNA is bound to the column, it needs to be washed. The membrane is still dirty with residual proteins and salt. Washing it will remove the impurities.

'''Dry'''
After the column is washed it is centrifuged to dry. Drying removes the ethanol and prevents contamination and a low yield.

'''Elution'''
The final step is releasing the pure DNA from the silica column. This is done by allowing 10mM Tris buffer to sit in the DNA before centrifuging.

==DNeasy PowerWater Sterivex Kit==
Kit isolates DNA using sterivex filters without the utilization of enzymes or harsh chemicals.

'''Microbiome Project'''

==DNeasy PowerSoil Pro Kit==
The kit produces high yields of microbial DNA from a variety of soil types such as top soil, compost, and clay.

'''Microbiome Project'''

==QIAamp PowerFecal Pro DNA Kit==
Kit produces high yields of microbial DNA from stool and gut samples.

'''Microbiome Project'''

Methods include Qiagen DNA isolation kits

2021-09-16T01:25:06Z

Rileybigham: /* DNeasy PowerWater Sterivex Kit */

Methods include Qiagen DNA isolation kits

2021-09-16T01:21:35Z

Rileybigham: /* QIAamp PowerFecal Pro DNA Kit */

Methods include Qiagen DNA isolation kits

2021-09-16T01:15:28Z

Rileybigham: /* DNeasy PowerSoil Pro Kit */

Methods include Qiagen DNA isolation kits

2021-09-16T01:14:28Z

Rileybigham: /* DNeasy PowerSoil Pro Kit */

Methods include Qiagen DNA isolation kits

2021-09-16T00:51:06Z

Rileybigham: /* Qiagen Kit Overview */

Methods include Qiagen DNA isolation kits

2021-09-15T21:55:44Z

Rileybigham: /* Qiagen Kit Overview */

Riley E Bigham

2021-09-15T21:20:51Z

Rileybigham: /* Summer 2021 */

==Personal Information==
Class of 2023

Junior Biochemistry Major

Hometown: O'Fallon, IL

Phone: 618-207-7391

Email: Rbigham@monmouthcollege.edu

=UNDERGRADUATE RESEARCH ACTIVITIES=

==SOfIA (Summer Opportunities for Intellectual Activities)==
===August 2019===
*'''SOfIA Project''': [http://esr.monmsci.net/wiki/index.php/SOfIA_2019 The Chemistry Makerspace]

==Richard "Doc" Kieft Summer Research==
'''Advisor''': ''Dr. Bradley Sturgeon''
===Summer 2021===
*Developed methods for future microbiome research
*[[Methods_include_Qiagen_DNA_isolation_kits|Methods include Qiagen DNA isolation kits]] (Powerwater, Fecal, and Soil), [[PCR & Sequencing|PCR & Sequencing]]
*Worked with Dr. Laura Moore and her research members
*'''Future Research''': The inhabitants of an aquatic microbiome and their effect on a healthy aquatic ecosystem
*'''Final Presentation''': [[:Media:Microbiome Presentation- Riley Bigham.pptx| Understanding the Aquatic Microbiome]]
::''The microbiome is a unique set of microbes living in a particular environment. The microbes that make up the human microbiome are established at birth and evolve throughout life. These microbes are essential for our well-being by regulating our immune system, synthesizing vitamins, and protecting us against certain disease-causing bacteria. The demand for new research has risen in popularity in the past 20 years allowing new discoveries connecting the microbes to the human condition. An example is the use of fecal transplants for treating obesity. New developments have allowed us to understand cause and effects from the rich community of microbes within different environments. As with the human microbiome, aquatic systems also contain a unique collection of microbes. The goal of this project is to evaluate the microbes present in aquatic systems and to correlate the presences of these microbes to the overall health of the aquatic ecosystems.''

===Shedd Aquarium===

=Presentations=
*June 2021: '''Richard "Doc" Kieft Summer Research Program'''
:Title: ''Understanding the Aquatic Microbiome'' (Oral Presentation)

*August 2019: '''Monmouth College SOfIA Program'''
:Title: ''The Chemistry Makerspace'' (Oral and Poster Presentations)

==Career Goals==

Riley E Bigham

2021-09-15T21:20:07Z

Rileybigham: /* Richard "Doc" Kieft Summer Research */

==Personal Information==
Class of 2023

Junior Biochemistry Major

Hometown: O'Fallon, IL

Phone: 618-207-7391

Email: Rbigham@monmouthcollege.edu

=UNDERGRADUATE RESEARCH ACTIVITIES=

==SOfIA (Summer Opportunities for Intellectual Activities)==
===August 2019===
*'''SOfIA Project''': [http://esr.monmsci.net/wiki/index.php/SOfIA_2019 The Chemistry Makerspace]

==Richard "Doc" Kieft Summer Research==
'''Advisor''': ''Dr. Bradley Sturgeon''
===Summer 2021===
*Developed methods for future microbiome research
*[[Methods_include_Qiagen_DNA_isolation_kits|Methods include Qiagen DNA isolation kits]] (Powerwater, Fecal, and Soil), [[PCR, Sequencing|PCR, Sequencing]]
*Worked with Dr. Laura Moore and her research members
*'''Future Research''': The inhabitants of an aquatic microbiome and their effect on a healthy aquatic ecosystem
*'''Final Presentation''': [[:Media:Microbiome Presentation- Riley Bigham.pptx| Understanding the Aquatic Microbiome]]
::''The microbiome is a unique set of microbes living in a particular environment. The microbes that make up the human microbiome are established at birth and evolve throughout life. These microbes are essential for our well-being by regulating our immune system, synthesizing vitamins, and protecting us against certain disease-causing bacteria. The demand for new research has risen in popularity in the past 20 years allowing new discoveries connecting the microbes to the human condition. An example is the use of fecal transplants for treating obesity. New developments have allowed us to understand cause and effects from the rich community of microbes within different environments. As with the human microbiome, aquatic systems also contain a unique collection of microbes. The goal of this project is to evaluate the microbes present in aquatic systems and to correlate the presences of these microbes to the overall health of the aquatic ecosystems.''

===Shedd Aquarium===

=Presentations=
*June 2021: '''Richard "Doc" Kieft Summer Research Program'''
:Title: ''Understanding the Aquatic Microbiome'' (Oral Presentation)

*August 2019: '''Monmouth College SOfIA Program'''
:Title: ''The Chemistry Makerspace'' (Oral and Poster Presentations)

==Career Goals==

Riley E Bigham

2021-09-15T21:19:06Z

Rileybigham: /* Summer 2021 */

==Personal Information==
Class of 2023

Junior Biochemistry Major

Hometown: O'Fallon, IL

Phone: 618-207-7391

Email: Rbigham@monmouthcollege.edu

=UNDERGRADUATE RESEARCH ACTIVITIES=

==SOfIA (Summer Opportunities for Intellectual Activities)==
===August 2019===
*'''SOfIA Project''': [http://esr.monmsci.net/wiki/index.php/SOfIA_2019 The Chemistry Makerspace]

==Richard "Doc" Kieft Summer Research==
'''Advisor''': ''Dr. Bradley Sturgeon''
===Summer 2021===
*Developed methods for future microbiome research
*[[Methods_include_Qiagen_DNA_isolation_kits|Methods include Qiagen DNA isolation kits]] (Powerwater, Fecal, and Soil), [[PCR,sequencing|PCR,Sequencing]]
*Worked with Dr. Laura Moore and her research members
*'''Future Research''': The inhabitants of an aquatic microbiome and their effect on a healthy aquatic ecosystem
*'''Final Presentation''': [[:Media:Microbiome Presentation- Riley Bigham.pptx| Understanding the Aquatic Microbiome]]
::''The microbiome is a unique set of microbes living in a particular environment. The microbes that make up the human microbiome are established at birth and evolve throughout life. These microbes are essential for our well-being by regulating our immune system, synthesizing vitamins, and protecting us against certain disease-causing bacteria. The demand for new research has risen in popularity in the past 20 years allowing new discoveries connecting the microbes to the human condition. An example is the use of fecal transplants for treating obesity. New developments have allowed us to understand cause and effects from the rich community of microbes within different environments. As with the human microbiome, aquatic systems also contain a unique collection of microbes. The goal of this project is to evaluate the microbes present in aquatic systems and to correlate the presences of these microbes to the overall health of the aquatic ecosystems.''

===Shedd Aquarium===

=Presentations=
*June 2021: '''Richard "Doc" Kieft Summer Research Program'''
:Title: ''Understanding the Aquatic Microbiome'' (Oral Presentation)

*August 2019: '''Monmouth College SOfIA Program'''
:Title: ''The Chemistry Makerspace'' (Oral and Poster Presentations)

==Career Goals==

Methods include Qiagen DNA isolation kits

2021-09-15T21:16:10Z

Rileybigham:

== Qiagen Kit Overview==

QIAGEN offers molecular sample and assay technology. Some of these technologies are provided as kits. These kits utilize spin columns to isolate DNA and RNA.

'''DNA Extraction'''

'''Purification'''

'''Wash'''

'''Dry'''

'''Elution'''

==DNeasy PowerWater Sterivex Kit==

==DNeasy PowerSoil Pro Kit==

==QIAamp PowerFecal Pro DNA Kit==

Methods include Qiagen DNA isolation kits

2021-09-15T21:12:26Z

Rileybigham: /* DNeasy PowerWater Kit */

Methods include Qiagen DNA isolation kits

2021-09-15T21:12:11Z

Rileybigham:

Methods include Qiagen DNA isolation kits

2021-09-15T21:00:58Z

Rileybigham: /* Qiagen Kit Overview */

== Qiagen Kit Overview==

QIAGEN offers molecular sample and assay technology. Some of these technologies are provided as kits. These kits utilize spin columns to isolate DNA and RNA.

STEPS

Methods include Qiagen DNA isolation kits

2021-09-15T20:33:39Z

Rileybigham: Created page with "== Qiagen Kit Overview=="

== Qiagen Kit Overview==

Riley E Bigham

2021-07-27T20:43:11Z

Rileybigham: /* Personal Information */

==Personal Information==
Class of 2023

Junior Biochemistry Major

Hometown: O'Fallon, IL

Phone: 618-207-7391

Email: Rbigham@monmouthcollege.edu

=UNDERGRADUATE RESEARCH ACTIVITIES=

==SOfIA (Summer Opportunities for Intellectual Activities)==
===August 2019===
*'''SOfIA Project''': [http://esr.monmsci.net/wiki/index.php/SOfIA_2019 The Chemistry Makerspace]

==Richard "Doc" Kieft Summer Research==
'''Advisor''': ''Dr. Bradley Sturgeon''
===Summer 2021===
*Developed methods for future microbiome research
*Methods include Qiagen DNA isolation kits(Powerwater, Fecal, and Soil), PCR, Sequencing
*Worked with Dr. Laura Moore and her research members
*'''Future Research''': The inhabitants of an aquatic microbiome and their effect on a healthy aquatic ecosystem
*'''Final Presentation''': [[:Media:Microbiome Presentation- Riley Bigham.pptx| Understanding the Aquatic Microbiome]]
::''The microbiome is a unique set of microbes living in a particular environment. The microbes that make up the human microbiome are established at birth and evolve throughout life. These microbes are essential for our well-being by regulating our immune system, synthesizing vitamins, and protecting us against certain disease-causing bacteria. The demand for new research has risen in popularity in the past 20 years allowing new discoveries connecting the microbes to the human condition. An example is the use of fecal transplants for treating obesity. New developments have allowed us to understand cause and effects from the rich community of microbes within different environments. As with the human microbiome, aquatic systems also contain a unique collection of microbes. The goal of this project is to evaluate the microbes present in aquatic systems and to correlate the presences of these microbes to the overall health of the aquatic ecosystems.''
===Shedd Aquarium===

=Presentations=
*June 2021: '''Richard "Doc" Kieft Summer Research Program'''
:Title: ''Understanding the Aquatic Microbiome'' (Oral Presentation)

*August 2019: '''Monmouth College SOfIA Program'''
:Title: ''The Chemistry Makerspace'' (Oral and Poster Presentations)

==Career Goals==

Riley E Bigham

2021-07-27T15:46:59Z

Rileybigham: /* Richard "Doc" Kieft Summer Research */

==Personal Information==
Class of 2023

Junior Chemistry Major, minor in forensics

Hometown: O'Fallon, IL

Phone: 618-207-7391

Email: Rbigham@monmouthcollege.edu

=UNDERGRADUATE RESEARCH ACTIVITIES=

==SOfIA (Summer Opportunities for Intellectual Activities)==
===August 2019===
*'''SOfIA Project''': [http://esr.monmsci.net/wiki/index.php/SOfIA_2019 The Chemistry Makerspace]

==Richard "Doc" Kieft Summer Research==
'''Advisor''': ''Dr. Bradley Sturgeon''
===Summer 2021===
*Developed methods for future microbiome research
*Methods include Qiagen DNA isolation kits(Powerwater, Fecal, and Soil), PCR, Sequencing
*Worked with Dr. Laura Moore and her research members
*'''Future Research''': The inhabitants of an aquatic microbiome and their effect on a healthy aquatic ecosystem
*'''Final Presentation''': [[:Media:Microbiome Presentation- Riley Bigham.pptx| Understanding the Aquatic Microbiome]]
::''The microbiome is a unique set of microbes living in a particular environment. The microbes that make up the human microbiome are established at birth and evolve throughout life. These microbes are essential for our well-being by regulating our immune system, synthesizing vitamins, and protecting us against certain disease-causing bacteria. The demand for new research has risen in popularity in the past 20 years allowing new discoveries connecting the microbes to the human condition. An example is the use of fecal transplants for treating obesity. New developments have allowed us to understand cause and effects from the rich community of microbes within different environments. As with the human microbiome, aquatic systems also contain a unique collection of microbes. The goal of this project is to evaluate the microbes present in aquatic systems and to correlate the presences of these microbes to the overall health of the aquatic ecosystems.''
===Shedd Aquarium===

=Presentations=
*June 2021: '''Richard "Doc" Kieft Summer Research Program'''
:Title: ''Understanding the Aquatic Microbiome'' (Oral Presentation)

*August 2019: '''Monmouth College SOfIA Program'''
:Title: ''The Chemistry Makerspace'' (Oral and Poster Presentations)

==Career Goals==

Riley E Bigham

2021-07-27T15:46:41Z

Rileybigham: /* Summer 2021 */

==Personal Information==
Class of 2023

Junior Chemistry Major, minor in forensics

Hometown: O'Fallon, IL

Phone: 618-207-7391

Email: Rbigham@monmouthcollege.edu

=UNDERGRADUATE RESEARCH ACTIVITIES=

==SOfIA (Summer Opportunities for Intellectual Activities)==
===August 2019===
*'''SOfIA Project''': [http://esr.monmsci.net/wiki/index.php/SOfIA_2019 The Chemistry Makerspace]

==Richard "Doc" Kieft Summer Research==
'''Advisor''': ''Dr. Bradley Sturgeon''
===Summer 2021===
*Developed methods for future microbiome research
*Methods include Qiagen DNA isolation kits(Powerwater, Fecal, and Soil), PCR, Sequencing
*Worked with Dr. Laura Moore and her research members
*'''Future Research''': The inhabitants of an aquatic microbiome and their effect on a healthy aquatic ecosystem
*'''Final Presentation''': [[:Media:Microbiome Presentation- Riley Bigham.pptx| Understanding the Aquatic Microbiome]]
::''The microbiome is a unique set of microbes living in a particular environment. The microbes that make up the human microbiome are established at birth and evolve throughout life. These microbes are essential for our well-being by regulating our immune system, synthesizing vitamins, and protecting us against certain disease-causing bacteria. The demand for new research has risen in popularity in the past 20 years allowing new discoveries connecting the microbes to the human condition. An example is the use of fecal transplants for treating obesity. New developments have allowed us to understand cause and effects from the rich community of microbes within different environments. As with the human microbiome, aquatic systems also contain a unique collection of microbes. The goal of this project is to evaluate the microbes present in aquatic systems and to correlate the presences of these microbes to the overall health of the aquatic ecosystems.''

===Shedd Aquarium===

=Presentations=
*June 2021: '''Richard "Doc" Kieft Summer Research Program'''
:Title: ''Understanding the Aquatic Microbiome'' (Oral Presentation)

*August 2019: '''Monmouth College SOfIA Program'''
:Title: ''The Chemistry Makerspace'' (Oral and Poster Presentations)

==Career Goals==

Riley E Bigham

2021-07-27T15:45:50Z

Rileybigham: /* Presentations */

==Personal Information==
Class of 2023

Junior Chemistry Major, minor in forensics

Hometown: O'Fallon, IL

Phone: 618-207-7391

Email: Rbigham@monmouthcollege.edu

=UNDERGRADUATE RESEARCH ACTIVITIES=

==SOfIA (Summer Opportunities for Intellectual Activities)==
===August 2019===
*'''SOfIA Project''': [http://esr.monmsci.net/wiki/index.php/SOfIA_2019 The Chemistry Makerspace]

==Richard "Doc" Kieft Summer Research==
'''Advisor''': ''Dr. Bradley Sturgeon''
===Summer 2021===
*Developed methods for future microbiome research
*Methods include Qiagen DNA isolation kits(Powerwater, Fecal, and Soil), PCR, Sequencing
*Worked with Dr. Laura Moore and her research members
*'''Future Research''': The inhabitants of an aquatic microbiome and their effect on a healthy aquatic ecosystem
*'''Final Presentation''': [[:Media:Microbiome Presentation- Riley Bigham.pptx| Understanding the Aquatic Microbiome]]
::''The microbiome is a unique set of microbes living in a particular environment. The microbes that make up the human microbiome are established at birth and evolve throughout life. These microbes are essential for our well-being by regulating our immune system, synthesizing vitamins, and protecting us against certain disease-causing bacteria. The demand for new research has risen in popularity in the past 20 years allowing new discoveries connecting the microbes to the human condition. An example is the use of fecal transplants for treating obesity. New developments have allowed us to understand cause and effects from the rich community of microbes within different environments. As with the human microbiome, aquatic systems also contain a unique collection of microbes. The goal of this project is to evaluate the microbes present in aquatic systems and to correlate the presences of these microbes to the overall health of the aquatic ecosystems.''

=Presentations=
*June 2021: '''Richard "Doc" Kieft Summer Research Program'''
:Title: ''Understanding the Aquatic Microbiome'' (Oral Presentation)

*August 2019: '''Monmouth College SOfIA Program'''
:Title: ''The Chemistry Makerspace'' (Oral and Poster Presentations)

==Career Goals==

Riley E Bigham

2021-07-27T15:42:56Z

Rileybigham: /* UNDERGRADUATE RESEARCH ACTIVITIES */

==Personal Information==
Class of 2023

Junior Chemistry Major, minor in forensics

Hometown: O'Fallon, IL

Phone: 618-207-7391

Email: Rbigham@monmouthcollege.edu

=UNDERGRADUATE RESEARCH ACTIVITIES=

==SOfIA (Summer Opportunities for Intellectual Activities)==
===August 2019===
*'''SOfIA Project''': [http://esr.monmsci.net/wiki/index.php/SOfIA_2019 The Chemistry Makerspace]

==Richard "Doc" Kieft Summer Research==
'''Advisor''': ''Dr. Bradley Sturgeon''
===Summer 2021===
*Developed methods for future microbiome research
*Methods include Qiagen DNA isolation kits(Powerwater, Fecal, and Soil), PCR, Sequencing
*Worked with Dr. Laura Moore and her research members
*'''Future Research''': The inhabitants of an aquatic microbiome and their effect on a healthy aquatic ecosystem
*'''Final Presentation''': [[:Media:Microbiome Presentation- Riley Bigham.pptx| Understanding the Aquatic Microbiome]]
::''The microbiome is a unique set of microbes living in a particular environment. The microbes that make up the human microbiome are established at birth and evolve throughout life. These microbes are essential for our well-being by regulating our immune system, synthesizing vitamins, and protecting us against certain disease-causing bacteria. The demand for new research has risen in popularity in the past 20 years allowing new discoveries connecting the microbes to the human condition. An example is the use of fecal transplants for treating obesity. New developments have allowed us to understand cause and effects from the rich community of microbes within different environments. As with the human microbiome, aquatic systems also contain a unique collection of microbes. The goal of this project is to evaluate the microbes present in aquatic systems and to correlate the presences of these microbes to the overall health of the aquatic ecosystems.''

==Presentations==
*June 2021: '''Richard "Doc" Kieft Summer Research Program'''
:Title: ''Understanding the Aquatic Microbiome'' (Oral Presentation)

*August 2019: '''Monmouth College SOfIA Program'''
:Title: ''The Chemistry Makerspace'' (Oral and Poster Presentations)

==Career Goals==

Riley E Bigham

2021-07-27T15:42:08Z

Rileybigham: /* Presentations */

==Personal Information==
Class of 2023

Junior Chemistry Major, minor in forensics

Hometown: O'Fallon, IL

Phone: 618-207-7391

Email: Rbigham@monmouthcollege.edu

==UNDERGRADUATE RESEARCH ACTIVITIES==
==SOfIA (Summer Opportunities for Intellectual Activities)==
===August 2019===
*'''SOfIA Project''': [http://esr.monmsci.net/wiki/index.php/SOfIA_2019 The Chemistry Makerspace]

==Richard "Doc" Kieft Summer Research==
'''Advisor''': ''Dr. Bradley Sturgeon''
===Summer 2021===
*Developed methods for future microbiome research
*Methods include Qiagen DNA isolation kits(Powerwater, Fecal, and Soil), PCR, Sequencing
*Worked with Dr. Laura Moore and her research members
*'''Future Research''': The inhabitants of an aquatic microbiome and their effect on a healthy aquatic ecosystem
*'''Final Presentation''': [[:Media:Microbiome Presentation- Riley Bigham.pptx| Understanding the Aquatic Microbiome]]
::''The microbiome is a unique set of microbes living in a particular environment. The microbes that make up the human microbiome are established at birth and evolve throughout life. These microbes are essential for our well-being by regulating our immune system, synthesizing vitamins, and protecting us against certain disease-causing bacteria. The demand for new research has risen in popularity in the past 20 years allowing new discoveries connecting the microbes to the human condition. An example is the use of fecal transplants for treating obesity. New developments have allowed us to understand cause and effects from the rich community of microbes within different environments. As with the human microbiome, aquatic systems also contain a unique collection of microbes. The goal of this project is to evaluate the microbes present in aquatic systems and to correlate the presences of these microbes to the overall health of the aquatic ecosystems.''

==Presentations==
*June 2021: '''Richard "Doc" Kieft Summer Research Program'''
:Title: ''Understanding the Aquatic Microbiome'' (Oral Presentation)

*August 2019: '''Monmouth College SOfIA Program'''
:Title: ''The Chemistry Makerspace'' (Oral and Poster Presentations)

==Career Goals==

Riley E Bigham

2021-07-27T15:40:51Z

Rileybigham: /* Summer 2021 */

Riley E Bigham

2021-07-27T15:04:02Z

Rileybigham: /* Richard "Doc" Kieft Summer Research */